| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,499,118 | T→C | L61L (TTG→CTG) | rimL → | ribosomal‑protein‑L12‑serine acetyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,499,118 | 0 | T | C | 93.3% | 89.6 / ‑2.0 | 30 | L61L (TTG→CTG) | rimL | ribosomal‑protein‑L12‑serine acetyltransferase |
| Reads supporting (aligned to +/- strand): ref base T (1/1); new base C (14/14); total (15/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01 | |||||||||||
TGTTGCAGAAAATCACGTCAAACCTCTTTATCAGTTAATCTGTAAAAATAAAACCTGGTTACAGCAGTCGCTAAACTGGCCGCAGTTTGTTCAAAGTGAAGAGGACACGCGAAAAACGGTGCAGGGTAATGTGATGTTGCATCAACGCGGCTATGCCAAAATGTTCATGATTTTCAAAGAAGATGAACTTATCGGCGTTATCTCGTTTAATCGTATTGAACCACTGAATAAAACCGCTGAAATAGGCTACTGGCTGGACGAATCTCA > NC_000913/1498982‑1499248 | tGTTGCAGAAAATCACGTCAAACCTCTTTATCAGTTAATCTGTAAAAATAAAACCTGGTTACAGCAGTCGCTAAACTGGCCGCAGTTTGTTCAAAGTGAAGAGGACACGCGAAAAACGGTGCAGGGTAATGTGAtgctgc > 2:462698/1‑140 (MQ=255) gCAGAAAATCACGTCAAACCTCTTTATCAGTTAATCTGTAAAAATAAAACCTGGTTACAGCAGTCGCTAAACTGGCCGCAGTTTGTTCAAAGTGAAGAGGACACGCGAAAAACGGTGCAGGGTAATGTGATGCTGCATCa > 2:526677/1‑140 (MQ=255) aaTCACGTCAAACCTCTTTATCAGTTAATCTGTAAAAATAAAACCTGGTTACAGCAGTCGCTAAACTGGCCGCAGTTTGTTCAAAGTGAAGAGGACACGCGAAAAACGGTGCAGGGTAATGTGATGCTGCATCAACGCgg < 2:62478/140‑1 (MQ=255) aaTCACGTCAAACCTCTTTATCAGTAAATATGTAAAAATAAAACCTGGTTACAGCAGTCGCTAAACTGGCCGCAGTTTGTTCAAAGTGAAGAGGACACGCAAAAAACGGTGCAGGGTAATGTGATGCTGCATCAACGCgg < 1:212592/140‑1 (MQ=255) cAAACCTCTTTATTAGTCAATCGGTAAAAATAAAACCTGATTACAGCATTCGCTAAACTGGCCGCAGTTTGTTCAAAGTGAAGAGGACACGCGAAAAACGGTGCAGGGTAATGTGATGCTGCATCAACGCGGCTATGCCa < 2:280824/140‑1 (MQ=255) cAAACCTCTTTATCAGTTAATCTGTAAAAATAAAACCTGGTTACAGCAGTCGCTAAACTGGCCGCAGTTTGTTCAAAGTGAAGAGGACACGCGAAAAACGGTGCAGGGTAATGTGATGCTGCATCAACGCGGCTATGCCa < 2:589155/140‑1 (MQ=255) cAAACCTCTTTATCAGTTAATCTGTAAAAATAAAACCTGGTTACAGCAGTCGCTAAACTGGCCGCAGTTTGTTCAAAGTGAAGAGGACACGCGAAAAACGGTGCAGGGTAATGTGATGCTGCATCAACGCGGCTATGCCa > 1:433865/1‑140 (MQ=255) tctTTATCAGTTAATCTGTAAAAATAAAACCTGGTTACAGCAGTCGCTAAACTGGCCGCAGTTTGTTCAAAGTGAAGAGGACACGCGAAAAACGGTGCAGGGTAATGTGATGCTGCATCAACGCGGCTATGCCAAAATGt < 1:526677/140‑1 (MQ=255) tttATCAGTTAATCTGTAAAAATAAAACCTGGTTACAGCAGTCGCTAAACTGGCCGCAGTTTGTTCAAAGTGAAGAGGACACGCGAAAAACGGTGCAGGGTAATGTGATGTTGCATCAACGCGGCTATGCCAAAATGTTc > 1:75997/1‑140 (MQ=255) tttATCAGTTAATCTGTAAAAATAAAACCTGGTTACAGCAGTCGCTAAACTGGCCGCAGTTTGTTCAAAGTGAAGAGGACACGCGAAAAACGGTGCAGGGTAATGTGATGCTGCATCAACGCGGCTATGCCAAAATGTTc > 2:159539/1‑140 (MQ=255) aGTTAATCTGTAAAAATAAAACCTGGTTACAGCAGTCGCTAAACTGGCCGCAGTTTGTTCAAAGTGAAGAGGACACGCGAAAAACGGTGCAGGGTAATGTGATGCTGCATCAACGCGGCTATGCCAAAATGTTCATGAtt > 1:551579/1‑140 (MQ=255) aTCTGTAAAAATAAAACCTGGTTACAGCAGTCGCTAAACTGGCCGCAGTTTGTTCAAAGTGAAGAGGACACGCGAAAAACGGTGCAGGGTAATGTGATGCTGCATCAACGCGGCTATGCCAAAATGTTCATGATTTTCaa < 1:154593/140‑1 (MQ=255) tAAAAATAAAACCTGGTTACAGCAGTCGCTAAACTGGCCGCAGTTTGTTCAAAGTGAAGAGGACACGCGAAAAACGGTGCAGGGTAATGTGATGCTGCATCACCGCGGCTTTGCTAAAATGTTCGTGATTTTCAaagaag > 1:310673/1‑140 (MQ=255) tAAAAATAAAACCTGGTTACAGCAGTCGCTAAACTGGCCGCAGTTTGTTCAAAGTGAAGAGGACACGCGAAAAACGGTGCAGGGTAATGTGATGCTGCATCAACGCGGCTATGCCAAAATGTTCATGATTTTCAaagaag < 1:182909/140‑1 (MQ=255) aCCTGGTTACAGCAGTCGCTAATCTGGCCGCTGTTGGTTTAAAGTGAACAGGACACGCGAAAAACGGTGCAGGGTAATGTGATGCTGCATCAACGCGGCTATGCCAAAATGTTCATGATTTTCAAAGAAGATGAACTTAt < 2:221732/140‑1 (MQ=255) aCAGCAGTCGCTAAACTGGCCGCAGTTTGTTCAAAGTGAAGAGGACACGCGAAAAACGGTGCAGGGTAATGTGATGCTGCATCAACGCGGCTATGCCAAAATGTTCATGATTTTCAAAGAAGATGAACTTATCGGCGTTa < 1:517195/140‑1 (MQ=255) cagcagTCGCTAAACTGGCCGCAGTTTGTTCAAAGTGAAGAGGACACGCGAAAAACGGTGCAGGGTAATGTGATGCTGCATCAACGCGGCTATGCCAAAATGTTCATGATTTTCAAAGAAGATGAACTTATCGGCGTTAt < 2:688341/140‑1 (MQ=255) cagcagTCGCTAAACTGGCCGCAGTTTGTTCAAAGTGAAGAGGACACGCGAAAAACGGTGCAGGGTAATGTGATGCTGCATCAACGCGGCTATGCCAAAATGTTCATGATTTTCAAAGAAGATGAACTTATCGGCGTTAt > 1:595498/1‑140 (MQ=255) tCGCTAAACTGGCCGCAGTTTGTTCAAAGTGAAGAGGACACGCGAAAAACGGTGCAGGGTAATGTGATGCTGCATCAACGCGGCTATGCCAAAATGTTCATGATTTTCAAAGAAGATGAACTTATCGGCGTTATCTCGtt > 1:601531/1‑140 (MQ=255) tCGCTAAACTGGCCGCAGTTTGTTCAAAGTGAAGAGGACACGCGAAAAACGGTGCAGGGTAATGTGATGCTGCATCAACGCGGCTATGCCAAAATGTTCATGATTTTCAAAGAAGATGAACTTATCGGCGTTATCTCGtt > 1:183854/1‑140 (MQ=255) ggCCGCAGTTTGTTCAAAGTGAAGAGGACACGCGAAAAACGGTGCAGGGTAATGTGATGCTGCATCAACGCGGCTATGCCAAAATGTTCATGATTTTCAAAGAAGATGAACTTATCGGCGTTATCTCGTTTAATCGTAtt > 1:422376/1‑140 (MQ=255) ccGCAGTTTGTTCAAAGTGAAGAGGACACGCGAAAAACGGTGCAGGGTAATGTGATGCTGCATCAACGCGGCTATGCCAAAATGTTCATGATTTTCAAAGAACATGAACTGATCAGCATTATCTAGTTTAATCGTATTGa > 1:346743/1‑140 (MQ=255) aGTTTGTTCAAAGTGAAGAGGACACGCGAAAAACGGTGCAGGGTAATGTGATGCTGCATCAACGCGGCTATGCCAAAATGTTCATGATTTTAAAAGAAGACGAACTCATCGGCGTTACCTCGGCTAATCGTATTGAACCa > 2:342907/1‑140 (MQ=255) tttGTTCAAAGTGAAGAGGACACGCGAAAAACGGTGCAGGGTAATGTGATGCTGCATCAACGCGGCTATGCCAAAATGTTCATGATTTTCAAAGAAGATGAACTTATCGGCGTTGTCACGTTTAGTCGTATTGAACCACt > 1:340560/1‑140 (MQ=255) aaaGTGAAGAGGACACGCGAAAAACGGTGCAGGGTAATGTGATGTTGCATCAACGCGGCTATGCCAAAATGTTCATGATTTTCAAAGAAGATGAACTTATCGGCGTTATCTCGTTTAATCGTATTGAACCACTGAATaaa < 2:75997/140‑1 (MQ=255) agagGACACGCGAAAAACGGTGCAGGGTAATGTGATGCTGCATCAACGCGGCTATGCCAAAATGTTCATGATTTTCAAAGAAGATGAACTTATCGGCGTTATCTCGTTTAATCGTATTGAACCACTGAATAAAACCGCTg > 1:722013/1‑140 (MQ=255) acacGCGAAAAACGGAGCAGGGCAACGTGATGCTGCAACAACGCGGCTATGCCAAAATGTTCATGATTTTCAAAGAAGATGAACTTATCGGCGTTATCTCGTTTAATCGTATTGAACCACTGAATAAAACCGCTGAAATa < 1:342907/140‑1 (MQ=255) aaaCGGTGCAGGGTAATGTGATGCTGCATCAACGCGGCTATGCCAAAATGTTCATGATTTTCAAAGAAGATGAACTTATCGGCGTTATCTCGTTTAATCGTATTGAACCACTGAATAAAACCGCTGAAATAGGCTActgg > 1:551245/1‑140 (MQ=255) gTGCAGGGTAATGTGATGCTGCATCAACGCGGCTATGCCAAAATGTTCATGATTTTCAAAGAAGATGAACTTATCGGCGTTATCTCGTTTAATCGTATTGAACCACTGAATAAAACCGCTGAAATAGGCTACTGGCTGGa < 2:433865/140‑1 (MQ=255) cAGGGTAATGTGATGCTGCATCAACGCGGCTATGCCAAAATGTTCATGATTTTCAAAGAAGATGAACTTATCGGCGTTATCTCGTTTAATCGTATTGAACCACTGAATAAAACCGCTGAAATAGGCTACTGGCTGGACGa < 2:183854/140‑1 (MQ=255) aaTGTGATGCTGCATCAACGCGGCTATGCCAAAATGTTCATGATTTTCAAAGAAGATGAACTTATCGGCGTTATCTCGTTTAATCGTATTGAACCACTGAATAAAACCGCTGAAATAGGCTACTGGCTGGACGAATCtca < 2:595498/140‑1 (MQ=255) | TGTTGCAGAAAATCACGTCAAACCTCTTTATCAGTTAATCTGTAAAAATAAAACCTGGTTACAGCAGTCGCTAAACTGGCCGCAGTTTGTTCAAAGTGAAGAGGACACGCGAAAAACGGTGCAGGGTAATGTGATGTTGCATCAACGCGGCTATGCCAAAATGTTCATGATTTTCAAAGAAGATGAACTTATCGGCGTTATCTCGTTTAATCGTATTGAACCACTGAATAAAACCGCTGAAATAGGCTACTGGCTGGACGAATCTCA > NC_000913/1498982‑1499248 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |