Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,550,878	A→G	I140V (ATT→GTT)	fdnH →	formate dehydrogenase N subunit beta

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,550,878	0	A	G	100.0%	119.2 / NA	34	I140V (ATT→GTT)	fdnH	formate dehydrogenase N subunit beta
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (17/17);  total (17/17)

TAAAGACGGCTGTATGCACTGTGAAGATCCCGGCTGCCTGAAGGCGTGCCCGTCTGCTGGTGCAATCATTCAGTACGCTAACGGGATTGTCGATTTCCAGTCGGAAAACTGCATCGGCTGTGGTTACTGCATTGCCGGGTGTCCGTTTAATATTCCGCGCCTCAACAAAGAGGATAACCGGGTATATAAATGCACGCTCTGCGTCGATCGCGTCAGCGTCGGCCAGGAACCGGCTTGTGTGAAAACCTGTCCGACCG  >  NC_000913/1550748‑1551004                                                                                                                                   |                                                                                                                               tAAAGACGGCTGTATGCACTGTGAAGATCCCGGCTGCCTGAAGGCGTGCCCGTCTGCTGGTGCAATCATTCAGTACGCTAACGGGATTGTCGATTTCCAGTCGGAAAACTGCATCGGCTGTGGTTACTGCGTTGCCGGgt                                                                                                                       <  1:711168/140‑1 (MQ=255)   aaGACGGCTGTATGCACTGTGAAGATCCCGGCTGCCTGAAGGCGTGCCCGTCTGCTGGTGCAATCATTCAGTACGCTAACGGGATTGTCGATTTCCAGTCGGAAAACTGCATCGGCTGTGGTTACTGCGTTGCCGGgtgt                                                                                                                     <  2:502476/140‑1 (MQ=255)    aGACGGCTGTATGCACTGTGAAGATCCCGGCTGCCTGAAGGCGTGCCCGTCTGCTGGTGCAATCATTCAGTACGCTAACGGGATTGTCGATTTCCAGTCGGAAAACTGCATCGGCTGTGGTTACTGCGTTGCCGGGTGTc                                                                                                                    <  2:648834/140‑1 (MQ=255)     gACGGCTGTATGCACTGTGAAGATCCCGGCTGCCTGAAGGCGTGCCCGTCTGCTGGTGCAATCATTCAGTACGCTAACGGGATTGTCGATTTCCAGTCGGAAAACTGCATCGGCTGTGGTTACTGCGTTGCCGGGTGTcc                                                                                                                   <  1:669489/140‑1 (MQ=255)                gCACTGTGAAGATCCCGGCTGCCTGAAGGCGTGCCCGTCTGCTGGTGCAATCATTCAGTACGCTAACGGGATTGTCGATTTCCAGTCGGAAAACTGCATCGGCTGTGGTTACTGCGTTGCCGGGTGTCCGTTTAATATTc                                                                                                        >  2:125784/1‑140 (MQ=255)                    tgtgAAGATCCCGGCTGCCTGAAGGCGTGCCCGTCTGCTGGTGCAATCATTCAGTACGCTAACGGGATTGTCGATTTCCAGTCGGAAAACTGCATCGGCTGTGGTTACTGCGTTGCCGGgtgt                                                                                                                     <  1:476813/123‑1 (MQ=255)                    tgtgAAGATCCCGGCTGCCTGAAGGCGTGCCCGTCTGCTGGTGCAATCATTCAGTACGCTAACGGGATTGTCGATTTCCAGTCGGAAAACTGCATCGGCTGTGGTTACTGCGTTGCCGGgtgt                                                                                                                     >  2:476813/1‑123 (MQ=255)                      tgAAGATCCCGGCTGCCTGAAGGCGTGCCCGTCTGCTGGTGCAATCATTCAGTACGCTAACGGGATTGTCGATTTCCAGTCGGAAAACTGCATCGGCTGTGGTTACTGCGTTGCCGGGTGTCCGTTTAATATTCCgcgc                                                                                                   >  1:182680/1‑139 (MQ=255)                      tgAAGATCCCGGCTGCCTGAAGGCGTGCCCGTCTGCTGGTGCAATCATTCAGTACGCTAACGGGATTGTCGATTTCCAGTCGGAAAACTGCATCGGCTGTGGTTACTGCGTTGCCGGGTGTCCGTTTAATATTCCgcgc                                                                                                   <  2:182680/139‑1 (MQ=255)                       gAAGATCCCGGCTGCCTGAAGGCGTGCCCGTCTGCTGGTGCAATCATTCAGTACGCTAACGGGATTGTCGATTTCCAGTCGGAAAACTGCATCGGCTGTGGTTACTGCGTTGCCAGGCGTCCGTCTAATATTCCGCGCCt                                                                                                 >  1:307321/1‑140 (MQ=255)                            tCCCGGCTGCCTGAAGGCGTGCCCGTCTGCTGGTGCAATCATTCAGTACGCTAACGGGATTGTCGATTTCCAGTCGGAAAACTGCATCGGCTGTGGTTACTGCGTTGCCGGGTGTCCGTTTAATATTCCGCGCCTcaaca                                                                                            <  2:10705/140‑1 (MQ=255)                                ggCTGCCTGAAGGCGTGCCCGTCTGCTGGTGCAATCATTCAGTACGCTAACGGGATTGTCGATTTCCAGTCGGAAAACTGCATCGGCTGTGGTTACTGCGTTGCCGGGTGTCCGTTTAATATTCCGCGCCTCAACAAaga                                                                                        >  2:567830/1‑140 (MQ=255)                                ggCTGCCTGAAGGCGTGCCCGTCTGCTGGTGCAATCATTCAGTACGCTAACGGGATTGTCGATTTCCAGTCGGAAAACTGCATCGGCTGTGGTTACTGCGTTGCCGGGTGTCCGTTTAATATTCCGCGCCTCAACAAaga                                                                                        >  1:737105/1‑140 (MQ=255)                                      cTGAAGGCGTGCCCGTCTGCTGGTGCAATCATTCAGTACGCTAACGGGATTGTCGATTTCCAGTCGGAAAACTGCATCGGCTGTGGTTACTGCGTTGCCGGGTGTCCGTTTAATATTCCGCGCCTCAACAAAGAGGATaa                                                                                  <  2:737105/140‑1 (MQ=255)                                       tGAAGGCGTGCCCGTCTGCTGGTGCAATCATTCAGTACGCTAACGGGATTGTCGATTTCCAGTCGGAAAACTGCATCGGCTGTGGTTACTGCGTTGCCGGGTGTCCGTTTAATATTCCGCGCCTCAACAAAGAGGATAAc                                                                                 >  2:546395/1‑140 (MQ=255)                                            gCGTGCCCGTCTGCTGGTGCAATCATTCAGTACGCTAACGGGATTGTCGATTTCCAGTCGGAAAACTGCATCGGCTGTGGTTACTGCGTTGCCGGGTGTCCGTTTAATATTCCGCGCCTCAACAAAGAGGATAACCgggg                                                                            >  2:74939/1‑139 (MQ=255)                                               tGCCCGTCTGCTGGTGCAATCATTCAGTACGCTAACGGGATTGTCGATTTCCAGTCGGAAAACTGCATCGGCTGTGGTTACTGCGTTGCCGGGTGTCCGTTTAATATTCCGCGCCTCAACAAAGAGGATAACCGGGtata                                                                         >  1:565086/1‑140 (MQ=255)                                               tGCCCGTCTGCTGGTGCAATCATTCAGTACGCTAACGGGATTGTCGATTTCCAGTCGGAAAACTGCATCGGCTGTGGTTACCGCGTTGCCGGGTGTCCGTTTAATATTCCGCGCCTCAACAAAGAGGATAACCGGGtata                                                                         >  1:65586/1‑140 (MQ=255)                                                  ccGTCTGCTGGTGCAATCATTCAGTACGCTAACGGGATTGTCGATTTCCAGTCGGAAAACTGCATCGGCTGTGGTTACTGCGTTGCCGGGTGTCCGTTTAATATTCCGCGCCTCAACAAAGAGGATAACCgg                                                                              >  1:20048/1‑132 (MQ=255)                                                  ccGTCTGCTGGTGCAATCATTCAGTACGCTAACGGGATTGTCGATTTCCAGTCGGAAAACTGCATCGGCTGTGGTTACTGCGTTGCCGGGTGTCCGTTTAATATTCCGCGCCTCAACAAAGAGGATAACCgg                                                                              <  2:20048/132‑1 (MQ=255)                                                       tgctgGTGCAATCATTCAGTACGCTAACGGGATTGTCGATTTCCAGTCGGAAAACTGCATCGGCTGTGGTTACTGCGTTGCCGGGTGTCCGTTTAATATTCCGCGCCTCAACAAAGAGGATAACCGGGTATATAAATGCa                                                                 <  1:546395/140‑1 (MQ=255)                                                        gctgGTGCAATCATTCAGTACGCTAACGGGATTGTCGATTTCCAGTCGGAAAACTGCATCGGCTGTGGTTACTGCGTTGCCGGGTGTCCGTTTAATATTCCGCGCCTCAACAAAGAGGATAACCGGGTATATAAATGCAc                                                                <  1:567830/140‑1 (MQ=255)                                                              gCAATCATTCAGTACGCTAACGGGATTGTCGATTTCCAGTCGGAAAACTGCATCGGCTGTGGTTACTGCGTTGCCGGGTGTCCGTTTTATATTCCGCGCCTCAACAAAGAGGATAACCGGGTATATAAATGCACGctctt                                                          >  1:534075/1‑139 (MQ=255)                                                                             gCTAACGGGATTGTCGATTTCCAGTCGGAAAACTGCATCGGCTGTGGTTACTGCGTTGCCGGGTGTCCGTTTAATATTCCGCGCCTCAACAAAGAGGATAACCGGGTATATAAATGCACGCTCTGCGTCGATCGCGTCAg                                           >  1:425760/1‑140 (MQ=255)                                                                              cTAACGGGATTGTCGATTTCCAGTCGGAAAACTGCATCGGCTGTGGTTACTGCGTTGCCGGGTGTCCGTTTAATATTCCGCGCCTCAACAAGGAGGATAAGCGGGTATGTAAATGCAGGCTCGGCGTCGATCGCGTCAGc                                          >  2:199874/1‑140 (MQ=255)                                                                               tAACGGGATTGTCGATTTCCAGTCGGAAAACTGCATCGGCTGTGGTTATTGCGTTGCCGGGTGTCCGTTTAATATTCCGCGCCTCAACAAAGAGGATAACCGGGTATATAAATGCACGCTCTGCGTCGATCGCGTCAGCg                                         <  2:368227/140‑1 (MQ=255)                                                                               tAACGGGATTGTCGATTTCCAGTCGGAAAACTGCATCGGCTGTGGTTACTGCGTTGCCGGGTGTCCGTTTAATATTCCGCGCCTCAACAAAGAGGATAACCGGGTATATAAATGCACGCTCTGCGTCGATCGCGTCAGCg                                         >  1:159856/1‑140 (MQ=255)                                                                                    ggATTGTCGATTTCCAGTCGGAAAACTGCATCGGCTGTGGTTACTGCGTTGCCGGGTGTCCGTTTAATATTCCGCGCCTCAACAAAGAGGATAACCGGGTATATAAATGCACGCTCTGCGTCGATCGCGTCAGCGTCGGc                                    >  2:581540/1‑140 (MQ=255)                                                                                    ggATTGTCGATTTCCAGTCGGAAAACTGCATCAGCTGTGGTTACTGCGTTGCCGGGTGTCCGTTTAATATTCCGCGCCTCAACAAAGAGGATAACCGGGTATATAAATGCACGCTCTGCGTCGATCGCGTCAGTGTCGGc                                    >  2:443270/1‑140 (MQ=255)                                                                                     gATTGTCGATTTCCAGTCGGAAAACTGCATCGGCTGTGGTTACCGCGTTGCCGGGTGTCCGTTTAATATTCCGCGCCTCAACAAAGAGGATAACCGGGTATATAAATGCACGCTCTGCGTCGATCGCGTCAGCGTCGGcc                                   <  2:65586/140‑1 (MQ=255)                                                                                                 ccAGTCGGAAAACTGCATCGGCTGTGGTTACTGCGTTGCCGGGTGTCCGTTTAATATTCCGCGCCTCAACAAAGAGGATAACCGGGTATATAAATGCACGCTCTGCGTCGATCGCGTCAGCGTCGGCCAGGAACCGGCtt                       <  2:159856/140‑1 (MQ=255)                                                                                                               gCATCGGCTGTGGTTACTGCGTTGCCGGGTGTCCGTTTAGTATTCCGCACCTCAACAAAGAGGATAACCGGGTATATAAATGCACGCTCTGCGTCGATCGCGTCAGCGTCGGCCAGGAACCGGCTTGTGTGAAAACCTGt         <  2:307321/140‑1 (MQ=255)                                                                                                               gCATCGGCTGTGGTTACTGCGTTGCCGGGTGTCCGTTTAATATTCCGCGCCTCAACAAAGAGGATAACCGGGTATATAAATGCACGCTCTGCGTCGATCGCGTCAGCGTCGGCCAGGAACCGGCTTGTGTGAAAACCTGt         <  1:125784/140‑1 (MQ=255)                                                                                                                      cTGTGGTTACTGCGTCGCCGGGCGTACGTATAATATTTCGCGCCTCAACAAAGAGGATAACCGGGTATATAAATGCACGCTCTGCGTCGATCGCGTCAGCGTCGGCCAGGAACCGGCTTGTGTGAAAACCTGTCCGACCg  <  1:199874/140‑1 (MQ=255)                                                                                                                                   |                                                                                                                               TAAAGACGGCTGTATGCACTGTGAAGATCCCGGCTGCCTGAAGGCGTGCCCGTCTGCTGGTGCAATCATTCAGTACGCTAACGGGATTGTCGATTTCCAGTCGGAAAACTGCATCGGCTGTGGTTACTGCATTGCCGGGTGTCCGTTTAATATTCCGCGCCTCAACAAAGAGGATAACCGGGTATATAAATGCACGCTCTGCGTCGATCGCGTCAGCGTCGGCCAGGAACCGGCTTGTGTGAAAACCTGTCCGACCG  >  NC_000913/1550748‑1551004

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑