| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 361,682 | Δ1 bp | coding (179/612 nt) | lacA ← | galactoside O‑acetyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 361,679 | 0 | T | . | 95.2% | 74.1 / ‑1.8 | 21 | coding (182/612 nt) | lacA | galactoside O‑acetyltransferase |
| Reads supporting (aligned to +/- strand): ref base T (1/0); new base . (7/13); total (8/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.81e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCAATCAGTACGTTATCACCGATTGTTACCGTGTAGTCATCGACAATGGTTAAATTGAAATTTGCATAAAAATTGCGGCCTATATGGATGTTGGAACCGTAAGAGAAATAGACAGGCGGTTCTACCCAGGCGTTTTCCCCTACCGTGGCAAACATTTCTTTAATCAGGCTTTCTCTTTTTTCAACTTCTGATGGATGCGAGTGATTAAACTCATACATTAACGTTTTCCCACGAAGTCTTTTTTCCGGTAAGCCTTCGCA > NC_000913/361547‑361806 | gCAATCAGTACGTTATCACCGATTGTTACCGTGTAGTCATCGACAATGGTTAAATTGAAATTTGCATAAAAATTGCGGCCTATATGGATGTTGGAACCGTAAGAGAAATAGACAGGCGGTTCTACCCAGGCGt < 2:465307/133‑1 (MQ=255) gTACGTTATCACCGATTGTTACCGTGTAGTCATCGACAATGGTTAAATTGAAATTTGCATAAAAATTGCGGCCTATATGGATGTTGGAACCGTAAGAGAAATAGACAGGCGGTTCTACCCCAGCGTTTCcccc > 1:137424/1‑133 (MQ=255) gTACGTTATCACCGATTGTTACCGTGTAGTCATCGACAATGGTTAAATTGAAATTTGCATAAAAATTGCGGCCTATATGGATGTTGGAACCGTAAGAGAAATAGACAGGCGGTTCTACCCAGGCGTTTcccct > 2:566776/1‑132 (MQ=255) ttATCACCGATTGTTACCGTGTAGTCATCGACAATGGTTAAATTGAAATTTGCATAAAAATTGCGGCCTATATGGATGTTGGAACCGTAAGAGAAATAGACAGGCGGTTCTACCCAGGCG‑TTTCCCCTACCGt > 2:566595/1‑133 (MQ=255) cACCGATTGTTACCGTGTAGTCATCGACAATGGTTAAATTGAAATTTGCATAAAAATTGCGGCCTATATGGATGTTGGAACCGTAAGAGAAATAGACAGGCGGTTCTACCCAGGCG‑TTTCCCCTACCGTGGCa > 1:369205/1‑133 (MQ=255) cACCGATTGTTACCGTGTAGTCATCGACAATGGTTAAATTGAAATTTGCATAAAAATTGCGGCCTATATGGATGTTGGAACCGTAAGAGAAATAGACAGGCGGTTCTAACAAGGAG‑TATCCCCTGCCGCGGCa > 1:301777/1‑133 (MQ=255) gATTGTTACCGTGTACTCTTCGCCAATGGTTAAATTGAAATTTGCATAAAAATTGCGGCCTATATGGATGTTGGAACCGTAAGAGAAATAGACAGGCGGTTCTACCCAGGCG‑TTTCCCCTACCGTGGCAAACa < 1:260735/133‑1 (MQ=255) tGTTACCGTGTAGTCATCGACAATGGTTAAATTGAAATTTGCATAAAAATTGCGGCCTATATGGATGTTGGAACCGTAAGAGAAATAGACAGGCGGTTCTAACCAGGCGGTTT‑CCCTACCGTGGGAAAAAttt > 2:120406/1‑133 (MQ=255) ttACCGTGTAGTCATCGACAATGGTTAAATTGAAATTTGCATAAAAATTGCGGCCTATATGGATGTTGGAACCGTAAGAGAAATAGACAGGCGGTTCTACCCCGAGGTTTT‑CCCTACCGGGGCAAACAttttt > 2:346401/1‑131 (MQ=255) aTCGACAATGGTTAAATTGAAATTTGCATAAAAATTGCGGCCTATATGGATGTTGGAACCGTAAGAGAAATAGACAGGCGGTTTTATCCAGGCG‑GTTCCCCTACCGCGGCAAAAATTTCTTTAATTAGGCttt > 2:535998/1‑133 (MQ=255) tCGACAATGGTTAAATTGAAATTTGCATAAAAATTGCGGCCTATATGGATGTTGGAACCGTAAGAGAAATAGACAGGCGGTTCTACCCAGGCG‑TTTCCCCTACCGTGGCAAACCTTTCTTTAATCCGGCTTtc < 2:369205/133‑1 (MQ=255) tGGTTAAATTGAAATTTGCATAAAAATTGCGGCCTATATGGATGTTGGAACCGTAAGAGAAATAGACAGGCGGTTCTACCCAGGCG‑TTTCCCCTACCGTGGCAAACATTTCTTTAATCAGGCTTTCTCttttt > 2:495670/1‑133 (MQ=255) ttGAAATTTGCATAAAAATTGCGGCCTATATGGATGTTGGAACCGTAAGAGAAATAGACAGGCGGTTCTACCCAGGCG‑TTTCCCCTACCGTGGCAAACATTTCTTTAATCAGGCTTTCTCTTTTTTCAACTTc < 1:132569/133‑1 (MQ=255) gtaaTTTGCATAAAACTTGCGGCCTAAAAGGATGTTGGAACCGTAAGAGAAATAGACAGGCGGTTCTACCCAGGCG‑TTTCCCCTACCGTGGCAAACATTTCTTTAATCAGGCTTTCTCTTTTTTCAACTTCTg < 2:301777/131‑1 (MQ=255) gAAATTTGCATAAAAATTGCGGCCTATATGGATGTTGGAACCGTAAGAGAAATAGACAGGCGGTTCTACCCAGGCG‑TTTCCCCTACCGTGGCAAACATTTCTTTAATCAGGCTTTCTCTTTTTTCAACTTCTg < 1:566595/133‑1 (MQ=255) gAAATTTGCATAAAAATTGCGGCCTATATGGATGTTGGAACCGTAAGAGAAATAGACAGGCGGTTCTACCCAGGCG‑TTTCCCCTACCGTGGCAAACATTTCTTTAATCAGGCTTTCTCTTTTTTCAACTTCTg < 1:120406/133‑1 (MQ=255) tGCATAAAAATTGCGGCCTATATGGATGTTGGAACCGTAAGAGAAATAGACAGGCGGTTCTACCCAGGCG‑TTTCCCCTACCGTGGCAAACATTTCTTTAATCAGGCTTTCTCTTTTTTCAACTTCTgatggat < 2:5038/133‑1 (MQ=255) tGCATAAAAATTGCGGCCTATATGGATGTTGGAACCGTAAGAGAAATAGACAGGCGGTTCTACCCAGGCG‑TTTCCCCTACCGTGGCAAACATTTCTTTAATCAGGCTTTCTCTTTTTTCAACTTCTgatggat < 1:346401/133‑1 (MQ=255) aTAAAAATTGCGGCCTATATGGATGTTGGAACCGTAAGAGAAATAGACAGGCGGTTCTACCCAGGCG‑TTTCCCCTACCGTGGCAAACATTTCTTTAATCAGGCTTTCTCTTTTTTCAACTTCTGATGGATGCg < 1:566776/133‑1 (MQ=255) aTTGCGGCCTATATGGATGTTGGAACCGTAAGAGAAATAGACAGGCGGTTCTACCCAGGCG‑TTTCCCCTACCGTGGCAAACATTTCTTTAATCAGGCTTTCTCTTTTTTCAACTTCTGATGGATGCGAGTGAt < 1:495670/133‑1 (MQ=255) agatatGGATGTTGGAACCGTAAGAGAAATAGACAGGCGGTTCTACCCAGGCG‑TTTCCCCTACCGTGGCAAACATTTCTTTAATCAGGCTTTCTCTTTTTTCAACTTCTGATGGATGCGAGTGATTAAACTCa < 2:147091/131‑1 (MQ=255) tGTTGGAACCGTAAGAGAAATAGACAGGCGGTTCTACCCAGGCG‑TTTCCCCTACCGTGGCAAACATTTCTTTAATCAGGCTTTCTCTTTTTTCAACTTCTGATGGATGCGAGTGATTAAACTCATACATTAAc > 1:84298/1‑133 (MQ=255) ggAACCGTAAGAGAAATAGACAGGCGGTTCTACCCAGGCG‑TTTCCCCTACCGTGGCAAACATTTCTTTAATCAGGCTTTCTCTTTTTTCAACTTCTGATGGATGCGAGTGATTAAACTCATACATTAACGttt > 1:569931/1‑133 (MQ=255) ggAACCGTAAGAGAAATAGACAGGCGGTTCTACCCAGGCG‑TTTCCCCTACCGGGGCAAACATTTCTTTTATCAGGCTTTTTCTTTTTTTAACTTCTGATGGATGCGAGGGGTTAAACTCATACATTAACGttt > 1:413100/1‑133 (MQ=255) tAAGAGAAATAGACAGGCGGTTCTACCCAGGCGTTTTCCCC‑ACCGTGGCAAACATTTCTTTAATCAGGCTTTCTCTTTTTTTAACTTCTGATGGATGCGAGTGATTAAACTCATACATTAACGTTTTCCCACg > 2:513791/1‑133 (MQ=255) aTAGACAGGCGGTTCTACCCAGGCG‑TTTCCCCTACCGTGGCAAACATTTTTTTAATCAGGCTTTCTCTTTTTTCAACTTCTGATGGATGCGAGTGATTAAACTCATACATAAACGTTTTTCCACGAAGTCttt > 1:244520/1‑133 (MQ=255) cGGTTCTACCCAGGCG‑TTTCCCCTACCGTGGCAAACATTTCTTTAATCAGGCTTTCTCTTTTTTCAACTTCTGATGGATGCGAGTGATTAAACTCATACATTAACGTTTTCCCACGAAGTCTTTTTTCCGGTa < 2:84298/133‑1 (MQ=255) cAGGCG‑TTTCCCCTACCGTGGCAAACATTTCTTTAATCAGGCTTTCTCTTTTTTCAACTTCTGATGGATGCGAGTGATTAAACTCATACATTAACGTTTTCCCACGAAGTCTTTTTTCCGGTAAGCCTTCGca < 2:413100/133‑1 (MQ=255) | GCAATCAGTACGTTATCACCGATTGTTACCGTGTAGTCATCGACAATGGTTAAATTGAAATTTGCATAAAAATTGCGGCCTATATGGATGTTGGAACCGTAAGAGAAATAGACAGGCGGTTCTACCCAGGCGTTTTCCCCTACCGTGGCAAACATTTCTTTAATCAGGCTTTCTCTTTTTTCAACTTCTGATGGATGCGAGTGATTAAACTCATACATTAACGTTTTCCCACGAAGTCTTTTTTCCGGTAAGCCTTCGCA > NC_000913/361547‑361806 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |