| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 799,720 | (A)6→5 | coding (856/954 nt) | ybhD ← | putative DNA‑binding transcriptional regulator YbhD |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 799,715 | 0 | A | . | 93.3% | 158.4 / 7.4 | 45 | coding (861/954 nt) | ybhD | putative DNA‑binding transcriptional regulator YbhD |
| Reads supporting (aligned to +/- strand): ref base A (2/1); new base . (19/23); total (21/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.92e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01 | |||||||||||
CGCTGATTTACCCGGCGCAGTCTCTCCTGCGCCGGTGTATTAACCTATCTCCTGTAACGCGTGTCTCTGGCGTTCGACGATATTGGTCCACAAATTGTCTTTATCGTCAGTCCATAAATTAATCAGCAAGGCAAAAAAGCGTTCTGCTGCTGGAGAAAGTACGGCATCTTTACGGCGAATTATTCCCAATGTTCGACGTATAACGGGTTCCACCAATGGGATACCAATAATCGAAGAATAGGGCGCATGAGGCATTGCCA > NC_000913/799583‑799842 | cGCTGATTTACCCGGCGCAGTCTCTCCTGCGCCGGTGTATTAACCTATCTCCTGTAACGCGTGTCTCTGGCGTTCGACGATATTGGTCCACAAATTGTCTTTATCGTCAGTCCATAAATTAATCAGCAAGGCa > 1:449082/1‑133 (MQ=255) cTGATTTACCCGGCGTAGTCTCGCCTGCGCTGGTGTATTAACCTATCTCCTGTAACGCGTGTCTCTGGCGTTCGACGATATTGGTCCACAAATTGTCTTTATCGTCAGTCCATAAATTAATCAGCAAGGCaaa < 1:253347/133‑1 (MQ=255) ttACCCGGCGCAGTCTCTCCTGCGCCGGTGTATTAACCTATCTCCTGTAACGCGTGTCTCTGGCGTTCGACGATATTGGTCCACAAATTGTCTTTATCGTCAGTCCATAAATTAATCAGCAAGGCaaaaagcg < 1:104599/133‑4 (MQ=255) cccGGCGCAGTCTCTCCTGCGCCGGTGTATTAACCTATCTCCTGTAACGCGTGTCTCTGGCGTTCGACGATATTGGTCCACAAATTGTCTTTATCGTCAGTCCATAAATTGATCGGCGAGGC‑AAGAAGCGTTc > 1:185420/1‑133 (MQ=255) ggCGCAGTCTCTCCTGCGCCGGTGTATTAACCTATCTCCTGTAACGCGTGTCTCTGGCGTTCGACGATATTGGTCCACAAATTGTCTTTATCGTCAGTCCATAAATTAATAAGCCAGGCAAAAAA‑CGTTctgc > 1:258328/1‑133 (MQ=255) cgcAGTCTCTCCTGCGCCGGTGTATTAACCTATCTCCTGTAACGCGTGTCTCTGGCGTTCGACGATATTGGTCCACAAATTGTCTTTATCGTCAGTCCATAAATTAATCAGCAAGGC‑AAAAAGCGTTctgctg < 1:156369/133‑1 (MQ=255) ctcCTGCGCCGGTGTCTTAACCTACCTCTTGTCACGCGTGTCTCTGGCGTTCGACGATATTGGTCCACAAATTGTCTTTATCGTCAGTCCATAAATTAATCAGCAAGGC‑AAAAAGCGTTCTGCTGCTGGAGaa < 2:185420/133‑1 (MQ=255) ccTGCGCCGGTGTATTAACCTATCTCCTGTAACGCGTGTCTCTGGCGTTCGACGATATTGGTCCACAAATTGTCTTTATCGTCAGTCCATAAATTAATCAGCAAGGC‑AAAAAGCGTTCTGCTGCTGGAGAAAg > 1:397004/1‑133 (MQ=255) gcCGGTGTATTAACCTATCTCCTGTAACGCGTGTCTCTGGCGTTCGACGATATTGGTCCACAAATTGTCTTTATCGTCAGTCCATAAATTAATCAGCAAGGC‑AAAAAGCGTTCTGCTGCTGGAGAAAGTACgg > 1:77739/1‑133 (MQ=255) cGGTGTATTAACCTATCTCCTGTAACGCGTGTCTCTGGCGTTCGACGATATTGGTCCACAAATTGTCTTTATCGTCAGTCCATAAATTAATCAGCAAGGC‑AAAAAGCGTTCTGCTGCTGGAGAAAGTACGGCa > 1:603926/1‑133 (MQ=255) aCCTATCTCCTGTAACGCGTGTCTCTGGCGTTCGACGATATTGGTCCACAAATTGTCTTTATCGTCAGTCCATAAATTAATCAGCAAGGC‑AAAAAGCGTTCTGCTGCTGGAGAAAGTACGGCATCTTTACGGc < 2:570476/133‑1 (MQ=255) tctcCTGTAACGCGTGTCTCTGGCGTTCGACGATATTGGTCCACAAATTGTCTTTATCGTCAGTCCATAAATTAATCAGCAAGGC‑AAAAAGCGTTCTGCTGCTGGAGAAAGTACGGCATCTTTACGGc > 2:574652/1‑128 (MQ=255) tctcCTGTAACGCGTGTCTCTGGCGTTCGACGATATTGGTCCACAAATTGTCTTTATCGTCAGTCCATAAATTAATCAGCAAGGC‑AAAAAGCGTTCTGCTGCTGGAGAAAGTACGGCATCTTTACGGc < 1:574652/128‑1 (MQ=255) tctcCTGTAACGCGTGTCTCTGGCGTTCGACGATATTGGTCCACAAATTGTCTTTATCGTCAGTCCATAAATTAATCAGCAAGGC‑AAAAAGCGTTCTGCTGCTGGAGAAAGTACGGCATCTTTACGGCGAatt > 1:571123/1‑133 (MQ=255) tctcCTGTAACGCGTGTCTCTGGCGTTCGACGATATTGGTCCACAAATTGTCTTTATCGTCAGTCCATAAATTAATCAGCAAGGC‑AAAAAGCGTTCTGCTGCTGGAGAAAGTACGGCATCTTTACGGCGAatt > 1:101760/1‑133 (MQ=255) tGTAACGCGTGTCTCTGGCGTTCGATGATATTGGTCCACAAATTGTCTTTATCGTCAGTCCATAAATTAATCAGCAAGGC‑AAAAAGCGTTCTGCTGCTGGAGAAAGTACGGCATCTTTACGGCGAATTATTcc > 2:433342/1‑133 (MQ=255) tGTAACGCGTGTCTCTGGCGTTCGACGATATTGGTCCACAAATTGTCTTTATCGTCAGTCCATAAATTAATCAGCAAGGCAAAAAAGCGTTCTGCTGCTGGAGAAAGTACGGCATCTTTACGGCGAATTATTc > 1:568642/1‑133 (MQ=255) tGTAACGCGCGTCTCTGGCGTTCGACGATATTGGTCCACAAATTGTCTTTATCGTCAGTCCATAAATTAATCAGCAAGGC‑AAAAAGCGTTCTGCTGCTGGAGAAAGTACGGCATCTTTACGGCGAATTATTcc > 2:463663/1‑133 (MQ=255) cgTGTCTCTGGCGTTCGACGATATTGGTCCACAAATTGTCTTTATCGTCAGTCCATAAATTAATCAGCAAGGC‑AAAAAGCGTTCTGCTGCTGGAGAAAGTACGGCATCTTTACGGCGAATTATTCCCAATGtt < 1:579185/133‑1 (MQ=255) cgTGTCTCTGGCGTTCGACGATATTGGTCCACAAATTGTCTTTATCGTCAGTCCATAAATTAATCAGCAAGGC‑AAAAAGCGATCTGCTGCTCGAGAAAGTCCGGCATCGTTACGGCGAATTAGTCCCAATGtt > 2:252159/1‑133 (MQ=255) tgtCTCTGGCGTTCGACGATATTGGTCCACAAATTGTCTTTATCGTCAGTCCATAAATTAATCAGCAAGGC‑AAAAAGCGTTCTGCTGCTGGAGAAAGTACGGCATCTTTACGGCGAATTATTCCCAATGTTCg > 2:429083/1‑133 (MQ=255) tgtCTCTGGCGTTCGACGATATTGGTCCACAAATTGTCTTTATCGTCAGTCCATAAATTAATCAGCAAGGC‑AAAAAGCGTTCTGCTGCTGGAGAAAGTACGGCATCTTTACGGCGAATTATTCCCAATGTTCg < 2:603926/133‑1 (MQ=255) tgtCTCTGGCGTTCGACGATATTGGTCCACAAATTGTCTTTATCGTCAGTCCATAAATTAATCAGCAAGGC‑AAAAAGCGTTCTGCTGCTGGAGAAAGTACGGCAACTTAACGGCGAATTATTCCCAATGTTCg > 2:276121/1‑133 (MQ=255) gtCTCTGGCGTTCGACGATATTGGTCCACAAATTGTCTTTATCGTCAGTCCATAAATTAATCAGCAAGGC‑AAAAAGCGTTCTGCTGCTGGAGAAAGTACGGCATCTTTACGGCGAATTATTCCCAATGTTCGa < 2:101760/133‑1 (MQ=255) tctgctgTTCGACGATATTGGTCCATAAATTGTCTTTATCGTCAGTCCATAAATTAATCAGCAAGGC‑AAAAAGCGTTCTGCTGCTGGAGAAAGTACGGCATCTTTACGGCGAATTATTCCCAATGTTCGACGt < 2:397004/127‑1 (MQ=255) gCGTTCGACGATATTGGTCCACAAATTGTCTTTATCGTCAGTCCATAAAATAATCAGCAAGGC‑AAAAAGCGTTCTGCTGCTGGAGAAAGTACGGCATCTTTACGGCGAATTATTCCCAATGTTCGACGTATaa < 2:584975/133‑1 (MQ=255) gTTCGACGATATTGGTCCACAATTTGTCTTTATCGTCAGTCCATAAATTAATCAGCAAGGC‑AAAAAGCGTTCTGCTGCTGGAGAAAGTACGGCATCTTTACGGCGAATTATTCCCAATGTTCGACGTATAACg < 1:252159/133‑1 (MQ=255) ttCGACGATATTGGTCCACAAATTGTCTTTATCGTCAGTCCATAAATTAATCAGCAAGGC‑AAAAAGCGTTCTGCTGCTGGAGAAAGTACGGCATCTTTACGGAGAATTATACCCAATGTTCGACGTATAACgg > 1:180067/1‑133 (MQ=255) cgacgaTATTGGTCCACAAATTGTCTTTATCGTCAGTCCATAAATTAATCAGCAAGGC‑AAAAAGCGTTCTGCTGCTGGAGAAAGTACGGCATCTTTACGGCGAATTATTCCCAATGTTCGACGTATAACGGGt > 2:375816/1‑133 (MQ=255) gacgaTATTGGTCCACAAATTGTCTTTATCGTCAGTCCATAAATTAATCAGCAAGGCAAAAAAGCGTTCTGCTGCTGGAGAAAGTACGGCATCTTTACGGCGAATTATTCCCAATGTTCGACGTATAACGGGt < 2:568642/133‑1 (MQ=255) ttGGTCCACAAATTGTCTTTATCGTCAGTCCATAAATTAATCAGCAAGGC‑AAAAAGCGTTCTGCTGCTGGAGAAAGTACGGCATCTTTACGGCGAATTATTCCCAATGTTCGACGTATAACGGGTTCCACCaa < 1:541577/133‑1 (MQ=255) tGGTCCACAACTTGTCTTTATCGTCAGTCCATCAATTAATCTGCAAGGC‑AAAAAGCGTTCTGCTGCTGGAGAAAGTACGGCATCTTTACGGCGAATTATTCCCAATGTTCGACGTATAACGGGTTCCACCAAt < 2:180067/133‑1 (MQ=255) tGGTCCACAAATTGTTTTTATCGTCAGTTCATAAATTAATCAGCAAGGC‑AAAAAGCGTTCTGCTGCTGGAGAAAGTACGGCATCTTTACGGCGAATTATTCCCAATGTTCGACGTATAACGGGTTCCACCAAt < 2:271882/133‑1 (MQ=255) tCCACAAATTGTCTTTATCGTCAGTCCATAAATTAATCAGCAAGGC‑AAAAAGCGTTCTGCTGCTGGAGAAAGTACGGCATCTTTACGGCGAATTATTCCCAATGTTCGACGTATAACGGGTTCCACCAATggg < 1:433342/133‑1 (MQ=255) ccACAAATTGTCTTTATCGTCAGTCCATAAATTAATCAGCAAGGC‑AAAAAGCGTTCTGCTGCTGGAGAAAGTACGGCATCTTTACGGCGAATTATTCCCAATGTTCGACGTATAACGGGTTCCACCAATGGGa < 2:119340/133‑1 (MQ=255) ttGTCTTTATCGTCAGTCCATAAATTAATCAGCAAGGC‑AAAAAGCGTTCTGCTGCTGGAGAAAGTACGGCATCTTTACGGCGAATTATTCCCAATGTTCGACGTATAACGGGTTCCACCAATGGGATACCaat < 2:77739/133‑1 (MQ=255) ttGTCTTTATCGTCAGTCCATAAATTAATCAGCAAGGC‑AAAAAGCGTTCTGCTGCTGGAGAAAGTACGGCATCTTTACGGCGAATTATTCCCAATGTTCGACGTATAACGGGTTCCACCAATGGGATACCaat < 1:375816/133‑1 (MQ=255) gTCTTTATCGTCAGTCCATAAATTAATCAGCAAGGC‑AAAAAGCGTTCTGCTGCTGGAGAAAGTACGGCATCTTTACGGCGAATTATTCCCAATGTTCGACGTATAACGGGTTCCACCAATGGGATACCaataa < 2:571123/133‑1 (MQ=255) gTCTTTATCGTCAGTCCATAAATTAATCAGCAAGGC‑AAAAAGCGTTCTGCTGCTGGAGAAAGTACGGCATCTTTACGGCGAATTATTCCCAATGTTCGACGTATAACGGGTTCCACCAATGGGATACCaataa > 2:580450/1‑133 (MQ=255) aGTCCATAAATTAATCAGCAAGGC‑AAAAAGCGTTCTGCTGCTGGAGAAAGTACGGCATCTTTACGGCGAATTATTCCCAATGTTCGACGTATAACGGGTTCCACCAATGGGATACCAATAATCGAAGAATAgg < 1:340188/133‑1 (MQ=255) tCCATAAATTAATCAGCAGGGC‑CAAAAGCGTTCTGCTGCTGGAGAAAGTACGGCATCTTTACGGCGAATTATTCCCAATGTTCGACGTATAACGGGTTCCACCAATGGGATACCAATAATCGAAGAATAGGgc < 1:272751/133‑1 (MQ=255) tCCATAAATTAATCAGCAAGGC‑AAAAAGCGTTCTGCTGCTGGAGAAAGTACGGCATCTTTACGGCGAATTATTCCCAATGTTCGACGTATAACGGGTTCCACCAATGGGATACCAATAATCGAAGAATAGGgc < 2:515550/133‑1 (MQ=255) tAATCAGCAAGGC‑AAAAAGCGTTCTGCTGCTGGAGAAAGTACGGCATCTTTACGGCGAATTATTCCCAATGTTCGACGTATAACGGGTTCCACCAATGGGATACCAATAATCGAAGAATAGGGCGCATGAGGc < 2:449082/133‑1 (MQ=255) aaTCAGCAAGGC‑AAAAAGCGTTCTGCTGCTGGAGAAAGTACGGCATCTTTACGGCGAATTATTCCCAATGTTCGACGTATAACGGGTTCCACCAATGGGATACCAATAATCGAAGAATAGGGCGCATGAGGCa > 1:393516/1‑133 (MQ=255) aaTCAGCAAGGC‑AAAAAGCGTTCTGCTGCTGGAGAAAGTACGGCATCTTTACGGCGAATTATTCCCAATGTTCGACGTATAACGGGTTCCAACAATGGGATACCAATACTCGAAGAAGAGGGCGCATGAGGCa > 2:272751/1‑133 (MQ=255) tCAGCAAGGC‑AAAAAGCGTTCTGCTGCTGGAGAAAGTACGGCATCTTTACGGCGAATTATTCCCAATGTTCGACGTATAACGGGTTCCACCAATGGGATACCAATAATCGAAGAATAGGGCGCATGAGGCAtt > 1:544316/1‑133 (MQ=255) gCAAGGC‑AAAAAGCGTTCTGCTGCTGGAGAAAGTACGGCATCTTTACGGCGAATTATTCCCAATGTTCGACGTATAACGGGTTCCACCAATGGGATACCAATAATCGAAGAATAGGGCGCATGAGGCATTgcc < 1:580450/133‑1 (MQ=255) cAAGGC‑AAAAAGCGTTCTGCTGCTGGAGAAAGTACGGCATCTTTACGGCGAATTATTCCCAATGTTCGACGTATAACGGGTTCCACCAATGGGATACCAATAATCGAAGAATAGGGCGCATGAGGCATTgcca > 2:524619/1‑133 (MQ=255) | CGCTGATTTACCCGGCGCAGTCTCTCCTGCGCCGGTGTATTAACCTATCTCCTGTAACGCGTGTCTCTGGCGTTCGACGATATTGGTCCACAAATTGTCTTTATCGTCAGTCCATAAATTAATCAGCAAGGCAAAAAAGCGTTCTGCTGCTGGAGAAAGTACGGCATCTTTACGGCGAATTATTCCCAATGTTCGACGTATAACGGGTTCCACCAATGGGATACCAATAATCGAAGAATAGGGCGCATGAGGCATTGCCA > NC_000913/799583‑799842 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |