| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 932,340 | (A)8→7 | intergenic (‑290/‑255) | trxB ← / → lrp | thioredoxin reductase/DNA‑binding transcriptional dual regulator Lrp |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 932,333 | 0 | A | . | 96.7% | 114.0 / ‑2.0 | 30 | intergenic (‑283/‑262) | trxB/lrp | thioredoxin reductase/DNA‑binding transcriptional dual regulator Lrp |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base . (13/16); minor base T (0/1); total (13/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ATTTCCCGCTTTATAAGCCGATTAAATGATGAATAAACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAGAAAAAAAACAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTACCGTGCTAGTGAAATCTACGTATGGCGTGGACAGACGCCATTCGTGATGTCGATAGCTGCCACAAGGCAAC > NC_000913/932204‑932457 | aTTTCCCGCTTTATAAGCCGATTAAATGATGAATAAACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAGaaaa > 1:158345/1‑133 (MQ=255) tCCCGCTTTATAAGCCGATTAAATGATGAATAAACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAGaaaaaaa < 1:493480/133‑1 (MQ=255) tCCCGCTTAATAAGCTGACTAAATGATGAAGAAACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAGaaaaaaa < 1:304280/133‑1 (MQ=255) ccGCTTTATAAGCCGATTAAATGATGAATAAACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAGaaaaaaaca > 1:44334/1‑131 (MQ=255) ccGCTTTATAAGCCGATTAAATGATGAATAAACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAGaaaaaaaca < 2:44334/133‑3 (MQ=255) ccGATTAAATGATGAATAAACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAG‑AAAAAAACAGTATTCTTatat < 1:544699/133‑1 (MQ=255) aTTAAATGATTAATAGACGCCCCTGTTAATGATTATCTGGCGTGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAG‑AAAAAAACAGTATTCTTATATgcg < 2:245937/133‑1 (MQ=255) aTTAAATGATGAATAAACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCTAGGAAG‑AAAAACACTGTATTCATATATgcg > 1:225104/1‑133 (MQ=255) ttAAATGATGAATAAACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAG‑AAAAAAACAGTATTCTtat > 1:372241/1‑127 (MQ=255) ttAAATGATGAATAAACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAG‑AAAAAAACAGTATTCTtat < 2:372241/127‑1 (MQ=255) ttAAATGATGAATAAACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAG‑AAAAAAACAGTATTCTTATATgcgc > 2:580209/1‑133 (MQ=255) ttAAATGATGAATAAACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAG‑AAAAAAACAGTATTCTTATATgcgc > 2:50417/1‑133 (MQ=255) aaaTGATGAATAAACTCCCCTGTTTATGAATACCTGGCATGCTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAG‑AAAAAAACAGTATTCTTATATGCGCAt < 2:207601/133‑1 (MQ=255) tgatgaATAAACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAG‑AAAAAAACAGTATTCTTATATGCGCATAAc < 2:53086/133‑1 (MQ=255) gaATAAACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAG‑AAAAAAACAGTATTCTTATATGCGCATAACcatg < 1:510720/133‑1 (MQ=255) aaTAAACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAG‑AAAAAAACAGTATTCTTATATGCGCATAACcatgc > 1:597589/1‑133 (MQ=255) ccTGTTAATGAATATATGGCATGTGGAACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAG‑AAAAAAACAGTATTCTTATATGCGCATAACCATGCATGTAAATAc < 2:299666/133‑1 (MQ=255) tGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAG‑AAAAAAACAGTATTCTTATATGCGCATAACCATGCATGTAAATACCa > 1:562857/1‑133 (MQ=255) tGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAG‑AAAAAAACAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTa < 1:566412/133‑1 (MQ=255) atCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAG‑AAAAAAACAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTACCGTg < 2:562857/133‑1 (MQ=255) atCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAG‑AAAAAAACAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTACCGTg < 1:466035/133‑1 (MQ=255) tCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAG‑AAAAAAACAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTACCGTGc > 1:567484/1‑133 (MQ=255) gCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAG‑AAAAAAACAGTATTCTTATATGCGCAAAACCATGCATGTAAATACCATGATTACCGTGCTAGt < 2:158345/133‑1 (MQ=255) cTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAG‑AAAAAAACAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTACCGTGCTAGTGAAATCTACg > 2:92010/1‑133 (MQ=255) aaaaaTCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAG‑AAAAAAACAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTACCGTGCTAGTGAAATCTACGTa < 2:597589/133‑1 (MQ=255) aaTCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAG‑AAAAAAACAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTACCGTGCTAGTGAAATCTACGTATgg > 2:607514/1‑133 (MQ=255) cGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAG‑AAAAAAACAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTACCGTGCTAGTGAAATCTACGTATGGCGt > 2:406780/1‑133 (MQ=255) aCAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAG‑AAAAAAACAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTACCGTGCTAGTGAAATCTACGTATGGCGTGGACAGACGCCAttt > 1:117564/1‑132 (MQ=255) aCAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAG‑AAAAAAACAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTACCGTGCTAGTGAAATCTACGTATGGCGTGGACAGACGCCATTc > 2:368349/1‑133 (MQ=255) aTCCCCTGGTGTTTTGCGAAAACATTCGAGGAAG‑AAAAAAACAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTACCGTGCTAGTGAAATCTACGTATGGCGTGGACAGACGCCATTCGTg < 1:92010/133‑1 (MQ=255) tGGTGTTTTGCGAAAACATTCGAGGAAG‑AAAAAAACAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTACCGTGCTAGTGAAATCTACGTATGGCGTGGACAGACGCCATTCGTGATGTCg < 1:368349/133‑1 (MQ=255) ggTGTTTTGCGAAAACATTCGAGGAAG‑AAAAAAACAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTACTGTGCTAGTGAAATCTACGTATGGCGTGGACAGACGCCATTCGTGATGTCGa > 2:159555/1‑133 (MQ=255) aaaaCATTCGGGGAA‑TAAAAAAACCGTATTCTAATATGCGCGTAACCATGCATGTAAATACCATGTTTACCGTGCTAGTGAAATCTACGTATGGCGTGGACAGACGCCATTCGTGATGTCGATAGCTGCcaca < 2:225104/133‑1 (MQ=255) actCGAGGAA‑CAAAACAACAGTAGACCTATATTCGCATAACCATGCATGTAAATACCATGTTTACCGTGCTAGTGAAATCTACGTATGGCGTGGACAGACGCCATTCGTGATGTCGATAGCTGCCACAAGGCa < 2:294879/131‑1 (MQ=255) aTTCGAGGAAG‑AAAAAAACAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTACCGTGCTAGTGAAATCTACGTATGGCGTGGACAGACGCCATTCGTGATGTCGATAGCTGCCACAAGGCa < 2:567484/133‑1 (MQ=255) tCGAGGAAG‑AAAAAAACAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTACCGTGCTAGTGAAATCTACGTATGGCGTGGACAGACGCCATTCGTGATGTCGATAGCTGCCACAAGGCAAc < 1:607514/133‑1 (MQ=255) | ATTTCCCGCTTTATAAGCCGATTAAATGATGAATAAACGCCCCTGTTAATGAATATCTGGCATGTTGTACTAAAAATCGATGTTTTGCTTTGACAATCCCCTGGTGTTTTGCGAAAACATTCGAGGAAGAAAAAAAACAGTATTCTTATATGCGCATAACCATGCATGTAAATACCATGTTTACCGTGCTAGTGAAATCTACGTATGGCGTGGACAGACGCCATTCGTGATGTCGATAGCTGCCACAAGGCAAC > NC_000913/932204‑932457 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |