Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,837,377	Δ1 bp	coding (166/1536 nt)	ynjC →	putative ABC transporter membrane subunit YnjC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,837,376	0	G	.	100.0%	70.9 / NA	18	coding (165/1536 nt)	ynjC	putative ABC transporter membrane subunit YnjC
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (8/10);  total (8/10)

TGTGGGCGATGGTGGCGGTGATTTATGCACCGCTGATCCCGGCAGCTCTCACGCTGATATCGCCTGCTTTGTCGTTGACACACTGGCAGGCGTTATTTGCCGATCCACAGTTACCGCAGGCATTACTGGCAACGCTGGTGTCGACAACCATCGCGGCGGTCGGGGCATTGTTGATTGCCCTGCTGGTGATTGTGGCGCTGTGGCCTGGGCCGAAATGGCAGCGTATGTGCGCCCGTCTGCCGTGGCTG  >  NC_000913/1837249‑1837496                                                                                                                                |                                                                                                                         tgtgGGCGATGGTGGCGGTGATTTATGCACCGCTGATCCCGGCAGCTCTCACGCTGATATCGCCTGCTTTGTCGTTGACACACTGGCAGGCGTTATTTGCCGATCCACAGTTACCGCAGGCATTACT‑GCAACg                                                                                                                    <  1:15986/133‑1 (MQ=255)                               cGCTGATCGCGGCAGTTCCCACGCTGATATGGCCTGCTTTGTCGTTGACACACTGGCAGGCGTTATTTGCCGATCCACAGTTACCGCAGGCATTACT‑GCAACGCTGGTGTCGACAACCATCGCGGCGGTCggg                                                                                      <  1:201331/133‑1 (MQ=255)                               cGCTGATCCCGGCAGCTCTCACGCTGATATCGCCTGCTTTGTCGTTGACACACTGGCAGGCGTTATTTGCCGATCCACAGTTACCGCAGGCATTACT‑GCAACGCTGGTGTCGACAACCATCGCGGCGGTCggg                                                                                      <  2:9958/133‑1 (MQ=255)                                         ggCAGCTCTCACGCTGATATCGCCTGCTTTGTCGTTGACACACTGGCAGGCGTTATTTGCC‑ATCCACAGTTACCGCAGGCATTACT‑GCAACGCTGGTGTCGACAACCATCGCGGCGGTCGGGGCATTGTTGAt                                                                           >  1:642684/1‑133 (MQ=255)                                              ctctCACGTTGATATCGCATGCGTTGTCGTTGACACACTGGCAGGCGTTATTTGCCGATCCACAGTTACCGCAGGCATTACT‑GCAACGCTGGTGTCGACAACCATCGCGGCGGTCGGGGCATTGTTGATTGcc                                                                       <  1:232974/133‑1 (MQ=255)                                              ctctCACGCTGATATCGCCTGCTTTGTCGTTGACACACTGGCAGGCGTTATTTGCCGATCCACAGTTACCGCAGGCATTACT‑GCAACGCTGGTGTCGACAACCATCGCGGCGGTCGGGGCATTGTTGATTGcc                                                                       <  1:8511/133‑1 (MQ=255)                                                 tcACGCTGATATCGCCTGCTTTGTCGTTGACACACTGGCAGGCGTTATTTGCCGATCCACAGTTACCGCAGGCATTACT‑GCAACGCTGGTGTCGACAACCATCGCGGCGGTCGGGGCATTGTTGATTGCCctg                                                                    <  1:508252/133‑1 (MQ=255)                                                               ccTGCTTTGTCGTTGACACACTGGCAGGCGTTATTTGCCTATCCACAGTTACCGCAGGCATTACT‑GCAACGCTGGTGTCGACAACCATCGCGGCGGTCGGGGCATTGTTGATTGCCCTGCTGGTGATTGTGgc                                                      >  2:540220/1‑133 (MQ=255)                                                                   cTTTGTCGTTGACACACTGGCAGGCGTTATTTGCCGATCCACAGTTACCGCAGGCATTACT‑GCAACGCTGGTGTCGACAACCATCGCGGCGGTCGGGGCATTCTTGATTGCCCTACTTGTGATTATGGCGCtg                                                  >  1:263752/1‑133 (MQ=255)                                                                              acacacTGGCAGGCGTTATTTGCCGATCCACAGTTACCGCAGGCATTACT‑GCAACGCTGGTGTCGACAACCATCGCGGCGGTCGGGGCATTGTTGATTGCCCTGCTGGTGATTGTGGCGCTGTGGCCTGGGcc                                       >  1:370921/1‑133 (MQ=255)                                                                              acacacTGGCAGGCGTTATTTGCCGATCCACAGTTACCGCAGGCATTACT‑GCAACGCTGGTGTCGACAACCATCGCGGCGGTCGGGGCATTGTTGATTGCCATGCTGGTGATTGTGGCGCTGTGGCCTGGGcc                                       >  1:317706/1‑133 (MQ=255)                                                                                 cacTGGCAGGCGTTATTTGCCGATCCACAGTTACCGCAGGCATTACT‑GCAACGCTGGTGTCGACAACCATCGCGGCGGTCGGGGCATTGTTGATTGCCCTGCTGGTGATTGTGGCGCTGTGGCCTGGGCCGaa                                    <  2:456593/133‑1 (MQ=255)                                                                                      gCAGGCGTTATTTGCCGATCCACAGTTACCGCAGGCATTACT‑GCAACGCTGGTGTCGACAACCATCGCGGCGGTCGGGGCATTGTTGATTGCCCTGCTGGTGATTGTGGCGCTGTGGCCTGGGCCGAAATGGc                               >  2:124228/1‑133 (MQ=255)                                                                                        aGGCGTTATTTGCCGATCCACAGTTACCGCAGGCATTACT‑GCAACGCTGGTGTCGACAACCATCGCGGCGGTCGGGGCATTGTTGATTGCCCTGCTGGTGATTGTGGCGCTGTGGCCTGGGCCGAAATGGCAg                             <  2:370921/133‑1 (MQ=255)                                                                                             ttATTTGCCGATCCACAGTTACCGCAGGCATTACT‑GCAACGCTGGTGTCGACAACCATCGCGGCGGTCGGGGCATTGTTGATTGCCCTGCTGGTGATTGTGGCGCTGTGGCCTGGGCCGAAATGGCAGCGTAt                        <  1:124228/133‑1 (MQ=255)                                                                                                  tGCCGATCCACAGTTACCGCAGGCATTACT‑GCAACGCTGGTGTCGACAACCATCGCGGCGGTCGGGGCATTGTTGATTGCCATGCTGGTGATTGTGGCGCTGTGGCCTGGGCCGAAATGGCAGCGTATGTgcg                   <  2:317706/133‑1 (MQ=255)                                                                                                     cGATCCACAGTTACCGCAGGCATTACT‑GCAACGCTGGTGTCGACAACCATCGCGGCGGTCGGGGCATTGTTGATTGCCCTGCTGGTGATTGCGGCGCTGTCGCCAGGGGCGAAATGGAAGCGCATGTGCGccc                >  2:220652/1‑133 (MQ=255)                                                                                                                   cGCAGGCATTACT‑GCAACGCTGGTGTCGACAACCATCGCGGCGGTCGGGGCATTGTTGATTGCCCTGCTGGTGATTGTGGCGCTGTGGCCTAGGCCGAAAAGGCCGCGGATGTGCGCACGTCTGCCGTGgctg  >  2:224940/1‑133 (MQ=255)                                                                                                                                |                                                                                                                         TGTGGGCGATGGTGGCGGTGATTTATGCACCGCTGATCCCGGCAGCTCTCACGCTGATATCGCCTGCTTTGTCGTTGACACACTGGCAGGCGTTATTTGCCGATCCACAGTTACCGCAGGCATTACTGGCAACGCTGGTGTCGACAACCATCGCGGCGGTCGGGGCATTGTTGATTGCCCTGCTGGTGATTGTGGCGCTGTGGCCTGGGCCGAAATGGCAGCGTATGTGCGCCCGTCTGCCGTGGCTG  >  NC_000913/1837249‑1837496

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑