| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,385,853 | (T)8→9 | intergenic (+52/+4) | yhcN → / ← yhcO | DUF1471 domain‑containing stress‑induced protein YhcN/putative barnase inhibitor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,385,845 | 1 | . | T | 88.9% | 108.7 / 6.2 | 36 | intergenic (+44/+12) | yhcN/yhcO | DUF1471 domain‑containing stress‑induced protein YhcN/putative barnase inhibitor |
| Reads supporting (aligned to +/- strand): ref base . (1/3); new base T (20/12); total (21/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.87e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AAGGCGCAACGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑T‑T‑TTT‑TTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTCCCAGATGAACAAATTCAA > NC_000913/3385725‑3385972 | aaGGCGCAACGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑t‑t‑ttt‑ttttgcg < 1:530422/133‑5 (MQ=255) gcgcAACGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTT‑T‑TTT‑TTTGCGCTa < 2:6335/133‑1 (MQ=255) cgcAACGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTT‑T‑TTT‑TTTGCGCTaa > 2:609428/1‑133 (MQ=255) cGGCCTACCAGATTACTGTAGCTCGTAGCGCTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTT‑T‑TTT‑TTTGCGCTAATGACg < 1:187128/133‑1 (MQ=255) tACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTT‑T‑TTT‑TTTGCGCTAATGACGAACATTAAAACGc > 2:388349/1‑133 (MQ=255) tACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑T‑T‑TTTCTTTTAGCTAATGGCGAAGATTAAAAccc > 2:299560/1‑131 (MQ=255) cTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTT‑T‑TTT‑TTTGCGCTAATGACGAACATTAAAACGCaa > 2:435495/1‑133 (MQ=255) cTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTT‑T‑TTT‑TTTGCTCTAATGAGGAAAATCAAAACGAAAATGccc > 1:202663/1‑133 (MQ=255) cTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTT‑T‑TTT‑TTTGCGCTAATGACGAACATTAAAACGCAAATGccc > 1:124318/1‑133 (MQ=255) tAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTT‑T‑TTT‑TTTGCGCTAATGACGAACATTCAAACGCAAAAGCCCTTcc > 1:212987/1‑133 (MQ=255) tAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTT‑T‑TTT‑TTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTcc > 1:437026/1‑133 (MQ=255) tAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTT‑T‑TTT‑TTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTcc > 1:613400/1‑133 (MQ=255) ggTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTT‑T‑TTT‑TTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGct < 2:437026/133‑1 (MQ=255) ggTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTT‑T‑TTT‑TTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGct < 2:473961/133‑1 (MQ=255) ggTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTT‑T‑TTT‑TTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGct < 1:351176/133‑1 (MQ=255) tGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTT‑T‑TTT‑TTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGctct < 1:388349/133‑1 (MQ=255) tGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTT‑T‑TTT‑TTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGctct > 1:535725/1‑133 (MQ=255) tGACACCTAGCACGCGACTGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTT‑T‑TTT‑TTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGctct < 1:299560/133‑1 (MQ=255) ggCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTT‑T‑TTT‑TTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGc > 2:558953/1‑133 (MQ=255) aCGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTT‑T‑TTT‑TTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCaa < 1:435495/133‑1 (MQ=255) gTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTT‑T‑TTT‑TTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATAtt > 1:477657/1‑133 (MQ=255) gTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTT‑T‑TTT‑TTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCA‑CTCTTCCTCTGCCTCATCAAACAGCAATATTa > 2:427459/1‑133 (MQ=255) tACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTT‑T‑TTT‑TTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTa > 2:566560/1‑133 (MQ=255) cccTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTT‑T‑TTT‑TTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGGCTCATCAAACAGCATTATTAATGCGCCaaa > 1:217893/1‑133 (MQ=255) cccTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTT‑T‑TTT‑TTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAGACAGCATTATTAAGGCGCCaaa > 1:187036/1‑133 (MQ=255) cccTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTT‑T‑TTT‑TTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCaaa > 2:333660/1‑133 (MQ=255) cccTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTT‑T‑TTT‑TTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCaaa > 2:357663/1‑133 (MQ=255) cATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑T‑T‑TTT‑TTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGc < 1:107637/133‑1 (MQ=255) cGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTT‑T‑TTT‑TTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGa > 2:330388/1‑133 (MQ=255) gTCCGACGATATTGCCCCCGGTTCGGGGGCTT‑T‑TTT‑TTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGTAATATTAGCGCTCCGAAACGGGGACGCGtt > 1:192766/1‑133 (MQ=255) cgacgaTATTTCCCCCGGTTCGGGTGC‑TCT‑TTC‑TTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTtc < 2:166001/133‑1 (MQ=255) ttGCCCCCGGGGCGGTGGCTT‑T‑TTT‑TTTTCGCTAATGACGATCATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTCCCAg < 2:202663/133‑1 (MQ=255) ccGGTTCGGGTGC‑T‑TATTT‑TTTGCCCTATTGATGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTCCCAGATGAAc < 2:187036/133‑1 (MQ=255) ggTTCGGGGGCTT‑T‑TTT‑TTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGCCAACCCGTTTTCTCTCCCAGATGAAcca > 2:305787/1‑131 (MQ=255) ggggCTT‑T‑TTT‑TTTGGGCTAATGACGAACATTTAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTCCCAGATGAACAAATTCaa < 2:212987/133‑1 (MQ=255) ggggCTT‑T‑TTT‑TTTGCGCTAATGAGGAACATTCAAACGCAAGTGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTCCCAGATGAACAAATTCaa < 2:217893/133‑1 (MQ=255) ggggCTT‑T‑TTT‑TTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTCCCAGATGAACAAATTCaa < 2:613400/133‑1 (MQ=255) | AAGGCGCAACGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGC‑T‑T‑TTT‑TTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTCCCAGATGAACAAATTCAA > NC_000913/3385725‑3385972 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |