| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,118,621 | (A)7→8 | coding (107/246 nt) | zapB → | cell division factor ZapB |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,118,614 | 1 | . | A | 95.7% | 156.0 / ‑0.7 | 48 | coding (100/246 nt) | zapB | cell division factor ZapB |
| Reads supporting (aligned to +/- strand): ref base . (2/0); new base A (27/18); total (29/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.17e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TCCATCAACGCCTTGCAATTCAGGAGAGGTATGACAATGTCATTAGAAGTGTTTGAGAAACTGGAAGCAAAAGTACAGCAGGCGATTGATACCATCACTCTGTTGCAGATGGAAATCGAAGAGCTGAAAG‑AAAA‑AAA‑CAACTCACTGTCGCAGGAAGTTCAAAATGCCCAGCATCAGCGCGAAGAGCTGGAGCGTGAGAACAACCATCTGAAAGAACAGCAGAACGGCTGGCAGGAACGTCTGCAGG > NC_000913/4118485‑4118731 | tCCATCAACGACTTGCAATTCAGGCGAGGTATAACAATGTCATTAGAAGTGTTTGAGAAACTGGAAGCAAAAGTACAGCAGGCGATTGATACCATCACTCTGTTGCAGATGGAAATCGAAGAGCTgaaag‑aaa < 2:222989/133‑1 (MQ=255) aTCAACGCCTTGCAATTCAGGAGAGGTATGACAATGTCATTAGAAGTGCTTGAGAAACTGGAAGCAAAAGTACAGCAGGCGATTGATACCATCACTCTGTTGCAGATGGAAATCGAAGAGCTGAAAG‑aaaa‑aa > 1:411508/1‑133 (MQ=255) cAACGCCTTGCAATTCAGGAGAGGTATGACAATGTCATTAGAAGTGTTTGAGAAACTGGAAGCAAAAGTACAGCAGGCGATTGATACCATCACTCTGTTGCAGATGGAAATCGAGGAGCTGAAAG‑aaaa‑aaaa > 1:178504/1‑132 (MQ=255) cAACGCCTTGCAATTCAGGAGAGGTATGACAATGTCATTAGAAGTGTTTGAGAAACTGGAAGCAAAAGTACAGCAGGCGATTGATACCATCACTCTGTTGCAGATGGAAATCGAAGAGCTgaaag‑aaa < 1:233384/128‑1 (MQ=255) cAACGCCTTGCAATTCAGGAGAGGTATGACAATGTCATTAGAAGTGTTTGAGAAACTGGAAGCAAAAGTACAGCAGGCGATTGATACCATCACTCTGTTGCAGATGGAAATCGAAGAGCTgaaag‑aaa > 2:233384/1‑128 (MQ=255) ttGCAATTCAGGAGAGGTATGACAATGTCATTAGAAGTGTTTGAGAAACTGGAAGCAAAAGTACAGCAGGCGATTGATACCATCACTCTGTTGCAGATGGAAATCGAAGAGCTGAAAGAAAAA‑AAA‑CAACTCa < 1:37577/133‑1 (MQ=255) ttGCAATTCAGGAGAGGTATGACAATGTCATTAGAAGTGTTTGAGAAACTGGAAGCAAAAGTACAGCAGGCGATTGATACCATCACTCTGTTGCAGATGGAAATCGAAGAGCTGAAAGAAAAA‑AAA‑CAACTCa < 1:110911/133‑1 (MQ=255) agGTATGACAATGTCATTAGAAGTGTTTGAGAAACTGGAAGCAAAAGTACAGCAGGCGATTGATACCATCACTCTGTTGCAGATGGAAATCAAAGAGCTGAAAG‑AAAATAAA‑CAACTGACTGTTGCAGGAAGt > 2:298415/1‑133 (MQ=255) gTATGACAATGTCATTAGAAGTGTTTGAGAAACTGGAAGCAAAAGTACAGCAGGCGATTGATACCATCACTCTGTTGCAGATGGAAATCGAAGAGCTGAAAGAAAAA‑AAA‑CAACTCACTGTCGCAGGAAGTTc > 1:155816/1‑133 (MQ=255) gACAATGTAATTAGAAGTATTTGAGAAACTTGAAGCAAAAGTACAGCAGGCGATTGATACCATCACTCTGTTGCAGATGGAAATCGAAGAGCTGAAAGAAAAA‑AAA‑CAACTCACTGTCGCAGGAAGTTCaaaa < 1:228793/133‑1 (MQ=255) aCAATGTCATTAGAAGTGTTTGAGAAACTGGAAGCAAAAGTACAGCAGGCGATTGATACCATCACTCTGTTGCAGATGGAAATCGAAGAGCTGAAAGAAAAA‑AAA‑CAACTCACTGTCGCAGGAAGTTCAAAAt > 2:386857/1‑133 (MQ=255) aCAATGTCATTAGAAGTGTTTGAGAAACTGGAAGCAAAAGTACAGCAGGCGATTGATACCATCACTCTGTTGCAGATGGAAATCGAAGAGCTGAAAG‑AAAA‑AAACCCAATCACTGTCTCAGGCCGTTCaaagt > 2:291580/1‑131 (MQ=255) aTGTCATTAGAAGTGTTTGAGAAACTGGAAGCAAAAGTACAGCAGGCGATTGATACCATCACTCTGTTGCAGATGGAAATCGAAGAGCTGAAAGAAAAA‑AAA‑CAACTCACTGTCGCAGGAAGTTCAAAATGcc < 1:386857/133‑1 (MQ=255) aTGTCATTAGAAGTGCTTGAGAAATTGGCAGCAAAAGTACAGCAGGCGATTGATACCATCACTCTGTTGCAGATGGAAATCGAAGAGCTGAAAGAAAAA‑AAA‑CAACTCACTGTCGCAGGAAGTTCAAAATGcc < 2:178504/133‑1 (MQ=255) tGTCATTAGAAGTGTTTGAGAAACTGGAAGCAAAAGTACAGCAGGCGATTGATACCATCACTCTGTTGCAGATGGAAATCGAAGAGCTGAAAGAAAAA‑AAA‑CAACTCACTGTCGCAGGAAGTTCAAAATGccc > 1:454076/1‑133 (MQ=255) aTTAGAAGTGTTTGAGAAACTGGAAGCAAAAGTACAGCAGGCGATTGATACCATCACTCTGTTGCAGATGGAAATCGAAGAGCTGAAAGAAAAA‑AAA‑CAACTCACTGTCGCAGGAAGTTCAAAATGCCCAGCa > 1:425047/1‑133 (MQ=255) aTTAGAAGTGTTTGAGAAACTGGAAGCAAAAGTACAGCAGGCGATTGATACCATCACTCTGTTGCAGATGGAAATCGAAGAGCTGAAAGAAAAA‑AAA‑CAACTCACTGTCGCAGGAAGTTCAAAATGCCCAGCa < 1:335201/133‑1 (MQ=255) aGAAGTGTTTGAGAAACTGGAAGCAAAAGTACAGCAGGCGATTGATACCATCACTCTGTTGCAGATGGAAATCGAAGAGCTGAAAGAAAAA‑AAA‑CAACTCACTGTCGCAGAAAGATCCAAATGCCCAGCATCa > 1:284309/1‑133 (MQ=255) aGTGTTTGAGAAACTGGAAGCAAAAGTACAGCAGGCGATTGATACCATCACTCTGTTGCAGATGGAAATCGAAGAGCTGAAAGAAAAA‑AAA‑CAACTCACTGTCGCAGGAAGTTCAAAATGCCCAGCATCAgcg > 2:130418/1‑133 (MQ=255) tgtTTGAGAAACTGGAAGCAAAAGTACAGCAGGCGATTGATACCATCACTCTGTTGCAGATGGAAATCGAAGAGCTGAAAGAAAAA‑AAA‑CAACTCACTGTCGCAGGAAGTTCAAAATGCCCAGCATCAGcgcg < 2:397667/133‑1 (MQ=255) tgtTTGAGAAACTGGAAGCAAAAGTACAGCAGGCGATTGATACCATCACTCTGTTGCAGATGGAAATCGAAGAGCTGAAAGAAAAA‑AAA‑CAACTCACTGTCGCAGGAAGTTCAAAATGCCCAGCATCAGcgcg > 1:604215/1‑133 (MQ=255) tttGAGAAACTGGAAGCAAAAGTACAGCAGGCGATTGATACCATCACTCTGTTGCAGATGGAAATCGAAGAGCTGAAAGAAAAA‑AAA‑CAACTCACTGTCGCTGGAAGTTCAAAATGCCCAGCATCAGcgcg > 1:78172/1‑131 (MQ=255) tttGAGAAACTGGAAGCAAAAGTACAGCAGGCGATTGATACCATCACTCTGTTGCAGATGGAAATCGAAGAGCTGAAAGAAAAA‑AAA‑CAACTCACTGTCGCTGGAAGTTCAAAATGCCCAGCATCAGcgcg < 2:78172/131‑1 (MQ=255) tttGAGAAACTGGAAGCAAAAGTACAGCAGGCGATTGATACCATCACTCTGTTGCAGATGGAAATCGAAGAGCTGAAAGAAAAA‑AAA‑CAACTCACTGTCGCAGGAAGTTCAAAATGCCCAGCATCAGCGCGaa < 2:368935/133‑1 (MQ=255) tGAGAAACTGGAAGCAAAAGTACAGCAGGCGATTGATACCATCACTCTGTTGCAGATGGAAATCGAAGAGCTGAAAGAAAAA‑AAA‑CAACTCCCTGTCGCAGGAAGTTCAAAATGCCCGGCATCAGCGCGAaga > 2:227740/1‑133 (MQ=255) tGAGAAACTGGAAGCAAAAGTACAGCAGGCGATTGATACCATCACTCTGTTGCAGATGGAAATCGAAGAGCTGAAAGAAAAA‑AAA‑CAACTCACTGTCGCAGGAAGTTCAAAATGCCCAGCATCAGCGCGAaga > 1:101694/1‑133 (MQ=255) agaAACTGGAAGCAAAAGTACAGCAGGCGATTGATACCATCACTCTGTTGCAGATGGAAATCGAAGAGCTGAAAGAAAAA‑AAA‑CAACTCACTGTCGCAGGAAGTTCAAAATGCCCAGCATCAGCGCGAAGAGc < 2:373013/133‑1 (MQ=255) tGGAAGCAGAAGTACAGCAGGCGATTGATACCATCACTCTGTTGCAGATGGAAATCGAAGAGCTGAAAGAAAAA‑AAA‑CAACTCACTGTCGCAGGAAGTTCAAAATGCCCAGCATCAGCGCGAAGAGCTGGAGc > 2:471537/1‑133 (MQ=255) tGGAAGCAAAAGTACAGCAGGCGATTGATACCATCACTCTGTTGCAGATGGAAATCGAAGAGCTGAAAGAAAAA‑AAA‑CAACTCACTGTCGCAGGAAGTTCAAAATGCCCAGCATCAGCGCGAAGAGCTGGAGc < 1:606451/133‑1 (MQ=255) tGGAAGCAAAAGTACAGCAGGCGATTGATACCATCACTCTGTTGCAGATGGAAATCGAAGAGCTGAAAGAAAAA‑AAA‑CAACTCACTGTCGCAGGAAGTTCAAAATGCCCAGCATCAGCGCGAAGAGCTGGAGc < 1:563619/133‑1 (MQ=255) cAAAAGTACAGCAGGCGATTGATACCATCACTCTGTTGCAGATGGAAATCGAAGAGCTGAAAGAAAAA‑AAA‑CAACTCACTGTCGCAGGAAGTTCAAAATGCCCAGCATCAGCGCGAAGAGCTGGAGCGTgaga > 2:447485/1‑133 (MQ=255) aaGTACAGCAGGCGATTGATACCATCACTCTGTTGCAGATGGAAATCGAAGAGCTGAAAGAAAAA‑AAA‑CAACTCACTGTCGCAGGAAGTTCAAAATGCCCAGCATCAGCGCGAAGAGCTGGAGCGTGAGaaca > 1:443548/1‑133 (MQ=255) gTACAGCAGGCGATTGATACCATCACTCTGTTGCAGATGGAAATCGAAGAGCTGAAAGAAAAA‑AAA‑CAACTCACTGTCGCAGGAAGTTCAAAATGCCCAGCATCAGCGCGAAGAGCTGGAGCGTGAGaacaac < 1:471537/133‑1 (MQ=255) gTACAGCAGGCGATTGATACCATCACTCTGTTGCAGATGGAAATCGAAGAGCTGAAAGAAAAA‑AAA‑CAACTCACTGTCGCAGGAAGTTCAAAATGCCCAGCATCAGCGCGAAGAGCTGGAGCGTGAGaacaac < 1:447485/133‑1 (MQ=255) agGCGATTGATACCATCACTCTGTTGCAGATGGAAATCGAAGAGCTGAAAGAAAAA‑AAA‑CAACTCACTGTCGCAGGAAGTTCAAAATGCCCAGCATCAGCGCGAAGAGCTGGAGCGTGAGAACAACCATCTGa < 1:8536/133‑1 (MQ=255) ggCGATTGATACCATCACTCTGTTGCAGATGGAAATCGAAGAGCTGAAAGAAAAA‑AAA‑CAACTCACTGTCGCAGGAAGTTCAAAATGCCCATCATCAGCGGGAAAAGCTGGAGCGTGAGAACAACCATCTGaa > 2:210640/1‑133 (MQ=255) ggCGATTGATACCATCACTCTGTTGCAGATGGAAATCGAAGAGCTGAAAGAAAAA‑AAA‑CAACTCACTGTCGCAGGAAGTTCAAAATGCCCAGCATCAGCGCGAAGAGCTGGAGCGTGAGAACAACCATCTGaa > 2:114128/1‑133 (MQ=255) aCCATCACTCTGTTGCAGATGGAAATCGAAGAGCTGAAAGAAAAA‑AAA‑CAACTCACTGTCGCAGGAAGTTCAAAATGCCCAGCATCAGCGCGAAGAGCTGGAGCGTGAGAACAACCATCTGAAAGAAcagcag > 2:479303/1‑133 (MQ=255) aTCACTCTGTTGCAGATGGAAATCGAAGAGCTGAAAGAAAAA‑AAA‑CAACTCACTGTCGCAGGAAGTTCAAAATGCCCAGCATCAGCGCGAAGAGCTGGAGCGTGAGAACAACCATCTGAAAGAACAGCAGAAc > 1:367402/1‑133 (MQ=255) aTCACTCTGTTGCAGATGGAAATCGAAGAGCTGAAAGAAAAA‑AAA‑CAACTCACTGTCGCAGGAAGTTCAAAATGCCCAGCATCAGCGCGAAGAGCTGGAGCGTGAGAACAACCATCTGAAAGAACAGCAGAAc > 1:122851/1‑133 (MQ=255) tCACTCTGTTGCAGATGGAAATCGAAGAGCTGAAAGAAAAA‑AAA‑CAACTCACTGTCGCAGGAAGTTCAAAATGCCCAGCATCAGCGCGAAGAGCTGGAGCGTGAGAACAACCATCTGAAAGAACAGCAGAACg > 2:89834/1‑133 (MQ=255) ctGTTGCAGATGGAAATCGAAGAGCTGAAAGAAAAA‑AAA‑CAACTCACTGTCGCAGGAAGTTCAAAATGCCCAGCATCAGCGCGAAGAGCTGGAGCGTGAGAACAACCATCTGAAAGAACAGCAGAACGGCTgg > 2:316320/1‑133 (MQ=255) tGTTGCAGATGGAAATCGAAGAGCTGAAAGAAAAA‑AAA‑CAACTCACTGTCGCAGGAAGTTCAAAATGCCCAGCATCAGCGCGAAGAGCTGGAGCGTGAGAACAACCATCTGAAAGAACAGCAGAACGGCTGGc < 1:89834/133‑1 (MQ=255) ttGCAGATGGAAATCGAAGAGCTGAAAGAAAAA‑AAA‑CAACTCACTGTCGCAGGAAGTTCAAAATGCCCAGCATCAGCGCGAAGAGCTGGAGCGTGAGAACAAGCATCTGAAAGAACATCAGAACGGCTGGCAg > 1:213312/1‑133 (MQ=255) ttGCAGATGGAAATCGAAGAGCTGAAAGAAAAA‑AAA‑CAACTCACTGTCGCAGGAAGTTCAAAATGCCCAGCATCAGCGCGAAGAGCTGGAGCGTGAGAACAACCATCTGAAAGAACAGCAGAACGGCTGGCAg > 2:323296/1‑133 (MQ=255) ttGCAGATGGAAATCGAAGAGCTGAAAGAAAAA‑AAA‑CAACTCACTGTCGCAGGAAGTTCAAAATGCCCAGCATCAGCGCGAAGAGCTGGAGCGTGAGAACAACCATCTGAAAGAACAGCAGAACGGCTGGCAg > 1:371309/1‑133 (MQ=255) tGCAGATGGAAATCGAAGAGCTGAAAGAAAAA‑AAA‑CAACTCACTGTCGCAGGAAGTTCAAAAT‑CCCAGCATCAGCGCGAAGAGCTGGAGCGTGAGAACAACCATCTGAAAGAACAGCAGAACGGCTGGCAGGa > 1:11157/1‑133 (MQ=255) ggAAATCGAAGAGCTGAAAGAAAAA‑AAA‑CAACTCACTGTCGCAGGAAGTTCAAAATGCCCAGCATCAGCGCGAAGAGCTGGAGCGTGAGAACAACCATCTGAAAGAACAGCAGAACGGCTGGCAGGAACGTCt > 2:460759/1‑133 (MQ=255) aaaTCGAAGAGCTGAAAGAAAAA‑AAA‑CAACTCACTGTCGCAGGAAGTTCAAAATGCCCAGCATCAGCGCGAAGAGCTGGAGCGTGAGAACAACCATCTGAAACAACAGCACAACGGCAGGCAGGAACGTCTGc > 1:257519/1‑133 (MQ=255) aaaTCGAAGAGCTGAAAGAAAAA‑AAA‑CAACTCACTGTCGCAGGAAGTTCAAAATGCCCAGCATCAGCGCGAAGAGATGGAGCGTGAGAACAACCATCTGAAAGAACAGCAGAACGGCTGGCAGGAACGTCTGc > 2:601518/1‑133 (MQ=255) tCGAAGAGCTGAAAGTAAAA‑AAA‑CCACTAACTATCGCAGGACGTTCAAAATGCCCAGCATCAGCGCGAAGAGCTGGAGCGTGAGAACAACCATCTGAAAGAACAGCAGAACGGCTGGCAGGAACGTCTGCAgg < 2:213312/133‑1 (MQ=255) tCGAAGAGCTGAAAGAAAAA‑AAA‑CAACTCACTGTCGCAGGAAGTTCAAAATGCCCAGCATCAGCGCGAAGAGCTGGAGCGTGAGAACAACCATCTGAAAGAACAGCAGAACGGCTGGCAGGAACGTCTGCAgg < 2:363688/133‑1 (MQ=255) tCGAAGAGCTGAAAGAAAAA‑AAA‑CAACTCACTGTCGCAGGAAGTTCAAAATGCCCAGCATCAGCGCGAAGAGCTGGAGCGTGAGAACAACCATCTGAAAGAACAGCAGAACGGCTGGCAGGAACGTCTGCAgg < 1:130418/133‑1 (MQ=255) | TCCATCAACGCCTTGCAATTCAGGAGAGGTATGACAATGTCATTAGAAGTGTTTGAGAAACTGGAAGCAAAAGTACAGCAGGCGATTGATACCATCACTCTGTTGCAGATGGAAATCGAAGAGCTGAAAG‑AAAA‑AAA‑CAACTCACTGTCGCAGGAAGTTCAAAATGCCCAGCATCAGCGCGAAGAGCTGGAGCGTGAGAACAACCATCTGAAAGAACAGCAGAACGGCTGGCAGGAACGTCTGCAGG > NC_000913/4118485‑4118731 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |