| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,385,931 | (A)7→8 | coding (228/315 nt) | yjeO → | conserved inner membrane protein YjeO |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,385,924 | 1 | . | A | 97.6% | 137.6 / ‑3.6 | 41 | coding (221/315 nt) | yjeO | conserved inner membrane protein YjeO |
| Reads supporting (aligned to +/- strand): ref base . (0/1); new base A (21/19); total (21/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.88e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TTGATGGACGCGGGATTAACAACGTCTGCGATGTGTTGATGTATCTCGAAGAAGACGATACCCGTGATGTTGGTGTGATAATGACGTTACCGCTCTTTTTCCCTTTTCTCTGGTTCGCTTTGTGGCG‑AAAAAAA‑CGCGGCTGGTTTATGTACGCCACCGCGCTGGCTATTTTCGGCTACTGGCTGTGGCAGTTTTTTCTGCGCTATCAGTTTTGTTTGTGAGCCGGATTGGTTCATCCGGCACACAAACTTACA > NC_000913/4385798‑4386051 | ttgatGGACGCGGGATTAACAACGTCTGCGATGTGTTGATGTATCTCGAAGAAGACGATACCCGTGATGTTGGTGTGATAATGACGTTACCGCTCTTTTTCCCTTTTCTCTGGTTCGCTTTGTGGCG‑aaaaaa < 1:513383/133‑1 (MQ=255) tgatGGACGCGGGATTAACAACGTCTGCGATGTGTTGATGTATCTCGAAGAAGACGATACCCGTGATGTTGGTGTGATAATGACGTTACCGCTCTTTTTCCCTTTTCTCTGGTTCGCTTTGTGGCG‑aaaaaaa > 1:580213/1‑133 (MQ=255) tgatGGACGCGGGATTAACAACGTCTGCGATGTGTTGATGTATCTCGAAGAAGACGATACCCGTGATGTTGGTGTGATAATGACGTTACCGCTCTTTTTCCCTTTTCTCTGGTTCGCTTTGTGGCG‑aaaaaaa > 1:359403/1‑133 (MQ=255) gACGCGGGATTAACAACGTCTGCGATGTGTTGATGTATCTCGAAGAAGACGATACCCGTGATGTTGGTGTGATAATGACGTTACCGCTCTTTTTCCCTTTTCTCTGGTTCGCTTTGTGGCG‑aaaaaaaacgcg < 1:456359/133‑6 (MQ=255) aCGCGGGATTAACAACGTCTGCGATGTGTTGATGTATCTCGAAGAAGACGATACCCGTGATGTTGGTGTGATAATGACGTTACCGCTCTTTTTCCCTTTTCTCTGGTTCGCTTTGTGGCGAAAAAAAA‑CGCgg < 1:562650/133‑1 (MQ=255) cgcgGGATTAACAACTTCTGCGATGTGTTGATTTATCTCGACGAAGACGATACCCGTGATGTTGGTGTGATAATGACGTTACCGCTCTTTTTCCCTTTTCTCTGGTTCGCTTTGTGGCGAAAAAAAA‑CGCGGc < 2:269262/133‑1 (MQ=255) cgcgGGATTAACAACCTCCGCGCTGTGTTGATGTATCTCGAAGAAGACGATACCCGTGATGTTGGTGTGATAATGACGTTACCGCTCTTTTTCCCTTTTCTCTGGTTCGCTTTGTGGCGAAAAAAAA‑CGCGGc < 1:179412/133‑1 (MQ=255) ggATTAACAACGTCTGCGATGTGTTGATGTATCTCGAAGAAGACGATACCCGTGATGTTGGTGTGATAATGACGTTACCGCTCTTTTTCCCTTTTCTCTGGTTCGCTTTGTGGCGAAAAAAAA‑CGCGGCTGGt < 2:561128/133‑1 (MQ=255) ggATTAACAACGTCTGCGATGTGTTGATGTATCTCGAAGAAGACGATACCCGTGATGTTGGTGCGATAATGACGTTACCGCTCTTTTTCCCTTTTCTCTGGTTCGCTTTGTGGCGAAAAAAAA‑CGCGGCTGGt < 2:493218/133‑1 (MQ=255) aTTAACAACGTCTGCGATGTGTTGATGTATCTCGAAGAAGACGATACCCGTGATGTTGGTGTGATAATGACGTTACCGCTCTTTTTCCCTTTTCTCTGGTTCGCTTTGTGGCGAAAAAAAA‑CGCGGCTGGttt < 2:584456/133‑1 (MQ=255) gTCTGCGATGTGTTGATGTATCTCGAAGAAGACGATACCCGTGATGTTGGTGTGATAATGACGTTACCGCTCTTTTTCCCTTTTCTCTGGTTCGCTTTGTGGCGAAAAAAAA‑CGCGGCTGGTTTATGTACGcc > 1:506596/1‑133 (MQ=255) gTCTGCGATGTGTTGATGTATCTCGAAGAAGACGATACCCGTGATGTTGGTGTGATAATGACGTTACCGCTCTTTTTCCCTTTTCTCTGGTTCGCTTTGTGGCGAAAAAAAA‑CGCGGCTGGTTTATGTACGcc > 1:526065/1‑133 (MQ=255) tgtTGATGGATCTCGGAGAAGAGGATACCCTTGATGTTGGTGTGATAATGACGTTACCGCTCTTTTTCCCTTTTCTCTGGTTCGCTTTGTGGCGAAAAAAAA‑CGCGGCTGGTTTATGTACGCCACCGCGCTgg < 1:274049/133‑1 (MQ=255) tctcGAAGAAGACGATACCCGTGATGTTGGTGTGATAATGACGTTACCGCTCTTTTTCCCTTTTCTCTGGTTCGCTTTGTGGCGAAAAAAAA‑CGCGGCTGGTTTATGTACGCCATCGAGCTGGCGATTTTCgg > 1:267721/1‑133 (MQ=255) tcGAAGAAGACGATACCCGTGATGTTGGTGTGATAATGACGTTACCGCTCTTTTTCCCTTTTCTCTGGTTCGCTTTGTGGCGAAAAAAAA‑CGCGGCTGGTTTATGTACGCCACCGCGCTGGCTATTTTCGGCt > 2:349964/1‑133 (MQ=255) aagaagACGATACCCGTGATGTTGGTGTGATAATGACGTTACCGCTCTTTTTCCCTTTTCTCTGGTTCGCTTTGTGGCGAAAAAAAA‑CGCGGCTGGTTTATGTACGCCACCGCGCTGGCTATTTTCGGCTACt > 1:128939/1‑133 (MQ=255) aagaagACGATACCCGTGATGTTGGTGTGATAATGACGTTACCGCTCTTTTTCCCTTTTCTCTGGTTCGCTTTGTGGCGAAAAAAAA‑CGCGGCTGGTTTATGTACGCCACCGCGCTGGCTATTTTCGGCTACt > 2:530720/1‑133 (MQ=255) aagaagACGATACCCGTGATGTTGGTGTGATAATGACGTTACCGCTCTTTTTCCCTTTTCTCTGGTTCGCTTTGTGGCGAAAAAAAA‑CCCGGCTGGGTTTTGAACCCCAAAGCGATGAGTATTTTTGGCTagt > 1:213336/1‑131 (MQ=255) cGATACCCGTGATGTTGGTGTGATAATGACGTTACCGCTCTTTTTCCCTTTTCTCTGGTTCGCTTTGTGGCGAAAAAAAA‑CGCGGCTGGTTTATGTACGCCACCGCGCTGGCTATTTTCGGCTACTGGCtgtg < 2:128939/133‑1 (MQ=255) gTGATGTTGGTGTGAGAAGGACATTACCGCTCTTTTTCCCTTTTCTCTGGTTCGCTTTGTGGCGAAAAAAAA‑CGCGGCTGGTTTATGTACGCCACCGCGCTGGCTATTTTCGGCTACTGGCTGTGGCAGtttt < 1:281116/133‑1 (MQ=255) aTGTTGGTGTGATAATGACGTTACCGCTCTTTTTCCCTTTTCTCTGGTTCGCTTTGTGGCGAAAAAAAA‑CGCGGCTGGTTTATGTACGCCACCGCGCTGGCTATTTTCGGCTACTGGCTGTGGCAGTTTTTTc > 2:346208/1‑133 (MQ=255) ggTGTGATAATGACGTTACCGCTCTTTTTCCCTTTTCTCTGGTTCGCTTTGTGGCGAAAAAAAA‑CGCGGCTGGTTTATGTACGCCACCGCGCTGGCTATTTTCGGCTACTGGCTGTGGCAGTTTTTTCTgcgc > 1:472626/1‑133 (MQ=255) ggTGTGATAATGACGTTACCGCTCTTTTTCCCTTTTCTCTGGTTCGCTTTGTGGCGAAAAAAAA‑CGCGGCTGGTTTATGTACGCCACCGCGCTGGCTATTTTCGGCTACTGGCTGTGGCAGTTTTTTCTgcgc < 1:346208/133‑1 (MQ=255) gtgtGATAATGACGTTACCGCTCTTTTTCCCTTTTCTCTGGTTCGCTTTGTGGCGAAAAAAAA‑CGCGGCTGGTTTATGTACGCCACCGCGCTGGCTATTTTCGGCTACTGGCTGTGGCAGTTTTTTCTgc > 1:403134/1‑130 (MQ=255) gtgtGATAATGACGTTACCGCTCTTTTTCCCTTTTCTCTGGTTCGCTTTGTGGCGAAAAAAAA‑CGCGGCTGGTTTATGTACGCCACCGCGCTGGCTATTTTCGGCTACTGGCTGTGGCAGTTTTTTCTgc < 2:403134/130‑1 (MQ=255) gtgATAATGACGTTACCGCTCTTTTTCCCTTTTCTCTGGTTCGCTTTGTGGCGAAAAAAAA‑CGCGGCTGGTTTATGTACGCCACCGCGCTGGCTATTTTCGGCTACTGGCTGTGGCAGTTTTTTCTGCGCTAt > 2:99460/1‑133 (MQ=255) aTAATGACGTTACCGCTCTTTTTCCCTTTTCTCTGGTTCGCTTTGTGGCGAAAAAAAA‑CGCGGCTGGTTTATGTACGCCACCGCGCTGGCTATTTTCGGCTACTGGCTGTGGCCGTTTTTTCTGCGCTTTCAg > 2:643507/1‑133 (MQ=255) aTAATGACGTTACCGCTCTTTTTCCCTTTTCTCTGGTTCGCTTTGTGGCGAAAAAAAA‑CGCGGCTGGTTTATGTACGCCACCGCGCTGGCTATTTTCGGCTACTGGCTGTGGCAGTTTTTTCTGCGCTATCAg > 1:98446/1‑133 (MQ=255) gACGTTACCGCTCTTTTTCCCTTTTCTCTGGTTCGCTTTGTGGCGAAAAAAAA‑CGCGGCTGGTTTATGTACGCCACCGCGCTGGCTATTTTCGGCTACTGGCTGTGGCAGTTTTTTCTGCGCTATCAGTtttg > 2:579766/1‑133 (MQ=255) aCGTTACCGCTCTTTTTCCCTTTTCTCTGGTTCGCTTTGTGGCGAAAAAAAA‑CGCGGCTGGTTTATGTACGCCACCGCGCTGGCTATTTTCGGCTACTGGCTGTGGCAGTTTTTTCTGCGCTATCAGTtttgt > 1:589385/1‑133 (MQ=255) gCTCTTTTTCCCTTTTCTCTGGTTCGCTTTGTGGCGAAAAAAAA‑CGCGGCTGGTTTATGTACGCCACCGCGCTGGCTATTTTCGGCTACTGGCTGTGGCAGTTTTTTCTGCGCTATCAGTTTTGTTTGTGAGc < 1:530720/133‑1 (MQ=255) ctTTTTCCCTTTTCTCTGGTTCGCTTTGTGGCGAAAAAAAA‑CGCGGCTGGTTTATGTACGCCACCGCGCTGGCTATTTTCGGCTACTGGCTGTGGCAGTTTTTTCTGCGCTATCAGTTTTGTTTGTGAGCCgg < 1:99460/133‑1 (MQ=255) tttttCCCTTTTCTCTGGTTCGCTTTGTGGCGAAAAAAAA‑CGCGGCTGGTTTATGTACGCCACCGCGCTGGCTATTTTCGGCTACTGGCTGTGGCAGTTTTTTCTGCGCTATCAGTTTTGTTTGTGAGCCGGa < 2:589385/133‑1 (MQ=255) tttttCCCTTTTCTCTGGTTCGCTTTGTGGCGAAAAAAAA‑CGCGGCTGGTTTATGTACGCCACCGCGCTGGCTATTTTCGGCTACTGGCTGTGGCAGTTTTTTCTGCGCTATCAGTTTTGTTTGTGAGCCGGa < 2:580213/133‑1 (MQ=255) tttttCCCTTTTCTCTGGTTCGCTTTGTGGCGAAAAAAAA‑CGCGGCTGGTTTATGTACGCCACCGCGCTGGCTATTTTCGGCTACTGGCTGTGGCAGTTTTTTCTGCGCTATCAGTTTTGTTTGTGAGCCGGa < 2:359403/133‑1 (MQ=255) cccTTTTCTCTGGTTCGCTTTGTGGCGAAAAAAAA‑CGCGGCTGGTTTATGTACGCCACCGCGCTGGCTATTTTCGGCTACTGGCTGTGGCAGTTTTTTCTGCGCTATCAGTTTTGTTTGTGAGCCGGATTGGt > 2:156263/1‑133 (MQ=255) cTTTTCTCTGGTTCGGTTTGTGGCC‑AAAAAAAGCGCGGCTGATTTATGTACGCCACCGCGCTGGCTATTTTCGGCTACTGGCTGTGGCAGTTTTTTCTGCGCTATCAGTTTTGTTTGTGAGCCGGATTGGTTc < 2:267721/133‑1 (MQ=255) tctcTGGTTCGCTTTGTGGCGAAAAAAAA‑CGCGGCTGGTTTATGTACGCCACCGCGCTGGCTATTTTCGGCTACTGGCTGTGGCAGTTTTTTCTGCGCTATCAGTTTTGTTTGTGAGCCGGATTGGTTCATcc < 1:579766/133‑1 (MQ=255) tCGCTTTGTGGCGAAAAAAAA‑CGCGGCTGGTTTATGTACGCCACCGCGCTGGCTATTTTCGGCTACTGGCTGTGGCAGTTTTTTCTGCGCTATCAGTTTTGTTTGTGAGCCGGATTGGTTCATCCGGcacaca < 2:506596/133‑1 (MQ=255) tCGCTTTGTGGCGAAAAAAAA‑CGCGGCTGGTTTATGTACGCCACCGCGCTGGCTATTTTCGGCTACTGGCTGTGGCAGTTTTTTCTGCGCTATCAGTTTTGTTTGTGAGCCGGATTGGTTCATCCGGcacaca < 1:349964/133‑1 (MQ=255) cGCTTTGTGGCGAAAAAAAA‑CGCGGCTGGTTTATGTACGCCACCGCGCTGGCTATTTTCGGCTACTGGCTGTGGCAGTTTTTTCTGCGCTAACAGTTTTGGTTGAGGGGCGGATTGGTTCATACGGcacacga > 2:285794/1‑131 (MQ=255) cTTTGTGGCGAAAAAAAA‑CGCGGCTGGTTTATGTACGCCACCGCGCTGGCTATTTTCGGCTACTGGCTGTGGCAGTTTTTTCTGCGCTATCAGTTTTGTTTGTGAGCCGGATTGGTTCATCCGGCACacaaac > 1:414913/1‑133 (MQ=255) tgGCGAAAAAAAA‑CGCGGCTGGTTTATGTACGCCACCGCGCTGGCTATTTTCGGCTACTGGCTGTGGCAGTTTTTTCTGCGCTATCAGTTTTGTTTGTGAGCCGGATTGGTTCATCCGGCACacaaacttaca > 1:419514/1‑133 (MQ=255) tgGCGAAAAAAAA‑CGCGGCTGGTTTATGTACGCCACCGCGCTGGCTATTTTCGGCTACTGGCTGTGGCAGTTTTTTCTGCGCTATCAGTTTTGTTTGTGAGCCGGATTGGTTCATCCGGCACacaaacttaca > 1:75787/1‑133 (MQ=255) tgGCGAAAAAAAA‑CGCGGCTGGTTTATGTACGCCACCGCGCTGGCTATTTTCGGCTACTGGCTGTGGCAGTTTTTTCTGCGCTATCAGTTTTGTTTGTGAGCCGGATTGGTTCATCCGGCACacaaacttaca > 1:92450/1‑133 (MQ=255) | TTGATGGACGCGGGATTAACAACGTCTGCGATGTGTTGATGTATCTCGAAGAAGACGATACCCGTGATGTTGGTGTGATAATGACGTTACCGCTCTTTTTCCCTTTTCTCTGGTTCGCTTTGTGGCG‑AAAAAAA‑CGCGGCTGGTTTATGTACGCCACCGCGCTGGCTATTTTCGGCTACTGGCTGTGGCAGTTTTTTCTGCGCTATCAGTTTTGTTTGTGAGCCGGATTGGTTCATCCGGCACACAAACTTACA > NC_000913/4385798‑4386051 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |