Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	795,370	(A)6→7	coding (282/774 nt)	modA →	molybdate ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	795,364	1	.	A	100.0%	77.0 / NA	23	D92E (GAT→GAA)	modA	molybdate ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base A (11/12);  total (11/12)

AGAAAGGCGTGGATGTGGTTTCTTCTTTCGCTTCGTCATCTACTCTCGCCCGTCAGATTGAAGCGGGTGCGCCTGCGGATCTGTTTATTTCTGCCGATCAGAAATGGATGGATTATGCGGTTGAT‑AAAAAAGCGATCGATACAGCTACGCGTCAGACACTGCTCGGCAATAGCCTGGTCGTTGTAGCACCGAAAGCCAGCGTGCAGAAAGATTTCACCATCGACAGCAAAACCAACTGGACTTCACTGCTGA  >  NC_000913/795240‑795491                                                                                                                              |                                                                                                                                agaAAGGCGTGGATGTGGTTTCTTCTTTCGCTTCGTCATCTACTCTCGCCCGTCAGATTGAAGCGGGTGCGCCTGCGGATCTGTTTATTTCTGCCGATCAGAAATGGATGGATTATGCGTTTGAT‑aaaaaaa                                                                                                                          >  2:412084/1‑131 (MQ=255) agaAAGGCGTGGATGTGGTTTCTTCTTTCGCTTCGTCATCTACTCTCGCCCGTCAGATTGAAGCGGGTGCGCCTGCGGATCTGTTTATTTCTGCCGATCAGAAATGGATGGATTATGCGTTTGAT‑aaaaaaa                                                                                                                          <  1:412084/132‑2 (MQ=255) agaAAGGCGTGGATGTGGTTTCTTCTTTCGCTTCGTCATCTACTCTCGCCCGTCAGATTGAAGCGGGTGCGCCTGCGGATCTGTTTATTTCTGCCGATCAGAAATGGATGGATTATGCGGTTGAT‑aaaaaaag                                                                                                                         >  1:441531/1‑131 (MQ=255)   aaaGGCGTGGATGTGGTTTCTTCTTTCGCTTCGTCATCTACTCTCGCCCGTCAGATTGAAGCGGGTGCGCCTGCGGATCTGTTTATTTCTGCCGATCAGAAATGGATGGATTATGCGGTTGAT‑aaaaaaagcg                                                                                                                       <  1:364069/133‑5 (MQ=255)     aGGCGTGGATGTGGTTTCTTCTTTCGCTTCGTCATCTACTCTCGCCCGTCAGATTGAAGCGGGTGCGCCTGCGGATCTGTTTATTTCTGCCGATCAGAAATGGATGGATTATGCGGTTGATAAAAAAAGcgat                                                                                                                      <  1:78455/133‑1 (MQ=255)     aGGCGTGGATGTGGTATCGTCTTTCGCTTCGTCATCTACTCTCGCCCGTCAGATTGAAGCGGGTGCGCCTGCGGATCTGTTTATTTCTGCCGATCAGAAATGGATGGATTATGCGGTTGATAAAAAAAGcgat                                                                                                                      <  1:297468/133‑1 (MQ=255)                 ggTTTCTTCTTTCGCTTCGTCATCTACTCTCGCCCGTCAGATTGAAGCGGGTGCGCCTGCGGATCTGTTTATTTCTGCCGATCAGAAATGGATGGATTATGCGGTTGGTAAAAAAAGCGATCGATACAGCTAc                                                                                                          <  2:434789/133‑1 (MQ=255)                         cttTCGCTTCGTCATCTACTCTCGCCCGTCAGATTGAAGCGGGTGCGCCTGCGGATCTGTTTATTTCTGCCGATCAGAAATGGATGGATTATGCGGTTGATAAAAAAAGCGATCGATACAGCTACGCGTCAGa                                                                                                  <  2:12196/133‑1 (MQ=255)                         cttTCGCTTCGTCATCTACTCTCGCCCGTCAGATTGAAGCGGGTGCGCCTGCGGATCTGTTTATTTCTGCCGATCAGAAATGGATGGATTATGCGGTTGATAAAAAAAGCGATCGATACAGCTACGCGTCAGa                                                                                                  <  2:87933/133‑1 (MQ=255)                                     cATCTACTCTCGCCCGTCAGATTGAAGCGGGTGCGCCTGCGGATCTGTTTATTTCTGCCGATCAGAAATGGATGGATTATGCGGTTGATAAAAAAAGCGATCGATACAGCTACGCGTCAGACACTGCTCGGCa                                                                                      <  1:468308/133‑1 (MQ=255)                                     cATCTACTCTAGCCCCTCAGATTGAAGCCGGTCCGCCTGCGAATCTGTTTATTTCTGCCGATCAGAAATGGATGGATTATGCGGTTGATAAAAAAAGCGATCGATACAGCTACGCGTCAGACACTGCTCGGCa                                                                                      <  2:162414/133‑1 (MQ=255)                                          aCTCTCGCCCGTCAGATTGAAGCGGGTGCGCCTGCGGATCTGTTTATTTCTGCCGATCAGAAATGGATGGATTATGCGGTTGATAAAAAAAGCGATCGATACAGCTACGCGTCAGACACTGCTCGGCAATAGc                                                                                 <  2:441531/133‑1 (MQ=255)                                             ctcGCCCGTCAGATTGAAGCGGGTGCGCCTGCGGATCTGTTTATTTCTGCCGATCAGAAATGGATGGATTATGCGGTTGATAAAAAAAGCGATCGATACAGCTACGCGTCAGACACTGCTCGGCAATAGCCTg                                                                              <  1:84974/133‑1 (MQ=255)                                              tcGCCCGTCAGATTGAAGCGGGTGCGCCTGCGGATCTGTTTATTTCTGCCGATCAGAAATGGATGGATTATGCGGTTGATAAAAAAAGCGATCGATACAGCTACGCGTCAGACAGTGGTCGGCATTAGCCTgg                                                                             >  1:162414/1‑133 (MQ=255)                                                 cccGTCAGATTGAAGCGGGTGCGCCTGCGGATCTGTTTATTTCTGCCGATCAGAAATGGATGGATTATGCGGTTGATAAAAAAAGCGATCGATACAGCTACGCGTCAGACACTGCTCGGCAATAGCCTGGTCg                                                                          >  2:623166/1‑133 (MQ=255)                                                       aGATTGAAGCGGGTGCGCCTGCGGATCTGTTTATTTCTGCCGATCAGAAATGGATGGATTATGCGGTTGATAAAAAAAGCGATCGATACAGCTACGCGTCAGACACTGCTCGGCAATAGCCTGGTCGTTGTAg                                                                    <  2:510073/133‑1 (MQ=255)                                                                    tGCGCCTGCGGATCTGTTTATTTCTGCCGATCAGAAATGGATGGATTATGCGGTTGATAAAAAAAGCGATCGATACAGCTACGCGTCAGACCCTGCTCGGGAATCGCCGGGTCGTTGCAGCACCGAAAGCCAg                                                       >  2:186109/1‑133 (MQ=255)                                                                                         ttCTGCCGATCAGAAATGGATGGATTATGCGGTTGATAAAAAAAGCGATCGATACAGCTACGCGTCAGACACTGCTCGGCAATAGCCTGGTCGTTGTAGCACCGAAAGCCAGCGTGCAGAAAGATTTCACCAt                                  >  2:478841/1‑133 (MQ=255)                                                                                            tGCCGATCAGAAATGGATGGATTATGCGGTTGATAAAAAAAGCGATCGATACAGCTACGCGTCAGACACTGCTCGGCAATAGCCTGGTCGTTGTAGCACCGAAATCCAGCGTGCAGATAGATATCACCATCGa                               >  2:293028/1‑133 (MQ=255)                                                                                              ctgATCAGAAATGGATGGATTATGCGGTTGATAAAAAAAGCGATCGATACAGCTACGCGTCAGACACTGCTCGGCAATAGCCTGGTCGTTGTAGCACCGAAAGCCAGCGTGCAGAAAGATTTCACCATCGACa                             >  1:23151/3‑133 (MQ=255)                                                                                              ccGATCAGAAATGGATGGATTATGCGGTTGATAAAAAAAGCGATCGATACAGCTACGCGTCAGACACTGCTCGGCAATAGCCTGGTCGTTGTAGCACCGAAAGCCAGCGTGCAGAAAGATTTCACCATCGACa                             >  1:102887/1‑133 (MQ=255)                                                                                                        atggatggATTATGCGGTTGATAAAAAAAGCGATCGATACAGCTACGCGTCAGACACTGCTCGGCAATAGCCTGGTCGTTGTAGCACCGAAAGCCAGCGTGCAGAAAGATTTCACCATCGACAGCAAAACCaa                   <  1:478841/133‑1 (MQ=255)                                                                                                           gatggatTATGCGGTTGATAAAAAAAGCGATCGATACAGCTACGCGTCAGACACTGCTCGGCAATAGCCTGGTCGTTGTAGCACCGAAAGCCAGCGTGCAGAAAGATTTCACCATCGACAGCAAAACCAACTg                >  2:571905/1‑133 (MQ=255)                                                                                                                atTATGCGGTTGATAAAAAAAGCGATCGATACAGCTACGCGTCAGACACTGCTCGGCAATAGCCTGGTCGTTGTAGCACCGAAAGCCAGCGTGCAGAAAGATTTCACCATCGACAGCAAAACCAACTGGACtt           >  1:130658/1‑133 (MQ=255)                                                                                                                  tATGCGGTTGATAAAAAAAGCGATCGATACAGCTACGCGTCAGACACTGCTCGGCAATAGCCTGGTCGTTGTAGCACCGAAAGCCAGCGTGCAGAAAGATTTCACCATCGACAGCAAAACCAACTGGACTTCa         <  2:130658/133‑1 (MQ=255)                                                                                                                   aTGCGGTTGATAAAAAAAGCGATCGATACAGCTACGCGTCAGACACTGCTCGGCAATAGCCTGGTCGTTGTAGCACCGAAAGCCAGCGTGCAGAAAGATTTCACCATCGACAGCAAAACCAACTGGACTTCAc        >  2:132666/1‑133 (MQ=255)                                                                                                                         ttGATAAAAAAAGCGATCGATACAGCTACGCGTCAGACACTGCTCGGCAATAGCCTGGTCGTTGTAGCACCGAAAGCCAGCGTGCAGAAAGATTTCACCATCGACAGCAAGACCGACTGGACTTGACTGCTGa  >  1:304826/1‑133 (MQ=255)                                                                                                                              |                                                                                                                                AGAAAGGCGTGGATGTGGTTTCTTCTTTCGCTTCGTCATCTACTCTCGCCCGTCAGATTGAAGCGGGTGCGCCTGCGGATCTGTTTATTTCTGCCGATCAGAAATGGATGGATTATGCGGTTGAT‑AAAAAAGCGATCGATACAGCTACGCGTCAGACACTGCTCGGCAATAGCCTGGTCGTTGTAGCACCGAAAGCCAGCGTGCAGAAAGATTTCACCATCGACAGCAAAACCAACTGGACTTCACTGCTGA  >  NC_000913/795240‑795491

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑