Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,786,326	(T)7→8	coding (787/2379 nt)	ppsA ←	phosphoenolpyruvate synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,786,319	1	.	T	100.0%	68.2 / NA	21	I265N (ATC→AAC)	ppsA	phosphoenolpyruvate synthetase
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base T (12/9);  total (12/9)

GGCCTGTTTTGCCAGTTCCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGCTGTTCCTGCGGTACGTCTTCGATTTTAACCTGCTTGCCGTGCTCCTGGGTCGGCGCGTAAACCATGCGGA‑TTTTTTTCGACCCCATGGTGCGGCGCACGATAGCCGGGCGATTCGCCGCCAGTGTCGGTTTATGCACGTAAAACTCATCCGGGTTAACCGCACCCTGCACGACCATCTCACCAAGGCCCCATGCGGA  >  NC_000913/1786195‑1786446                                                                                                                              |                                                                                                                                ggCCTGTTTTGCCAGTTCCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGCTGTTCCTGCGGTACGTCTTCGATTTTAACCTGCTTGCCGTGCTCCTGGGTCGGCGCGTAAACCATGCGGA‑tttttttt                                                                                                                         <  2:556692/133‑2 (MQ=255)   ccTGTTTTGCCAGTTCCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGCTGTTCCTGCGGTACGTCTTCGATTTTAACCTGCTTGCCGTGCTCCTGGGTCGGCGCGTAAACCATGCGGA‑ttttttttcg                                                                                                                       <  1:521985/133‑4 (MQ=255)              aGTTCCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGCTGTTCCTGCGGTACGTCTTCGATTTTAACCTGCTTGCCGTGCTCCTGGGTCGGCGCGTAAACCATGCGGATTTTTTTTCGACCCCATGGTg                                                                                                             <  2:617675/133‑1 (MQ=255)                 tCCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGCTGTTCCTGCGGTACGTCTTCGATTTTAACCTGCTTGCCGTGCTCCTGGGTCGGCGCGTAAACCATGCGGATTTTTTTTCGACCCCATGGTgcgg                                                                                                          >  1:390751/1‑133 (MQ=255)                          tcttcGTTGGTCAGCGAGAAGATGTCACGCTGTTCCTGCGGTACGTCTTCGATTTTAACCTGCTTGCCGTGCTCCTGGGTCGGCGCGTAAACCATGCGGATTTTTTTTCGACCCCATGGTGCGGCGCACGATa                                                                                                 >  1:536716/1‑133 (MQ=255)                           cttcGTTGGTCAGCGAGAAGATGTCACGCTGTTCCTGCGGTACGTCTTCGATTTTAACCTGCTTGCCGTGCTCCTGGGTCGGCGCGTAAACCATGCGGATTTTTTTTCGACCCCATGGTGCGGCGCACGATAg                                                                                                >  2:77114/1‑133 (MQ=255)                                   gTCAGCGAGAAGATGTCACGCTGTTCCTGCGGTACGTCTTCGATTTTAACCTGCTTGCCGTGCTCCTGGGTCGGCGCGTAAACCATGCGGATTTTTTTTCGACCCCATGGTGCGGCGCACGATAGCCGGGCGa                                                                                        >  2:25549/1‑133 (MQ=255)                                   gTCAGCGAGAAGATGTCACGCTGTTCCTGCGGTACGTCTTCGATTTTAACCTGCTTGCCGTGCTCCTGGGTCGGCGCGTAAACCATGCGGATTTTTTTTCGACCCCATGGTGCGGCGCACGATAGCCGGGCGa                                                                                        >  2:476976/1‑133 (MQ=255)                                     cAGCGAGAAGATGTCACGCTGTTCCTGCGGTACGTCTTCGATTTTAACCTGCTTGCCGTGCTCCTGGGTCGGCGCGTAAACCATGCGGATTTTTTTTCGACCCCATGGTGCGGCGCACGATAGCCGGGCGAtt                                                                                      >  1:564367/1‑133 (MQ=255)                                         gagaAGATGTCACGCTGTTCCTGCGGTACGTCTTCGATTTTAACCTGCTTGCCGTGCTCCTGGGTCGGCGCGTAAACCATGCGGATTTTTTTTCGACCCCATGGTGCGGCGCACGATAGCCGGGCGATTcgcc                                                                                  <  2:536716/133‑1 (MQ=255)                                           gaagaTGTCACGCTGTTCCTGCGGTACGTCTTCGATTTTAACCTGCTTGCCGTGCTCCTGGGTCGGCGCGTAAACCATGCGGATTTTTTTTCGACCCCATGGTGCGGCGCACGATAGCCGGGCGATTcgccgc                                                                                <  2:390751/133‑1 (MQ=255)                                           gaagaTGTCACGCTGTTCCTGCGGTACGTCTTCGATTTTAACCTGCTTGCCGTGCTCCTGGGTCGGCGCGTAAACCATGCGGATTTTTTTTCGACCCCATGGTGCGGCGCACGATAGCCGGGCGATTcgccgc                                                                                <  1:405205/133‑1 (MQ=255)                                             agaTGTCATGCTGTTTCTTCGGTACGTCTTTGATTTTAACCTGCTTGCCGTGCTCCTGGGTCGGCGCGTAAACCATGCGGATTTTTTTTCGACCCCATGGTGCGGCGCACGATAGCCGGGCGATTCGCCGCCa                                                                              <  1:184406/133‑1 (MQ=255)                                             agaTGTCACGCTGTTCCTGCGGTACGTCTTCGATTTTAACCTGCTTGCCGTGCTCCTGGGTCGGCGCGTAAACCATGCGGATTTTTTTTCGACCCCATGGTGCGGCGCACGATAGCCGGGCGATTCGCCGCCa                                                                              <  1:476976/133‑1 (MQ=255)                                                          ttCCTGCGGTACGTCTTCGATTTTAACCTGCTTGCCGTGCTCCTGGGTCGGCGCGTAAACCATGCGGATTTTTTTTCGACCCCATGGTGCGGCGCACGATAGCCGGGCGATTCGCCGCCAGTGTCGGTTTATg                                                                 >  2:356607/1‑133 (MQ=255)                                                                  gTACGTCTTCGATTTTAACCTGCTTGCCGTGCTCCTGGGTCGGCGCGTAAACCATGCGGATTTTTTTTCGACCCCATGGTGCGGCGCACGATAGCCGGGCGATTCGCCGCCAGTGTCGGTTTATGCACGTaaa                                                         >  1:539232/1‑133 (MQ=255)                                                                    aCGTCTTCGATTTTAACCTGCTTGCCGTGCTCCTGGGTCGGCGCGTAAACCATGCGGATTTTTTTTCGACCCCATGGTGCGGCGCACGATAGCCGGGCGATTTGCCGCCAGTGTGGGATTATGCCCGTAAAAc                                                       >  1:227336/1‑133 (MQ=255)                                                                          tCGATTTTAACCTGCTTGCCGTGCTCCTGGGTCGGCGCGTAAACCATGCGGATTTTTTTTCGACCCCATGGTGCGGCGCACGATAGCCGGGCGATTCGCCGCCAGTGTCGGTTTATGCACGTAAAACTCATcc                                                 >  2:106298/1‑133 (MQ=255)                                                                                      tGCTTGCCGTGCTCCTGGGTCGGCGCGTAAACCATGCGGATTTTTTTTCGACCCCATGGTGCGGCGCACGATAGCCGGGCGATTCGCCGCCAGTGTCGGTTTATGCACGTAAAACTCATCCGGGTTAACCGCa                                     <  2:380624/133‑1 (MQ=255)                                                                                      tGCTTGCCGTGCTCCTGGGTCGGCGCGTAAACCATGCGGATTTTTTTTCGACCCCATGGTGCGGCGCACGATAGCCGGGCGATTCGCCGCCAGTGTCGGTTTATGCACGTAAAACTCATCCGGGTTAACCGCa                                     <  2:564367/133‑1 (MQ=255)                                                                                                                gTAAACCATGCGGATTTTTTTTCGACCCCATGGTGCGGCGCACGATAGCCGGGCGATTCGCCGCCAGTGTCGGTTTATGCACGTAAAACTCATCCGGGTTAACCGCACCCTGCACGACCATCTCACCAAGGcc           <  1:106298/133‑1 (MQ=255)                                                                                                                 tAAACCATGCGGATTTTTTTTCGACCCCATGGTGCGGCGCACGATAGCCGGGCGATTCGCCGCCAGTGTCGGTTTATGCATGTAAAACTCATCCGGGTTAACCGCACCCTGCACGACCATCTCACCAAGGccc          >  2:342502/1‑133 (MQ=255)                                                                                                                         gCGGATTTTTTTTCGACCCCATGGTGCGGCGCACGATAGCCGGGCGATTCGCCGCCAGTGTCGGTTTATGCACGTAAAACTCATCCGGGTTAACCGCACCCTGCACGAACATCTCACCAAGGCCCCATGCGGa  >  2:267055/1‑133 (MQ=255)                                                                                                                              |                                                                                                                                GGCCTGTTTTGCCAGTTCCTGCACTTCTTCGTTGGTCAGCGAGAAGATGTCACGCTGTTCCTGCGGTACGTCTTCGATTTTAACCTGCTTGCCGTGCTCCTGGGTCGGCGCGTAAACCATGCGGA‑TTTTTTTCGACCCCATGGTGCGGCGCACGATAGCCGGGCGATTCGCCGCCAGTGTCGGTTTATGCACGTAAAACTCATCCGGGTTAACCGCACCCTGCACGACCATCTCACCAAGGCCCCATGCGGA  >  NC_000913/1786195‑1786446

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑