Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,702	A→G	D301G (GAT→GGT)	thrB →	homoserine kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,702	0	A	G	100.0%	61.8 / NA	18	D301G (GAT→GGT)	thrB	homoserine kinase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (6/12);  total (6/12)

TATCTCCGGCTCCGGCCCGACCTTGTTCGCTCTGTGTGACAAGCCGGAAACCGCCCAGCGCGTTGCCGACTGGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGCTGGATACGGCGGGCGCACGAGTACTGGAAAACTAAATGAAACTCTACAATCTGAAAGATCACAACGAGCAGGTCAGCTTTGCGCAAGCCGTAACCCAGGGGTT  >  NC_000913/3574‑3801                                                                                                                                 |                                                                                                    tATCTCCGGCTCCGGCCCGACCTTGTTCGCTCTGTGTGACAAGCCGGAAACCGCCCAGCGCGTTGCCGACTGGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGCTGGGTAcg                                                                                                 >  2:619393/1‑133 (MQ=255)       cggctccggcCCGACCTTGTTCGCTCTGTGTGACAAGCCGGAAACCGCCCAGCGCGTTGCCGACTGGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGCTGGGTACggcgggc                                                                                           >  1:602198/1‑133 (MQ=255)        ggctccggcCCGACCTTGTTCGCTCTGTGTGACAAGCCGGAAACCGCCCAGCGCGTTGCCGACTGGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGCTGGGTACggcgggcg                                                                                          >  1:505121/1‑133 (MQ=255)          ctccggcCCGTCCTTTTTCGCTCTCTGTGACATGCCGGAAAGCGCCCAGCGCGTTGCCGACTGGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGCTGGGTACGGCGGGCGCa                                                                                        <  2:207430/133‑1 (MQ=255)           tccggcCCGACCTTGGTCACTCTGTGTGACCAGCCGGAAACCGCCCAGCGCGTTGCCGACTGGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGCTGGGTATGGCGGGCGCAc                                                                                       <  1:262850/133‑1 (MQ=255)                    aCCTTGTTCGCTCTGTGTGACAAGCCGGAAACCGCCCAGCGCGTTGCCGACTGGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGCTGGGTACGGCGGGCGCACGAGTACTgg                                                                              >  1:151254/1‑133 (MQ=255)                     ccTTGTTCGCTCTGTGTGACAAGCCGGAAACCGCCCAGCGCGTTGCCGACTGGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGCTGGGTACGGCGGGCGCACGAGTACTGGa                                                                             <  1:568541/133‑1 (MQ=255)                             gCTCTGTGTGACAAGCCGGAAACCGCCCAGCGCGTTGCCGACTGGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGCTGGGTACGGCGGGCGCACGAGTACTGGAAAACTaaa                                                                     <  2:602198/133‑1 (MQ=255)                             gCTCTGTGTGACAAGCCGGAAACCGCCCAGCGCGTTGCCGACTGGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGCTGGGTACGGCGGGCGCACGAGTACTGGAAAACTaaa                                                                     <  2:151254/133‑1 (MQ=255)                               tctGTGTGACAAGCCGGAAACCGCCCAGCGCGTTGCCGACTGGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGCTGGGTACGGCGGGCGCACGAGTACTGGAAAACTAAATg                                                                   <  1:576615/133‑1 (MQ=255)                                 tgtgtgACAAGCCGGAAACCGCCCAGCGCGTTGCCGACTGGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGCTGGGTACGGCGGGCGCACGAGTACTGGAAAACTAAATGaa                                                                 <  2:319026/133‑1 (MQ=255)                                  gtgtgACAAGTCGGACACCGCCCATCGCGTTGACGACTGGTGGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGCTGGGTACGGCGGGCGCACGAGTACTGGAAAACTAAATGaaa                                                                <  2:173767/133‑1 (MQ=255)                                   tgtgACAAGCCGGAAACCGCCCAGCGCGTTGCCGACTGGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGCAGGGTACGGCGGGGGCCGGAGTAGTGGACAATCAAAAGaaaa                                                               >  2:284470/1‑132 (MQ=255)                                                      cccAGCGCGTTGCCGACTGGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGCTGGGTACGGCGGGCGCACGAGGACTGGGAAAATAAAAGAAAATCTACAAACTGAAAAAtaa                                            >  2:69802/1‑131 (MQ=255)                                                                                   aaCTTCCTACAAAATCAGAAAGCTTTTGTTCATATTTGCCGGCTGGGTACGGCGGGCGCACGAGTACTGGAAAACTAAATGAAACTCTACAATCTGAAAGATCACAACGAGCAGGTCAGCTTTGCGCAAGCCg               <  1:284470/133‑1 (MQ=255)                                                                                   aaCTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGCTGGGTACGGCGGGCGCACGAGTACTGGAAAACTAAATGAAACTCTACAATCTGAAAGATCACAACGAGCAGGTCAGCTTTGCGCAAGCCg               <  1:69802/133‑1 (MQ=255)                                                                                        ccTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGCTGGGTACGGCGGGCGCACCGGGACTGGAAAAATAAATGAAAAACTACAAACTGAAAGAACACAACGAGCAAGGCAGCTTTGCGCAAACCCTAAcc          >  2:102726/1‑133 (MQ=255)                                                                                                aTCAGGAAGGTTTTGTTCATATTTGCCGGCTGGGTACGGCGGGCGCACGAGTACTGGAAAACTAAATGAAACTCTACAATCTGAAAGATCACAACGAGCAGGTCAGCTTTGCGCAAGCCGTAACCCAGGGGtt  <  2:505121/133‑1 (MQ=255)                                                                                                aTCAGGAAGGTTTTGTTCATATTTGCCGGCTGGGTACGGCGGGCGCACGAGTACTGGAAAACTAAATGAAACTCTACAATCTGAAAGATCACAACGAGCAGGTCAGCTTTGCGCAAGCCGTAACCCAGGGGtt  <  1:513648/133‑1 (MQ=255)                                                                                                                                 |                                                                                                    TATCTCCGGCTCCGGCCCGACCTTGTTCGCTCTGTGTGACAAGCCGGAAACCGCCCAGCGCGTTGCCGACTGGTTGGGTAAGAACTACCTGCAAAATCAGGAAGGTTTTGTTCATATTTGCCGGCTGGATACGGCGGGCGCACGAGTACTGGAAAACTAAATGAAACTCTACAATCTGAAAGATCACAACGAGCAGGTCAGCTTTGCGCAAGCCGTAACCCAGGGGTT  >  NC_000913/3574‑3801

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑