Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,430,286	G→A	P160S (CCC→TCC)	cvpA ←	colicin V production protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,430,286	0	G	A	100.0%	79.9 / NA	26	P160S (CCC→TCC)	cvpA	colicin V production protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (16/10);  total (16/10)

CGTTAAGGCATCATAAATCGACTGGTTAACCGGCATAACACCGGCGATACCGACAATACCGCACATACGTCTTTTTCCTCGTTAAGCCACATCTCAGAGCACTTACGCTCTGGGCAAGAAACTTGACGAGCTTTGCAGATAATCAAAAAAACATCTGATGATAAAACTGAATTGCGGGATCAGCTGTGATTTGCTCCAGTCTTCGCTTTTCGACACCCCGGTAAAGGAGTCGAGA  >  NC_000913/2430173‑2430407                                                                                                                  |                                                                                                                          cGTTAAGGCATCATAAATCGACTGGTTAACCGTCATAACACCGGCGATACCGACAATACCGCACATACGTCTTTTTCCTCGTTAAGCCACATCTCAGAGCACTTACGCTCTGGACAAGAAACTTGACGAGCtt                                                                                                        >  2:40979/1‑133 (MQ=255)  gTTAAGGCATCATAAATCGACTGGTTAACCGGCATAACACCGGCGATACCGACAATACCGCACATACGTCTTTTTCCTCGTTAAGCCACATCTCAGAGCACTTACGCTCTGGACAAGAAACTTGACGAGCttt                                                                                                       >  1:320074/1‑133 (MQ=255)  gTTAAGGCATCATAAATCGACTGGTTAACCGGCATAACACCGGCGATACCGACAATACCGCACATACGTCTTTTTCCTCGTTAAGCCACATCTCAGAGCACTTACGCTCTGGACAAGAAACTTGACGAGCttt                                                                                                       >  2:472904/1‑133 (MQ=255)   ttAAGGCATCATAAATCGTCTGGTTAACCGGCATAACACCGGCGAGACCGACAATACCGCACATACGTCTTTTTCCTCGTTAAGCCACATCGCAGAGCACTTACCCGCTTGACAAGATACTTAGCGAGCttgg                                                                                                      >  2:311480/1‑131 (MQ=255)   ttAAGGCATCATAAATCGACTGGTTAACCGGCATAACACCGGCGATACCGACAATACCGCACATACGTCTTTTTCCTCGTTAAGCCACATCTCAGAGCACTTACGCTCTGGACAAGAAACTTGACGAGCTTTg                                                                                                      >  1:70338/1‑133 (MQ=255)         catcatAAATCGACTGGTTAACCGGCATAACACCGGCGATACCGACAATACCGCACATACGTCTTTTTCCTCGTTAAGCCACATCTCAGAGCACTTACGCTCTGGACAAGAAACTTGACGAGCTTTGCAGATa                                                                                                <  1:140535/133‑1 (MQ=255)           tcatAAATCGACTGGTTAACCGGCATAACACCGGCGATACCGACAATACCGCACATACGTCTTTTTCCTCGTTAAGCCACATCTCAGAGCACTTACGCTCTGGAGAAGAAACTTGACGAGCTTTGCAGATAAt                                                                                              >  2:362433/1‑133 (MQ=255)                  tCGACTGGTTAACCGGCATAACACCGGCGATACCGACAATACCGCACATACGTCTTTTTCCTCGTTAAGCCACATCTCAGAGCACTTACGCGCTGGACAATAAATTTGGCGAGCTTTGCAGATAATCaaaaaa                                                                                       >  2:255217/1‑133 (MQ=255)                        ggTTAACCGGCATAACACCGGCGATACCGACAATTCCGCACATACGTCTTGTTCCTCGTTAAGCCACATCTCAGAGCACTTACGCTCTGGACAAGAAACTTGACGAGCTTTGCAGATAATCAAAAAAACATCt                                                                                 <  2:382571/133‑1 (MQ=255)                         gTTAACCGGCATAACACCGGCGATACCGACAATACCGCACATACGTCTTTTTCCTCGTTAAGCCACATCTCAGAGCACTTACGCTCTGGACAAGAAACTTGACGAGCTTTGCAGATAATCAAAAAAACATCtg                                                                                >  2:14926/1‑133 (MQ=255)                                   aTAACACCGGCGATACCGACAATACCGCACATACGTCTTTTTCCTCGTTAAGCCACATCTCAGAGCACTTACGCTCTGGACAAGAAACTTGACGAGCTTTGCAGATAATCAAAAAAACATCTGATGATAAAAc                                                                      >  2:376945/1‑133 (MQ=255)                                           ggCGATACCGACAATACCGCACATACGTCTTTTTCCTCGTTAAGCCACATCTCAGAGCACTTACGCTCTGGACAAGAAACTTGACGAGCTTTGCAGATAATCAAAAAAACATCTGATGATAAAACTGAATTGc                                                              <  1:398406/133‑1 (MQ=255)                                                             gCACATACGTCTTTTTCCTCGTTAAGCCACATCTCAGAGCACTTACGCTCTGGACAAGAAACTTGACGAGCTTTGCAGATAATCAAAAAAACATCTGATGATAAAACTGAATTGCGGGATCAGCTGTGATTTg                                            >  1:94667/1‑133 (MQ=255)                                                                   aCGTCTTTTTCCTCGTTAAGCCACATCTCAGAGCACTTACGCTCTGGACAAGAAACTTGACGAGCTTTGCAGATAATCAAAAAAACATCTGATGATAAAACTGAATTGCGGGATCAGCTGTGATTTGCTCCAg                                      >  2:423773/1‑133 (MQ=255)                                                                       cTTTTTCCACGTTAAGCCGCATCTCAGAGCGCTTACGCTCTGGACAAGAAACTTGACGAGCTTTGCAGATAATCAAAAAAACATCTGATGATAAAACTGAATTGCGGGATCAGCTGTGATTTGCTCCAGTCtt                                  <  1:311480/133‑1 (MQ=255)                                                                           ttCCTCGTTAAGCCACATCTCAGAGCACTTACGCTCTGGACAAGAAACTTGACGAGCTTTGCAGATAATCAAAAAAACATCTGATGATAAAACTGAATTGCGGGATCAGCTGTGATTTGCTCCAGTCTTCGCt                              <  1:423773/133‑1 (MQ=255)                                                                            tCCTCGTTAAGCCACATCTCAGAGCACTTACGCTCTGGACAAGAAACTTGACGAGCTTTGCAGATAATCAAAAAAACATCTGATGATAAAACTGAATTGCGGGATCAGCTGTGATTTGCTCCAGTCTTCGCtt                             >  2:575094/1‑133 (MQ=255)                                                                                cGTTAAGCCACATCTCAGAGCACTTACGCTCTGGACAAGAAACTTGACGAGCTTTGCAGATAATCAAAAAAACATCTGATGATAAAACTGAATTGCGGGATCAGCTGTGATTTGCTCCAGTCTTCGCTTTTCg                         >  1:147902/1‑133 (MQ=255)                                                                                    aaGCCACATCTCAGAGCACTTACGCTCTGGACAAGAAACTTGACGAGCTTTGCAGATAATCAAAAAAACATCTGATGATAAAACTGAATTGCGGGATCAGCTGTGATTTGCTCCAGTCTTCGCTTTTCGacac                     <  2:70338/133‑1 (MQ=255)                                                                                    aaGCCACATCTCAGAGCACTTACGCTCTGGACAAGAAACTTGACGAGCTTTGCAGATAATCAAAAAAACATCTGATGATAAAACTGAATTGCGGGATCAGCTGTGATTTGCTCCAGTCTTCGCTTTTCGacac                     <  2:94667/133‑1 (MQ=255)                                                                                    aaGCCACATCTCAGAGCACTTACGCTCTGGACAAGAAACTTGACGAGCTTTGCAGATAATCAAAAAAACATCTGATGATAAAACTGAATTGCGGGATCAGCTGTGATTTGCTCCAGTCTTCGCTTTTCGacac                     <  1:472904/133‑1 (MQ=255)                                                                                           aTCTCAGAGCACTTACGCTCTGGACAAGAAACTTGACGAGCTTTGCAGATAATCAAAAAAACATCTGATGATAAAACTGAATTGCGGGATCAGCTGTGATTTGCTCCAGTCTTCGCTTTTCGACACCCCGGTa              >  2:504260/1‑133 (MQ=255)                                                                                                 gagCACTTACGCTCTGGACAAGAAACTTGACGAGCTTTGCAGATAATCAAAAAAACATCTGATGATAAAACTGAATTGCGGGATCAGCTGTGATTTGCTCCAGTATACGCTTTTCGACACCCCGGTAAAGGAg        >  2:303760/1‑133 (MQ=255)                                                                                                     aCTTACGCTCTGGACAAGAAACTTGACGAGCTTTGCAGATAATCAAAAAAACATCTGATGATAAAACTGAATTGCGGGATCAGCTGTGATTTGCTCCAGTCTTCGCTTTTCGACACCCCGGTAAAGGAGTCga    <  1:362433/133‑1 (MQ=255)                                                                                                       ttACGCTCTGGACAAGAAACTTGACGAGCTTTGCAGATAATCAAAAAAACATCTGATGATAAAACTGAATTGCGGGATCAGCTGTGATTTGCTCCAGTCTTCGCTTTTCGACACCCCGGTAAAGGAGTCgaga  >  2:91701/1‑133 (MQ=255)                                                                                                       ttACGCTCTGGACAAGAAACTTGACGAGCTTTGCAGATAATCAAAAAAACATCTGATGATAAAACTGAATTGCGGGATCAGCTGTGATTTGCTCCAGTCTTCGCTTTTCGACACCCCGGTAAAGGAGTCgaga  <  1:14926/133‑1 (MQ=255)                                                                                                                  |                                                                                                                          CGTTAAGGCATCATAAATCGACTGGTTAACCGGCATAACACCGGCGATACCGACAATACCGCACATACGTCTTTTTCCTCGTTAAGCCACATCTCAGAGCACTTACGCTCTGGGCAAGAAACTTGACGAGCTTTGCAGATAATCAAAAAAACATCTGATGATAAAACTGAATTGCGGGATCAGCTGTGATTTGCTCCAGTCTTCGCTTTTCGACACCCCGGTAAAGGAGTCGAGA  >  NC_000913/2430173‑2430407

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑