Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,533,649	G→A	intergenic (+269/‑115)	cysK → / → ptsH	O‑acetylserine sulfhydrylase A/phosphocarrier protein HPr

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,533,649	0	G	A	100.0%	63.4 / NA	21	intergenic (+269/‑115)	cysK/ptsH	O‑acetylserine sulfhydrylase A/phosphocarrier protein HPr
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (8/13);  total (8/13)

CAGGAAAAGCCAAAGCTGAATCGATTTTATGATTTGGTTCAATTCTTCCTTTAGCGGCATAATGTTTAATGACGTACGAAACGTCAGCGGTCAACACCCGCCAGCAATGGACTGTATTGCGCTCTTCGTGCGTCGCGTCTGTTAAAAACTGGCGCTAACAATACAGGCTAAAGTCGAACCGCCAGGCTAGACTTTAGTTCCACAACACTAAACCTATAAGTTGGGGAAATACAATGTTCCAGCA  >  NC_000913/2533531‑2533774                                                                                                                       |                                                                                                                              cAGGAAAAGCCAAAGCTGAATCGATTTTATGATTTGGTTCAATTCTTCCTTTAGCGGCATAATGTTTAATGACGTACGAAACGTCAGCGGTCAACACCCGCCAGCAATGGACTGTATTACGCTCTTCGTgcgt                                                                                                                 >  1:90606/1‑133 (MQ=255)      aaaGCCAAAGCTGAATCGATTTTATGATTTGGTTCAATTCTTCCTTTAGCGGCATAATGTTTAATGACGTACGAAACGTCAGCGGTCAACACCCGCCAGCAATGGACTGTATTACGCTCTTCGTgcgtcgcgt                                                                                                            <  2:628799/133‑1 (MQ=255)            aaaGCTGAATCGATTTTATGATTTGGTTCAATTCTTCCTTTAGCGGCATAATGTTTAATGACGTACGAAACGTCAGCGGTCAACACCCGCCAGCAATGGACTGTATTACGCTCTTCGTGCGTCGCGTCTGTTa                                                                                                      >  1:428410/1‑133 (MQ=255)                     tCGATTTTATGATTTGGTTCAATTCTTCCTTTAGCGGCATAATGTTTAATGACGTACGAAACGTCAGCGGTCAACACCCGCCAGCAATGGACTGTATTACGCTCTTCGTGCGTCGCGTCTGTTAAAAACTGgc                                                                                             <  2:450930/133‑1 (MQ=255)                     tCGATTTTATGATTTGGTTAAATTCTTCCTTTAGCGGCATAATTTTTAATGACGTACGAACCGTCAGCGGTCAACACCCGCCAGCAATGGACTGTATTACGCTCTTCGTGCGTCGCGTCTGTTAAAAACTGgc                                                                                             <  2:580742/133‑1 (MQ=255)                          tttATGATTTGGTTCAATTCTTCCTTTAGCGGCATAATGTTTAATGACGTACGAAACGTCAGCGGTCAACACCCGCCAGCAATGGACTGTATTACGCTCTTCGTGCGTCGCGTCTGTTAAAAACTGGCGCTaa                                                                                        <  2:428410/133‑1 (MQ=255)                                                ccTTTAGCGGCATAATGTTTAATGACGTACGAAACGTCAGCGGTCAACACCCGCCAGCAATGGACTGTATTACGCTCTTCGTGCGTCGCGTCTGTTAAAAACTGGCGCTAACAATACAGGCTAAAGTCGAAcc                                                                  >  2:547025/1‑133 (MQ=255)                                                   ttAGCGGCATAATTTTTAATGACGTACGAACCGTCAGGGGTCAACACCCGCCAGCAATGGACTGTATTACGCTCTTCGTGCGTCGCGTCTGTTAAAAACTGGCGCTAACAATACAGGCTAAAGTCGAACCGcc                                                               <  2:105377/133‑1 (MQ=255)                                                     aGCGGCATAATGTTTAATGACGTACGAAACGTCAGCGGTCAACACCCGCCAGCAATGGACTGTATTACGCTCTTCGTGCGTCGCGTCTGTTAAAAACTGGCGATAACAATACAGCCTAAAGTCGAACCGCCAg                                                             >  1:243240/1‑133 (MQ=255)                                                          cATAATGTTTAATGACGTACGAAACGTCAGCGGTCAACACCCGCCAGCAATGGACTGTATTACGCTCTTCGTGCGTCGCGTCTGTTAAAAACTGGCGCTAACAATACAGGCTAAAGTCGAACCGCCAGGCTAg                                                        >  1:105377/1‑133 (MQ=255)                                                          cATAATGTTTAATGACGTACGAAACGTCAGCGGTCAACACCCGCCAGCAATGGACTGTATTACGCTCTTCGTGCGTCGCGTCTGTTAAAAACTGGCGCTAACAATACAGGCTAAAGTCGAACCGCCAGGCTAg                                                        >  2:444834/1‑133 (MQ=255)                                                                 tttAATGACGTACAAACCGTCGGCGGCCAACACCCGCCAGCAATGGACTGTATTACGCTCTTCGTGCGTCGCGTCTGTTAAAAACTGGCGCTAACAATACAGGCTAAAGTCGAACCGCCAGGCTAGACTTTAg                                                 <  2:90606/133‑1 (MQ=255)                                                                 ttaaaTGACGGACAACACGTCAGCTGTATACCCCCGCCAGCCATGGACTGTATTACGCTCTTCGTGCGTCGCGTCTGTTAAAAACTGGCGCTAACAATACAGGCTAAAGTCGAACCGCCAGGCTAGACTTTAg                                                 <  2:170952/130‑1 (MQ=255)                                                                         cGTACGAAACGTCAGCGGTCAACACCCGCCAGCAATGGACTGTATTACGCTCTTCGTGCGTCGCGTCTGTTAAAAACTGGCGCTAACAATACAGGCTAAAGTGGAACCGCCAGGCTAGACTTTAGTTCCacaa                                         >  1:170952/1‑133 (MQ=255)                                                                         cGTACGAAACGTCAGCGGTCAACACCCGCCAGCAATGGACTGTATTACGCTCTTCGTGCGTCGCGTCTGTTAAAAACTGGCGCTAACAATACAGGCTAAAGTCGAACCGCCAGGCTAGACTTTAGTTCCacaa                                         >  2:4998/1‑133 (MQ=255)                                                                                         ggcacacacCCGCCAGCAATGGACTGTATTACGCTCTTCGTGCGTCGCGTCTGTTAAAAACTGGCGCTAACAATACAGGCTAAAGTCGAACCGCCAGGCTAGACTTTAGTTCCACAACACTAAACCTATAAGt                         <  2:562647/128‑1 (MQ=255)                                                                                           tCAACACCCGCCAGCAATGGACTGTATTACGCTCTTCGTGCGTCGCGTCTGTTAAAAACTGGCGCTAACAATACAGGCTAAAGTCGAACCGCCAGGCTAGACTTTAGTTCCACAACACTAAACCTATAAGTTg                       <  1:4998/133‑1 (MQ=255)                                                                                                               aCTGTATTACGCTCTTCGTGCGTCGCGTCTGTTAAAAACTGGCGCTAACAATACAGGCTAAAGTCGAACCGCCAGGCTAGACTTTAGTTCCACAACACTAAACCTATAAGTTGGGGAAATACAATGTTCCAGc   <  2:331571/133‑1 (MQ=255)                                                                                                               aCTGTATTACGCTCTTCGTGCGTCGCGTCTGTTAAAAACTGGCGCTAACAATACAGGCTAAAGTCGAACCGCCAGGCTAGACTTTAGTTCCACAACACTAAACCTATAAGTTGGGGAAATACAATGTTCCAGc   <  1:444834/133‑1 (MQ=255)                                                                                                                cTGTATTACGCTCTTCGTGCGTCGCGTCTGTTAAAAACTGGCGCTAACAATACAGGCTAAAGTCGAACCGCCAGGCTAGACTTTAGTTCCACAACACTAAACCTATAAGTTGGGGAAATACAATGTTCCAGCa  <  1:613661/133‑1 (MQ=255)                                                                                                                cTGTATTACGATCTTGGTGCGTCGTGTCCGTTTAAAACTGGTGCTAACAATACAGGCTAAAGTCGAACCGCCAGGCTAGACTTTAGTTCCACAACACTAAACCTATAAGTTGGGGAAATACAATGTTCCAGCa  <  2:243240/133‑1 (MQ=255)                                                                                                                       |                                                                                                                              CAGGAAAAGCCAAAGCTGAATCGATTTTATGATTTGGTTCAATTCTTCCTTTAGCGGCATAATGTTTAATGACGTACGAAACGTCAGCGGTCAACACCCGCCAGCAATGGACTGTATTGCGCTCTTCGTGCGTCGCGTCTGTTAAAAACTGGCGCTAACAATACAGGCTAAAGTCGAACCGCCAGGCTAGACTTTAGTTCCACAACACTAAACCTATAAGTTGGGGAAATACAATGTTCCAGCA  >  NC_000913/2533531‑2533774

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑