Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,609,780	G→A	E432K (GAA→AAA)	hyfG →	hydrogenase 4 catalytic subunit HyfG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,609,780	0	G	A	100.0%	68.8 / NA	22	E432K (GAA→AAA)	hyfG	hydrogenase 4 catalytic subunit HyfG
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (13/9);  total (13/9)

CTTTGATCACCCCTACGCCGACTACGGCAATATTCCAAAAACACTGTTTACCTTTACCGGCGGCGATGTTTTCTCCCGCGTGATGGTCCGTGTCAAAGAGACGTTTGATTCGCTGGCAATGCTGGAATTTGCCCTCGACAACATGCCGGATACCCCACTGCTGACCGAAGGCTTTAGCTATAAACCTCACGCATTCGCGCTGGGCTTTGTTGAAGCGCCACGCGGTGAAGACGTGCACT  >  NC_000913/2609656‑2609894                                                                                                                             |                                                                                                                   cTTTGATCACCCCTACGCCGACTACGGCAATATTCCAAAAACACTGTTTACCTTTACCGGCGGCGATGTTTTCTCCCGCGTGATGGTCCGTGTCAAAGAGACGTTTGATTCGCTGGCAATGCTGAAATTTGcc                                                                                                            <  1:619465/133‑1 (MQ=255)             cTACGCCGACTACGGCAATATTCCAAAAACACTGTTTACCTTTACCGGCGGCGATGTTTTCTCCCGCGTGATGGTCCGTGTCAAAGAGACGTTTGATTCGCTGGCAATGCTGAAATTTGCCCTCGACAACATg                                                                                                <  1:151078/133‑1 (MQ=255)              tACGCCGACTACGGCAATATTCCAAAAACACTGTTTACCTTTACCGGCGGCGATGTTTTCTCCCGCGTGATGGTCCGTGTCAAAGAGACGTTTGATTCGCTGGCAATGCTGAAATTTGCCCTCGACAACATGc                                                                                               >  2:430723/1‑133 (MQ=255)                cGCCGACTACGGCAATATTCCAAAAACACTGTTTACCTTTACCGGCGGCGATGTTTTCTCCCGCGTGATGGTCCGTGTCAAAGAGACGTTTGATTCGCTGGCAATGCTGAAATTTGCCCTCGACAACATGCCg                                                                                             >  1:115336/1‑133 (MQ=255)                  ccGACTACGGCAATATTCCAAAAACACTGTTTACCTTTACCGGCGGCGATGTTTTCTCCCGCGTGATGGTCCGTGTCAAAGAGACGTTTGATTCGCTGGCAATGCTGAAATTTGCCCTCGACAACATGCCGGa                                                                                           <  1:456637/133‑1 (MQ=255)                     aCTACGGCAATATTCCAAAAACACTGTTTACCTTTACCGGCGGCGATGTTTTCTCCCGCGTGATGGTCCGTGTCAAAGAGACGTTTGATTCGCTGGCAATGCTGAAATTTGCCCTCGCCAACATCCCGGATAc                                                                                        >  1:234280/1‑133 (MQ=255)                     aCTACGGCAATATTCCAAAAACACTGTTTACCTTTACCGGCGGCGATGTTTTCTCCCGCGTGATGGTCCGTGTCAAAGAGACGTTTGATTCGCTGGCAATGCTGAAATTTGCCCTCGACAACATGCCGGAt                                                                                          >  2:429985/1‑131 (MQ=255)                     aCTACGGCAATATTCCAAAAACACTGTTTACCTTTACCGGCGGCGATGTTTTCTCCCGCGTGATGGTCCGTGTCAAAGAGACGTTTGATTCGCTGGCAATGCTGAAATTTGCCCTCGACAACATGCCGGAt                                                                                          <  1:429985/131‑1 (MQ=255)                     aCTACGGCAATATTCCAAAAACACTGTTTACCTTTACCGGCGGCGATGTTTTCTCCCGCGTGATGGTCCGTGTCAAAGAGACGTTTGATTCGCTGGCAATGCTGAAATTTACCCCCGCCAACATGCCGGATAc                                                                                        >  1:314522/1‑133 (MQ=255)                       tACGGCAATATTCCAAAAACACTGTTTACCTTTACCGGCGGCGATGTTTTCTCCCGCGTGATGGTCCGTGTCAAAGAGACGTTTGATCCGCTGGCAATGCTGAAATTTGCCCTCGACAACATGCCGGATAccc                                                                                      <  2:388188/133‑1 (MQ=255)                                            cTGTTTACCTTTACCGGCGGCGATGTTTTCTCCCGCGTGATGGTCCGTGTCAAAGAGACGTTTGATTCGCTGGCAATGCTGAAATTTGCCCGCGACAACATGCCAGATGCCCCACTGTTGACAGAAGGCTTTa                                                                 >  2:250416/1‑133 (MQ=255)                                                       taccGGCGGCGATGTTTTCTCCCGCGTGATGGTCCGTGTCAAAGAGACGTTTGATTCGCTGGCAATGCTGAAATTTGCCCTCGACAACATGCCGGATACCCCTCTGCTGAACGACGGATTTAGCGATAAACCt                                                      >  1:295256/1‑133 (MQ=255)                                                       taccGGCGGCGATGTTTTCTCCCGCGTGATGGTCCGTGTCAAAGAGACGTTTGATTCGCTGGCAATGCTGAAATTTGCCCTCGACAACATGCCGGATACCCCACTGCTGACCGAAGGCTTTAGCTATAAACCt                                                      >  1:478452/1‑133 (MQ=255)                                                                   ttttttCTCCCGCGTCATGGTCCATGCCTAAGTGACGTTTGTTTCGCTGGCAATGCTGAAATTTGCCCTCGACAACATGCCGGATACCCCACTGCTGACCGAAGGCTTTAGCTATAAACCTCACGCATTcgcg                                          <  2:295256/131‑1 (MQ=255)                                                                                gTGATGGTCCTTGTCAAAGAGACGTTTGCTTCCCTGGCAATGCTGAAATTTGCCCTCGACAACATGCCGGATACCCCACTGCTGACCGAAGGCTTTAGCTATAAACCTCACGCATTCGCGCTGGGCTttgttg                             <  2:234280/133‑1 (MQ=255)                                                                                  gATGGTCCGTGTCAAAGAGACGTTTGATTCGCTGGCAATGCTGAAATTTGCCCTCGACAACATGCCGGATACCCCACTGCTGACCGAAGGCTTTAGCTATAAACCTCACGCATTCGCGCTGGGCTTTGTTGaa                           <  2:478452/133‑1 (MQ=255)                                                                                                aaGAGACGTTTGATTCGCTGGCAATGCTGAAATTTGCCCTCGACAACATGCCGGATACCCCACTGCTGACCGAAGGCTTTAGCTATAAACCTCACGCATTAGCGCTGGGCTTTGTTGAAGCGACACGCGGTGa             >  2:254760/1‑133 (MQ=255)                                                                                                     aCGTTTGATTCGCTGGCAATGCTGAAATTTGCCCTCGACAACATGCCGGATACCCCACTGCTGACCGAAGGCTTTAGCTATAAACCTCACGCATTCGCGCTGAGCTTTGTAGAAACGCCACGCGGTGAAGACg        >  1:207198/1‑133 (MQ=255)                                                                                                      cGTTTGATTCGCTGGCAATGCTGAAATTTGCCCTCGACAACATGCCGGATACCCCACTGCTGACCGAAGGCTTTAGCTATAAACCTCACGCATTCGCGCTGGGCTTTGTTGAAGCGCCACGCGGTGAAGACGt       >  1:94647/1‑133 (MQ=255)                                                                                                       gTTTGATTCGCTGGCAATGCTGAAATTTGCCCTCGACAACATGCCGGATACCCCACTGCTGACCGAAGGCTTTAGCTATAAACCTCACGCATTCGCGCTGGGCTTTGTTGAAGCGCCACGCGGTGAAGACGTg      >  1:615181/1‑133 (MQ=255)                                                                                                          tGATTCGCTGGCAATGCTGAAATTTGCCCTCGACAACATGCCGGATACCCCACTGCTGACCGAAGGCTTTAGCTATAAACCTCACGCATTCGCGCTGGGCTTTGTTGAAGCGCCACGCGGTGAAGACGTGCAc   <  2:115336/133‑1 (MQ=255)                                                                                                           gATTCGCTGGCAATGCTGAAATTTGCCCTCGACAACATGCCGGATACCCCACTGCTGACCGAAGGCTTTAGCTATAAACCTCACGCATTCGCGCTGGGCTTTGTTGAAGCGCCACGCGGTGAAGACGTGCACt  >  2:633208/1‑133 (MQ=255)                                                                                                                             |                                                                                                                   CTTTGATCACCCCTACGCCGACTACGGCAATATTCCAAAAACACTGTTTACCTTTACCGGCGGCGATGTTTTCTCCCGCGTGATGGTCCGTGTCAAAGAGACGTTTGATTCGCTGGCAATGCTGGAATTTGCCCTCGACAACATGCCGGATACCCCACTGCTGACCGAAGGCTTTAGCTATAAACCTCACGCATTCGCGCTGGGCTTTGTTGAAGCGCCACGCGGTGAAGACGTGCACT  >  NC_000913/2609656‑2609894

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑