Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,678,478	T→C	D297G (GAC→GGC)	yphF ←	putative ABC transporter periplasmic binding protein YphF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,678,478	0	T	C	100.0%	113.1 / NA	33	D297G (GAC→GGC)	yphF	putative ABC transporter periplasmic binding protein YphF
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (20/13);  total (20/13)

TGCCGTGAACATAGGGACCTCTGCGAATCAGCGATTAGGGCAGACCATCAACGTGCGTTGCCAGCCACTGTTTACCGTCTTCCGTTTTGGTATAGAGATCGATAGGCACCTGAATCACTTTTTCACCGTCGGCTTGTTTATTGATAACCTTCAATGTTTGCGCGAAAACAGCATTGCCCATTTTCTTACCGGAAATATCCACTACCGCTTTCAGCACCTGATTGTTTTCCAGCTCCTGAGCAATTTCGGTTGTCA  >  NC_000913/2678350‑2678604                                                                                                                                 |                                                                                                                               tGCCGTGAACATAGGGACCTCTGCGAATCAGCGATTAGGGCAGACCATCAACGTGCGTTGCCAGCCACTGTTTACCGTCTTCCGTTTTGGTATAGAGATCGATAGGCACCTGAATCACTTTTTCACCGCCGGc                                                                                                                            <  1:99788/133‑1 (MQ=255)        aaCATAGGTACCTCTCCGTATCTGCGATTAGGGCAGACCATCAACGTGCGTTGCCAGCCACTGTTTACCGTCTTCCGTTTTGGTATAGAGATCGATAGGCACCTGAATCACTTTTTCACCGCCGGCTTGTTTa                                                                                                                     <  1:269822/133‑1 (MQ=255)        aaCATAGGGACCTCTGCGAATCAGCGATTAGGGCAGACCATCAACGTGCGTTGCCAGCCACTGTTTACCGTCTTCCGTTTTGGTATAGAGATCGATAGGCACCTGAATCACTTTTTCACCGCCGGCTTGTTTa                                                                                                                     >  1:489017/1‑133 (MQ=255)             aGGGACCTCTGCGAATCAGCGATTAGGGCAGACCATCAACGTGCGTTGCCAGCCACTGTTTACCGTCTTCCGTTTTGGTATAGAGATCGATAGGCACCTGAATCCCTTTTTCACCGCCGGCTTGTTTATTGAt                                                                                                                <  1:127527/133‑1 (MQ=255)             aGGGACCTCTGCGAATCAGCGATTAGGGCAGACCATCAACGTGCGTTGCCAGCCACTGTTTACCGTCTTCCGTTTTGGTATAGAGATCGATAGGCACCTGAATCACTTTTTCACCGCCGGCTTGTTTATTGa                                                                                                                 >  1:588299/1‑132 (MQ=255)             aGGGACCTCTGCGAATCAGCGATTAGGGCAGACCATCAACGTGCGTTGCCAGCCACTGTTTACCGTCTTCCGTTTTGGTATAGAGATCGATAGGCACCTGAATCACTTTTTCACCGCCGGCTTGTTTATTGa                                                                                                                 <  2:588299/132‑1 (MQ=255)             aGGGACCTCTGCGAATCAGCGATTAGGGCAGACCATCAACGTGCGTTGCCAGCCACTGTTTACCGTCTTCCGTTTTGGTATAGAGATCGATAGGCACCTGAATCACTTTTTCACCGCCGGCTTGTTTATTGAt                                                                                                                <  1:613355/133‑1 (MQ=255)             aGGGACCTCTGCGAATCAGCGATTAGGGCAGACCATCAACGTGCGTTGCCAGCCACTGTTTACCGTCTTCCGTTTTGGTATAGAGATCGATAGGCACCTGAATCACTTTTCCACCGCAGGCTTGATTATTGAt                                                                                                                >  1:214613/1‑133 (MQ=255)                  ccTCTGCGAATCAGCGATTAGGGCAGACCATCAACGTGCGTTGCCAGCCACTGTTTACCGTCTTCCGTTTTGGTATAGAGATCGATAGGCACCTGAATCACTTTTTCACCGCCGGCTTGTTTATTGATAACCt                                                                                                           >  2:602687/1‑133 (MQ=255)                   ctctGCGAATCAGCGATTAGGGCAGACCATCAACGTGCGTTGCCAGCCACTGTTTACCGTCTTCCGTTTTGGTATAGAGATCGATAGGCACATGAATCACATTTTCACCGCCGGCTTGTTTAATGATAACCtt                                                                                                          >  2:279573/1‑133 (MQ=255)                     ctGCGAATCAGCGATTAGGGCAGACCATCAACGTGCGTTGCCAGCCACTGTTTACCGTCTTCCGTTTTGGTATAGAGATCGATAGGCACCTGAATCACTTTTTCACCGCCGGCTTGTTTATTGATAACCTTCa                                                                                                        >  2:346482/1‑133 (MQ=255)                         gAATCAGCGATTAGGGCAGACCATCAACGTGCGTTGCCAGCCACTGTTTACCGTCTTCCGTTTTGGTATAGAGATCGATAGGCACCTGAATCACTTTTTCACCGCCGGCTTGTTTATTGATAACCTTCAATGt                                                                                                    >  1:39212/1‑133 (MQ=255)                                cGATTGGGGCAGACCATCAACGTGCGTTGCCAGCCACTGTTTACCGTCTTCCGTTTTGGTATAGAGATCGATAGGCACCTGAATCACTTTTCCACCGCCGCCTTGTTTCTTGATAACGTTCAGTGTTTGcgcg                                                                                             >  2:235835/1‑133 (MQ=255)                                    tAGGGCAGACCATCAACGTGCGTTGCCAGCCACTGTTTACCGTCTTCCGTTTTGGTATAGAGATCGATAGGCACCTGAATCACTTTTTCACCGCCGGCTTGTTTATTGATAACCTTCAATGTTTGCGCGaaaa                                                                                         <  1:602687/133‑1 (MQ=255)                                     aGGGCAGACCATCAACGTGCGTTGCCAGCCACTGTTTACCGTCTTCCGTTTTGGTATAGAGATCGATAGGCACCTGAATCACTTTTTCACCGCCGGCTTGTTAATTGATAACCTGCAGTGTTTGAGCGAAAAc                                                                                        >  1:296431/1‑133 (MQ=255)                                          aGACCATCAACGTGCGTTGCCATCCACTGTTTACCGTCTTCCGTTTTGGTATAGAGATCGATAGGCACCTGAATCACTTTTTCACCGCCGGCTTGTTTATTGATAACCTTCAATGTTTGCGCGAAAACAGCAt                                                                                   <  1:279573/133‑1 (MQ=255)                                                    cGTGCGTTGCCAGCCACTGTTTACCGTCTTCCGTTTTGGTATAGAGATCGATAGGCACCTGAATCACTTTTTCACCGCCGGCTTGTTTATTGATAACCTTCAATGTTTGCGCGAAAACAGCATTGCCCAtttt                                                                         >  1:629217/1‑133 (MQ=255)                                                        cGTTGCCAGCCACTGTTTACCGTCTTCCGTTTTGGTATAGAGATCGATAGGCACCTGAATCACTTTTTCACCGCCGGCTTGTTTATTGATAACCTTCAATGTCTGCGCGAAAACGGCATTGCCCTTTTTCTTa                                                                     >  1:208759/1‑133 (MQ=255)                                                                gccaCTGTTTACCGTCTTCCGTTTTGGTATAGAGATCGATAGGCACCTGAATCACTTTTTCACCGCCGGCTTGTTTATTGATAACCTTCAATGTTTGCGCGAAAACAGCATTGCCCATTTTCTTACCGGAAat                                                             <  2:629217/133‑1 (MQ=255)                                                                     tGTTTACCGTCTTCCGTTTTGGTATAGAGATCGATAGGCACCTGAATCACTTTTTCACCGCCGGCTTGTTTATTGATAACCTTCAATGTTTGCGCGAAAACAGCATTGCCCATTTTCTTACCGGAAATATCCa                                                        >  2:93621/1‑133 (MQ=255)                                                                       tttACCGTCTTCCGTTTTGGTATAGAGATCGATAGGCACCTGAATCACTTTTTCACCGCCGGCTTGTTTATTGATAACCTTCAATGTTTGCGCGAAAACAGCATTGCCCATTTTCTTACCGGAAATATCCACt                                                      >  2:586131/1‑133 (MQ=255)                                                                                ttCCGTTTTGGTATAGAGATCGATAGGCACCTGAATCACTTTTTCACCGCCGGCTTGTTTATTGATAACCTTCAATGTTTGCGCGAAAACAGCATTGCCCCTTTACTTGCCGGAAATATCCACTACCGCTTTc                                             >  2:238031/1‑133 (MQ=255)                                                                                 tCCGTTTTGGTATAGAGATCGATAGGCACCTGAATCACTTTTTCACCGCCGGCTTGTTTATTGATAACCTTCAATGTTTGCGCGAAAACAGCATTGCCCATTTTCTTACCGGAAATATCCACTACCGCTTTCa                                            <  1:346482/133‑1 (MQ=255)                                                                                 tCCGTTTTGGTATAGAGATCGATAGGCACCTGAATCACTTTTTCACCGCCGGCTTGTTTATTGATAACCTTCAATGTTTGCGCGAAAACAGCATTGCCCATTATCTTACCGGAACTACCCACTACCGCTTTCa                                            >  1:260742/1‑133 (MQ=255)                                                                                            ataGAGATCGATAGGCACCTGAATCACTTTTTCACCGCCGGCTTGTTTATTGATAACCTTCAATGTTTGCGCGAAAACAGCATTGCCCATTTTCTTACCGGAAATATCCACTACCGCTTTCAGCACCTGATTg                                 <  1:586131/133‑1 (MQ=255)                                                                                                   tCGATAGGCACCTGAATCACTTTTTCACCGCCGGCTTGTTTATTGATAACCTTCAATGTTTGCGCGAAAACAGCATTGCCCATTTTCTTACCGGAAATATCCACTACCGCTTTCAGCACCTGATTGTTTTCCa                          >  2:151895/1‑133 (MQ=255)                                                                                                    cGATAGGCACCTGAATCACTTTTTCACCGCCGGCTTGTTTATTGATAACCTTCAATGTTTGCGCGAAAACAGCATTGCCCATTTTCTTACCGGAAATATCCAATACCGCTTTCATCAACTGATTGTTTTCCAg                         >  1:291516/1‑133 (MQ=255)                                                                                                    cGATAGGCACCTGAATCACTTTTTCACCGCCGGCTTGTTTATTGATAACCTTCAATGTTTGCGCGAAAACAGCATTGCCCATTTTCTTACCCGAAATATCTACTCCCGATTTCAGCATCTGATTGTTTTCCAg                         >  2:231168/1‑133 (MQ=255)                                                                                                       tAGGCACCTGAATCACTTTTTCACCGCCGGCTTGTTTATTGATAACCTTCAATGTTTGCGCGAAAACAGCATTGCCCATTTTCTTACCGGAAATATCCACTACCGCTTTCAGCACCTGATTGTTTTCCAGCTc                      <  2:39212/133‑1 (MQ=255)                                                                                                                    cccTTTTTCGACGCCGGCTTGTTTATTGTTAACCTTCAATGTTTGCGCGAAAACAGCATTGCCCATTTTCTTACCGGAAATATCCACTACCGCTTTCAGCACCTGATTGTTTTCCAGCTCCTGAGCAATTTCg         <  2:291516/131‑1 (MQ=255)                                                                                                                     attttttCAGCGCCGTCTTGTTTGTTGAGAACCTTCAATGTTTGCGCGAAAACAGCATTGCCCATTTTCTTACCGGAAATATCCACTACCGCTTTCAGCACCTGATTGTTTTCCAGCTCCTGAGCAATTTCgg        <  2:296431/131‑1 (MQ=255)                                                                                                                        ttttCACCGCCGGCTTGTTTATTGATAACCTTCAATGTTTGCGCGAAAACAGCATTGCCCATTTTCTTACCGGAAATATCCACTACCGCTTTCAGCACCTGATTGTTTTCCAGCTCCTGAGCAATTTCGGTTg     >  2:369764/1‑133 (MQ=255)                                                                                                                           tCACCGCCGGCTTGTTTATTGATAACCTTCAATGTTTGCGCGAAAACAGCATTGCCCATTTTCTTACCGGAAATATCCACTACCGCTTTCAGCACCTGATTGATTTCCAGATCCGGAGCAATTTTGGTTGTCa  >  1:175905/1‑133 (MQ=255)                                                                                                                                 |                                                                                                                               TGCCGTGAACATAGGGACCTCTGCGAATCAGCGATTAGGGCAGACCATCAACGTGCGTTGCCAGCCACTGTTTACCGTCTTCCGTTTTGGTATAGAGATCGATAGGCACCTGAATCACTTTTTCACCGTCGGCTTGTTTATTGATAACCTTCAATGTTTGCGCGAAAACAGCATTGCCCATTTTCTTACCGGAAATATCCACTACCGCTTTCAGCACCTGATTGTTTTCCAGCTCCTGAGCAATTTCGGTTGTCA  >  NC_000913/2678350‑2678604

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑