Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,682,164	T→C	A194A (GCA→GCG)	yphG ←	DUF5107 domain‑containing protein YphG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,682,164	0	T	C	100.0%	112.1 / NA	32	A194A (GCA→GCG)	yphG	DUF5107 domain‑containing protein YphG
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (18/14);  total (18/14)

TTTGTAGTAAGTGCCGGTAGCGATGGGGAAAGCGGAGACGGCCCGTTTGCCGTGATCAAACACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTTCCCCCCCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAGGGCGCAGGGTGAAACCTGTCATCACCTGTAAACCATGCATCGGC  >  NC_000913/2682050‑2682293                                                                                                                   |                                                                                                                                  tttGTAGTAAGTGCCGGTAGCGATGGGGAAAGCGGAGACGGCCCGTTTGCCGTGATCAAACACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTTCCCCCCCTTTCACCGCCGGGCTGGCCcaccac                                                                                                                 >  1:390123/1‑133 (MQ=255)   tGTAGTAAGTGCCGGTAGCGATGGGGAAAGCGGAGACGGCCCGTTTGCCGTGATCAAACACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTTCCCCCCCTTTCACCGCCGGGTTGGCCCACCACaa                                                                                                               >  1:552325/1‑133 (MQ=255)      agtaAGTGCCGGTAGCGATGGGGAAAGCGGAGACGGCCCGTTTGCCGTGATCAAACACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTTCCCCCCCTTTCACCCCCGGGTAGGCCCACCACAAGaa                                                                                                            >  1:246257/1‑133 (MQ=255)      agtaAGTGCCGGTAGCGATGGGGAAAGCGGAGACGGCCCGTTTGCCGTAATCAAACACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTTCCCCCCCTTTCACCGCCGGGTTGGCCCACCACAAGaa                                                                                                            >  1:599046/1‑133 (MQ=255)             gCCGGTAGCGATGGGGAAAGCGGAGACGGCCCGTTTGCCGTGATCAAACACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTTCCCCCCCTTTCACCGCCGGGTTGGCCCACCACAAGAAATGAcgc                                                                                                     >  1:608077/1‑133 (MQ=255)                  tAGCGATGGGGAAAGCGGAGACGGCCCGTTTGCCGTGATCAAACACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTTCCCCCCCATTCACCGCCGGGATGGGCCACCACAAGAAAGGACGCggcgt                                                                                                >  2:217487/1‑133 (MQ=255)                  gaGCGACGGGGAAAACGGATACGGCCCGCTTCCCGTGATTAAACACCGCCGTTACAATCGGAGGGAAGACGCTCTGATGCCCTTCCCCCCCCTTCACCGCGGGGCTAGCGCACCACACGAAACAACgcggcgg                                                                                                >  2:304961/2‑132 (MQ=255)                                gCGGAGACGGCCCGTTTGCCGTGATCAAACACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTTCCCCCCCTTTCACCGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTtat                                                                                  >  2:153072/1‑133 (MQ=255)                                    agaCGGCCCGTTTGCCGTGATCAAACACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTTCCCCCCCTTTCACCGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGAc                                                                              >  1:604731/1‑133 (MQ=255)                                      aCGGCCCGTTTGCCGTGATCAAACACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTTCCCCCCCTTTCACCGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGAcgc                                                                            >  2:473752/1‑133 (MQ=255)                                          cccGTTTGCCGTGATCAAACACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTTCCCCCCCTTTCACCGCCGGGTTGCCCCACCACAAGAAATGACCCGGCGTGGCGTCCCGGTTATATACGCGGCt                                                                        >  1:257418/1‑133 (MQ=255)                                                tGCCGTGATCAAACACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTTCCCCCCCTTTCACCGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGAt                                                                  >  2:428088/1‑133 (MQ=255)                                                                  ccgTTACATCCGGCGGGAAGACGCTCTGATGCCCTTCCCCCCCTTTCACCGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTc                                                <  1:149412/133‑1 (MQ=255)                                                                  ccgTTACATCCGGCGGGAAGACGCTCTGATGCCCTTCCCCCCCTTTCACCGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTc                                                >  2:397106/1‑133 (MQ=255)                                                                  ccgTTACATCCGGCGGGAAGACGCTCTGATGCCCTTCCCCCCCTTTCACCGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTc                                                <  1:473752/133‑1 (MQ=255)                                                                  ccgTTACATCCGGCGGGAAGACGCTCTGATGCCCTTCCCCCCCTTTCACCGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGAGGCTGGCGATTTACAGCCCCGCCCGGTc                                                >  2:228874/1‑133 (MQ=255)                                                                    gTTACATCCGGCGGGAAGACGCTCTGATGCCCTTCCCCCCCTTTCACCGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGGGGCGTTCCCGTTATAGACCCGGCTGGCGATTTCCAGCGCCCCCCGGTCAg                                              >  2:480575/1‑133 (MQ=255)                                                                     ttACATCCGGCGGGAAGACGCTCTGATGCCCTTCCCCCCCTTTCACCGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAgg                                             >  1:333163/1‑133 (MQ=255)                                                                     ttACATCCGGCGGGAAGACGCTCTGATGCCCTTCCCCCCCTTTCACCGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAgg                                             >  2:524315/1‑133 (MQ=255)                                                                      tACATCCGGCGGGAAGACGCTCTGATGCCCTTCCCCCCCTTTCACCGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAggg                                            <  1:524313/133‑1 (MQ=255)                                                                        cATCCGGCGGTAAGACGCTCTGATCCCCCTCCCCCCCTTTCTCCGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAGGgcg                                          <  2:262962/133‑1 (MQ=255)                                                                        cATCCGGCGGGAAGACGCTCTGATGCCCTTCCCCCCCTTTCACCGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAGGgcg                                          <  2:604731/133‑1 (MQ=255)                                                                        cATCCGGCGGGAAGACGCTCTGATGCCCTTCCCCCCCTTTCACCGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCCGGCGATTTCCAACGCCGCCCGGTCAGGgcg                                          >  1:262962/1‑133 (MQ=255)                                                                         aTCCGGCGGGAAGACGCTCTGATGCCCTTCCCCCCCTTTCACCGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAGGgcgc                                         <  2:599046/133‑1 (MQ=255)                                                                         aTCCGGCGGGAAGACGCTCTGATGCCCTTCCCCCCCTTTCACCGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAGGgcgc                                         <  1:153072/133‑1 (MQ=255)                                                                         aTCCGGCGGGAAGACGCTCTGATGCCCTTCCCCCCCTTTCACCGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAGGgcgc                                         <  2:350206/133‑1 (MQ=255)                                                                             ggcggGAAGACGCTCTGATGCCCTTCCCCCCCTTTCACCGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGGTTTCCAGGGCCCCCCGGTCAGAGCGCAggg                                     >  1:214847/1‑133 (MQ=255)                                                                                   aaGACGCTCTGATGCCCTTCCCCCCCTTTCACCGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAGGGCGCAGGGTGAAAc                               <  1:524315/133‑1 (MQ=255)                                                                                     gACGCTCTGATGCCCTTCCCCCCCTTTCACCGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAGGGCGCAGGGTGAAACCt                             >  2:489804/1‑133 (MQ=255)                                                                                     gACGCTCTGATGCCCTTCCCCCCCTTTCACCGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAGGGCGCAGGGTGAAACCt                             <  2:552325/133‑1 (MQ=255)                                                                                        gCTCTGATACCCTTCTCCCCCTTTCACCGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTTGCGATTTCCAGCGCCGCCCGGTCAGGGCGCAGGGTGAAACCTGtc                          <  1:217487/133‑1 (MQ=255)                                                                                          tctGATGCCCTCCCCCCCCTTTCACCGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAGGGCGCAGGGTGAAACCTGtcat                        <  2:118972/133‑1 (MQ=255)                                                                                          tctGATGCCCGTCCCCCCCTTTCCCCGCTGGGTTGCCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAGGGCGCAGGGTGAAACCTGtcat                        <  2:257418/133‑1 (MQ=255)                                                                                                                cACCGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAGGGCGCAGGGTGAAACCTGTCATCACCTGTAAACCATGCATCGGc  <  1:321904/133‑1 (MQ=255)                                                                                                                   |                                                                                                                                  TTTGTAGTAAGTGCCGGTAGCGATGGGGAAAGCGGAGACGGCCCGTTTGCCGTGATCAAACACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTTCCCCCCCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAGGGCGCAGGGTGAAACCTGTCATCACCTGTAAACCATGCATCGGC  >  NC_000913/2682050‑2682293

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑