Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	368,127	G→A	G98G (GGC→GGT)	mhpR ←	DNA‑binding transcriptional activator MhpR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	368,127	0	G	A	100.0%	92.5 / NA	30	G98G (GGC→GGT)	mhpR	DNA‑binding transcriptional activator MhpR
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (20/10);  total (20/10)

GCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGCTGAAACGGTGAGTGGTTTCGCGTACCACCATTGCATCAACATCCAGCGTGGACACATCTGTCGGCCATACCACTTCGCGCAACAGATCGCCCAGCAGTGGGGCCGCCAGTGCAGAAATCCACTGTTCGTCACGAAATCCTTCGCTTAATTGCCGCACTTTGATGGTCAGTCGAAAACTATCATCGGAGGGGCTACGGCGGACATATCCCT  >  NC_000913/367997‑368248                                                                                                                                   |                                                                                                                          gCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGCTGAAACGGTGAGTGGTTTCGCGTACCACCATTGCATCAACATCCAGCGTGGACACATCTGTCGGCCATACCACTTCGCGCAACAGATCAcc                                                                                                                         >  2:454627/1‑133 (MQ=255)  cAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGCTGAAACGGTGAGTGGTTTCGCGTACCACCATTGCATCAACATCCAGCGTGGACACATCTGTCGGCCATACCACTTCGCGCAACAGATCAccc                                                                                                                        <  1:425586/133‑1 (MQ=255)            ccGACCATTGCCCGGTGAAAGGATAAGCGGCTGAAACGGTGAGTGGTTTCGCGTACCACCATTGCATCAACATCCAGCGTGGACACATCTGGCGGCCATACCACCTCGCGCAACAGAACACCGAGCAGTgggg                                                                                                              >  2:203364/1‑133 (MQ=255)                           tGAAAGGATAAGCGGCTGAAACGGTGAGTGGTTTCGCGTACCACCATTGCATCAACATCCAGCGTGGACACATCTGTCGGCCATACCACTTCGCGCAACAGATCACCCAGCAGTGGGGCCGCCAGTGCAGaaa                                                                                               >  1:633215/1‑133 (MQ=255)                                     aGCGGCTGAAACGGTGAGTGGTTTCGCGTACCACCATTGCATCAACATCCAGCGTGGACACATCTGTCGGCCATACCACTTCGCGCAACAGATCACCCAGCAGTGGGGCCGCCAGTGCAGAAATCCACTGTTc                                                                                     >  2:82002/1‑133 (MQ=255)                                      gCGGCTGAAACGGTGAGTGGTTTCGCGTACCACCATTGCATCAACATCCAGCGTGGACACATCTGTCGGCCATACCACTTCGCGCAACAGATCACCCAGCAGTGGGGCCGCCAGTGCAGAAATCCACTGTTCg                                                                                    <  1:642849/133‑1 (MQ=255)                                                           ttCGCGTACCACCATTGCATCAACATCCAGCGTGGACACATCTGTCGGCCATACCACTTCGCGCAACAGATCACCCAGCAGTGGGGCCGCCAGTGCAGAAATCCACTGTTCGTCACGAAATCCTTCGCTTAAt                                                               >  2:101779/1‑133 (MQ=255)                                                            tCGCGTACCACCATTGCATCAACATCCAGCGTGGACACATCTGTCGGCCATACCACTTCGCGCAACAGATCACCCAGCAGTGGGGCCGCCAGTGCAGAAATCCACTGTTCGTCACGAAATCCTTCGCTTAAtt                                                              >  2:377768/1‑133 (MQ=255)                                                               cgTACCACCATTGCATCAACATCCAGCGTGGACACATCTGTCGGCCATACCACTTCGCGCAACAGATCACCCAGCAGTGGGGCCGCCAGTGCAGAAATCCACTGTTCGTCACGAAATCCTTCGCTTAATTGcc                                                           >  1:559759/1‑133 (MQ=255)                                                                 tACCACCATTGCATCAACATCCAGCGTGGACACATCTGTCGGCCATACCACTTCGCGCAACAGATCACCCAGCAGTGGGGCCGCCAGTGCAGAAATCCACTGTTCGTCACGAAATCCTTCGCTTAATTGCCGc                                                         >  1:496871/1‑133 (MQ=255)                                                                              tCAACATCCAGCGTGGACACATCTGTCGGCCATACCACTTCGCGCAACAGATCACCCAGCAGTGGGGCCGCCAGTGCAGAAATCCACTGTTCGTCACGAAATCCCTGGGCTAATTGCCGCACTGTGATGGTCa                                            >  2:297091/1‑133 (MQ=255)                                                                                 aCATCCAGCGTGGACACATCTGTCGGCCATACCACTTCGCGCAACAGATCACCCAGCAGTGGGGCCGCCAGTGCAGAAATCCACTGTTCGTCACGAAATCTTTCTCTTAATTGCCGCCCTTTGATGGTCAGTc                                         >  2:253097/1‑133 (MQ=255)                                                                                 aCATCCAGCGTGGACACATCTGTCGGCCATACCACTTCGCGCAACAGATCACCCAGCAGTGGGGCCGCCAGTGCAGAAATCCACTGTTCGTCACGAAATCCTTCGCTTAATTGCCGCACTTTGATGGTCAGTc                                         >  2:123667/1‑133 (MQ=255)                                                                                  cATCCAGCGTGGACACATCTGTCGGCCATACCACTTCGCGCAACAGATCACCCAGCAGTGGGGCCGCCAGTGCAGAAATCCACTGTTCGTCACGAAATCCTTCGCTTAATTGCCGCACTTTGATGGTCAGTCg                                        <  1:377768/133‑1 (MQ=255)                                                                                    tCCAGCGTGGACACATCTGTCGGCCATACCACTTCGCGCAACAGATCACCCAGCAGTGGGGCCGCCAGTGCAGAAATCCACTGTTCGTCACGAAATCCTTCGCTTAATTGCCGCACTTTGATGGTCAGTCGaa                                      <  1:12872/133‑1 (MQ=255)                                                                                    tCCAGCGTGGACACATCTGTCGGCCATACCACTTCGCGCAACAGATCACCCAGCAGTGGGGCCGCCAGTGCAGAAATCCACTGTTCGTCACGAAATCCTTCGCTTAATTGCCGCACTTTGATGGTCAGTCGaa                                      >  1:91756/1‑133 (MQ=255)                                                                                    tCCAGCGTGGACACATCTGTCGGCCATACCACTTCGCGCAACAGATCACCCAGCAGTGGGGCCGCCAGTGCAGAAATCCACTGTTCGTCACGAAATCCTTCGCTTAATTGCCGCACTTTGATGGTCAGTCGaa                                      >  2:12872/1‑133 (MQ=255)                                                                                     ccAGCGTGGACACATCTGTCGGCCATACCACTTCGCGCAACAGATCACCCAGCAGTGGGGCCGCCAGTGCAGAAATCCACTGTTCGTCACGAAATCCTTCGCTTAATTGCCGCACTTTGATGGTCAGTCGaaa                                     <  1:454627/133‑1 (MQ=255)                                                                                         cGTGGACACATCTGTCGGCCATACCACTTCGCGCAACAGATCACCCAGCAGTGGGGCCGCCAGTGCAGAAATCCACTGTTCGTCACGTAATCCTTCGCTTAATTGCGGCACTTTGATGGTCAGGCGAAAACTa                                 >  2:275139/1‑133 (MQ=255)                                                                                            gtacacATCTGTCGGCCATACCACTTCGCGCAACAGATCACCCAGCAGTGGGGCCGCCAGTGCAGAAATCCACTGTTCGTCACGAAATCCTTCGCTTAATTGCCGCACTTTGATGGTCAGTCGAAAACTatca                              >  1:414141/3‑133 (MQ=255)                                                                                             gACACATCTGTCGGCCATACCACTTCGCGCAACAGATCACCCAGCAGTGGGGCCGCCAGTGCAGAAATCCACTGTTCGTCACGAAATCCTTCGCTTAATTGCCGCACTTTGATGGTCAGTCGAAAACTatcat                             >  1:455083/1‑133 (MQ=255)                                                                                             gACACATCTGTCGGCCATACCACTTCGCGCAACAGATCACCCAGCAGTGGGGCCGCCAGTGCAGAAATCCACTGTTCGTCACGAAATCCTTCGCTTAATTGCCGCACTTTGATGGTCAGTCGAAAACTatcat                             <  2:639671/133‑1 (MQ=255)                                                                                             gACACATCTGTCGGCCATACCACTTCGCGCAACAGATCACCCAGCAGTGGGGCCGCCAGTGCAGAAATCCACTGTTCGTCACGAAATCCTTCGCTTAATTGCCGCACTTTGATGGTCAGTCGAAAACTatcat                             >  1:33312/1‑133 (MQ=255)                                                                                             gACACATCTGGCGGCCATACCACTTCGCTCAACAGATCCCCCAGCAGTGGGGCCGCCAGTGCAGAAATCCACTGTTCGTCACGAAATCCTTCGCTTAATTGCCGCACTTTGATGGTCAGTCGAAAACTatcat                             <  2:227890/133‑1 (MQ=255)                                                                                                acaTCTGTCGGCCATACCACTTCGCGCAACAGATCACCCAGCAGTGGGGCCGCCAGTGCAGAAATCCACTGTTCGTCACGAAATCCTTCGCTTAATTGCCGCACTTTGATGGTCAGTCGAAAACTATCATCgg                          <  2:486802/133‑1 (MQ=255)                                                                                                       tCGGCCATACCACTTCGCGCAACAGATCACCCAGCAGTGGGGCCGCCAGTGCAGAAATCCACTGTTCGTCACGAAATCCTTCGCTTAATTGCCGCACTTTGATGGTCAGTCGAAAACTATCATCGGAGGGGCt                   >  1:423873/1‑133 (MQ=255)                                                                                                       tCGGCCATACCACTTCGCGCAACAGATCACCCAGCAGTGGGGCCGCCAGTGCAGAAATCCACTGTTCGTCACGAAATCCTTCGCTTAATTGCCGCACTTTGATGGTCAGTCGAAAACTATCATCGGAGGGGCt                   >  1:404066/1‑133 (MQ=255)                                                                                                          gCCATACCGCTTCGCGCAGCAGGTCACCCAGCAGTGGGGCCGCCAGTGCAGAAATCCACTGTTCGTCACGAAATCCTTCGCTTAATTGCCGCACTTTGATGGTCAGTCGAAAACTATCATCGGAGGGGCTAcg                <  1:297091/133‑1 (MQ=255)                                                                                                            cATACCACTTCGCGCAACAGATCACCCAGCAGTGGGGCCGCCAGTGCAGAAATCCACTGTTCGTCACGAAATCCTTCGCTTAATTGCCGCACTTTGATGGTCAGTCGAAAACTATCATCGGAGGGGCTAcggc              <  1:82002/133‑1 (MQ=255)                                                                                                              tACCACTTAGCGCAACAGATCAGCCAGCAGTGGGGCCGCCAGTGCAGAAATCCACTGTTCGTCACGAAATCCTTCGCTTAATTGCCGCACTTTGATGGTCAGTCGAAAACTATCATCGGAGGGGCTAcggcgg            <  1:266567/133‑1 (MQ=255)                                                                                                                 cACTTCGCCCAACAGCTCACCCAGCAGTGGAGCCGCCAGTGCAGAAATCCACTGTTCGTCACGAAATCCTTCGCTTAATTGCCGCACTTTGATGGTCAGTCGAAAACTATCATCGGAGGGGCTACGGCGGACa         <  1:203364/133‑1 (MQ=255)                                                                                                                     tCGCGCAACAGATCACCCAGCAGTGGGGCCGCCAGTGCAGAAATCCACTGTTCGTCACGAAATCCTTCGCTTAATTGCCGCACTTTGATGGTCAGTCGAAAACTATCATCGGAGGGGCTACGGCGGACATATc     >  2:40463/1‑133 (MQ=255)                                                                                                                        cgcAACAGATCACCCAGCAGTGGGGCCGCCAGTGCAGAAATCCACTGTTCGTCACGAAATCCTTCGCTTAATTGCCGCACTTTGATGGTCAGTCGAAAACTATCATCGGATGGGCTACGGCGGACATATcctt  >  2:550759/1‑131 (MQ=255)                                                                                                                                   |                                                                                                                          GCAAACGTCGCCCGACCATTGCCCGGTGAAAGGATAAGCGGCTGAAACGGTGAGTGGTTTCGCGTACCACCATTGCATCAACATCCAGCGTGGACACATCTGTCGGCCATACCACTTCGCGCAACAGATCGCCCAGCAGTGGGGCCGCCAGTGCAGAAATCCACTGTTCGTCACGAAATCCTTCGCTTAATTGCCGCACTTTGATGGTCAGTCGAAAACTATCATCGGAGGGGCTACGGCGGACATATCCCT  >  NC_000913/367997‑368248

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑