Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,926,701	G→C	V132L (GTC→CTC)	ppnN →	nucleotide 5'‑monophosphate nucleosidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,926,701	0	G	C	100.0%	58.4 / NA	17	V132L (GTC→CTC)	ppnN	nucleotide 5'‑monophosphate nucleosidase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (9/8);  total (9/8)

ACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATCACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGCGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGAAACCAGCT  >  NC_000913/2926594‑2926828                                                                                                            |                                                                                                                                aCTTGTTCGCAGTCCCGCGTGACACTCTCTTCCTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATCACTAACCTGCTCTTTTCCATCTTGCGTAACGCTcg                                                                                                        <  2:168590/133‑1 (MQ=255)   ttGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATCACTAACCTGCTCTTTTCCATCTTGCGTAACGCTcgcg                                                                                                      <  1:412591/133‑1 (MQ=255)                       cATTCTCTTCTTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATCACTAACCTGCTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAgcg                                                                                  <  2:254115/133‑1 (MQ=255)                       cATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATCACTAACCTGCTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAgcg                                                                                  >  1:462632/1‑133 (MQ=255)                                   ttACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATCACTAACCTGCTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATAtggtg                                                                      <  2:554095/133‑1 (MQ=255)                                     aCGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATCACTAACCTGCTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAAAATggtggt                                                                    >  1:228223/1‑133 (MQ=255)                                                        cgttcgttTTCCCAACCTGAATCTCGACAACTCCGTCCACATCACTAACCTGCTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGCGGTCACTCa                                                 >  2:492848/1‑133 (MQ=255)                                                              ttttCCCAACCTGAATCTCGACAACTCCGTCCACATCACTAACCTGCTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGCGGTCACTCAATTAAc                                           <  1:343627/133‑1 (MQ=255)                                                                    cAACCTGAATCTCGACAACTCCGTCCACATCACTAACCTGCTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGCGGTCACTCAATTAACGAAAAc                                     <  2:462632/133‑1 (MQ=255)                                                                                   cAACTCCGTCCACATCACTAACCTGCTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGCGGTCACTCAATTAACGAAAACGAGTATTTGTATGcc                      >  2:339534/1‑133 (MQ=255)                                                                                     aCTCCGTCCACATCACTAACCTGCTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGCGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCcg                    >  1:46415/1‑133 (MQ=255)                                                                                           tCCACATCACTAACCTGCTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGCGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCcgtcgcgt              >  2:553699/1‑133 (MQ=255)                                                                                                aTCACTAACCTGCTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGCGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGaa         >  2:116245/1‑133 (MQ=255)                                                                                                       aCCTGCTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGCGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGAAACCAGCt  >  1:47306/1‑133 (MQ=255)                                                                                                       aCCTGCTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGCGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGAAACCAGCt  <  2:47306/133‑1 (MQ=255)                                                                                                        ccTGCTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGCGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGaa         <  1:544442/125‑1 (MQ=255)                                                                                                        ccTGCTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGCGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGaa         >  2:544442/1‑125 (MQ=255)                                                                                                            |                                                                                                                                ACTTGTTCGCGGTCCTGCGTGACATTCTCTTCGTTTACGGGCAAATCCATAACACCGTTCGTTTTCCCAACCTGAATCTCGACAACTCCGTCCACATCACTAACCTGGTCTTTTCCATCTTGCGTAACGCTCGCGCGCTGCATGTGGGTGAAGCGCCAAATATGGTGGTCTGCTGGGGCGGTCACTCAATTAACGAAAACGAGTATTTGTATGCCCGTCGCGTCGGAAACCAGCT  >  NC_000913/2926594‑2926828

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑