Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,025,101	G→A	G251S (GGC→AGC)	xanQ →	xanthine:H(+) symporter XanQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,025,101	0	G	A	100.0%	65.7 / NA	20	G251S (GGC→AGC)	xanQ	xanthine:H(+) symporter XanQ
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (11/9);  total (11/9)

GGAGGGATCGCCATTGGGCTATGTGTCGGCTATATCGCATCGTTATGCCTGGGCATGGTGGATTTCAGCAGTATGCGCAATTTGCCGTTAATCACCATCCCGCATCCGTTCAAATACGGCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCGCCACGGCAATGGTTTCCCGCCGCCCCATTCA  >  NC_000913/3024984‑3025216                                                                                                                      |                                                                                                                    ggagggaTCGCCATTGGGCTATGTGTCGGCTATATCGCATCGTTATGCCTGGGCATGGTGGATTTCAGCAGTATGCGCAATTTGCCGTTAATCACCATCCCGCATCCGTTCAAATACAGCTTTAGTTTTAGCt                                                                                                      <  1:352876/133‑1 (MQ=255)  gagggaTCGCCATTGGGCTATGTGTCGGCTATATCGCATCGTTATGCCTGGGCATGGTGGATTTCAGCAGTATGCGCAATTTGCCGTTAATCACCATCCCGCCTCCGTTCAAATACAGCTTtagctttagctt                                                                                                     >  1:208829/1‑133 (MQ=255)  gagggaTCGCCATTGGGCTATGTGTCGGCTATATCGCATCGTTATGCCTGGGCATGGTGGATTTCAGCAGTATGCGCAATTTGCCGTTAATCACCATCCCGCATCCGTTCAAATACAGCTTTAGTTTTAGCtt                                                                                                     <  2:42973/133‑1 (MQ=255)     ggaTCGCCATTGGGCTATGTGTCGGCTATATCGCATCGTTATGCCTGGGCATGGTGGATTTCAGCAGTATGCGCAATTTGCCGTTAATCACCATCCCGCATCCGTTCAAATACAGCTTTAGTTTTAGCTTCCa                                                                                                  >  1:583779/1‑133 (MQ=255)      gaTCGCCATTGGGCTATGTGTCGGCTATATCGCATCGTTATGCCTGGGCATGGTGGATTTCAGCAGTATGCGCAATTTGCCGTTAATCACCATCCCGCATCCGTTCAAATACAGCTTTAGTTTTAGCTTCCAt                                                                                                 <  1:421336/133‑1 (MQ=255)       aTCGCCATTGGGCTATGTGTCGGCTATATCGCATCGTTATGCCTGGGCATGGTGGATTTCAGCAGTATGCGCAATTTGCCGTTAATCACCATCCCGCATCCGTTCAAATACAGCTTTAGTTTTAGCTTCCATc                                                                                                >  2:530501/1‑133 (MQ=255)                   cTATGTGTCGGCTATATCGCATCGTTATGCCTGGGCATGGTGGATTTCAGCAGTATGCGCAATTTGCCGTTAATCACCATCCCGCATCCGTTCAAATACAGCTTTAGTTTTAGCTTCCATCAGTTCCtggtgg                                                                                    <  2:377784/133‑1 (MQ=255)                       gtgtCGGCTATATCGCATCGTTATGCCTGGGCATGGTGGATTTCAGCAGTATGCGCAATTTGCCGTTAATCACCATCCCGCATCCGTTCAAATACAGCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTgg                                                                                <  1:530501/133‑1 (MQ=255)                         gtCGGCTATAGCGCATCGTTATGCCTGGTCATCGTGGATTTTAGCAGTATGCGCAATTTGCCGTTAATCACCATCCCGCATCCGTTCAAATACAGCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCa                                                                              <  2:208829/133‑1 (MQ=255)                            ggCTATATCGCATCGTTATGCCTGGGCATGGTGGATTTCAGCAGTATGCGCAATTTGCCGTTAATCACCATCCCGCATCCGTTCAAATACAGCTTTAGTTTTAGCTTCCAACAGATCGTGGTGGGTGGCACGa                                                                           >  1:248692/1‑133 (MQ=255)                                 tatCGCATCGTTATGCCTGGGCATGGTGGATTTCAGCAGTATGCGCAATTTGCCGTTAATCACCATCCCGCATCCGTTCAAATACAGCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTAt                                                                      <  1:555013/133‑1 (MQ=255)                                                ccTGGGCATGGTGGATTTCAGCAGTATGCGCAATTTGCCGTTAATCACCATCCCGCATCCGTTCAAATACAGCTTTAGTTTTAGCTTCCATGAGTTCCTGCTGGGTGGGACGATTTATCTGCATAGCGTGCTg                                                       >  2:174896/1‑133 (MQ=255)                                                   gggCATGGTGGATTTCAGCAGTATGCGCAATTTGCCGTTAATCACCATCCCGCATCCGTTCAAATACAGCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGaa                                                    >  2:572991/1‑133 (MQ=255)                                                         ggtggATTTCAGCAGTATGCGCAATTTGCCGTTAATCACCATCCCGCATCCGTTCAAATACAGCTTTAGTTTTAGCTTACATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAg                                                <  2:137941/131‑1 (MQ=255)                                                         ggtggATTTCAGCAGTATGCGCAATTTGCCGTTAATCACCATCCCGCATCCGTTCAAATACAGCTTTAGTTTTAGCTTACATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAg                                                >  1:137941/1‑131 (MQ=255)                                                          gtggATTTCAGCAGTATGCGCAATTTGCCGTTAATCACCATCCCGCATCCGTTCAAATACAGCTTTAGTTTTAGCTTCCATCAGTTCCTGGAGGTTGGCACGATATATTTGCTTAGCTTGCTAGAAGCAGTCg                                             >  2:203399/1‑133 (MQ=255)                                                               tttCAGCAATATGCGCAATTTGCCGTTAATAACCATCCCGCATCCGTTCAAATACAGCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGat                                        <  1:174896/133‑1 (MQ=255)                                                                     cagTATGCGCAATTTGCCGTTAATCACCATCCCGCATCCGTTCAAATACAGCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCAcc                                  >  1:336682/1‑133 (MQ=255)                                                                                                  tCCCGCATCCGTTCAAATACAGCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCGCCACGGCAATGGTTTCCCGCCGCCCCAt     >  2:437618/1‑133 (MQ=255)                                                                                                     cGCATCCGTTCAAATACAGCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCGCCACGGCAATGGTTTCCCGCCGCCCCATTCa  >  1:22299/1‑133 (MQ=255)                                                                                                                      |                                                                                                                    GGAGGGATCGCCATTGGGCTATGTGTCGGCTATATCGCATCGTTATGCCTGGGCATGGTGGATTTCAGCAGTATGCGCAATTTGCCGTTAATCACCATCCCGCATCCGTTCAAATACGGCTTTAGTTTTAGCTTCCATCAGTTCCTGGTGGTTGGCACGATTTATCTGCTTAGCGTGCTGGAAGCAGTCGGCGATATCACCGCCACGGCAATGGTTTCCCGCCGCCCCATTCA  >  NC_000913/3024984‑3025216

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑