Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,216,334	T→C	H53R (CAC→CGC)	nfeF ←	NADPH‑dependent ferric chelate reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,216,334	0	T	C	100.0%	108.0 / NA	31	H53R (CAC→CGC)	nfeF	NADPH‑dependent ferric chelate reductase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (18/13);  total (18/13)

ACAGCGGCGTATAGTCACGCGACGGTGGGCGTGGTCCTTCCGGCCAGACGATGCCCTCTTCCGTTACCGTTGGCGGCACAAAGTGAGCGTCAGGTTGAGGAAAGAAGAGTTTGCTGTGATCGTCAAAGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTCAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAAC  >  NC_000913/3216218‑3216461                                                                                                                     |                                                                                                                                aCAGCGGCGTATAGTCACGCGACGGTGGGCGTGGTCCTTCCGGCCAGACGATGCCCTCTTCCGTTACCGTTGGCGGCACAAAGTGAGCGTCAGGTTGAGGAAAGAAGAGTTTGCTGCGATCGTCAAAGCCAcg                                                                                                                 <  1:70975/133‑1 (MQ=255)   aGCGGCGTATAGTCACGCGACGGTGGGCGTGGTCCTTCCGGCCAGACGATGCCCTCTTCCGTTACCGTTGGCGGCACAAAGTGAGCGTCAGGTTGAGGAAAGTAGAGTTTGCTGCGATCGTCAAAGCCAcgcg                                                                                                               >  1:291613/1‑133 (MQ=255)     cggcgTATAGTCACGCGACGGTGGGCGTGGTCCTTCCGGCCAGACGATGCCCTCTTCCGTTACCGTTGGCGGCACAAAGTGAGCGTCAGGTTGAGGAAAGAAGAGTTTGCTGCGATCGTCAAAGCCAcg                                                                                                                 <  1:102924/129‑1 (MQ=255)     cggcgTATAGTCACGCGACGGTGGGCGTGGTCCTTCCGGCCAGACGATGCCCTCTTCCGTTACCGTTGGCGGCACAAAGTGAGCGTCAGGTTGAGGAAAGAAGAGTTTGCTGCGATCGTCAAAGCCAcg                                                                                                                 >  2:102924/1‑129 (MQ=255)          tataGTCACGCGACGGTGGGCGTGGTCCTTCCGGCCAGACGATGCCCTCTTCCGTTACCGTTGGCGGCACAAAGTGAGCGTCAGGTTGAGGAAAGAAGAGTTTGCTGCGATCGTCAAAGCCACGCGATGTaaa                                                                                                        >  1:385111/1‑133 (MQ=255)                 aCGCGACGGTGGGCGTGGTCCTTCCGGCCAGACGATGCCCTCTTCCGTTACCGTTGGCGGCACAAAGTGAGCGTCAGGTTGAGGAAAGAAGAGTTTGCTGCGATCGTCAAAGCCACGCGATGTAAAACCGTcc                                                                                                 >  2:157801/1‑133 (MQ=255)                    cgACGGTGGGCGTGGTCCTTCCGGCCAGACGATGCCCTCTTCCGTTACCGTTGGCGGCACAAAGTGAGCGTCAGGTTGAGGAAAGAAGAGTTTGCTGCGATCGTCAAAGCCACGCGATGTAAAACCGTCCAgc                                                                                              >  1:602521/1‑133 (MQ=255)                          tGGGCGTGGTCCTTCCGGCCAGACGATGCCCTCTTCCGTTACCGTTGGCGGCACAAAGTGAGCGTCAGGTTGAGGAAAGAAGAGTTTGCTGCGATCGTCAAAGCCACGCGATGTAAAACCGTCCAGCGCCTcg                                                                                        <  2:642683/133‑1 (MQ=255)                          tGGGCGTGGTCCTTCCGGCCAGACGATGCCCTCTTCCGTTACCGTTGGCGGCACAAAGTGAGCGTCAGGTTGAGGAAAGAAGAGTTTGCTGCGATCGTCAAAGCCACGCGATGTAAAACCGTCCAGCGCCTcg                                                                                        <  1:411488/133‑1 (MQ=255)                             gCGTGGTCCTTCCGGCCAGACGATGCCCTCTTCCGTTACCGTTGGCGGCACAAAGTGAGCGTCAGGTTGAGGAAAGAAGAGTTTGCTGCGATCGTCAAAGCCACGCGATGTAAAACCGTCCAGCGCCTcgccg                                                                                     >  2:10143/1‑133 (MQ=255)                               gTGGTCCTTCCGGCCAGACGATGCCCTCTTCCGTTACCGTTGGCGGCACAAAGTGAGCGTCAGGTTGGGGAAAGAAGAGTTTGCTGCGATCGTCAAAGCCACGCGATGTAAAACCGTCCAGCGCCTCgccgcc                                                                                   >  1:621406/1‑133 (MQ=255)                                            ccAGACGATGCCCTCTTCCGTTACCGTTGGCGGCACAAAGTGAGCGTCAGGTTGAGGAAAGAAGAGTTTGCTGCGATCGTCAAAGCCACGCAATGTAAAAACGTGCAGGGCCTCGCCGCCGAAGACAATgcgc                                                                      >  2:258929/1‑133 (MQ=255)                                                     gCCCTCTTCCGTTACCGTTGGCGGCACAAAGTGAGCGTCAGGTTGAGGAAAGAAGAGTTTGCTGCGATCGTCAAAGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCg                                                             >  1:408045/1‑133 (MQ=255)                                                        ctctTCCGTTACCGTTGGCGGCACTAAGCGAGCGTCAGGTTTAGGAAAGAAGAGTTTGCTGCGATCGTCAAAGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGgcg                                                          <  2:291613/133‑1 (MQ=255)                                                          ctTCCGTTACCGTTGGCGGCACAAAGTGAGCGTCAGGTTGAGGAAAGAAGAGTTTGCTGCGATCGTCAAAGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGgcg                                                          <  1:17743/131‑1 (MQ=255)                                                          ctTCCGTTACCGTTGGCGGCACAAAGTGAGCGTCAGGTTGAGGAAAGAAGAGTTTGCTGCGATCGTCAAAGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGgcg                                                          >  2:17743/1‑131 (MQ=255)                                                             ccGTTACCGTTGGCGGCACAAAGTGAGCGTCAGGTTGAGGAAAGAAGAGTTTGCTGCGATCGTCAAAGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCTGAt                                                     <  2:385111/133‑1 (MQ=255)                                                                    cGTTGGCGGCACAAAATGAGCGTCAGGTTGAGCAAAGAAGAGTTTGCTGCGATCGTCAAAGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTCa                                              <  2:263598/133‑1 (MQ=255)                                                                         gcggcACAAAGTGAGCGTCAGGTTGAGGAAAGAAGAGTTTGCTGCGATCGTCAAAGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTCAAcgcg                                         >  2:473269/1‑133 (MQ=255)                                                                             cacaAAGTGAGCGTCAGGTTGAGGAAAGAAGAGTTTGCTGCGATCGTCAAAGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTCAACGCGTAac                                     >  1:425548/1‑133 (MQ=255)                                                                                  aGTGAGCGTCAGGTTGAGGAAAGAAGAGTTTGCTGCGATCGTCAAAGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTCAACGCGTAACAcagt                                <  1:604796/133‑1 (MQ=255)                                                                                    tGAGCGTCAGGTTGGGGAAAGAAGAGTTTGCTGCGATCGTCAAAGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTCAACGCGTAACAcagtca                              <  2:621406/133‑1 (MQ=255)                                                                                       gCGTCAGGTTGAGGAAAGAAGAGTTTGCTGCGATCGTCAAAGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTCAACGCGTAACACAGTCAGtt                           >  2:83926/1‑133 (MQ=255)                                                                                        cGTCAGGTTGAGGAAAGAAGAGTTTGCTGCGATCGTCAAAGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTCAACGCGTAACGCAGTCAGTTc                          >  2:339345/1‑133 (MQ=255)                                                                                           cAGGTTGAGGAAAGAAGAGTTTGCTGCGATCGTCAAAGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCTGAAGCGCTCATCGCGTACCACAGTCAGTTCACg                       >  1:250350/1‑133 (MQ=255)                                                                                            aGGTTGAGGAAAGAAGAGTTTGCTGCGATCGTCAAAGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTCAACGCGTAACACAGTCAGTTCACgg                      <  2:408045/133‑1 (MQ=255)                                                                                            aGGTTGAGGAAAGAAGAGTTTGCTGCGATCGTCAAAGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTCAACGCGTAACACAGTCAGTTCACgg                      <  2:602521/133‑1 (MQ=255)                                                                                            aGGTTGAGGAAAGAAGAGTTTGCTGCGATCGTCAAAGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTCAACGCGTAACACAGTCAGTTCACgg                      <  2:621983/133‑1 (MQ=255)                                                                                            aGGTTGAGGAAAGAAGAGTTTGCTGCGATCGTCAAAGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCAAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTCAACGCGTAACACAGTCAGTTCACgg                      >  1:420688/1‑133 (MQ=255)                                                                                              gTTGAGGAAAGAAGAGTTTGCTGCGATCGTCAAAGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTCAACGCGTAACACAGTCAGTTCACGGaa                    >  1:63119/1‑133 (MQ=255)                                                                                                              tttGCTGCGATCGTCAAAGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTCAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGa    >  2:480641/1‑133 (MQ=255)                                                                                                                tgctgcGATCGTCAAAGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTCAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAAc  >  2:426750/1‑133 (MQ=255)                                                                                                                     |                                                                                                                                ACAGCGGCGTATAGTCACGCGACGGTGGGCGTGGTCCTTCCGGCCAGACGATGCCCTCTTCCGTTACCGTTGGCGGCACAAAGTGAGCGTCAGGTTGAGGAAAGAAGAGTTTGCTGTGATCGTCAAAGCCACGCGATGTAAAACCGTCCAGCGCCTCGCCGCCGAGGACAATGCGCTGAAAACCGGCGCTGATGCGCTCAACGCGTAACACAGTCAGTTCACGGAAGCGCAGATCATTGCGAAC  >  NC_000913/3216218‑3216461

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑