Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,276,320	G→A	E347K (GAG→AAG)	garD →	galactarate dehydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,276,320	0	G	A	100.0%	144.8 / NA	45	E347K (GAG→AAG)	garD	galactarate dehydratase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (21/24);  total (21/24)

CTATGCGTCTGATCTACTGGTGCGCTGCGGCGCAACGGTGATGTTTTCAGAAGTAACGGAAGTGCGTGACGCGATCCATCTGCTGACACCACGCGCAGTGAACGAAGAGGTCGGCAAACGGCTGCTGGAGGAGATGGAGTGGTACGATAACTATCTCAATATGGGAAAAACCGACCGCAGCGCCAACCCTTCGCCGGGCAACAAGAAAGGCGGTCTGGCAAACGTGGTAGAGAAGGCACTCGGCTCCATTGCT  >  NC_000913/3276193‑3276445                                                                                                                                |                                                                                                                              cTATGCGTCTGATCTACTGGTGCGCTGCGGCGCAACGGTGATGTTTTCAGAAGTAACGGAAGTGCGTGACGCGATCCATCTGCTGACACCACGCGCAGTGAACGAAGAGGTCGGCAAACGGCTGCTGaaggag                                                                                                                          <  1:25110/133‑1 (MQ=255)  tATGCGTCTGATCTACTGGTGCGCTGCGGCGCAACGGTGATGTTTTCAGAAGTAACGGAAGTGCGTGACGCGATCCATCTGCTGACACCACGCGTAGTGAACGAAGAGGTCGGCAAACGGCTGCTGAAGgaga                                                                                                                         <  1:149708/133‑1 (MQ=255)  tATGCGTCTGATCTACTGGTGCGCTGCGGCGCAACGGTGATGTTTTCAGAAGTAACGGAAGTGCGTGACGCGATCCATCTGCTGACACCACGCGCAGTGATCGAAGAGGTCGGCAAACGGCTGCTGAAGgaga                                                                                                                         <  2:439098/133‑1 (MQ=255)  tATGCGTCTGATCTACTGGTGCGCTGCGGCGCAACGGTGATGTTTTCAGAAGTAACGGAAGTGCGTGACGCGATCCATCTGCTGACACCACGCGCAGTGAACGAAGAGGTCGGCAAACGGCTGCTGAAGgaga                                                                                                                         <  2:437978/133‑1 (MQ=255)       gTCTGATCTACTGGTGCGCTGCGGCGCAACGGTGATGTTTTCAGAAGTAACGGAAGTGCGTGACGCGATCCATCTGCTGACACCACGCGCAGTGAACGAAGAGGTCGGCAAACGGCTGCTGAAGGAGATGGAg                                                                                                                    <  1:364492/133‑1 (MQ=255)        tCTGATCTACTGGTGCGCTGCGGCGCAACGGTGATGTTTTCAGAAGTAACGGAAGTGCGTGACGCGATCCATCTGCTGACACCACGCGCAGTGAACGAAGAGGTCGGCAAACGGCTGCTGAAGGAGATGGAGt                                                                                                                   <  2:354937/133‑1 (MQ=255)            aTCTACTGGTGCGCTGCGGCGCAACGGTGATGTTTTCAGAAGTAACGGAAGTGCGTGACGCGATCCATCTGCTGACACCACGCGCAGTGAACGAAGAGGTCGGCAAACGGCTGCTGAAGGAGATGGAGTGGTa                                                                                                               <  2:359469/133‑1 (MQ=255)              cTACTGGTGCGCTGCCGCGCAACGTTGAGGTTCTCAGAAGTGACGGAAGTGCGTGACGCGATCCATCTGCTGACACCACGCGCAGTGAACGAAGAGGTCGGCAAACGGCTGCTGAAGGAGATGGAGTGGTACg                                                                                                             <  2:224837/133‑1 (MQ=255)                aCTGGTGCGCTGCGGCGCAACGGTGATGTTTTCAGAAGTAACGGAAGTGCGTGACGCGATCCATCTGCTGACACCACGCGCAGTGAACGAAGAGGTCGGCAAGCGGCTGCGGAAGGAGATGGAGGGGTACGAt                                                                                                           >  1:195308/1‑133 (MQ=255)                 cTGGTGCGCTGCGGCGCAACGGTGATGTTTTCAGAAGTAACGGAAGTGCGTGACGCGATCCATCTGCTGACACCACGCGCAGTGAACGAAGAGGTCGGCAAACGGCTGCTGAAGGAGATGGAGTGGTACGATa                                                                                                          >  1:40197/1‑133 (MQ=255)                  tGGTGCGCTGCGGCGGAACGGTAATGTTTTAAGAAGTAACGGAAGTGCGTGACGCGATCCATCTGCTGACACCACGCGCAGTGAACGAAGAGGTCGGCAAACGGCTGCTGAAGGAGATGGAGTGGTACGATaa                                                                                                         <  1:234487/133‑1 (MQ=255)                  tGGTGCGCTGCGGCGCAACGGTGATGTTTTCAGAAGTAACGGAAGTGCGTGACGCGATCCATCTGCTGACACCACGCGCAGTGAACGAAGAGGTCGGCAAACGGCTGCTGAAGGAGATGGAGTGGTACGATaa                                                                                                         <  1:78045/133‑1 (MQ=255)                      gcgcTGCGGCGCAACGGTGATGTTTTCAGAAGTAACGGAAGTGCGTGACGCGATCCATCTGCTGACACCACGCGCAGTGAACGAAGAGGTCGGCAAACGGCTGCTGAAGGAGATGGAGTGGTACGATAACTAt                                                                                                     >  1:16827/1‑133 (MQ=255)                      gcgcTGCGGCGCAACGGTGATGTTTTCAGAAGTAACGGAAGTGCGTGACGCGATCCATCTGCTGACACCACGCGCAGTGAACGAAGAGGTCGGCAAACGGCTCCTGAAGGGGATAGAGTGGTACGATAACTAt                                                                                                     >  1:204254/1‑133 (MQ=255)                      gcgcTGCGGCGCAACGGGGATGTTTTCAGAAGTAACGGAAGTGCGTGACGCGATCCATCTGCTGACACCACGCGCAGTGAACGAAGAGGTCGGCAAACGGCTGCTGAAGGAGATGGAGTGGTACGATAACTAt                                                                                                     >  2:534493/1‑133 (MQ=255)                       cgcTGCGGCGCAACGGTGATGTTTTCAGAAGTAACGGAAGTGCGTGACGCGATCCATCTGCTGACACCACGCGCAGTGAACGAAGAGGTCGACAAACGGCAGCTGAAGCAGATGGAGCGGTACGATAACTAtc                                                                                                    >  2:217581/1‑133 (MQ=255)                        gcTGCGGCGCAACGGTGATGTTTTCAGAAGTAACGGAAGTGCGTGACGCGATCCATCTGCTGACACCACGCGCAGTGAACGAAGAGGTCGGCAAACGGCTCCTGAAGGAGATGGAGTTGTACGATAACTAtct                                                                                                   >  2:234336/1‑133 (MQ=255)                         cTGCGGCGCAACGGTGATGTTTTCAGAAGTAACGGAAGTGCGTGACGCGATCCATCTGCTGACACCACGCGCAGTGAACGAAGAGGTCGGCAAACGGCTGCTTAAGGAGATGGACTGATACGATAACTAtctc                                                                                                  >  1:274566/1‑133 (MQ=255)                         cTGCGGCGCAACGGTGATGTTTTCAGAAGTAACGGAAGTGCGTGACGCGATCCATCTGCTGACACCACGCGCAGTGAACGAAGAGGTCGGCAAACGGCTGCTGAAGGAGATGGAGTGGTACGATAACTAtctc                                                                                                  >  2:491660/1‑133 (MQ=255)                              gcgcAACGGTGATGTTTTCAGAAGTAACGGAAGTGCGTGACGCGATCCATCTGCTGACACCACGCGCAGTGAACGAAGAGGTCGGCAAACGGCTGCTGAAGGAGATGGAGTGGTACGATAACTATCTCAatat                                                                                             >  2:52834/1‑133 (MQ=255)                                   aCGGTGATTTTTTCATAAGTAACGGAAGTGCGTGACGCGATCCATCTGCTGACACCACGCGCAGTGAACGAAGAGGTCGGCAAACGGCTGCTGAAGGAGATGGAGTGGTACGATAACTATCTCAATATGGGaa                                                                                        <  1:304827/133‑1 (MQ=255)                                             tttCAGAAGTAACGGAAGTGCGTGACGCGATCCATCTGCTGACACCACGCGCAGTGAACGAAGAGGTCGGCAAACGGCTGCTGAAGGAGATGGAGTGGTACGATAACTATCTCAATATGGGAAAAaccgaccg                                                                              <  2:40197/133‑1 (MQ=255)                                                    aGTAACGGTAGTGCGAGACGCGCTCCATCTCCTGACACCACGCGCAGTGAACGAAGAGGTCGGCAAACGGCTGCTGAAGGAGATGGAGTGGTACGATAACTATCTCAATATGGGAAAAACCGACCGCAGCGcc                                                                       <  1:169361/133‑1 (MQ=255)                                                              gTGCGTGACGGGATCTATCTGCTGCCACCACGCGCAGTGAATGAAGAGGTCGGCAAACGGCTGCTGAAGGAGATGGAGTGGTACGATAACTATCTCAATATGGGAAAAACCGACCGCAGCGCCAACCCTTCGc                                                             <  2:204254/133‑1 (MQ=255)                                                                gCGTGACGCGGTCCACCTGCTGACCCCACGCGCAGTGAACGTAGAGGTCGGCAAACGGCTGCTGAAGGAGATGGAGTGGTACGATAACTATCTCAATATGGGAAAAACCGACCGCAGCGCCAACCCTTCGCCg                                                           <  2:195308/133‑1 (MQ=255)                                                                gCGTGACGCGATCCATCTGCTGACACCACGCGCAGTGAACGAAGAGGTCGGCAAACGGCTGCTGAAGGAGATGGAGTGGTACGATAACTATCTCAATATGGGAAAAACCGACCGCAGCGCCAACCCTTCGCCg                                                           >  1:318074/1‑133 (MQ=255)                                                                 cGTGACGCGATCCATCTGCTGACACCACGCGCAGTGAACGAAGAGGTCGGCAAACGGCTGCTGAAGGAGATGGAGTGGTACGATAACTATCTCAATATGGGAAAAACCGACCGCAGCGCCAACCCTTCGCCgg                                                          >  1:403678/1‑133 (MQ=255)                                                                   tGACGCGATCCATCTGCTGACACCACGCGCAGTGAACGAAGAGGTCGGCAAACGGCTGCTGAAGGAGATGGAGTGGTACGATAACTATCTCAATATGGGAAAAACCGACCGCAGCGCCAACCCTTCGCCGGGc                                                        <  2:318074/133‑1 (MQ=255)                                                                   tGACGCGATCCATCTGCTGACACCACGCGCAGTGAACGAAGAGGTCGGCAAACGGCTGCTGAAGGAGATGGAGTGGTACGATAACTATCTCAATATGGGAAAAACCGACCGCAGCGCCAACCCTTCGCCGGGc                                                        <  2:483673/133‑1 (MQ=255)                                                                              aTCTGCTGACACCACGCGCAGTGAACGAAGAGGTCGGCAAACGGCTGCTGAAGGAGATGGAGTGGTACGATAACTATCTCAATATGGGAAAAACCGACCGCAGCGCCAACCCTTCGCCGGGCAACAAGAAAgg                                             <  1:534493/133‑1 (MQ=255)                                                                                     gACACCACGCGCAGTGAACGAAGAGGTCGGCAAACGGCTGCTGAAGGAGATGGAGTGGTACGATAACTATCTCAATATGGGAAAAACCGACCGCAGCGCCAACCCTTCGCCGGGCAACAAGAAAGGCGGTCTg                                      >  2:538327/1‑133 (MQ=255)                                                                                     gACACCACACGCAGTAAACGAATAGGTCGGCAAACGGCTGCTGAAGGAGATGGAGTGGTACGATAACTATCTCAATATGGGAAAAACCGACCGCAGCGCCAACCCTTCGCCGGGCAACAAGAAAGGCGGTCTg                                      <  1:234336/133‑1 (MQ=255)                                                                                             gcgcAGTGAACGAAGAGGTCGGCAAACGGCTGCTGAAGGAGATGGAGTGGTACGATAACTATCTCAATATGGGAAAAACCGACCGCAGCGCCAACCCTTCGCCGGGCAACCAGAAAGTCGGTCTTGCAAACGt                              >  1:315763/1‑133 (MQ=255)                                                                                             gcgcAGTGAACGAAGAGGTCGGCAAACGGCTGCTGAAGGAGATGGAGTGGTACGATAACTATCTCAATATGGGAAAAACCGACCGCAGCGCCAACCCTTCGCCGGGCAACAAGAAAGGCGGTCTGGCAAACGt                              >  1:497365/1‑133 (MQ=255)                                                                                             gcgcAGTGAACGAAGAGGTCGGCAAACGGCTGCTGAAGGAGATGGAGTGGTACGATAACTATCTCAATATGGGAAAAACCGACCGCAGCGCCAACCCTTCGCCGGGCAACAAGAAAGGCGGTCTGGCAAACGt                              >  1:407184/1‑133 (MQ=255)                                                                                                  gTGAACGAAGAGGTCGGCAAACGGCTGCTGAAGGAGATGGAGTGGTACGATAACTATCTCAATATGGGAAAAACCGACCGCAGCGCCAACCCTTCGCCGGGCAACAAGAAAGGCGGTCTGGCAAACGTGGTag                         >  1:249473/1‑133 (MQ=255)                                                                                                     aaCGAAGAGGTCGGCAAACGGCTGCTGAAGGAGATGGAGTGGTACGATAACTATCTCAATATGGGAAAAACCGACCGCAGCGCCAACCCTTCGCCGGGCAACAAGAAAGGCGGTCTGGCAAACGTGGTAgaga                      <  1:52834/133‑1 (MQ=255)                                                                                                     aaCGAAGAGGTCGGCAAACGGCTGCTGAAGGAGATGGAGTGGTACGATAACTATCTCAATATGGGAAAAACCGACCGCAGCGCCAACCCTTCGCCGGGCAACAAGAAAGGCGGTCTGGCAAACGTGGTAgaga                      >  1:437534/1‑133 (MQ=255)                                                                                                     aaCGAAGAGGTCGGCAAACGGCTGCTGAAGGAGATGGAGTGGTACGATAACTATCTCAATATGGGAAAAACCGACCGCAGCGCCAACCCTTCGCCGGGCAACAAGAAAGGCGGTCTGGCAAACGTGGTAgaga                      >  1:374721/1‑133 (MQ=255)                                                                                                            agGTCGGCAAACGGCTGCTGAAGGAGATGGAGTGGTACGATAACTATCTCAATATGGGAAAAACCGACCGCAGCGCCAACCCTTCGCCGGGCAACAAGAAAGGCGGTCTGGCAAACGTGGTAGAGAAGGCACt               <  2:437534/133‑1 (MQ=255)                                                                                                             ggTCGGCAAACGGCTGCTGAAGGAGATGGAGTGGTACGATAACTATCTCAATATGGGAAAAACCGACCGCAGCGCCAACCCTTCGCCGGGCAACAAGAAAGGCGGTCTGGCAAACGTGGTAGAGAAGGCACTc              >  1:370676/1‑133 (MQ=255)                                                                                                               tCGGCAAACGGCTGCTGAAGGAGATGGAGTGGTACGATAACTATCTCAATATGGGAAAAACCGACCGCAGCGCCAACCCTTCGCCGGGCAACAAGAAAGGCGGTCTGGCAAACGTGGTAGAGAAGGCACTCgg            >  2:272838/1‑133 (MQ=255)                                                                                                               tCGGCAAACGGCTGCTGAAGGAGATGGAGTGGTACGATAACTATCTCAATATGGGAAAAACCGACCGCAGCGCCAACCCTTCGCCGGGCAACAAGAAAGGCGGTCTGGCAAACGTGGTAGAGAAGGCACTCgg            >  1:116105/1‑133 (MQ=255)                                                                                                                 ggCAAACGGCTGCTGAAGGAGATGGAGTGGTACGATAACTATCTCAATATGGGAAAAACCGACCGCAGCGCCAACCCTTCGCCGGGCAACAAGAAAGGCGGTCTGGCAAACGTGGTAGAGAAGGCACTCGGCt          <  1:570525/133‑1 (MQ=255)                                                                                                                  gCAAACGGCTGCTGAAGGAGATGGAGTGGTACGATAACTATCTCAATATGGGAAAAACCGACCGCAGCGCCAACCCTTCGCCGGGCAACAAGAAAGGCGGTCTGGCAAACGTGGTAGAGAAGGCACTCGGCTc         <  2:370676/133‑1 (MQ=255)                                                                                                                         gctgctGAAGGAGATGGAGTGGTACGATAACTATCTCAATATGGGAAAAACCGACCGCAGCGCCAACCCTTCGCCGGGCAACAAGAAAGGCGGTCTGGCAAACGTGGTAGAGAAGGCACTCGGCTCCATTGCt  <  2:249473/133‑1 (MQ=255)                                                                                                                         gctgctGAAGGAGATGGAGTGGTACGATAACTATCTCAATATGGGAAAAACCGACCGCAGCGCCAACCCTTCGCCGGGCAACAAGAAAGGCGGTCTGGCAAACGTGGTAGAGAAGGCACTCGGCTCCATTGCt  <  1:272838/133‑1 (MQ=255)                                                                                                                                |                                                                                                                              CTATGCGTCTGATCTACTGGTGCGCTGCGGCGCAACGGTGATGTTTTCAGAAGTAACGGAAGTGCGTGACGCGATCCATCTGCTGACACCACGCGCAGTGAACGAAGAGGTCGGCAAACGGCTGCTGGAGGAGATGGAGTGGTACGATAACTATCTCAATATGGGAAAAACCGACCGCAGCGCCAACCCTTCGCCGGGCAACAAGAAAGGCGGTCTGGCAAACGTGGTAGAGAAGGCACTCGGCTCCATTGCT  >  NC_000913/3276193‑3276445

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑