Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,335,900	T→C	I199V (ATT→GTT)	murA ←	UDP‑N‑acetylglucosamine 1‑carboxyvinyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,335,900	0	T	C	100.0%	188.5 / NA	52	I199V (ATT→GTT)	murA	UDP‑N‑acetylglucosamine 1‑carboxyvinyltransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (26/26);  total (26/26)

GTACCGGTTTCGATACGATCCGGCAGAACGCGATAGACACCGCCGCCTAAACGTTCCACACCTTCGATGACGATACGATCGGTGCCCTGACCGCTAATTTTCGCACCCAGCGTAATCAGGAAGTTCGCGGTATCGACGATTTCCGGTTCACGCGCTGCGTTTTCAATAATCGTGGTGCCTTCCGCCAGGGTTGCAGCACACATGATGGTCACCGTTGCGCCAACGCTGACTTTATCCATCACG  >  NC_000913/3335785‑3336027                                                                                                                    |                                                                                                                                gTACCGGTTTCGATACGATCCGGCAGAACGCGATAGACACCGCCGCCTAAACGTTCCACACCTTCGATGACGATACG‑TCGGTGCCCTGACCGCTAATTTTCGCACCCAGCGTAACCAGGAAGTTCGCGGTATc                                                                                                               >  2:89928/1‑133 (MQ=255)  tACCGGTTTCGATACGATCCGGCAGAACGCGATAGACACCGCCGCCTAAACGTTCCACACCTTCGATGACGATACGATCGGTGCCCTGACCGCTAATTTTCGCACCCAGCGTAACCAGGAAGTTCGCGGTATc                                                                                                               >  1:470754/1‑133 (MQ=255)    ccGGTTTCGATACGATCCGGCAGAACGCGATAGACACCGCCGCCTAAACGTTCCACACCTTCGATGACGATACGATCGGTGCCCTGACCGCTAATTTTCGCACCCAGCGTAACCAGGAAGTTCGCGGTATcga                                                                                                             >  2:547206/1‑133 (MQ=255)       gTTTCGATACGATCCGGCAGAACGCGATAGACACCGCCGCCTAAACGTTCCACACCTTCGATGACGATACGATCGGTGCCCTGACCGCTAATTTTCGCACCCAGCGTAACCAGGAAGTTCGCGGTATcgacga                                                                                                          >  1:385706/1‑133 (MQ=255)              tACGATCCGTTAGAACGCGATAGACATCTCCGCCTAAACGTTCCACACCTTCGATGACGATACGATCGGTGCCCTGACCGCTAATTTTCGCACCCAGCGTAACCAGGAAGTTCGCGGTATCGACGATTTCCgg                                                                                                   <  2:309332/133‑1 (MQ=255)                  aTCCGGCAGAACGCGATAGACACCGCCGCCTAAACGTTCCACACCTTCGATGACGATACGATCGGTGCCCTGACCGCTAATTTTCGCACCCAGCGTAACCAGGAAGTTCGCGGTATTGACGATTTCCGGTTCa                                                                                               >  1:523405/1‑133 (MQ=255)                           aaCGCGATAGACACCGCCGCCTAAACGTTCCACACCTTCGATGACGATACGATCGGTGCCCTGACCGCTAATTTTCGCACCCAGCGTAACCAGGAAGTTCGCGGTATCGACGATTTCCGGTTCACGCGCTGCg                                                                                      >  2:384358/1‑133 (MQ=255)                           aaCGCGATAGACACCGCCGCCTAAACGTTCCACACCTTCGATGACGATACGATCGGTGCCCTGACCGCTAATTTTCGCACCCAGCGTAACCAGGAAGTTCGCGGGAACTACGATTTCGGGTTCACGCGCTGCg                                                                                      >  2:276701/1‑133 (MQ=255)                             cgcgATAGACACCGCCGCCTAAACGTTCCACACCTTCGATGACGATACGATCGGTGCCCTGACCGCTAATTTTCGCACCCAGCGTAACCAGGAAGTTCGCGGTATCGACGATTTCCGGTTCACGCGCTGCGtt                                                                                    >  2:23165/1‑133 (MQ=255)                             cgcgATAGACACCGCCGCCTAAACGTTCCACACCTTCGATGACGATACGATCGGTGCCCTGACCGCTAATTTTCGCACCCAGCGTAACCAGGAAGTTCGCGGTATCGACGATTTCCGGTTCACGCGCTGCGtt                                                                                    >  1:66309/1‑133 (MQ=255)                             cgcgATAGACACCGCCGCCTAAACGTTCCACACCTTCGATGACGATACGATCGGTGCCCTGACCGCTAATTTTCGCACCCAGCGTAACCAGGAAGTTCGCGGTATCGACGATTTCCGGTTCACGCGCTGCGtt                                                                                    <  1:384358/133‑1 (MQ=255)                                 aTAGACACCGCCGCCTAAACGTTCCACACCTTCGATGACGATACGATCGGTGCCCTGACCGCTAATTTTCGCACCCAGCGTAACCAGGAAGTTCGCGGTATCGACGATTTCCGGTTCACGCGCTGCGTTTTCa                                                                                >  2:46577/1‑133 (MQ=255)                                          gccgccTAAACGTTCCACACCTTCGATGACGATACGATCGGTGCCCTGACCGCTAATTTTCGCACCCAGCGTAACCAGGAAGTTCGCGGTATCGACGATTTCCGGTTCACGCGCTGCGTTTTCAATAATCgtg                                                                       >  1:10574/1‑133 (MQ=255)                                          gccgccTAAACGTTCCACACCTTCGATGACGATACGATCGGTGCCCTGACCGCTAATTTTCGCACCCAGCGTAACCAGGAAGTTCGCGGTATCGACGATTTCCGGTTCACGCGCTGCGTTTTCAATAATCgtg                                                                       >  1:592426/1‑133 (MQ=255)                                               cTAAACGTTCCACACCTTCGATGACGATACGATCGGTGCCCTGACCGCTAATTTTCGCACCCAGCGTAACCAGGAAGTTCGCGGTATCGACGATTTCCGGTTCACGCGCTGCGTTTTCAATAATCGTGGTGcc                                                                  <  2:624479/133‑1 (MQ=255)                                                      ttCCACACCTTCGATGACGATACGATCGGTGCCCTGACCGCTAATTTTCGCACCCAGCGTAACCAGGAAGTTCGCGGTATCGACGATTTCCGGTTCACGCGCTGCGTTTTCAATAATCGTGGTGCCTTCCGcc                                                           >  1:125336/1‑133 (MQ=255)                                                        ccACATCTTCGATGACGATACGATCGGTGCCCTGACCGCTAATTTTCGCACCCAGCGTAACCAGGAAGTTCGCGGTATCGACGATTTCCGGTTCACGCGCTGCGTTTTCAATAATCGTGGTGCCTTCCGCCAg                                                         >  1:142759/1‑133 (MQ=255)                                                         cacaCCTTCGATGACGATACGATCGGTGCCCTGACCGCTAATTTTCGCACCCAGCGTAACCAGGAAGTTCGCGGTATCGACGATTTCCGGTTCACGCGCTGCGTTTTCAATAATCGTGGTGCCTTCCGCCAgg                                                        >  2:612515/1‑133 (MQ=255)                                                         cacaCCTTCGATGACGATACGATCGGTGCCCTGACCGCTAATTTTCGCACCCAGCGTAACCAGGAAGTTCGCGGTATCGACGATTTCCGGTACACGCGCTACGTTTTCAATAATCGTTGTGCCTTCCGCCAgg                                                        >  2:223921/1‑133 (MQ=255)                                                             ccTTCGATGACGATACGATCGGTGCCCTGACCGCTAATTTTCGCACCCAGCGTAACCAGGAAGTTCGCGGTATCGACGATTTCCGGTTCACGCGCTGCGTTTTCAATAATCGTGGTGCCTTCCGCCAGGGTTg                                                    <  2:125336/133‑1 (MQ=255)                                                             ccTTCGATGACGATACGATCGGTGCCCTGACCGCTAATTTTCGCACCCAGCGTAACCAGGAAGTTCGCGGTATCGACGATTTCCGGTTCACGCGCTGCGTTTTCAATAATCGTGGTGCCTTCCGCCAGGGTTg                                                    <  1:95006/133‑1 (MQ=255)                                                             ccTTCGATGACGATACGATCGGTGCCCTGACCGCTAATTTTCGCACCCAGCGTAACCAGGAAGTTCGCGGTATCGACGATTTCCGGTTCACGCGCTGCGTTTTCAATAATCGTGGTGCCTTCCGCCAGGGTTg                                                    <  2:385706/133‑1 (MQ=255)                                                              cTTCGATGACGATACGATCGGTGCCCTGACCGCTAATTTTCGCACCCAGCGTAACCAGGAAGTTCGCGGTATTGACGATTTCCGGTTCACGCGCTGCGTTTTCAATAATCGTGGTGCCTTCCGCCAGGGTTgc                                                   <  2:523405/133‑1 (MQ=255)                                                               ttCGATGACGATACGATCGGTGCCCTGACCGCTAATTTTCGCACCCAGCGTAACCAGGAAGTTCGCGGTATCGACGATTTCCGGTTCACGCGCTGCGTTTTCAATAATCGTGGTGCCTTCCGCCAGGGTTgca                                                  <  2:142759/133‑1 (MQ=255)                                                                 cGATGACGATACGATCGGTGCCCTGACCGCTAATTTTCGCACCCAGCGTAACCAGGAAGTTCGCGGTATCGACGATTTCCGGTTCACGCGCAGCGTTTTCAATAGTCGGGGTGCCTTTCGCCAGGGTTgcagc                                                >  2:188637/1‑133 (MQ=255)                                                                 cGATGACGATACGATCGGTGCCCTGACCGCTAATTTTAGCACCCAGCGTAACCAGGAAGTTCGCGGTATCGACGATTTCCGGTTCACGCGCTGCGTTTTCAATAATCGTGATGCCTTCCGCCAGGGTTgcagc                                                >  1:438120/1‑133 (MQ=255)                                                                   aTGACGATACGATCGGTGCCCTGACCGCTAATTTTCGCACCCAGCGTAACCAGGAAGTTCGCGGTATCGACGATTTCCGGTTCACGCGCTGCGTTTTCAATAATCGTGGTGCCTTCCGCCAGGGTTGCAGcac                                              >  2:519442/1‑133 (MQ=255)                                                                    tGACGATACGATCGGTGCCCTGACCGCTAATTTTCGCACCCAGCGTAACCAGGAAGTTCGCGGTATCGACGATTTCCGGTTCACGCGCTGCGTTTTCAATAATCGTGGTGCCTTCCGCCAGGGTTGCAGcaca                                             <  1:434396/133‑1 (MQ=255)                                                                      acgatacgatCGGTGCCCTGACCGCTAATTTTCGCACCCAGCGTAACCAGGAAGTTCGCGGTATCGACGATTTCCGGTTCACGCGCTGCGTTTTCAATAATCGTGGTGCCTTCCGCCAGGGTTGCAGcacaca                                           <  1:19479/133‑1 (MQ=255)                                                                        gatacgatCGGTGCCCTGACCGCTAATTTTCGCACCCAGCGTAACCAGGAAGTTCGCGGTATCGACGATTTCCGGTTCACGCGCTGCGTTTTCAATAATCGTGGTGCCTTCCGCCAGGGTTGCAGCACACatg                                         >  2:604446/1‑133 (MQ=255)                                                                        gatacgatCGGTGCCCTGACCGCTAATTTTCGCACCCAGCGTAACCAGGAAGTTCGCGGTATCGACGATTTCCGGTTCACGCGCTGCGTTTTCAATAATCGTGGTGCCTTACGCAAGAGTTGCATCACACatg                                         >  1:181693/1‑133 (MQ=255)                                                                          tacgatCGGTGCCCTGACCGCTAATTTTCGCACCCAGCGTAACCAGGAAGTTCGCGGTATCGACGATTTCCGGTTCACGCGCTGCGTTTTCAATAATCGTGGTGCCTTCCGCCAGGGTTGCAGCACACatgat                                       >  1:104870/1‑133 (MQ=255)                                                                             gatCGGTGCCCTGACCGCTAATTTTCGCACCCAGCGTAACCAGGAAGTTCGCGGTATCGACGATTTCCGGTTCACGCGCTGCGTTTTCAATAATCGTGGTGCCTTCCGCCAGGGTTGCAGCACACATGATGGt                                    <  1:556287/133‑1 (MQ=255)                                                                               tCGGTGCCCTGACCGCTAATTTTCGCACCCAGCGTAACCAGGAAGTTCGCGGTATCGACGATTTCCGGTTCACGCGCTGCGTTTTCAATAATCGTGGTGCCTTCCGCCAGGGTTGCAGCACACATGATGGTCa                                  >  1:28531/1‑133 (MQ=255)                                                                                     cccTGACCGCTAATTTTCGCACCCAGCGTAACCAGGAAGTTCGCGGTATCGACGATTTCCGGTTCACGCGCTGCGTTTTCAATAATCGTGGTGCCTTCCGCCAGGGTTGCAGCACACATGATGGTCACCGTTg                            >  2:480200/1‑133 (MQ=255)                                                                                       cTGACCGCTAATTTTCGCACCCAGCGTAACCAGGAAGTTCGCGGTATCGACGATTTCCGGTTCACGCGCTGCGTTTTCAATAATCGTGGTGCCTTCCGCCAGGGTTGCAGCACACATGATGGTCACCGTTgcg                          <  1:77219/133‑1 (MQ=255)                                                                                        tGACCGCTAATTTTCGCACCCAGCGTAACCAGGAAGTTCGCGGTATCGACGATTTCCGGTTCACGCGCTGCGTTTTCAATAATCGTGGTGCCTTCCGCCAGGGTTGCAGCACACATGATGGTCACCGTTgcgc                         <  2:66309/133‑1 (MQ=255)                                                                                        tGACCGCTAATTTTCGCACCCAGCGTAACCAGGAAGTTCGCGGTATCGACGATTTCCGGTTCACGCGCTGCGTTTTCAATAATCGTGGTGCCTTCCGCCAGGGTTGCAGCACACATGATGGTCACCGTTgcgc                         <  2:470754/133‑1 (MQ=255)                                                                                         gACCGCTAATTTTCGCACCCAGCGTAACCAGGAAGTTCGCGGTATCGACGATTTCCGGTTCACGCGCTGCGTTTTCAATAATCGTGGTGCCTTCCGCCAGGGTTGCAGCACACATGATGGTCACCGTTGCGcc                        >  1:79428/1‑133 (MQ=255)                                                                                         gACCGCTAATTTTCGCACCCAGCGTAACCAGGAAGTTCGCGGTATCGACGATTTCCGGTTCACGCGCTGCGTTTTCAATAATCGTGGTGCCTTCCGCCAGGGTTGCAGCACACATGATGGTCACCGTTGCGcc                        <  1:612515/133‑1 (MQ=255)                                                                                            cGCTAATTATCGCACTCAGCGTCACCAGGAAGTTCGCGGTATCGACGATTTCCGGTTCACGCGCTGCGTTTTCAATAATCGTGGTGCCTTCCGCCAGGGTTGCAGCACACATGATGGTCACCGTTGCGCCAAc                     <  1:276701/133‑1 (MQ=255)                                                                                             gCTAATTTTCGCACCCAGCGTAACCAGGAAGTTCGCGGTATCGACGATTTCCGGTTCACGCGCTGCGTTTTCAATAATCGTGGTGCCTTCCGCCAGGGTTGCAGCACACATGATGGTCACCGTTGCGCCAACg                    <  2:10574/133‑1 (MQ=255)                                                                                             gCTAATTTTCGCACCCAGCGTAACCAGGAAGTTCGCGGTATCGACGATTTCCGGTTCACGCGCTGCGTTTTCAATAATCGTGGTGCCTTCCGCCAGGGTTGCAGCACACATGATGGTCACCGTTGCGCCAACg                    <  2:592426/133‑1 (MQ=255)                                                                                              cTAATTTTCGCACCCAGCGTAACCAGGAAGTTCGCGGTATCGACGATTTCCGGTTCACGCGCTGCGTTTTCAATAATCGTGGTGCCTTCCGCCAGGGTTGCAGCACACATGATGGTCACCGTTGCGCCAACGc                   <  1:404857/133‑1 (MQ=255)                                                                                               tAATTTTCCCACCCACCGCAACTAGGAAGTGCGCGGTATCGACGATTTCCGGTTCACGCGCTGCGTTTTCAATAATCGTGGTGCCTTCCGCCAGGGTTGCAGCACACATGATGGTCACCGTTGCGCCAACGCt                  <  1:188637/133‑1 (MQ=255)                                                                                                      cGCACCCAGCGTAACCAGGAAGTTCGCGGTATCGACGATTTCCGGTTCACGCGCTGCGTTTTCAATAATCGTGGTGCCTTCCGCCAGGGTTGCAGCACACATGATGGTCACCGTTGCGCCAACGCTGACTTTa           <  2:104870/133‑1 (MQ=255)                                                                                                       gCACCCAGCGTAACCAGGAAGTTCGCGGTATCGACGATTTCCGGTTCACGCGCTGCGTTTTCAATAATCGTGATGCCTTCCGCCAGGGTTGCAGCACACATGATGGTCACCGTTGCGCCAACGCTGACTTTAt          <  2:438120/133‑1 (MQ=255)                                                                                                         aCCCAGCGTAACCAGGAAGTTCGCGGTATCGACGATTTCCGGTTCACGCGCTGCGTTTTCAATAATCGTGGTGCCTTCCGCCAGGGTTGCAGCACACATGATGGTCACCGTTGCGCCAACGCTGACTTTATcc        >  2:83400/1‑133 (MQ=255)                                                                                                              gCGTAACCAGGAAGTTCGCGGTATCGACGATTTCCGGTTCACGCGCTGCGTTTTCAATAATCGTGGTGCCTTCCGCCAGGGTTGCAGCACACATGATGGTCACCGTTGCGCCAACGCTGACTTTATCCATCAc   <  1:89928/133‑1 (MQ=255)                                                                                                              gCGTAACCAGGAAGTTCGCGGTATCGACGATTTCCGGTTCACGCGCTGCGTTTTCAATAATCGTGGTGCCTTCCGCCAGGGTTGCAGCACACATGATGGTCACCGTTGCGCCAACGCTGACTTTATCCATCAc   <  1:36612/133‑1 (MQ=255)                                                                                                               cGTAACCAGGAAGTTCGCGGTATCGACGATTTCCGGTTCACGCGCTGCGTTTTCAATAATCGTGGTGCCTTCCGCCAGGGTTGCAGCACACATGATGGTCACCGTTGCGCCAACGCTGACTTTATCCATCACg  <  1:547206/133‑1 (MQ=255)                                                                                                               cGTAACCAGGAAGTTCGCGGTATCGACGATTTCCGGTTCACGCGCTGCGTTTTCAATAATCGTGGTGCCTTCCGCCAGGGTTGCAGCACACATGATGGTCACCGTTGCGCCAACGCTGACTTTATCCATCACg  <  1:23165/133‑1 (MQ=255)                                                                                                                    |                                                                                                                                GTACCGGTTTCGATACGATCCGGCAGAACGCGATAGACACCGCCGCCTAAACGTTCCACACCTTCGATGACGATACGATCGGTGCCCTGACCGCTAATTTTCGCACCCAGCGTAATCAGGAAGTTCGCGGTATCGACGATTTCCGGTTCACGCGCTGCGTTTTCAATAATCGTGGTGCCTTCCGCCAGGGTTGCAGCACACATGATGGTCACCGTTGCGCCAACGCTGACTTTATCCATCACG  >  NC_000913/3335785‑3336027

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑