Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,380,327	A→G	D46G (GAC→GGC)	yhcB →	conserved protein YhcB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,380,327	0	A	G	100.0%	138.2 / NA	39	D46G (GAC→GGC)	yhcB	conserved protein YhcB
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (22/17);  total (22/17)

GGGAATATGCGCTAATTGGGTTAGTCGTCGGCATCATTATTGGTGCTGTGGCCATGCGTTTTGGTAATCGTAAACTACGCCAGCAACAGGCGTTGCAGTACGAACTGGAAAAGAATAAAGCTGAACTGGACGAGTATCGCGAAGAGCTGGTTAGCCACTTTGCCCGCAGCGCGGAATTACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAA  >  NC_000913/3380198‑3380451                                                                                                                                  |                                                                                                                             gggAATATGCGCTAATTGGGTTAGTCGTCGGCATCATTATTGGTGCTGTGGCCATGCGTTTTGGTAATCGTAAACTACGCCAGCAACAGGCGTTGCAGTACGAACTGGAAAAGAATAAAGCTGAACTGGGCGa                                                                                                                           >  2:578534/1‑133 (MQ=255) gggAATATGCGCTAATTGGGTTAGTCGTCGGCATCATTATTGGTGCTGTGGCCATGCGTTTTGGTAATCGTAAACTACGCCAGCAACAGGCGTTGCAGTACGAACTGGAAAAGAATAAAGCTGAACTGGGCGa                                                                                                                           <  1:39897/133‑1 (MQ=255)        tGCGCTAATTGGGTTAGTCGTCGGCATCATTATTGGTGCTGTGGCCATGCGTTTTGGTAATCGTAAACTACGCCAGCAACAGGCGTTGCAGTACGAACTGGAAAAGAATAAAGCTGAACTGGGCGAGTATcgc                                                                                                                    >  1:347068/1‑133 (MQ=255)        tGCGCTAATTGGGTTAGTCGTCGGCATCATTATTGGTGCTGTGGCCATGCGTTTTGGTAATCGTAAACTACGCCAGCAACAGGCGTTGCAGTACGAACTGGAAAAGAATAAAGCTGAACTGGGCGAGTATcgc                                                                                                                    >  1:378436/1‑133 (MQ=255)              aaTTGGGTTAGTCGTCGGCATCATTATTGGTGCTGTGGCCATGCGTTTTGGTAATCGTAAACTACGCCAGCAACAGGCGTTGCAGTACGAACTGGAAAAGAATAAAGCTGAACTGGGCGAGTATCGCGAagag                                                                                                              <  1:578534/133‑1 (MQ=255)                         tcgtcgGCATCATTATTGGTGCTGTGGCCATGCGTTTTGGTAATCGTAAACTACGCCAGCAACAGGCGTTGCAGTACGAACTGGAAAAGAATAAAGCTGAACTGGGCGAGTATCGCGAAGAGCTGGTTAGCCa                                                                                                   >  2:116026/1‑133 (MQ=255)                             cgGCATCATTATTGGTGCTGTGGCCATGCGTTTTGGTAATCGTAAACTACGCCAGCAACAGGCGTTGCAGTACGAACTGGAAAAGAATAAAGCTGAACTGGGCGAGTATCGCGAAGAGCTGGTTAGCCACttt                                                                                               >  2:318110/1‑133 (MQ=255)                             cgGCATCATTATTGGTGCTGTGGCCATGCGTTTTGGTAATCGTAAACTACGCCAGCAACAGGCGTTGCAGTACGAACTGGAAAAGAATAAAGCTGAACTGAGCGGGTATCGCGAAGACCTGGTTAGCCACttt                                                                                               >  2:192835/1‑133 (MQ=255)                                     ttattGGTGCTGTGGCCATGCGTTTTGGTAATCGTAAACTACGCCAGCAACAGGCGTTGCAGTACGAACTGGAAAAGAATAAAGCTGAACTGGGCGAGTATCGCGAAGAGCTGGTTAGCCACTTTGCCCGCAg                                                                                       <  2:378436/133‑1 (MQ=255)                                       attGGTGCTGTGGCCATGCGTTTTTGTATTCGTAAACTACGCCAGCAACAGGCGTTGCAGTACGAACTGGAAAAGAATAAAGCTGAACTGGGCGAGTATCGCGAAGAGCTGGTTAGCCACTTTGCCCGCAgcg                                                                                     <  2:214149/133‑1 (MQ=255)                                             gCTGTGGCCATGCGTTTTGGTAATCGTAAACTACGCCAGCAACAGGCGTTGCAGTACGAACTGGAAAAGAATAAAGCTGAACTGGGCGAGTATCGCGAAGAGCTGGTTAGCCACTTTGCCCGCAGCGCGGAAt                                                                               >  1:14729/1‑133 (MQ=255)                                             gCTGTGGCCATGCGTTTTGGTAATCGTAAACTACGCCAGCAACAGGCGTTGCAGTACGAACTGGAAAAGAATAAAGCTGAACTGGGCGAGTATCGCGAAGAGCTGGTTAGCCACTTTGCCCGCAGCGCGGAAt                                                                               >  2:617499/1‑133 (MQ=255)                                             gCTGTGGCCATGCGTCTTCGTATTCGTAAACTACGCCAGCAACAGGCGTTGCAGTACGAACTGGAAAAGAATAAAGCTGAACTGGGCGAGTATCGCGAAGAGCTGGTTAGCCACTTTGCCCGCAGCGCGGAAt                                                                               <  1:206686/133‑1 (MQ=255)                                              cTGTGGCCATGCGTTTTGGTAATCGTAAACTACGCCAGCAACAGGCGTTGCAGTACGAACTGGAAAAGAATAAAGCTGAACTGGGCGAGTATCGCGAAGAGCTGGTTAGCCACTTTGCCCGCAGCGCGGAAtt                                                                              >  1:565852/1‑133 (MQ=255)                                               tgtgGCCATGCGTTTTGGTAATCGTAAACTACGCCAGCAACAGGCGTTGCAGTACGAACTGGAAAAGAATAAAGCTGAACTGGGCGAGTATCGCGAAGAGCTGGTTAGCCACTTTGCCCGCAGCGCGGAATTa                                                                             <  2:358178/133‑1 (MQ=255)                                                gtgGCCATGCGTTTTGGTAATCGTAAACTACGCCAGCAACAGGCGTTGCAGTACGAACTGGAAAAGAATAAAGCTGAACTGGGCGAGTATCGCGAAGAGCTGGTTAGCCACTTTGCCCGCAGCGCGGAAtt                                                                              >  2:426493/1‑131 (MQ=255)                                                gtgGCCATGCGTTTTGGTAATCGTAAACTACGCCAGCAACAGGCGTTGCAGTACGAACTGGAAAAGAATAAAGCTGAACTGGGCGAGTATCGCGAAGAGCTGGTTAGCCACTTTGCCCGCAGCGCGGAAtt                                                                              <  1:426493/131‑1 (MQ=255)                                                gtgGCCATGCGTTTTGGTAATCGTAAACTACGCCAGCAACAGGCGTTGCAGTACGAACTGGAAAAGAATAAAGCTGAACTGGGCGAGTATCGCGAAGAGCTGGTTAGCCACTTTGCCCGCAGCGCGGAATTAc                                                                            >  2:47426/1‑133 (MQ=255)                                                   accATGCGTTTTGGTAATCGTAAACTACGCCAGCAACAGGCGTTGCAGTACGAACTGGAAAAGAATAAAGCTGAACTGGGCGAGTATCGCGAAGAGCTGGTTAGCCACTTTGCCCGCAGCGCGGAATTACTgg                                                                         >  1:105825/2‑133 (MQ=255)                                                      aTGCGTTTTGGTAATCGTAAACTACGCCAGCAACAGGCGTTGCAGTACGAACTGGAAAAGAATAAAGCTGAACTGGGCGAGTATCGCGAAGTGCTGGTTATCCATTTTGCCCGCAGCTCGGAATTACTGGATa                                                                      >  2:167848/1‑133 (MQ=255)                                                      aTGCGTTTTGGTAATCGTAAACTACGCCAGCAACAGGCGTTGCAGTACGAACTGGAAAAGAATAAAGCTGAACTGGGCGAGTATCGCGAAGAGCTGGTTAGCCACTTTGCCCGCAGCGCGGAATTACTGGATa                                                                      >  1:452730/1‑133 (MQ=255)                                                             ttGGTAATCGTAAACTACGCCAGCAACAGGCGTTGCAGTACGAACTGGAAAAGAATAAAGCTGAACTGGGCGAGTATCGCGAAGAGCTGGTTAGCCACTTTGCCCGCAGCGCGGAATTACTGGATACCATGgc                                                               >  1:589497/1‑133 (MQ=255)                                                             ttGGTAATCGTAAACTACGCCAGCAACAGGCGTTGCAGTACGAACTGGAAAAGAATAAAGCTGAACTGGGCGAGTATCGCGAAGAGCTGGTTAGCCACTTTGCCCGCAGCGCGGAATTACTGGATACCATGgc                                                               >  1:24933/1‑133 (MQ=255)                                                                gTAATCGTCAACTACGCCTGCAGCAGGCGTCGCAGTACGAACTGGAAAAGAATAAAGCTGAACTGGGCGAGTATCGCGAAGAGCTGGTTAGCCACTTTGCCCGCAGCGCGGAATTACTGGATACCATGGCGCa                                                            <  1:271639/133‑1 (MQ=255)                                                                   aTCGTAAACTACGCCAGCAACAGGCGTTGCAGTACGAACTGGAAAAGAATAAAGCTGAACTGGGCGAGTATCGCGAAAAGCTGGTTAGCCACTTTGCCCGCAGCGCGGAATTACTGGATACCATGGCGCACGa                                                         <  2:347068/133‑1 (MQ=255)                                                                              cGCCAGCAACAGGCGTTGCAGTACGAACTGGAAAAGAATAAAGCTGAACTGGGCGAGTATCGCGAAGAGCTGGTTAGCCACTTTGCCCGCAGCGCGGAATTACTGGATACCATGGCGCACGACTATCGCCAGc                                              <  2:105825/133‑1 (MQ=255)                                                                              cGCCAGCAACAGGCGTTGCAGTACGAACTGGAAAAGAATAAAGCTGAACTGGGCGAGTATCGCGAAGAGCTGGTTAGCCACTTTGCCCGCAGCGCGGAATTACTGGATACCATGGCGCACGACTATCGCCAGc                                              <  1:617499/133‑1 (MQ=255)                                                                                   gCAACAGGCGTTGCAGTACGAACTGGAAAAGAATAAAGCTGAACTGGGCGAGTATCGCGAAGAGCTGGTTAGCCACTTTGCCCGCAGCGCGGAATTACTGGATACCATGGCGCACGACTATCGCCAGCTGTAt                                         <  2:14729/133‑1 (MQ=255)                                                                                        aGGCGTTGCAGTACGAACTGGAAAAGAATAAAGCTGAACTGGGCGAGTATCGCGAAGAGCTGGTTAGCCACTTTGCCCGCAGCGCGGAATTACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGca                                    <  1:47426/133‑1 (MQ=255)                                                                                              tGCAGTACGAACTGGAAAAGAATAAAGCTGAACTGGGCGAGTATCGCGAAGAGCTGGTTAGCCACTTTGCCCGCAGCGCGGAATTACTGGATACCATGGCGCACGACTATAGCCGGCAGTATCATCACATGGc                              >  1:199199/1‑133 (MQ=255)                                                                                                     cGAACTGGAAAAGAATAAAGCTGAACTGGGCGAGTATCGCGAAGAGCTGGTTAGCCACTTTGCCCGCAGCGCGGAATTACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGc                       <  2:452730/133‑1 (MQ=255)                                                                                                        aCTGGAAAAGAATAAAGCTGAACTGGGCGAGTATCGCGAAGAGCTGGTTAGCCACTTTGCCCGCAGCGCGGAATTACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTcc                    >  2:455031/1‑133 (MQ=255)                                                                                                          tGGAAAAGAATAAAGCTGAACTGGGCGAGTATCGCGAAGAGCTGGTTAGCCACTTTGCCCGCAGCGCGGAATTACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCcag                  >  1:494273/1‑133 (MQ=255)                                                                                                          tGGAAAAGAATAAAGCTGAACTGGGCGAGTATCGCGAAGAGCTGGTTAGCCACTTTGCCCGCAGCGCGGAATTACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCcag                  >  1:594870/1‑133 (MQ=255)                                                                                                              aaaGAATAAAGCTGAACTGGGCGAGTATCGCGAAGAGCTGGTTAGCCACTTTGCCCGCAGCGCGGAATTACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCagcagc              <  2:24933/133‑1 (MQ=255)                                                                                                                aGAATAAAGCTGAACTGGGCGAGTATCGCGAAGAGCTGGTTAGCCACTTTGCCCGCAGCGCGGAATTACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCct            <  2:494273/133‑1 (MQ=255)                                                                                                                  aaTAAAGCTGAACTGGGCGAGTATCGCGAAGAGCTGGTTAGCCACTTTGCCCGCAGCGCGGAATTACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCctgc          >  1:494397/1‑133 (MQ=255)                                                                                                                      aaGCTGAACTGGGCGAGTATCGCGAAGAGCTGGTTAGCCACTTTGCCCGCAGCGCGGAATTACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGcc      >  2:508999/1‑133 (MQ=255)                                                                                                                          tGAACTGGGCGAGTATCGCGAAGAGCTGGTTAGCCACTTTGCCCGCAGCGCGGAATTACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGaa  <  2:17580/133‑1 (MQ=255)                                                                                                                                  |                                                                                                                             GGGAATATGCGCTAATTGGGTTAGTCGTCGGCATCATTATTGGTGCTGTGGCCATGCGTTTTGGTAATCGTAAACTACGCCAGCAACAGGCGTTGCAGTACGAACTGGAAAAGAATAAAGCTGAACTGGACGAGTATCGCGAAGAGCTGGTTAGCCACTTTGCCCGCAGCGCGGAATTACTGGATACCATGGCGCACGACTATCGCCAGCTGTATCAGCACATGGCAAAAAGCTCCAGCAGCCTGCTGCCGGAA  >  NC_000913/3380198‑3380451

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑