Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,388,726 A→G V134A (GTT→GCT)  aaeA ← aromatic carboxylic acid efflux pump membrane fusion protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,388,7260AG97.9% 168.5 / NA 48V134A (GTT→GCT) aaeAaromatic carboxylic acid efflux pump membrane fusion protein
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base G (19/28);  total (20/28)
Fisher's exact test for biased strand distribution p-value = 4.17e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TAAACTCACCGGTATAGACGTTGAGGTTGGTCACCCAGCCATCTGCTGGCGCGCGGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAACCGTTTGTAGTACGTTGTTGGCCTGGTCGATCTCTTCGCGAGACATCGCCTGCACACCGAGACGGTTACGACGTCCGGCCTCCTGGCGTTTCTCCTGTGCCAGTACCTGATAATAAGCAACATCGGC  >  NC_000913/3388601‑3388853
                                                                                                                             |                                                                                                                               
taaACTCACCGGTATAGACGTTGAGGTTGGTCACCCAGCCATCTGCTGGCGCGCGGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAGCCGTTTg                                                                                                                          <  1:119807/133‑1 (MQ=255)
     tCACCGGTATAGACGTTGAGGTTGGTCACCCAGCCATCTGCTGGCGCGCGGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAGCCGTTTgtagta                                                                                                                     >  1:335182/1‑133 (MQ=255)
       aCCGGTATAGACGTTGAGGTTGGTCACCCAGCCATCTGCTGGCGCGCGGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAGCCGTTTGTAGTACg                                                                                                                   >  2:11135/1‑133 (MQ=255)
          ggTATAGATGTTGAGCTTTGTCCCCCAGCCATCTGCTGGCGCGCGGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAGCCGTTTGTAGTACgttg                                                                                                                <  1:240820/133‑1 (MQ=255)
                 aCGTTGAGGTTGGTCACCCAGCCATCTGCTGGCGCGCGGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGAGGCAGAGCGGTTGGTGGTACGTGGTTGGCCt                                                                                                         >  1:179884/1‑133 (MQ=255)
                   gTTGAGGTTGGTCACCCAGCCATCTGCTGGCGCGCGGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAGCCGTTTGTAGTACGTTGTTGGCCTgg                                                                                                       >  2:605770/1‑133 (MQ=255)
                   gTTGAGGTTGGTCACCCAGCCATCTGCTGGCGCGCGGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAGCCGTTTGTAGTACGTTGTTGGCCTgg                                                                                                       >  2:3001/1‑133 (MQ=255)
                    ttGAGGTTGGTCACCCAGCCATCTGCTGGCGCGCGGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAGCCGTTTGTAGTACGTTGTTGGCCTGGt                                                                                                      <  1:3001/133‑1 (MQ=255)
                     tGAGGTTGGTCACCCAGCCATCTGCTGGCGCGCGGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCCAACTGATGCAGAACCGATTGTAGTAGGTTGTTGGCCTGGTc                                                                                                     >  2:240820/1‑133 (MQ=255)
                      gAGGTTGGTCACCCAGCCATCTGCTGGCGCGCGGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTGACTGATGCAGAGGCGTGTGTAGTACGTTGTGGGCCTGGTCg                                                                                                    >  2:180045/1‑133 (MQ=255)
                        ggTTGGTCACCCAGCCATCTGCTGGCGCGCGGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAGCCGTTTGTAGTACGTTGTTGGCCTGGTCGAt                                                                                                  <  1:380914/133‑1 (MQ=255)
                             gTCACCCAGCCATCTGCTGGCGCGCGGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATACAGAGCCGATTGGAGTGCGTTGTTGGCCTGTTCGATCTCtt                                                                                             >  2:178005/1‑133 (MQ=255)
                                aCCCAGCCATCTGTTAGCACGCTGATCACCATGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAGCCGTTTGTAGTACGTTGTTGGCCTGGTCGATCTCTTcgc                                                                                          <  1:275426/133‑1 (MQ=255)
                                aCCCAGCCATCTGCTGGCGCGCGGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAGCCGTTTGTAGTACGTTGTTGGCCTGGTCGATCTCTTcgc                                                                                          <  1:605770/133‑1 (MQ=255)
                                     gCCATCTGCTGGCGCGCGGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAGCCGTTTGTAGTACGTTGTTGGCCTGGTCGATCTCTTCGCGAGAc                                                                                     <  2:462489/133‑1 (MQ=255)
                                     gCCATCTGCTGGCGCGCGGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAGCCGTTTGTAGTACGTTGTTGGCCTGGTCGATCTCTTCGCGAGAc                                                                                     <  1:39852/133‑1 (MQ=255)
                                     gCCATCTGCTGGCGCGCGGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAGCCGTTTGTAGTACGTTGTTGGCCTGGTCGATCTCTTCGCGAGAc                                                                                     <  2:159252/133‑1 (MQ=255)
                                           tgctgGCGCGCGGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAGCCGTTTGTAGTACGTTGTTGGCCTGGTCGATCTCTTCGCGAGACATCGcc                                                                               >  2:581150/1‑133 (MQ=255)
                                            gctgGCGCGCGGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAGCCGTTTGTAGTACGTTGTTGGCCTGGTCGATCTCTTCGCGAGAc                                                                                     >  2:432814/1‑126 (MQ=255)
                                            gctgGCGCGCGGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAGCCGTTTGTAGTACGTTGTTGGCCTGGTCGATCTCTTCGCGAGAc                                                                                     <  1:432814/126‑1 (MQ=255)
                                               ggCGCGCGGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAGCCGTTTGTAGTACGTTGTTGGCCTGGTCGATCTCTTCGCGAGACATCGCCTGca                                                                           <  1:581150/133‑1 (MQ=255)
                                                gcgcgcGGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAGCCGTTTGTAGTACGTTGTTGGCCTGGTCGATCTCTTGGCGAGACATGGCCTGcac                                                                          >  1:249211/1‑133 (MQ=255)
                                                     cgGATCACCGTGCGTACACGATCCAGTTTTACCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAGCCGTTTGTAGTACGTTGTTGGCCTGGTCGATCTCTTCGCGAGACATCGCCTGCACACCga                                                                     <  1:180045/133‑1 (MQ=255)
                                                      ggATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAGCCGTTTGTAGTACGTTGTTGGCCTGGTCGATCTCTTCGCGAGACATCGCCTGCACACCgag                                                                    <  1:11662/133‑1 (MQ=255)
                                                       gATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAGCCGTTTGTAGTACGTTGTTGGCCTGGTCGATCTCTTCGCGAGACATCGCCTGCACACCgaga                                                                   <  2:117119/133‑1 (MQ=255)
                                                                 cGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAGCCGTTTGTAGTACGTTGTTGGCCTGGTCGATCTCTTCGCGAGACATCGCCTGCACACCGAGACGGTTacgac                                                         >  2:388541/1‑133 (MQ=255)
                                                                  gTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAGCCGTTTGTAGTACGTTGTTGGCCTGGTCGATCTCTTCGCGAGACATCGCCAGCAGACGGAGACGATTacgacg                                                        >  1:250753/1‑133 (MQ=255)
                                                                    tCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAGCCGTTTGTAGTACGTTGTTGGCCTGGTCGATCTCTTCGCGAGACGTCGCCTGAACAGCGAGACGGTGACGACGTc                                                      >  1:203294/1‑133 (MQ=255)
                                                                    tCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAGCCGTTTGTAGTACGTTGTTGGCCTGGTCGATCTCTTCGCGAGACATCGCCTGCACACCGAGACGGTTACGACGTc                                                      >  2:492586/1‑133 (MQ=255)
                                                                      aaGATCCATTTTTGCTAGCTCGAGGGTCGCGTGCGCTTTCGCTAACTGATGCAGAGCCGTTTGTAGTACGTTGTTGGCCTGGTCGATCTCTTCGCGAGACATCGCCTGCACACCGAGACGGTTACGACGTCCg                                                    <  1:201168/133‑1 (MQ=255)
                                                                             aGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAGCCGTTTGTAGTACGTTGTTGGCCTGGTCGATCTCTTCGCGAGACATCGCCTGCACACCGAGACGGTTACGACGTCCGGcctcct                                             <  2:15204/133‑1 (MQ=255)
                                                                             aGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAGCCGTTTGTAGTACGTTGTTGGCCTGGTCGATCTCTTCGCGAGACATCGCCTGCACACCGAGACGGTTACGACGTCCGGcctcct                                             >  2:67912/1‑133 (MQ=255)
                                                                                tttGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAGCCGTTTGTAGTACGTTGTTGGCCTGGTCGATCTCTTCGCGAGACATCGCCTGCACACCGAGACGGTTACGACGTCCGGCCTCCTGGc                                          >  1:538693/1‑133 (MQ=255)
                                                                                    ccAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAGCCGTTTGTAGTACGTTGTTGGCCTGGTCGATCTCTTCGCGAGACATCGCCTGCACACCGAAACGGTTACGACGTCAGGCCTCCTGGCGttt                                      >  1:258536/1‑133 (MQ=255)
                                                                                        aTCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAGCCGTTTGTAGTACGTTGTTGGCCTGGTCGATCTCTTCGCGAGACATCGCCTGCACACCGAGACGGTTACGACGTCCGGCCTCCTGGCGTTTCTcc                                  <  2:73870/133‑1 (MQ=255)
                                                                                        aTCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAGCCGTTTGTAGTACATTGTTGGCCTGGTCGATCTCTTCGCGAGACATCGCCTGCACACCGAGACGGTTACGACGTCCGGCCTCCTGGCGTTTCTcc                                  <  2:542962/133‑1 (MQ=255)
                                                                                          cgcgGGTCGCCTGCGCTTTCGCTAACTGATGCAGAGCCGTTTGTAGTACGTTGTTGGCCTGGTCGATCTCTTCGCGAGACATCGCCTGCACACCGAGACGGTTACGACGTCCGGCCTCCTGGCGTTTCTCCtg                                <  1:492586/133‑1 (MQ=255)
                                                                                             gggTCGCCTGCGCTTTCGCTAACTGATGCAGAGCCGTTTGTAGTACGTTGTTGGCCTGGTCGATCTCTTCGCGAGACATCGCCTGCACACCGAGACGGTTACGACGTCCGGCCTCCTGGCGTTTCTCCTGTGc                             <  2:516925/133‑1 (MQ=255)
                                                                                             gggTCGCCTGCGCTTTCGATAACTGATGCATAGCCGTTTGTAGTACGTTGTTGGCCTGGTCGATCTCTTCGCGAGACATCGCCTGCACACCGAGACGGTTACGACGTCCGGCCTCCTGGCGTTTCTCCTGTGc                             <  1:178005/133‑1 (MQ=255)
                                                                                                  gCCTGCGCTTTCGCTAACTGATGCAGAGCCGTTTGTAGTACGTTGTTGGCCTGGTCGATCTCTTCGCGAGACATCGCCTGCACACCGAGACGGTTACGACGTCCGGCCTCCTGGCGTTTCTCCTGTGCCAGTa                        >  2:414764/1‑133 (MQ=255)
                                                                                                   ccTGCGCTTTCGCTAGCTGATGCAAAGCCGTTTGTAGTACGCTGTTGGCCTGGTCGATCTCTTCGCGAGACATCGCCTGCACACCGAGACGGTTACGACGTCCGGCCTCCTGGCGTTTCTCCTGTGCCAGTAc                       <  2:258536/133‑1 (MQ=255)
                                                                                                    cTGCGCTTTCACTAACTGATGCAGAGCCTTTTATAGTACGTTGTTGGCCTGGTCGATCTCTTCGCGAGACATCGCCTGCACACCGAGACGGTTACGACGTCCGGCCTCCTGGCGTTTCTCCTGTGCCAGTAcc                      <  2:203294/133‑1 (MQ=255)
                                                                                                         cTTTCGCTAACTGATGCAGAGCCGTTTGTAGTACGTTGTTGGCCTGGTCGATCTCTTCGCGAGACATCGCCTGCACACCGAGACGGTTACGACGTCCGGCCTCCTGGCGTTTCTCCTGTGCCAGTACCTGata                 <  2:503297/133‑1 (MQ=255)
                                                                                                            tCGCTAACTGATGCAGAGCCGTTTGTAGTACGTTGTTGGCCTGGTCGATCTCTTCGCGAGACATCGCCTGCACACCGAGACGGTTACGACGTCCGGCCTCCTGGCGTTTCTCCTGTGCCAGTACCTGataata              <  1:414764/133‑1 (MQ=255)
                                                                                                            tCGCTAACTGATGCAGAGCCGTTTGTAGTACGTTGTTGGCCTGGTCGATCTCTTCGCGAGACATCGCCTGCACACCGAGACGGTTACGACGTCCGGCCTCCTGGCGTTTCTCCCGTGCCAGTACCTGataata              <  1:11135/133‑1 (MQ=255)
                                                                                                                    tGATGCAGCGCCGTTAGTAGTACGTTGTTGGCCTGGTCGATCTCTTCGCGAGACATCGCCTGCACACCGAGACGGTTACGACGTCCGGCCTCCTGGCGTTTCTCCTGTGCCAGTACCTGATAATAAGCAACAt      <  1:260354/133‑1 (MQ=255)
                                                                                                                      aTGCAGAGCCGTTTGTAGTACGTTGTTGGCCTGGTCGATCTCTTCGCGAGACATCGCCTGCACACCGAGACGGTTACGACGTCCGGCCTCCTGGCGTTTCACCTTTGCAAGTACCTGATAATAAGCAACATCg    >  2:260354/1‑133 (MQ=255)
                                                                                                                        gCAGAGCCGTTTGTATTACGTTGTCGGCGTGGTCGATCTCTTCGCGAGACATCGCCTGCACACCGAGACGGTTACGACGTCCGGCCTCCTGGCGTTTCTCCTGTGCCAGTACCTGATAATAAGCAACATCGGc  <  2:249211/133‑1 (MQ=255)
                                                                                                                             |                                                                                                                               
TAAACTCACCGGTATAGACGTTGAGGTTGGTCACCCAGCCATCTGCTGGCGCGCGGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAACCGTTTGTAGTACGTTGTTGGCCTGGTCGATCTCTTCGCGAGACATCGCCTGCACACCGAGACGGTTACGACGTCCGGCCTCCTGGCGTTTCTCCTGTGCCAGTACCTGATAATAAGCAACATCGGC  >  NC_000913/3388601‑3388853

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑