Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,585,536	G→A	Q430* (CAG→TAG)	ggt ←	glutathione hydrolase proenzyme

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,585,536	0	G	A	100.0%	121.1 / NA	36	Q430* (CAG→TAG)	ggt	glutathione hydrolase proenzyme
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (10/26);  total (10/26)

GTCTTTCACCACAATGGTCGGCGACATCGACGACAGCGGGCGTTTGTTCGGCCCGACGGCGTTGGCATCACCGCCCACCAGCCCGTAAACGTTCGGTACGCCCGGTTTGGCGGAGAAATCATCCATCTGGTTATTAAGCAGAATACCGCTCTCGCCCGCGACAATGCCCGTACCGAAGGTGGTGTTCAGCGTATAGGTCACCGCCACCGCGTTACCATCTTTATCCACCACTGAGTAATGGGTAGTTTGATTACTC  >  NC_000913/3585408‑3585663                                                                                                                                 |                                                                                                                                gTCTTTCACCACAATGGTCGGCGACATCGACGACAGCGGGCGTTTGTTCGGCCCGACGGCGTTGGCATCACCTCCCACCAGCCCGTAAACGTTCGGTACGCCCGGTTTGGCGGAGAAATCATCCATCTAGtta                                                                                                                             <  2:8563/133‑1 (MQ=255)  tCTTTCACCACAATGGTCGGCGACATCGACGACAGCGGGCGTTTGTTCGGCCCGACGGCGTTGGCATCACCGCCCACCAGCCCGTAAACGTTCGGTACGCCCGGTTTGGCGGAGAAATCATCCATCTAGttat                                                                                                                            >  2:475139/1‑133 (MQ=255)   cTTTCACCACAATGGTCGGCGACATCGACGACAGCGGGCGTTTGTTCGGCCCGACGGCGTTGGCATCACCGCCCACCAGCCCGTAAACGTTCGGTACGCCCGGTTTGGCGGAGAAATCATCCATCTAGttatt                                                                                                                           >  2:148890/1‑133 (MQ=255)   cTTTCACCACAATGGTCGGCGACATCGACGACAGCGGGCGTTTGTTCGGCCCGACGGCGTTGGCATCACCGCCCACCAGCCCGTAAACGTTCGGTACGCCCGGTTTGGCGGAGAAATCATCCATCTAGttatt                                                                                                                           <  1:474256/133‑1 (MQ=255)    tttCACCACAATGGTCGGCGACATCGACGACAGCGGGCGTTTGTTCGGCCCGACGGCGTTGGCATCACCGCCCACCAGCCCGTAAACGTTCGGTACGCCCGGTTTGGCGGAGAAATCATCCATCTAGttatta                                                                                                                          >  2:341007/1‑133 (MQ=255)     ttCACCACCATGGTCCGCCACACCGACGACAGCGGGCGTTTGTTCGGCCCGACGGCGTTGGCATCACCGCCCACCAGCCCGTAAACGTTCGGTACGCCCGGTTTGGCGGAGAAATCATCCATCTAGTTATTaa                                                                                                                         <  1:306314/133‑1 (MQ=255)           acaATGGTCGGCGACATCGACGACAGCGGGCGTTTGTTCGGCCCGACGGCGTTGGCATCACCGCCCACCAGCCCGTAAACGTTCGGTACGCCCGGTTTGGCGGAGAAATCATCCATCTAGTTATTAAGCAGaa                                                                                                                   <  1:148890/133‑1 (MQ=255)             aaTGGTCGGCGACATCGACGACAGCGGGCGTTTGTTCGGCCCGACGGCGTTGGCATCACCGCCCACCAGCCCGTAAACGTTCGGTACGCCCGGTTTGGCGGAGAAATCATCCATCTAGTTATTAAGCAGAATa                                                                                                                 <  2:516467/133‑1 (MQ=255)             aaTGGTCGGCGACATCGACGACAGCGGGCGTTTGTTCGGCCCGACGGCGTTGGCATCACCGCCCACCAGCCCGTAAACGTTCGGTACGCCCGGTTTGGCGGAGAAATCATCCATCTAGTTATTAAGCAGAATa                                                                                                                 <  2:479550/133‑1 (MQ=255)             aaTGGTCGGCGACATCGACGACAGCGGGC‑‑‑‑GTTCGGCCCGACGGCGTTGGCATCACCGCCCACCAGCCCGTAAACGTTCGGTACGCCCGGTTAGGCGGAGAAATCATCCATCTAGTTAATAAGAAGAATACCGc                                                                                                             >  2:277793/1‑133 (MQ=255)               tGGTCGGCGACATCGACGACAGCGGGCGTTTGTTCGGCCCGACGGCGTTGGCATCACCGCCCACCAGCCCGTAAACGTTCGGTACGCCCGGTTTGGCGGAGAGATCATCCGTCTAGTTATTAAGCAGAATAcc                                                                                                               >  1:291077/1‑133 (MQ=255)               tGGTCGGCGACATCGACGACAGCGGGCGTTTGTTCGGCCCGACGGCGTTGGCATCACCGCCCACCAGCCCGTAAACGTTCGGTACGCCCGGTTTGGCGGAGAAATCATCCATCTAGTTATTAAGCAGAATAcc                                                                                                               >  1:45140/1‑133 (MQ=255)                     gCGACATCCACGACAGCGGGCG‑‑‑G‑TCGGCCCGACGGCGTTGGCATCACCGCCCACCAGCCCGTAAACGTTCGGTACGCCCGGTTTGGCGGAGAAATCATCCATCTAGTTATTAAGCAGAATACCGCTCTCGccc                                                                                                     <  1:277793/133‑1 (MQ=255)                          aTCGACGACAGCGGGCGTTTGTTCGGCCCGACGGCGTTGGCATCACCGCCCACCAGCCCGTAAACGTTCGGTACGCCCGGTTTGGCGGAGAAATCATCCATCTAGTTATTAAGCAGAATACCGCTCTCGCCcg                                                                                                    <  2:54806/133‑1 (MQ=255)                            cgacgaCAGCGGGCGTTTGTTCGGCCCGACGGCGTTGGCATCACCGCCCACCAGCCCGTAAACGTTCGGTACGCCCGGTTTGGCGGAGAAATCATCCATCTAGTTATTAAGCAGAATACCGCTCTCGCCcgcg                                                                                                  >  1:377806/1‑133 (MQ=255)                                  cAGCGGGCGTTTGTTCGGCCCGACGGCGTTGGCATCACCGCCCACCAGCCCGTAAACGTTCGGTACGCCCGGTTTGGCGGAGAAATCATCCATCTAGTTATTAAGCAGAATACCGCTCTCGCCCGCGACAATg                                                                                            >  2:382087/1‑133 (MQ=255)                                    gCGGGCGTTTGTTCGCCCCGACGGGGTTCGCATCACCGCCCACCAGCCCGTAAACGTTCGGTACGCCCGGTTTGGCGGAGAAATCATCCATCTAGTTATTAAGCAGAATACCGCTCTCGCCCGCGACAATGcc                                                                                          <  2:291077/133‑1 (MQ=255)                                            ttGTTCGGCCAGACGACGTTGGCAGCACAGCCGACCAGCCCGTAAACGTTCGGTACGCCCGGTTTGGCGGAGAAATCATCCATCTAGTTATTAAGCAGAATACCGCTCTCGCCCGCGACAATGCCCGTACCGa                                                                                  <  2:222317/133‑1 (MQ=255)                                                    cccGACGGCGTTGGCATCACCGCCCACCAGCCCGTAAACGTTCGGTACGCCCGGTTTGGCGGAGAAATCATCCATCTAGTTATTAAGCAGAATACCGCTCTCGCCCGCGACAATGCCCGTACCGAAGgtggtg                                                                          >  2:457851/1‑133 (MQ=255)                                                      cGACGGCGTTGGCATCACCGCCCACCAGCCCGTAAACGTTCGGTACGCCCGGTTTGGCGGAGAAATCATCCATCTAGTTATTAAGCAGAATACCGCTCTCGCCCGCGACAATGCCCGTACCGAAGGTGGTGtt                                                                        <  1:630039/133‑1 (MQ=255)                                                      cGACGGCGTTGGCATCACCGCCCACCAGCCCGTAAACGTTCGGTACGCCCGGTTTGGCGGAGAAATCATCCATCTAGTTATTAAGCAGAATACCGCTCTCGCCCGCGACAATGCCCGTACCGAAGGTGGTGtt                                                                        <  1:484618/133‑1 (MQ=255)                                                              ttGGCATCACCGCCCACCAGCCCGTAAACGTTCGGTACGCCCGGTTTGGCGGAGAAATCATCCATCTAGTTATTAAGCAGAATACCGCTCTCGCCCGCGACAATGCCCGTACCGAAGGTGGTGTTCAGCGtat                                                                >  1:106191/1‑133 (MQ=255)                                                                          cccACCAGCCCGTAAACGTTCGGTACGCCCGGTTTGGCGGAGAAATCATCCATCTAGTTATTAAGCAGAATACCGCTCTCGCCCGCGACAATGCCCGTACCGAAGGTGGTGTTCAGCGTATAGGTcaccgcca                                                    <  1:125003/133‑1 (MQ=255)                                                                               caGCCCGTAAACGTTCGGTACGCCCGGTTTGGCGGAGAAATCATCCATCTAGTTATTAAGCAGAATACCGCTCTCGCCCGCGACAATGCCCGTACCGAAGGTGGTGTTCAGCGTATAGGTCACCGCCACcgcg                                               <  1:382087/133‑1 (MQ=255)                                                                                   ccGTAAACGTTCGGTACGCCCGGTTTGGCGGAGAAATCATCCATCTAGTTATTAAGCAGAATACCGCTCTCGCCCGCGACAATGCCCGTACCGAAGGTGGTGTTCAGCGTATAGGTCACCGCCACCGCGTTAc                                           <  2:78424/133‑1 (MQ=255)                                                                                   ccGTAAACGTACGGTTCGCCCGGTTTGGCGGACAAATCATCCATCTAGTTATTAAGCAGAATACCGCTCTCGCCCGCGACAATGCCCGTACCGAAGGTGGTGTTCAGCGTATAGGTCACCGCCACCGCGTTAc                                           <  2:171572/133‑1 (MQ=255)                                                                                        aaCGTTCGGTACGCCCGGTTTGGCGGAGAAATCATCCATCTAGTTATTAAGCAGAATACCGCTCTCGCCCGCGACAATGCCCGTACCGAAGGTGGTGTTCAGCGTATAGGTCACCGCCACCGCGTTACCATCt                                      <  1:638569/133‑1 (MQ=255)                                                                                          cGTTCGGTACGCCCGGTTTGGCGGAGAAATCATCCATCTAGTTATTAAGCAGAATACCGCTCTCGCCCGCGACAATGCCCGTACCGAAGGTGGTGTTCAGCGTATAGGTCACCGCCACCGCGTTACCATCttt                                    <  1:475139/133‑1 (MQ=255)                                                                                               ggTACGCCCGGTTTGGCGGAGAAATCATCCATCTAGTTATTAAGTAGAATACCGCTCTCGCCCGCGACAATGCCCGTACCGAAGGTGGTGTTCAGCGTATAGGTCACCGCCACCGCGTTACCATCTTTATcca                               <  1:624327/133‑1 (MQ=255)                                                                                                  aCGCCCGGTTTGGCGGAGAAATCATCCATCTAGTTATTAAGCAGAATACCGCTCTCGCCCGCGACAATGCCCGTACCGAAGGTGGTGTTCAGCGTATAGGTCACCGCCACCGCGTTACCATCTTTATccacca                            <  2:596365/133‑1 (MQ=255)                                                                                                      ccGGTTTGGCGGAGAAATCATCCATCTAGTTATTAAGCAGAATACCGCTCTCGCCCGCGACAATGCCCGTACCGAAGGTGGTGTTCAGCGTATAGGTCACCGCCACCGCGTTACCATCTTTATCCACCACTGa                        <  2:45140/133‑1 (MQ=255)                                                                                                              gCGGAGAAACAATCCATCTAGTTAATTAACAGTATACCGCTTTCGCCCGCGACAATGCCCGTACCGAAGGTGGTGTTCAGCGTATAGGTCACCGCCACCGCGTTACCATCTTTATCCACCACTGAGTAATggg                <  2:283456/133‑1 (MQ=255)                                                                                                               cGGAGAAATCATCCATCTAGTTATTAAGCAGAATACCGCTCTCGCCCGCGACAATGCCCGTACCGAAGGTGGTGTTCAGCGTATAGGTCACCGCCACCGCGTTACCATCTTTATCCACCACTGAGTAATGGGt               <  2:614215/133‑1 (MQ=255)                                                                                                                 gagaAATCATCCATCTAGTTATTAAGCAGAATACCGCTCTCGCCCGCGACAATGCCCGTACCGAAGGTGGTGTTCAGCGTATAGGTCACCGCCACCGCGTTACCATCTTTATCCACCACTGAGTAATGGGTAg             <  1:133715/133‑1 (MQ=255)                                                                                                                         atccatcTAGTTATTAAGCAGAATACCGCTCTCGCCCGCGACAATGCCCGTACCGAAGGTGGTGTTCAGCGTATAGGTCACCGCCACCGCGTTACCATCTTTATCCACCACTGAGTAATGGGTAGTTTGATTa     <  1:457851/133‑1 (MQ=255)                                                                                                                            catcTAGTTATTAAGCAGAATACCGCTCTCGCCCGCGACAATGCCCGTACCGAAGGTGGTGTTCAGCGTATAGGTCACCGCCACCGCGTTACCATCTTTATCCACCACTGAGTAATGGGTAGTTTGATTActc  <  1:341007/133‑1 (MQ=255)                                                                                                                                 |                                                                                                                                GTCTTTCACCACAATGGTCGGCGACATCGACGACAGCGGGCGTTTGTTCGGCCCGACGGCGTTGGCATCACCGCCCACCAGCCCGTAAACGTTCGGTACGCCCGGTTTGGCGGAGAAATCATCCATCTGGTTATTAAGCAGAATACCGCTCTCGCCCGCGACAATGCCCGTACCGAAGGTGGTGTTCAGCGTATAGGTCACCGCCACCGCGTTACCATCTTTATCCACCACTGAGTAATGGGTAGTTTGATTACTC  >  NC_000913/3585408‑3585663

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑