Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	432,361	T→A	D164V (GAT→GTT)	yajI ←	putative lipoprotein YajI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	432,361	0	T	A	100.0%	75.8 / NA	24	D164V (GAT→GTT)	yajI	putative lipoprotein YajI
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (20/4);  total (20/4)

AATCCAGGTGATGTAAGCAGAGTAACAAAATGACGGGGAAAGATGTTGCGGGACGCACAAAATCGTCCCGAAATACGTTTACTGCATAACAGGTTGAATGTCGTGGATACGTACAAAGCCTAACTGATCCACAGAAATCCCCTTTAACTGTAACGGAATATCAACATCGCTGGGGGCGAGGACGCTGGCAGGGGCATTAATCAATTGATTCTGCACATTGATTTCCTGAAAGTTGTCTATTGTTCCCT  >  NC_000913/432234‑432481                                                                                                                                |                                                                                                                         aaTCCAGGTGATGTAAGCAGAGTAACAAAATGACGGGGAAAGATGTTGCGGGACGCACAAAATCGTCCCGAAATACGTTTACTGCATAACAGGTTGAATGTCGTGGATACGTACAAAGCCTAACTGAACcaca                                                                                                                     >  2:21125/1‑133 (MQ=255) aaTCCAGGTGATGTAAGCAGAGTAACAAAATGACGGGGAAAGATGTTGCGGGACGCACAAAATCGTCCCGAAATACGTTTACTGCATAACAGGTTGAATGTCGTGGATACGTACAAAGCCTAACTGAACcaca                                                                                                                     >  1:586894/1‑133 (MQ=255)               aaGCAGAGTAACAAATTGCCGGCGAAAGATGTTGCGGGACGCACAAAATCGTCCCGAAATACGTTTACTGCATAACAGGTTGAATGTCGTGGATACGTACAAAGCCTAACTGAACCACAGAAATCCCCTTTaa                                                                                                       <  1:205309/133‑1 (MQ=255)                     agTAACAAAATGACGGGGAAAGATGTTGCGGGACGCACAAAATCGTCCCGAAATACGTTTACTGCATAACAGGTTGAATGTCGTGGATACGTACAAAGCCAAACTGAAACACAGAAAACCCCCCTAACTGTaa                                                                                                 >  2:285077/1‑133 (MQ=255)                       tAACAAAATGACGGGGAAAGATGTTGCGGGACGCACAAAATCGTCCCGAAATACGTTTACTGCATAACAGGTTGAATGTCGTGGATACGTACAAAGCCTAACTGGACCACAGAAATCACCTTTAACTGTAACg                                                                                               >  2:244499/1‑133 (MQ=255)                                 aCGGGGAAAGATGTTGCGGGACGCACAAAATCGTCCCGAAATACGTTTACTGCATAACAGGTTGAATGTCGTGGATACGTACAAAGCCTAACTGAACCACAGAAATCCCCTTTAACTGTAACGGAATATCAAc                                                                                     >  2:613101/1‑133 (MQ=255)                                               tGCGGGACGCACAAAATCGTCCCGAAATACGTTTACTGCATAACAGGTTGAATGTCGTGGATACGTACAAAGCCTAACTGAACCACAGAAATCCCCTTTAACTGTAACGGAATATCAACATCGCTGGGGGCGa                                                                       >  2:402105/1‑133 (MQ=255)                                                      cGCACAAAATCGTCCCGAAATACGTTTACTGCATAACAGGTTGAATGTCGTGGATACGTACAAAGCCTAACTGAACCACAGAAATCCCCTTTAACTGTAACGGAATATCAACATCGCTGGGGGCGAGGACGCt                                                                >  1:491327/1‑133 (MQ=255)                                                                cGTCCCGATATACGTTTAATGCATAACAGGCTGAATGTCGTGGATACGTACAAAGCCTAACTGAACCACAGAAATCCCCTTTAACTGTAACGGAATATCAACATCGCTGGGGGCGAGGACGCTGGCAGGGGCa                                                      <  1:285077/133‑1 (MQ=255)                                                                 gTCCCGAAATACGTTTACTGCATAACAGGTTGAATGTCGTGGATACGTACAAAGCCTAACTGAACCACAGAAATCCCCTTTAACTGTAACGGAATATCAACATCGCTGGGGGCGAGGACGCTGGCAGGGGCAt                                                     >  2:416440/1‑133 (MQ=255)                                                                       aaaTACGTTTACTGCATAACAGGTTGAATGTCGTGGATACGTACAAAGCCTAACTGAACCACAGAAATCCCCTTTAACTGTAACGGAATATCAACATCGCTGGGGGCGAGGACGCTGGCAGGGGCATTAATCa                                               >  2:545402/1‑133 (MQ=255)                                                                                      aTAACAGGTTGAATGTCGTGGATACGTACAAAGCCTAACTGAACCACAGAAATCCCCTTTAACTGTAACGGAATATCAACATCGCTGGGGGCGAGGACGCTGGCAGGGGCATTAATCAATTGATTCTGCACAt                                >  2:396760/1‑133 (MQ=255)                                                                                       tAACAGGTTGAATGTCGTGGATACGTACAAAGCCTAACTGAACCACAGAAATCCCCTTTAACTGTAACGGAATATCAACATCGCTGGGGGCGAGGACGCTGGCAGGGGCATTAATCAATTGATTCTGCACAtt                               <  1:21125/133‑1 (MQ=255)                                                                                        aaCAGGTTGAATGTCGTGGATACGTACAAAGCCTAACTGAACCACAGAAATCCCCTTTAACTGTAACGGAATATCAACATCGCTGGGGGCGAGGACGCTGGCGGGGGCATCAATGAAGTGATTCAGCACATTg                              >  1:300288/1‑133 (MQ=255)                                                                                          cAGGTTGAATGTCGCGGATACGTACAAAGCCTAACTGAACCACAGAAATCCCCTTTAACTGTAACGGAATATCAACATCGCTGGGGGCGAGGACGCTGGCAGGGGCATTAATCAATTGATTCTGCACATTGAt                            >  1:332410/1‑133 (MQ=255)                                                                                             gTTGAATGTCGTGGATACGTACAAAGCCTAACTGAACCACAGAAATCCCCTTTAACTGTAACGGAATATCAACATCGCTGGGGGCGAGGACACTGGCAGGCGCAGTAAACAATTGATGCTGCGCATTGATTTc                         >  2:169213/1‑133 (MQ=255)                                                                                               tGAATGTCGTGGATACGTACAAAGCCTAACTGAACCACAGAAATCCCCTTTAACTGTAACGGAATATCAACATCGCTGGGGGCGAGGACGCTGGCAGGGGCATTAATCAATTGATTCTGCACATTGATTTCCt                       <  2:332410/133‑1 (MQ=255)                                                                                               tGAATGTCGTGGATACGTACAAAGCCTAACTGAACCACAGAAATCCCCTTTAACTGTAACGGAATATCAACATCGCTGGGGGCGAGGACGCTGGCAGGGGCATTAATCAATTGATTCTGCACATTGATTTCCt                       >  2:54471/1‑133 (MQ=255)                                                                                                gAATGTCGTGGATACGTACAAAGCCTAACTGAACCACAGAAATCCCCTTTAACTGTAACGGAATATCAACATCGCTGGGGGCGAGGACGCTGGCAGGGGCATTAATCAATTGATTCTGCACATTGATTTCCTg                      >  1:559115/1‑133 (MQ=255)                                                                                                gAATGTCGTGGATACGTACAAAGCCTAACTGAACCACAGAAATCCCCTTTAACTGTAACGGAATATCAACATCGCTGGGGGCGAGGACGCTGGCAGGGGCATAAATCAATTGATTCTACACATTAATTTCCTg                      >  1:291462/1‑133 (MQ=255)                                                                                                       gTGGATACGTACAAAGCCTAACTGAACCACAGAAATCCCCTTTAACTGTAACGGAATATCAACATCGCTGGGGGCGAGGACGCTGGCAGGGACATTAATCAATTGATTCTGCACATTGATTTCCTGAAAGTTg               >  2:231491/1‑133 (MQ=255)                                                                                                               gTACAAAGCCTAACTGAACCACAGAAATCCCCTTTAACTGTAACGGAATATCAACATCGCTGGGGGCGAGGACGCTGGCAGGGGCATTAATCAATTGATTCTGCACATTGATTTGCTGAAAGTTGTCTATTGt       >  1:167010/1‑133 (MQ=255)                                                                                                               gTACAAAGCCTAACTGAACCACAGAAATCCCCTTTAACTGTAACGGAATATCAACATCGCTGGGGGCGAGGACGCTGGCAGGGGCATTAATCAATTGATTCTGCACATTGATTTCCTGAAAGTTGTCTATTGt       >  2:366484/1‑133 (MQ=255)                                                                                                                    aaGCCTAACTGAACCACAGAAATCCCCTTTAACTGTAACGGAATATCAACATCGCTGGGGGCGAGGACGCTGGCAGGGGCATTAATCAATTGATTCTGCACATTGATTTCCTGAAAGTTGTCTATTGTTCCCt  >  2:420217/1‑133 (MQ=255)                                                                                                                                |                                                                                                                         AATCCAGGTGATGTAAGCAGAGTAACAAAATGACGGGGAAAGATGTTGCGGGACGCACAAAATCGTCCCGAAATACGTTTACTGCATAACAGGTTGAATGTCGTGGATACGTACAAAGCCTAACTGATCCACAGAAATCCCCTTTAACTGTAACGGAATATCAACATCGCTGGGGGCGAGGACGCTGGCAGGGGCATTAATCAATTGATTCTGCACATTGATTTCCTGAAAGTTGTCTATTGTTCCCT  >  NC_000913/432234‑432481

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑