Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,099,002 T→C G119G (GGT→GGC)  rhaR → DNA‑binding transcriptional activator RhaR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,099,0020TC93.1% 93.4 / NA 29G119G (GGT→GGC) rhaRDNA‑binding transcriptional activator RhaR
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (17/10);  minor base G (1/1);  total (18/11)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGCGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCGGCAGGTTATCGGTCAGCTTGAGCATGAAAGTAGTCAGCATGTGCCGTTTGCTAACGAAATGGCTGAGTTGCTGTTCGGGCAGTTGGTGATGTTGCTGAATCGCC  >  NC_000913/4098874‑4099129
                                                                                                                                |                                                                                                                               
gCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGCGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGgcagca                                                                                                                             <  2:323710/133‑1 (MQ=255)
  ttCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGCGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGgcagca                                                                                                                             >  2:554262/1‑131 (MQ=255)
  ttCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGCGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGgcagca                                                                                                                             <  1:554262/131‑1 (MQ=255)
    ccGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGCGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGCAGCATggg                                                                                                                         >  1:150085/1‑133 (MQ=255)
       ttAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGCGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCGACCACACAGGCTCTAAGGCAGGATGGGGAt                                                                                                                      >  1:173093/1‑133 (MQ=255)
              tCTGGTTTTGCAGAATATTATTTATTGCCCGGAGCGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACATTGGCGCTGAGGGAGGATGGGGGTGGCGCAg                                                                                                               >  1:176641/1‑133 (MQ=255)
                 ggTTTTGCAGTATATAATTTATTTCCTGCAGCATCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGCAGCATGGGGATGGCGCAGgcg                                                                                                            <  2:295930/133‑1 (MQ=255)
                      tGCAGAATATTATTTATTGCCCGGAGCGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGCAGCATGGGGACGGCGCAAGCGCGGCa                                                                                                       >  1:282607/1‑133 (MQ=255)
                                     attGCCCGGAGCGTCTGAAGCTGAATCTTGACTGGCGGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGCAGCATGGGGATGGCGCAGGCGCGGCAGGTTATCGGTCAGCt                                                                                        >  1:456379/1‑133 (MQ=255)
                                       tGCCCGGAGCGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGCAGCATGGGGATGGCGCAGGCGCGGGAGGTTATCGGTCAGCTTg                                                                                      >  1:165495/1‑133 (MQ=255)
                                         cccGGAGCGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGCAGCATGGGGATGGCGCAGGCGCGGCAGGTTATCGGTCAGCTTGAg                                                                                    >  1:601971/1‑133 (MQ=255)
                                           ccgAGCGTCGTAAGCAGAATCTTATCAGTCAGCGGGCGATTACGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGCAGCATGGGGATGGCGCAGGCGCGGCAGGTTATCGGTCAGCTTGAGCa                                                                                  <  2:302756/131‑1 (MQ=255)
                                           cGGAGCGTCTCAAGCCGAATCTTGCCTGCCAGCGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGCAGCATGGGGATGGCGCAGGCGCGGCAGGTTATCGGTCAGCTTGAGCa                                                                                  <  2:165495/133‑1 (MQ=255)
                                             gAGCGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGCAGCATGGGGATGGCGCAGACGCGGCAGGTTCTCAGTCagcatgagcatg                                                                                >  1:208414/1‑133 (MQ=255)
                                                      aaGCTGAATCTTGACTGGCAGGGGGCGATTCTGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGCAGCATGGGGATGGCGCAGGCGCGGCAGGTTATCGGTCAGCTTGAGCATGAAAGTAGTc                                                                       <  2:520447/133‑1 (MQ=255)
                                                            aaTCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGCAGCATGGGGATGGCGCAGGCGCCGCAGGTTAACGGACAGGTTGAGCATGAAAGCAGTCAGCAtg                                                                 >  2:217412/1‑133 (MQ=255)
                                                                 tGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGCAGCATGGGGATGGCGCAGGCGCGGCAGGTTATCGGTCAGCTTGAGCATGAAAGTAGTCAGCATGTGCCg                                                            >  2:25355/1‑133 (MQ=255)
                                                                   aCTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGCAGCATGGGGATGGCGCAGGCGCGGCAGGGTATCGGTCAGCATGAGCATGAAAGTAGTCAGCATGTGCCGtt                                                          >  1:255595/1‑133 (MQ=255)
                                                                      ggCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGCAGCATGGGGATGGCGCAGGCGCGGCAGGTTATCGGTCAGCTTGAGCATGAAAGTAGTCAGCATGTGCCGTTTGc                                                       >  1:635758/1‑133 (MQ=255)
                                                                                  ttCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGCAGCATGGGGAGGGCGCAGGCGCGGCAGGTTATCGGTCAGCTTGAGCATGAAAGTAGTCAGCATGTGCCGTTTGCTAACGAAATGGc                                           >  1:611176/1‑133 (MQ=255)
                                                                                       ggATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGCAGCATGGGGATGGCGCAGGCGCGGCAGGTTATCGGTCAGCTTGAGCATGAAAGTAGTCAGCATGTGCCGTTTGCTAACGAAATGGCTGAGt                                      >  1:244258/1‑133 (MQ=255)
                                                                                                gCCAGCGCAGGGCAACCACACTGGCGCTTAGGCAGCATGGGGATGGCGCAGGCGCGGCAGGTTATCGGTCAGCTTGAGCATGAAAGTAGTCAGCATGTGCCGTTTGCTAACGAAATGGCTGAGTTGCTGTTCg                             <  2:341255/133‑1 (MQ=255)
                                                                                                  cAGCGCAGGGCAACCACACTGGCGCTTAGGCAGCATGGGGATGGCGCAGGCGCGGCAGGTTATCGGTCAGCTTGAGCATGAAAGTAGTCAGCATGTGCCGTTTGCTAACGAAATGGCTGAGTTGCTGTTCggg                           <  2:244258/133‑1 (MQ=255)
                                                                                                   aGCGCAGGGCAACCACACTGGCGCGTAGTGAGCATGGGGATTGCGCAGGCGCGGCAGGTTATCGGTCAGCTTGAGCATGAAAGTAGTCAGCATGTGCCGTTTGCTAACGAAATGGCTGAGTTGCTGTTCGGGc                          <  2:255595/133‑1 (MQ=255)
                                                                                                     cgcAGGGCAACCACACTGGCGCTTAGGCAGCATGGGGATGGCGCAGGCGCGGCAGGTTATCGGTCAGCTTGAGCATGAAAGTAGTCAGCATGTGCCGTTTGCTAACGAAATGGCTGAGTTGCTGTTCGGGCAg                        >  2:459744/1‑133 (MQ=255)
                                                                                                         gggCAACCACACTGGCGCTTAGGCAGCATGGGGATGGCGCAGGCGCGGCAGGTTATCGGTCAGCTTGAGCATGAAAGTAGTCAGCATGTGCCGTTTGCTAACGAAATGGCTGAGTTGCTGTTCGGGCAGTTgg                    >  2:435210/1‑133 (MQ=255)
                                                                                                               ccACACTGGCGCTTAGGCAGCATGGGGATGGCGCAGGCGCGGCAGGTTATCGGTCAGCTTGAGCATGAAAGTAGTCAGCATGTGCCGTTTGCTAACGAAATGGCTGAGTTGCTGTTCGGGCAGTTGGTGATGt              >  2:470994/1‑133 (MQ=255)
                                                                                                                        cgattAGGCAGCATGGGGATGGCGCAGGCGCGGCAGGTTATCGGTCAGCTTGAGCATGAAAGTAGTCAGCATGTGCCGTTTGCTAACGAAATGGCTGAGTTGCTGTTCGGGCAGTTGGTGATGTTGCTGAATc     >  2:408193/4‑133 (MQ=255)
                                                                                                                         gcTTAGGCAGCATGGGGATGGCGCAGGCGCGGCAGGTTATCGGTCAGCTTGAGCATGAAAGTAGTCAGCATGTGCCGTTTGCTAACGAAATGGCTGAGTTGCTGTTCGGGCAGTTGGTGATGTTGCTGAATCg    <  1:470994/133‑1 (MQ=255)
                                                                                                                           ttAGGCAGCATGGGGCTGGCGCAGCCGCTGCATGTTATCGGCCAGCTTGAGCATGAAAGTAGTCAGCATGTGCCGTTTGCTAACGAAATGGCTGAGTTGCTGTTCGGGCAGTTGGTGATGTTGCTGAATCGcc  <  2:176641/133‑1 (MQ=255)
                                                                                                                           ttAGGCAGCATGGGGATGGCGCAGGCGCGGCAGGTTATCGGTCAGCTTGAGCATGAAAGTAGTCAGCATGTGCCGTTTGCTAACGAAATGGCTGAGTTGCTGTTCGGGCAGTTGGTGATGTTGCTGAATCGcc  <  1:459744/133‑1 (MQ=255)
                                                                                                                           ttAGGCAGCAAGGGGATGGCGCAGGCGCGGCATGTTATCGGCCAGCTTGAGCATGAAAGTAGTCAGCATGTGCCGTTTGCTAACGAAATGGCTGAGTTGCTGTTCGGGCAGTTGGTGATGTTGCTGAATCGcc  <  2:208414/133‑1 (MQ=255)
                                                                                                                                |                                                                                                                               
GCTTCCGTTAACGATCTGGTTTTGCAGAATATTATTTATTGCCCGGAGCGTCTGAAGCTGAATCTTGACTGGCAGGGGGCGATTCCGGGATTTAACGCCAGCGCAGGGCAACCACACTGGCGCTTAGGTAGCATGGGGATGGCGCAGGCGCGGCAGGTTATCGGTCAGCTTGAGCATGAAAGTAGTCAGCATGTGCCGTTTGCTAACGAAATGGCTGAGTTGCTGTTCGGGCAGTTGGTGATGTTGCTGAATCGCC  >  NC_000913/4098874‑4099129

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑