Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,152,837	A→G	Y88H (TAC→CAC)	ppc ←	phosphoenolpyruvate carboxylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,152,837	0	A	G	100.0%	75.8 / NA	22	Y88H (TAC→CAC)	ppc	phosphoenolpyruvate carboxylase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (13/9);  total (13/9)

CAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGGTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCGGGTTGCTGGCAGCTTCGCCTTTCGGCGAAATGCTGTGGTATTGCTCGGCGGTGTTGGCCAGGTTCAGGAACTGACTAAACGCACGCGCAACGGGCAGCAGCTCGTCGTTCGACAAATTTTGTAAGGTGGTGAGCAACTCCTGGCGGTTAGCATCATTGCCA  >  NC_000913/4152712‑4152958                                                                                                                              |                                                                                                                          cagcgaTTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGGTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCGGGTTGCTGGCAGCTTCGCCTTTCGGCGAAATGCTGTGGTGTTGCTcg                                                                                                                    >  2:40044/1‑133 (MQ=255)  agcgaTTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGGTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCGGGTTGCTGGCAGCTTCGCCTTTCGGCGAAATGCTGTGGTGTTGCTcgg                                                                                                                   <  2:497493/133‑1 (MQ=255)                  ttttGATGCTGTCTTCGCTCAGTTCCGGCTAGTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCGGGTTGCTGGCAGCTTCGCCTTTCGGCGAAATGCTGTGGTGTTGCTCGGCGGTGTTGGCCAGGtt                                                                                                   <  1:257027/133‑1 (MQ=255)                        tGGTGTCTTCGCTCAGTTCCGGCTGGTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCGGGTTGCTGGCAGCTTCGCCTTTCGGCGAAATGCTGTGGTGTTGCTCGGCGGTGTTGGCCAGGTTCAGGaa                                                                                             <  2:133270/133‑1 (MQ=255)                         ggTGTCTTCGCTCAGTTCCGGCTGGTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCGGGTTGCTGGCAGCTTCGCCTTTCGGCGAAATGCTGTGGTGTTGCTCGGCGGTGTTGGCCAGGTTCAGGAAc                                                                                            >  2:526516/1‑133 (MQ=255)                                    tCAGTTCCGGCTGGTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCGGGTTGCTGGCAGCTTCGCCTTTCGGCGAAATGCTGTGGTGTTGCTCGGCGGTGTTGGCCAGGTTCAGGAACTGACTAAacgc                                                                                 >  1:128122/1‑133 (MQ=255)                                       gTTCCGGCTGGTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCGGGTTGCTGGCAGCTTCGCCTTTCGGCGAAATGCTGTGGTGTTGCTCGGCGGTGTTGGCCAGGTTCAGGAACTGACTAAacgcacg                                                                              >  2:155960/1‑133 (MQ=255)                                             gCTGGTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCGGGTTGCTGGCAGCTTCGCCTTTCGGCGAAATGCTGTGGTGTTGCTCGGCGGTGTTGGCCAGGTTCAGGAACTGACTAAACGCACGCGCAAc                                                                        >  1:428962/1‑133 (MQ=255)                                                                    gTGCGGGCGATCACTTCCGGGTTGCTGGCAGCTTCGCCTTTCGGCGAAATGCTGTGGTGTTGCTCGGCGGTGTTGGCCAGGTTCAGGGACTGACTAAACGCAGGCGCAACGGGCGGCAACTCGTGGTTCGACa                                                 >  1:172027/1‑133 (MQ=255)                                                                       cGGGCGATCACTTCCGGGTTGCTGGCAGCTTCGCCTTTCGGCGAAATGCTGTGGTGTTGCTCGGCGGTGTTGGCCAGGTTCAGGAACTGACTAAACGCACGCGCAACGGGCAGCAGCTCGTCGTTCGACAAAt                                              >  1:382054/1‑133 (MQ=255)                                                                        gggCGATCACTTCCGGGTTGCTGGCAGCTTCGCCTTTCGGCGAAATGCTGTGGTGTTGCTCGGCGGTGTTGGCCAGGTTCAGGAACTGACTAAACGCACGCGCAACGGGCAGCAGCTCGTCGTTCGACAAAtt                                             <  1:118645/133‑1 (MQ=255)                                                                          gCGATCACTTCCGGGGTGTTGGCAGCTTCGTCTTTCGGCGAAATGCTGTGGTGTTGCTCGGCGGTGTTGGCCAGGTTCAGGAACTGACTAAACGCACGCGCAACGGGCAGCAGCTCGTCGTTCGACAAAtttt                                           <  1:185498/133‑1 (MQ=255)                                                                             aTCACTTCCGGGTTGCTGGCAGCTTCGCCTTTCGGCGAAATGCTGTGGTGTTGCTCGGCGGTGTTGGCCAGGTTCAGGAACTGACTAAACGCACGCGCAACGGGCAGCAGCTCGTCGTTCGACAAATTTTGTa                                        >  2:629285/1‑133 (MQ=255)                                                                             aTCACTTCCGGGTTGCTGGCAGCTTCGCCTTTCGGCGAAATGCTGTGGTGTTGCTCGGCGGTGTTGGCCAGGTTCAGGAACTGACTAAACGCACGCGCAACGGGCAGCAGCTCGTCGTTCGACAAATTTTGTa                                        >  2:424296/1‑133 (MQ=255)                                                                             aTCACTTCCGGGTTGCTGGCAGCTTCGCCTTTCGGCGAAATGCTGTGGTGTTGCTCGGCGGTGTTGGCCAGGTTCAGGAACTGACTAAACGCACGCGCAACGGGCAGCAGCTCGTCGTTCGACAAATTTTGTa                                        >  2:328014/1‑133 (MQ=255)                                                                             aTCACTTCCGGGTTGCTGGCAGCTTCGCCTTTCGGCGAAATGCTGTGGTGTTGCTCGGCGGTGTTGGCCAGGTTCAGGAACTGACTAAACGCACGCGCAACGGGCAGCAGCTCGTCGTTCGACAAATTTTGTa                                        <  1:346232/133‑1 (MQ=255)                                                                             aTCACTTCCGCGTTGCTGGCAGCTCCGCCTTTTGGCGAAATCCTGTGGTGTTGCTCGGCGGTGTTGGCCAGGTTCAGGAACTGACTAAACGCACGCGCAACGGGCAGCAGCTCGTCGTTCGACAAATTTTGTa                                        <  2:164120/133‑1 (MQ=255)                                                                               cACTTCCGGGTTGCTGGCAGCTTCGCCTTTCGGCGAAATGCTGTGGTGTTGCTCGGCGGTGTTGGCCAGGTTCAGGAACTGACTAAACGCACGCGCAACGGGCAGCAGCTCGTCGTTCGACAAATTTTGTAAg                                      >  1:547155/1‑133 (MQ=255)                                                                                    ccGGGTTGCTGGCAGCTTCGCCTTTCGGCGAAATGCTGTGGTGTTGCTCGGCGGTGTTGGCCAGGTTCAGGAACTGACTAAACGCACGCGCAACGGGCAGCAGCTCGTCGTTCGACAAATTTTGTAAggtggt                                 >  1:504199/1‑133 (MQ=255)                                                                                                     tCGCCTTTCGGCGAAATGCTGTGGTGTTGCTCGGCGGTGTTGGCCAGGTTCAGGAACTGACTAAACGCACGCGCAACGGGCAGCAGCTCGTCGTTCGACAAATTTTGTAAGGTGGTGAGCAACTCCTGGCGGt                <  2:504199/133‑1 (MQ=255)                                                                                                                   aaTGCTGTGGTGTTGCTCGGCGGTGTTGGCCAGGTTCAGGAACTGACTAAACGCACGCGCAACGGGCAGCAGCTCGTCGTTCGACAAATTTTGTAAGGTGGTGAGCAACTCCTGGCGGTTAGCATCATTGccc  >  1:489875/1‑132 (MQ=255)                                                                                                                   aaTGCTGTGGTGTTGCTCGGCGGTGTTGGCCAGGTTCAGGAACTGACTAAACGCACGCGCAACGGGCAGCAGCTCGTCGTTCGACAAATTTTGTAAGGTGGTGAGCAACTCCTGGCGGTTAGCATCATTGCCa  <  1:361552/133‑1 (MQ=255)                                                                                                                              |                                                                                                                          CAGCGATTCCACTGCTTTTTTGATGGTGTCTTCGCTCAGTTCCGGCTGGTTTTTCAGTTTACGCAGGGTGCGGGCGATCACTTCCGGGTTGCTGGCAGCTTCGCCTTTCGGCGAAATGCTGTGGTATTGCTCGGCGGTGTTGGCCAGGTTCAGGAACTGACTAAACGCACGCGCAACGGGCAGCAGCTCGTCGTTCGACAAATTTTGTAAGGTGGTGAGCAACTCCTGGCGGTTAGCATCATTGCCA  >  NC_000913/4152712‑4152958

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑