| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,226,892 | T→C | V1022A (GTG→GCG) | metH → | cobalamin‑dependent methionine synthase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,226,892 | 0 | T | C | 96.0% | 84.6 / NA | 25 | V1022A (GTG→GCG) | metH | cobalamin‑dependent methionine synthase |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (11/13); minor base A (1/0); total (12/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.80e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ACGACATGCTGGATAAATTAAGCGCCGAGAAAACGCTGAATCCGCGTGGCGTGGTGGGCCTGTTCCCGGCAAACCGTGTGGGCGATGACATTGAAATCTACCGTGACGAAACGCGTACCCATGTGATCAACGTCAGCCACCATCTGCGTCAACAGACCGAAAAAACAGGCTTCGCTAACTACTGTCTCGCTGACTTCGTTGCGCCGAAGCTTTCTGGTAAAGCAGATTACATCGGCGCATTTGCCGTG > NC_000913/4226769‑4227016 | aCGACATGCTGGATAAATTAAGCGCCGAGAAAACGCTGAATCCGCGTGGCGTGGTGGGCCTGTTCCCGGCAAACCGTGTGGGCGATGACATTGAAATCTACCGTGACGAAACGCGTACCCATGCGATCAACGt < 1:363244/133‑1 (MQ=255) tGGATAAATTAAGCGTCGCGAATACGCTGACTCCGCGTGGCGTGGTGGGCCTGTTCCCGGCAAACCGTGTGGGCGATGACATTGAAATCTACCGTGACGAAACGCGTACCCATGCGATCAACGTCAGccacca < 1:183742/133‑1 (MQ=255) tGGATAAACTAAGCGACGTGAACACGCTGACTCCGCGTGGCGTGGTGGGCCTGTTCCCGGCAAACCGTGTGGGCGATGACATTGAAATCTACCGTGACGAAACGCGTACCCATGCGATCAACGTCAGccacca < 1:298335/133‑1 (MQ=255) gcgcCGAGAAAACGCTGAATCCGCGTGGCGTGGTGGGCCTGTTCCCGGCAAACCGTGTGGGCGATGACATTGAAATCTACCGTGACGAAACGCGTACCCATGCGATCAACGTCAGCCACCATCTGCGTCAACa > 2:79416/1‑133 (MQ=255) gcgcCGAGAAAACGCTGAATCCGCGTGGCGTGGTGGGCCTGTTCCCGGCAAACCGTGTGGGCGATGACATTGAAATCTACCGTGACGAAACGCGTACCCATGCGATCAACGTCAGCCACCATCTGCGTCAACa > 2:474743/1‑133 (MQ=255) gcgcCGAGAAAACGCTGAATCCGCGTGGCGTGGTGGGCCTGTTCCCGGCAAACCGTGTGGGCGATGACATTGAAATCTACCGTGACGAAACGCGTACCCATGAGATCAACGTCAACCACCATCTACGTCAACa > 1:290028/1‑133 (MQ=255) cGAGAAAACGCTGAATCCGCGTGGCGTGGTGGGCCTGTTCCCGGCAAACCGTGTGGGCGATGACATTGAAATCTACCGTGACGAAACGCGTACCCATGCGATCAACGTCAGCCACCATCTGCGTCAACAGAcc < 2:471017/133‑1 (MQ=255) gagaAAACGCTGAATCCGCGTGGCGTGGTGGGCCTGTTCCCGGCAAACCGTGTGGGCGATGACATTGAAATCTACCGTGACGAAACGCGTACCCATGCGATCAACGTCAGCCACCATCTGCGTCAACAGACCg > 2:568766/1‑133 (MQ=255) agaAAACGCTGAATCCGCGTGGCGTGGTGGGCCTGTTCCCGGCAAACCGTGTGGGCGATGACATTGAAATCTACCGTGACGAAACGCGTACCCATGCGATCAACGTCAGCCACCATCTGCGTCAACAGACCGa > 1:642271/1‑133 (MQ=255) aaTCCGCGTGGCGTGGTGGGCCTGTTCCCGGCAAACCGTGTGGGCGATGACATTGAAATCTACCGTGACGAAACGCGTACCCATGCGATCAACGTCAGCCACCATCTGCGTCAACAGACCGAAAAAACAGGCt < 1:471565/133‑1 (MQ=255) aaTCCGCGTGGCGTGGTGGGCCTGTTCCCGGCAAACCGTGTGGGCGATGACATTGAAATCTACCGTGACGAAACGCGTACCCATGCGATCAACGTCAGCCACCATCTGCGTCAACAGACCGAAAAAACAGGCt < 1:474743/133‑1 (MQ=255) aaTCCGCGTGGCGTGGTGGGCCTGTTCCCGGCAAACCGTGTGGGCGATGACATTGAAATCTACCGTGACGAAACGCGTACCCATGCGATCAACGTCAGCCACCATCTGCGTCAACAGACCGAAAAAACAGGCt < 1:79416/133‑1 (MQ=255) aaTCCGCGTGGCGTGGTGGGCCTGTTCCCGGCAAACCGTGTGGGCGATGACATTGAAATCTACCGTGACGAAACGCGTACCCATGCGATCAACGTCAGCCACCATCTGCGTCAACAGACCGAAAAAACAGGCt < 2:433865/133‑1 (MQ=255) tacgcgTGGCCTGGTGGGCCTGTTTCGGCCAACCCGTGTGGGCGATGACATTGAAATCTACCGTGACGAAACGCGTACCCATGCGATCAACGTCAGCCACCATCTGCGTCAACAGACCGAAAAAACAGGCTTc < 2:290028/131‑1 (MQ=255) gcgtggcgtgGTGGGCCTGTTCCCGGCAAACCGTGTGGGCGATGACATTGAAATCTACCGTGACGAAACGCGTACCCATGCGATCAACGTCAGCCACCATCTGCGTCAACAGACCGAAAAAACAGGCTTCGCt < 1:388909/133‑1 (MQ=255) cgtggcgtggTGGGCCTGTTCCCGGCAAACCGTGTGGGCGATGACATTGAAATCTACCGTGACGAAACGCGTACCCATGCGATCAACGTCACCCACCATCTGCGGCAAGAGACCGAAAAAACAGGCTTCGCTa > 2:212193/1‑133 (MQ=255) gcgtggTGGGCCTGTTCCCGGCAAACCGTGTGGGCGATGACATTGAAATCTACCGTGACGAAACGCGTACCCATGCGATCAACGTCAGCCACCATCTGCGTCAACAGACCGAAAAAACAGGCTTCGCTAacta > 1:526461/1‑133 (MQ=255) gcgtggTGGGCCTGTTCCCGGCAAACCGTGTGGGCGATGACATTGAAATCTACCGTGACGAAACGCGTACCCATGCGATCAACGTCAGCCACCATCTGCGTCAACAGACCGAAAAAACAGGCTTCGCTAacta > 1:579701/1‑133 (MQ=255) gcgtggTGGGCCTGTTCCCGGCAAACCGTGTGGGCGATGACATTGAAATCTACCGTGACGAAACGCGTACCCATGCGATCAACGTCAGCCACCATCTGCGTCAACAGACCGAAAAAACAGGCTTCGCTAacta < 2:526461/133‑1 (MQ=255) cGGCAAACGGTGTGGTCGCTGAAATTGAAATCTACCGTGACGAAACGCGTACCCATGCGATCAACGTCAGCCACCATCTGCGTCAACAGACCGAAAAAACAGGCTTCGCTAACTACTGTCTCGCTGACTTCGt < 1:212193/133‑1 (MQ=255) cGGCAAACCGTGTGGGCGATGACATTGAAATCTACCGTGACGAAACGCGTACCCATGCGATCAACGTCAGCCACCATCTGCGTCAACAGACCGAAAAAACAGGCTTCGCTAACTACTGTCTCGCTGACTTCGt < 1:477818/133‑1 (MQ=255) aCCGTGTGGGCGATGACATTGAAATCTACCGTGACGAAACGCGTACCCATGCGATCAACGTCAGCCACCATCTGCGTCAACAGACCGAAAAAACAGGCTTCGCTAACTACTGTCTCGCTGACTTCGTTGCGcc > 2:389208/1‑133 (MQ=255) aCCGTGTGGGCGATGACATTGAAATCTACCGTGACGAAACGCGTACCCATGCGATCAACGTCAGCCACCATCTGCGTCAACAGACCGAAAAAACAGGCTTCGCTAACTACTGTCTCGCTGACTTCGTTGCGcc > 2:377389/1‑133 (MQ=255) gTACCCATGCGATCAACGTCAGCCACCATCTGCGTCAACAGACCGAAAAAACAGGCTTCGCTAACTACTGTCTCGCTGACTTCGTTGCGCCGAAGCTTTCTGATAAAGCAAATTACAACGGCGCGTTTGCCGt > 1:255494/1‑133 (MQ=255) tACCCATGCGATCAACGTCAGCCACCATCTGCGTCAACAGACCGAAAAAACAGGCTTCGCTAACTACTGTCTCGCTGACTTCGTTGCGCCGAAGCTTTCTGGTAAAGCAGATTACATCGGCGCATTTGCCGTg > 2:99434/1‑133 (MQ=255) | ACGACATGCTGGATAAATTAAGCGCCGAGAAAACGCTGAATCCGCGTGGCGTGGTGGGCCTGTTCCCGGCAAACCGTGTGGGCGATGACATTGAAATCTACCGTGACGAAACGCGTACCCATGTGATCAACGTCAGCCACCATCTGCGTCAACAGACCGAAAAAACAGGCTTCGCTAACTACTGTCTCGCTGACTTCGTTGCGCCGAAGCTTTCTGGTAAAGCAGATTACATCGGCGCATTTGCCGTG > NC_000913/4226769‑4227016 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |