Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,439,090	A→G	V58A (GTG→GCG)	ytfJ ←	protein YtfJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,439,090	0	A	G	100.0%	152.5 / NA	42	V58A (GTG→GCG)	ytfJ	protein YtfJ
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (24/18);  total (24/18)

GGTGGTGGTCTGGTAACGATCGTGCGGTAACTTCGCTGATTTAATCGCTTCAATCAGCGTCGCGTTTTTCTCTTTTGCAGAGGTGCGACCAGCAATATGTTGCAGTACTCGCACTTTTCCCACTAACTGCGCGCTGTTCCAGGTTTTGTAGCTAAACTGATCTTTATCAAGCACCAACTCGCCCCGATCGGTAATGCCAATCGGCGGCACTCGCTGACCGGTTTCGAACTGATGTGCGGAAGCCATCAT  >  NC_000913/4438969‑4439217                                                                                                                          |                                                                                                                                ggtggtggtCTGGTAACGATCGTGCGGTAACTTCGCTGATTTAATCGCTTCAATCAGCGTCGCGTTTTTCTCTTTTGCAGAGGTGCGACCAGCAATATGTTGCAGTACTCGCACTTTTCCCGCTAACTgcgcg                                                                                                                      <  2:346131/133‑1 (MQ=255) ggtggtggtCTGGTAACGATCGTGCGGTAACTTCGCTGATTTAATCGCTTCAATCAGCGTCGCGTTTTTCTCTTTTGCAGAGGTGCGACCAGCAATATGTTGCAGTACTCGCACTTTTCCCGCTAACTgcgcg                                                                                                                      <  2:636609/133‑1 (MQ=255) ggtggtggtAGGGTAACGATCGTGCGATAACTTCGCTGATTTAATCGCTTCAATCAGCGTCGCGTTTTTCTCTTTTGCAGAGGTGCGACCAGCAATATGTTGCAGTACTCGCACTTTTCCCGCTAACTgcgcg                                                                                                                      <  2:273349/133‑1 (MQ=255)      tggtCTGGTAACGATCGTGCGGTAACTTCGCTGATTTAATCGCTTCAATCAGCGTCGCGTTTTTCTCTTTTGCAGAGGTGCGACCAGCAATATGTTGCAGTACTCGCACTTTTCCCGCTAACTGCGCGCTGtt                                                                                                                 >  1:370698/1‑133 (MQ=255)        gtCTGGTAACCATCGTGCGGTAACCTCGCTGATTTAATCGCCTCAATCAGCGTCGCGTTTTTCTCTTTTGCAGAGGTGCGACCAGCAATATGTTGCAGTACTCGCACTTTTCCCGCTAACTGCGCGCTGTTcc                                                                                                               <  2:179099/133‑1 (MQ=255)                   aTCGTGCGGTAACTTCGCTGATTTAATCGCTTCAATCAGCGTCGCGTTTTTCTCTTTTGCAGAGGTGCGACCAGCAATATGTTGCCGTCCTCGCACTTTTCCCGCTAACTGCGCGCTGTTCCAGGTTTTGTAg                                                                                                    <  1:441014/133‑1 (MQ=255)                   aTCGTGCGGTAACTTCGCTGATTTAATCGCTTCAATCAGCGTCGCGTTTTTCTCTTTTGCAGAGGTGCGACCAGCAATATGTTGCAGTACTCGCACTTTTCCCGCTAACTGCGCGCTGTTCCAGGTTTTGTAg                                                                                                    <  2:419771/133‑1 (MQ=255)                   aTCGTGCGGTAACTTCGCTGATTTAATCGCTTCAATCAGCGTCGCGTTTTTCTCTTTTGCAGAGGTGCGACCAGCAATATGTTGCAGTACTCGCACTTTTCCCGCTAACTGCGCGCTGTTCCAGGTTTTGTAg                                                                                                    <  2:47906/133‑1 (MQ=255)                   aTCGTGCGGTAACTTCGCTGATTTAATCGCTTCAATCAGCGTCGCGTTTTTCTCTTTTGCAGAGGTGCGACCAGCAATATGTTGCAGTACTCGCACTTTTCCCGCTAACTGCGCGCTGTTCCAGGTTTTGTAg                                                                                                    >  2:362179/1‑133 (MQ=255)                   aTCGTGCGGTAACTTCGCTGATTTAATCGCTTCAATCAGCGTCGCGTTTTTCTCTTTTGCAGAGGTGCGACCAGCAATATGTTGCAGTACTCGCACTTTTCCCGCTAACTGCGCGCTGTTCCAGGTTTTGTAg                                                                                                    >  2:53506/1‑133 (MQ=255)                    tCGTGCGGTAACTTCGCTGATTTAATCGCTTCAATCAGCGTCGCGTTTTTCTCTTTTGCAGAGGTGCGACCAGCAATATGTTGCAGTACTCGCACTTTTCCCGCTAACTGCGCGCTGTTCCAGGTTTTGTAGc                                                                                                   <  2:82159/133‑1 (MQ=255)                       tGCGGTAACTTCGCTGATTTAATCGCTTCAATCAGCGTCGCGTTTTTCTCTTTTGCAGAGGTGCGACCAGCAATATGTTGCAGTACTCGCACTTTTCCCGCTAACTGCGCGCTGTTCCAGGTTTTGTAGCTaa                                                                                                >  2:523130/1‑133 (MQ=255)                       tGCGGTAACTTCGCTGATTTAATCGCTTCAATCAGCGTCGCGTTTTTCTCTTTTGCAGAGGTGCGACCAGCAATATGTTGCAGTACTCGCACTTTTCCCGCTAACTGCGCGCTGTTCCAGGTTTTGTAGCTaa                                                                                                >  1:93994/1‑133 (MQ=255)                       tGCGGTAACTTCGCTGATTTAATCGCTTCAATCAGCGTCGCGTTTTTCTCTTTTGCAGAGGTGCGACCAGCAATATGTTGCAGTACTCGCACTTTTCCCGCTAACTGCGCGCTGTTCCAGGTTTTGTAGCTaa                                                                                                >  1:576984/1‑133 (MQ=255)                              aCTTCGCTGATTTAATCGCTTCAATCAGCGTCGCGTTTTTCTCTTTTGCAGAGGTGCGACCAGCAATATGTTGCAGTACTCGCACTTTTCCCGCTAACTGCGCGCTGTTCCAGGTTTTGTAGCTAAACTGAt                                                                                          <  1:128327/132‑1 (MQ=255)                              aCTTCGCTGATTTAATCGCTTCAATCAGCGTCGCGTTTTTCTCTTTTGCAGAGGTGCGACCAGCAATATGTTGCAGTACTCGCACTTTTCCCGCTAACTGCGCGCTGTTCCAGGTTTTGTAGCTAAACTGAt                                                                                          >  2:128327/1‑132 (MQ=255)                              aCTTCGCTGATTTAATCGCTTCAATCAGCGTCGCGTTTTTCTCTTTTGCAGAGGTGCGACCAGCAATATGTTGCAGTACTCGCACTTTTCCCGCTAACTGCGAGCTGTTCGAGGATTAGTAGCTTAACTGATc                                                                                         >  1:195753/1‑133 (MQ=255)                                    cTGATTTAATCGCTTCAATCAGCGTCGCGTTTTTCTCTTTTGCAGAGGTGCGACCAGCAATATGTTGCAGTACTCGCACTTTTCCCGCTAACTGCGCGCTGTTCCAGGTTTTGTAGCTAAACTGATCTTTATc                                                                                   <  2:434709/133‑1 (MQ=255)                                                 ttCAATCAGCGTCGCGTTTTTCTCTTTTGCAGAGGTGCGACCAGCAATATGTTGCAGTACTCGCACTTTTCCCGCTAACTGCGCGCTGTTCCAGGTTTTGTAGCTAAACTGATCTTTATCAAGCACCAACTCg                                                                      >  1:592170/1‑133 (MQ=255)                                                 ttCAATCAGCGTCGCGTTTTTCTCTTTTGCAGAGGTGCGACCAGCAATATGTTGCAGTACTCGCACTTTTCCCGCTAACTGCGCGCTGTTCCAGGTTTTGTAGCTAAACTGATCCTTTTCAAGCACCAACTCg                                                                      >  1:236641/1‑133 (MQ=255)                                                   cAATCAGCGTCGCGTTTTTCTCTTTTGCAGAGGTGCGACCAGCAATATGTTGCAGTACTCGCACTTTTCCCGCTAACTGCGCGCTGTTCCAGGTTTTGTAGCTAAACTGATCTTTATCAAGCACCAACTCGcc                                                                    <  1:411748/133‑1 (MQ=255)                                                   cAATCAGCGTAGCGTATTTCTCTTCTGCAGAGCTGCGACCAACAATATGTTGCAGTACTCGCACTTTTCCCGCTAACTGCGCGCTGTTCCAGGTTTTGTAGCTAAACTGATCTTTATCAAGCACCAACTCGcc                                                                    <  2:195753/133‑1 (MQ=255)                                                       cAGCGTCGCGTTTTTCTCTTTTGCAGAGGTGCGACCAGCAATATGTTGCAGTACTCGCACTTTTCCCGCTAACTGCGCGCTGTTCCAGGTTTTGTAGCTAAACTGATCTTTATCAAGCACCAACTCGCCCCGa                                                                <  1:362179/133‑1 (MQ=255)                                                             cgcgTTTTTCTCTTTTGCAGAGGTGCGACCAGCAATATGTTGCAGTACTCGCTCTTTTCCCGCTAACTGCGCGCTGTTCCAGGTTTTGTAGCTAAACTGATCTTTATCAAGCGCCAACTCGCCCCGATCGGtc                                                          <  2:160527/133‑2 (MQ=255)                                                               cgTTTTTCTCTTTTGCAGAGGTGCGACCAGCAATATGTTGCAGTACTCGCACTTTTCCCGCTAACTGCGCGCTGTTCCAGGTTTTGTAGCTAAACTGATCTTTATCAAGCACCAACTCGCCCCGATCGGTAAt                                                        >  1:19366/1‑133 (MQ=255)                                                                       tctTTTGCAGAGGTGCGACCAGCAATATGTTGCCGTACTCGCACTTTTCCCGCTAACTGCGCGCTGTTCCAGGTTTTGTAGCTAAACTGATCTTTATCAAGCACCAACTCGCCCCGATCGGTAATGCCAATcg                                                <  2:413981/133‑1 (MQ=255)                                                                           ttGCAGAGGTGCGACCAGCAATATGTTGCAGTACTCGCACTTTTCCCGCTAACTGCGCGCTGTTCCAGGTTTTGTAGCTAAACTGATCTTTATCAAGCACAAACTCACACCGATCGGAAATGCCAATcggcgg                                            >  2:275773/1‑133 (MQ=255)                                                                                      cGACCAGCAATATGTTGCAGTACTCGCACTTTTCCCGCTAACTGCGCGCTGTTCCAGGTTTTGTAGCTAAACTGATCTTTATCAAGCACCAACTCGCCCCGATCGGTAATGCCAATCGGCGGCACTCGCTGAc                                 >  1:366743/1‑133 (MQ=255)                                                                                      cGACCAGCAATATGTTGCAGTACTCGCACTTTTCCCGCTAACTGCGCGCTGTTCCAGGTTTTGTAGCTAAACTGATCTTTATCAAGCACCAACTCGCCCCGATCGGTAATGCCAATCGGCGGCACTCGCTGAc                                 <  1:279571/133‑1 (MQ=255)                                                                                      cGACCAGCAATATGTTGCAGTACTCGCACTTTTCCCGCTAACTGCGCGCTGTTCCAGGTTTTGTAGCTAAACTGATCTTTATCAAGCACCAACTCGCCCCGATCGGTAATACCAGTCAGCGGCAGTCGCTGAc                                 >  1:300802/1‑133 (MQ=255)                                                                                      cGACCAGCAATATGTTGCAGTACTCGCACTTTTCCCGCTAACTGCGCGCTGTTCCAGGTTTTGTAGCTAAACTGATCTTTATCAAGCACCAACTCGCCCCAATCGGAAATGCCAATCAGCGACACTCGCTGAc                                 >  2:279884/1‑133 (MQ=255)                                                                                           aGCAATATGTTGCAGTACTCGCACTTTTCCCGCTAACTGCGCGCTGTTCCAGGTTTTGTAGCTAAACTGATCTTTATCAAGCACCAACTCGCCCCGATCGATAACGCCAATCGGCGGCACTCCATGACCGGtt                            >  2:279571/1‑133 (MQ=255)                                                                                             cAATATGTTGCAGTACTCGCACTTTTCCCGCTAACTGCGCGCTGTTCCAGGTTTTGTAGCTAAACTGATCTTTATCAAGCACCAACTCGCCTCGATCGGTAATGCCATACGGCGGCAGTCGCCGACCGGCTTc                          >  2:264914/1‑133 (MQ=255)                                                                                             cAATATGTTGCAGTACTCGCACTTTTCCCGCTAACTGCGCGCTGTTCCAGGTTTTGTAGCTAAACTGATCTTTATCAAGCACCAACTCGCCCCGATCGGTCATGCCAAACGGCGGCACTCGCAGACCGGTTTc                          >  2:261209/1‑133 (MQ=255)                                                                                                      gCAGTACTCGCACTTTTCCCGCTAACTGCGCGCTGTTCCAGGTTTTGTAGCTAAACTGATCTTTATCAAGCACCAACTCGCCCCGATCGGTAATGCCAATCGGCGGCACTCGCTGACCGGTTTCGAACTGAtg                 >  2:39029/1‑133 (MQ=255)                                                                                                        aGTACTCGCACTTTTCCCGCTAACTGCGCGCTGTTCCAGGTTTTGTAGCTAAACTGATCTTTATCAAGCACCAACTCGCCCCGATCGGTAATGCCAATCGGCGGCACTCGCTGACCGGTTTCGAACTGAtgtg               <  1:554225/133‑1 (MQ=255)                                                                                                          tACTCGCACTTTTCCCGCTAACTGCGCGCTGTTCCAGGTTTTGTAGCTAAACTGATCTTTATCAAGCACCAACTCGCCCCGATCGGTAATGCCAATCGGCGGTACTCGCTGACCTGTGTCGAACGGATGTGCg             >  1:190043/1‑133 (MQ=255)                                                                                                          tACTCGCACTTTTCCCGCTAACTGCGCGCTGTTCCAGGTTTTGTAGCTAAACTGATCTTTATCAAGCACCAACTCGCCCCGATCGGTAATGCCAATCGGCGGCACTCGCTGACCGGTTTCGAACTGATGTGCg             >  2:356216/1‑133 (MQ=255)                                                                                                          tACTCGCACTTTTCCCGCTAACTGCGCGCTGTTCCAGGTTTTGTAGCTAAACTGATCTTTATCAAGCACCAACTCGCCCCGATCGGTAATGCCAATCGGCGGCACTCGCTGACCGGTTTCGAACTGATGTGCg             >  1:414336/1‑133 (MQ=255)                                                                                                           aCTCGCACTTTTCCCGCTAACTGCGCGCTGTTCCAGGTTTTGTAGCTAAACTGATCTTTATCAAGCACCAACTCGCCCCGATCGGTAATGCCAATCGGCGGCACTCGCTGACCGGTTTCGAACTGATGTGCgg            <  2:93994/133‑1 (MQ=255)                                                                                                            cTCGCACTTTTCCCGCTAACTGCGCGCTGTTCCAGGTTTTGTAGCTAAACTGATCTTTATCAAGCACCAACTCGCCCCGATCGGTAATGCCAATCGGCGGCACTCGCTGACCGGTTTCGAACTGATGTGCGGa           >  2:417608/1‑133 (MQ=255)                                                                                                                 aCTTTTCCCGCTAACTGCGCGCTGTTCCAGGTTTTGTAGCTAAACTGATCTTTATCAAGCACCAACTCGCCCCGATCGGTAATGCCAATCGGCGGCACTCGCTGACCGGTTTCGAACTGATGTGCGGAAGCca      <  2:414336/133‑1 (MQ=255)                                                                                                                     ttCCCGCTAACTGCGCGCTGTTCCAGGTTTTGTAGCTAAACTGATCTTTATCAAGCACCAACTCGCCCCGATCGGTAATGCCAATCGGCGGCACTCGCTGACCGGTTTCGAACTGATGTGCGGAAGCcatcat  >  1:348851/1‑133 (MQ=255)                                                                                                                          |                                                                                                                                GGTGGTGGTCTGGTAACGATCGTGCGGTAACTTCGCTGATTTAATCGCTTCAATCAGCGTCGCGTTTTTCTCTTTTGCAGAGGTGCGACCAGCAATATGTTGCAGTACTCGCACTTTTCCCACTAACTGCGCGCTGTTCCAGGTTTTGTAGCTAAACTGATCTTTATCAAGCACCAACTCGCCCCGATCGGTAATGCCAATCGGCGGCACTCGCTGACCGGTTTCGAACTGATGTGCGGAAGCCATCAT  >  NC_000913/4438969‑4439217

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑