Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	486,866	G→A	A111T (GCA→ACA)	mscK →	potassium dependent, small conductance mechanosensitive channel

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	486,866	0	G	A	100.0%	100.4 / NA	31	A111T (GCA→ACA)	mscK	potassium dependent, small conductance mechanosensitive channel
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (16/15);  total (16/15)

ATACATTAGCCACCCTCGATAAAATCGATCGCATAAAAGAAGAGACAGTTCAGCTACGGCAAAAAGTCGCTGAAGCGCCGGAAAAAATGCGCCAGGCGACCGCGGCGTTAACAGCACTTAGCGATGTCGATAACGACGAAGAAACGCGCAAAATTCTGAGCACGCTGTCGTTGCGCCAGCTGGAAACTCGCGTTGCCCAGGCGCTGGACGATTTGCAAAACGCACAAAACGATCTGG  >  NC_000913/486753‑486989                                                                                                                  |                                                                                                                            aTACATTAGCCACCCTCGATAAAATCGATCGCATAAAAGAAGAGACAGTTCAGCTATGGCAAAAAGTCGCTGAAGCGCCGGAAAAAATGCGCCAGGCGACCGCGGCGTTAACAACACTTAGCGATGTCGATaa                                                                                                          >  1:556332/1‑133 (MQ=255) aTACATTAGCCACCCTCGATAAAATCGATCGCATAAAAGAAGAGACAGTTCAGCTACGGCAAAAAGTCGCTGAAGCGCCGGAAAAAATGCGCCAGGCGACCGCGGCGTTAACAACACTTAGCGATGTCGAt                                                                                                            <  2:416534/131‑1 (MQ=255) aTACATTAGCCACCCTCGATAAAATCGATCGCATAAAAGAAGAGACAGTTCAGCTACGGCAAAAAGTCGCTGAAGCGCCGGAAAAAATGCGCCAGGCGACCGCGGCGTTAACAACACTTAGCGATGTCGAt                                                                                                            >  1:416534/1‑131 (MQ=255) aTACATTAGCCACCCTCGATAAAATCGATCGCATAAAAGAAGAGACAGTTCAGCTACGGCAAAAAGTCGCTGAAGCGCCGGAAAAAATGCGCCAGGCGACCGCGGCGTTAACAACACTTAGCGATGTCGATaa                                                                                                          <  2:103870/133‑1 (MQ=255) aTACATTAGCCACCCTCGATAAAATCGATCGCATAAAAGAAGAGACAGTTCAGCTACGGCAAAAAGTCGCTGAAGCGCCGGAAAAAATGCGCCAGGCGACCGCGGCGTTAACAACACTTAGCGATGTCGATaa                                                                                                          >  1:440709/1‑133 (MQ=255) aTACATTAGCCACCCTCGATAAAATCGATCGCATAAAAGAAGAGACAGTTCAGCTACGGCAAAAAGTCGCTGAAGCGCCGGAAAAAATGCGCCAGGCGACCGCGGCGTTAACAACACTTAGCGATGTCGATaa                                                                                                          >  2:127152/1‑133 (MQ=255)           cACCCTCGATAAAATCGATCGCATAAAAGAAGAGACAGTTCAGCTACGGCAAAAAGTCGCTGAAGCGCCGGAAAAAATGCGCCAGGCGACCGCGGCGTTAACAACACTTAGCGATGTCGATAACGACgaagaa                                                                                                >  1:537669/1‑133 (MQ=255)               cTCGATAAAATCGATCGCATAAAAGAAGAGACAGTTCAGCTACGGCAAAAAGTCGCTGAAGCGCCGGAAAAAATGCGCCAGGCGACCGCGGCGTTAACAACACTTAGCGATGTCGATAACGACGAAGAAAcgc                                                                                            >  2:333720/1‑133 (MQ=255)               cTCGATAAAATCGATCGCATAAAAGAAGAGACAGTTCAGCTACGGCAAAAAGTCGCTGAAGCGCCGGAAAAAATGCGCCAGGCGACCGCGGCGTTAACAACACTTAGCGATGTCGATAACGACGAAGAAAcgc                                                                                            >  1:594039/1‑133 (MQ=255)                        atcgatcgCATAAAAGAAGAGACAGTTCAGCTACGGCAAAAAGTCGCTGAAGCGCCGGAAAAAATGCGCCAGGCGACCGCGGCGTTAACAACACTTAGCGATGTCGATAACGACGAAGAAACGCGCAAAATTc                                                                                   >  1:584199/1‑133 (MQ=255)                            atcgCATAAAAGAAGAGACAGTTCAGCTACGGCAAAAAGTCGCTGAAGCGCCGGAAAAAATGCGCCAGGCGACCGCGGCGTTAACAACACTTAGCGATGTCGTTAACGACGAAGCAATGCGCAATATTCTGAg                                                                               >  1:295934/1‑133 (MQ=255)                            atcgCATAAAAGAAGAGACAGTTCAGCTACGGCAAAAAGTCGCTGAAGCGCCGGAAAAAATGCGCCAGGCGACCGCGGCGTTAACAACACTTAGCGATGTCGATAACGACGAAGAAACGCGCAAAATTCTGAg                                                                               <  2:144116/133‑1 (MQ=255)                            atcgCATAAAAGAAGAGACAGTTCAGCTACGGCAAAAAGTCGCTGAAGCGCCGGAAAAAATGCGCCAGGCGACCGCGGCGTTAACAACACTTAGCGATGTCGATAACGACGAAGAAACGCGCAAAATTCTGAg                                                                               >  2:467767/1‑133 (MQ=255)                             tcgCATAAAAGAAGAGACAGTTCAGCTACGGCAAAAAGTCGCTGAAGCGCCGGAAAAAATGCGCCAGGCGACCGCGGCGTTAACAACACTTAGCGATGTCGATAACGACGAAGAAACGCGCAAAATTCTGAg                                                                               >  1:545474/1‑132 (MQ=255)                             tcgCATAAAAGAAGAGACAGTTCAGCTACGGCAAAAAGTCGCTGAAGCGCCGGAAAAAATGCGCCAGGCGACCGCGGCGTTAACAACACTTAGCGATGTCGATAACGACGAAGAAACGCGCAAAATTCTGAg                                                                               <  2:545474/132‑1 (MQ=255)                              cgCATAAACGAAGAGTCACTTCAGCTACGGTAAAAAGTCGCTGAAGCGCCGGAAAAAATGCGCCAGGCGACCGCGGCGTTAACAACACTTAGCGATGTCGATAACGACGAAGAAACGCGCAAAATTCTGAGCa                                                                             <  1:294638/133‑1 (MQ=255)                              cgCATAAAAGAAGAGACAGTTCAGCTACGGCAAAAAGTCGCTGAAGCGCCGGAAAAAATGCGCCAGGCGACCGCGGCGTTAACAACACTTAGCGATGTCGATAACGACGAAGAAACGCGCAAAATTCTGAGCa                                                                             <  1:253277/133‑1 (MQ=255)                                           agaCAGTTCAGCTACGGCAAAAAGTCGCTGAAGCGCCGGAAAAAATGCGCCAGGCGACCGCGGCGTTAACAACACTTAGCGATGTCGATAACGACGAAGAAACGCGCAAAATTCTGAGCACGCTGTCGTTgcg                                                                >  1:498478/1‑133 (MQ=255)                                                 ttCAGCTACGGCAAAAAGTCGCTGAAGCGCCGGAAAAAATGCGCCAGGCGACCGCGGCGTTAACAACACTTAGCGATGTCGATAACGACGAAGAAACGCGCAAAATTCTGAGCACGCTGTCGTTGCGCCAGCt                                                          >  1:457386/1‑133 (MQ=255)                                                  tCAGCTACGGCAAAAAGTCGCTGAAGCGCCGGAAAAAATGCGCCAGGCGACCGCGGCGTTAACAACACTTAGCGATGTCGATAACGACGAAGAAACGCGCAAAATTCTGAGCACGCTGTCGTTGCGCCAGCTg                                                         >  1:362172/1‑133 (MQ=255)                                                       tACGGCAAAAAGTCGCTGAAGCGCCGGAAAAAATGCGCCAGGCGACCGCGGCGTTAACAACACTTAGCGATGTCGATAACGACGAAGAAACGCGCAAAATTCTGAGCACGCTGTCGTTGCGCCAGCTGGAAAc                                                    <  2:498478/133‑1 (MQ=255)                                                          ggCAAAAAGTCGCTGAAGCGCCGGAAAAAATGCGCCAGGCGACCGCGGCGTTAACAACACTTAGCGATGTCGATAACGACGAAGAAACGCGCAAAATTCTGAGCACGCTGTCGTTGCGCCAGCTGGAAACTcg                                                 <  1:333720/133‑1 (MQ=255)                                                           gCAAAAAGTCGCTGAAGCGCCGGAAAAAATGCGCCAGGCGACCGCGGCGTTAACAACACTTAGCGATGTCGATAACGACGAAGAAACGCGCAAAATTCTGAGCACGCTGTCGTTGCGCCAGCTGGAAACTcgc                                                >  2:321257/1‑133 (MQ=255)                                                           gCAAAAAGTCGCTGAAGCGCCGGAAAAAATGCGCCAGGCGACCGCGGCGTTAACAACACTTAGCGATGTCGATAACGACGAAGAAACGCGCAAAATTCTGAGCACGCTGTCGTTGCGCCAGCTGGAAACTcgc                                                >  2:460180/1‑133 (MQ=255)                                                             aaaaaGTCGCTGAAGCGCCGGAAAAAATGCGCCAGGCGACCGCGGCGTTAACAACACTTAGCGATGTCGATAACGACGAAGAAACGCGCAAAATTCTGAGCACGCTGTCGTTGCGCCAGCTGGAAACTCGCGt                                              <  1:127152/133‑1 (MQ=255)                                                                   tCGCTGAAGCGCCGGAAAAAATGCGCCAGGCGACCGCGGCGTTAACAACACTTAGCGATGTCGATAACGACGAAGAAACGCGCAAAATTCTGAGCACGCTGTCGTTGCGCCAGCTGGAAACTCGCGTTGCCCa                                        <  2:110100/133‑1 (MQ=255)                                                                                                gCGACCGCGGCGTTAACAACACTTAGCGATGTCGATAACGACGAAGAAACGCGCAAAATTCTGAGCACGCTGTCGTTGCGCCAGCTGGAAACTCGCGTTGCCCAGGCGCTGGACGATTTGCAAAACGCACaaa           <  1:460180/133‑1 (MQ=255)                                                                                                         gcgTTAACAACACTTCGCGATGTCAATAGCGACGAAGAAACTCGCAAAATTCTGAGCACGCTGTCGTTGCGCCAGCTGGAAACTCGCGTTGCCCAGGCGCTGGACGATTTGCAAAACGCACAAAACGATCTgg  <  2:235812/133‑1 (MQ=255)                                                                                                         gcgTTAACAACACTTAGCGATGTCGATAACGACGAAGAAACGCGCAAAATTCTGAGCACGCTGTCGTTGCGCCAGCTGGAAACTCGCGTTGCCCAGGCGCTGGACGATTTGCAAAACGCACAAAACGATCTgg  <  2:362172/133‑1 (MQ=255)                                                                                                         gcgTTAACAACACTTAGCGATGTCGATAACGACGAAGAAACGCGCAAAATTCTGAGCACGCTGTCGTTGCGCCAGCTGGAAACTCGCGTTGCCCAGGCGCTGGACGATTTGCAAAACGCACAAAACGATCTgg  <  2:440709/133‑1 (MQ=255)                                                                                                         gcgTTAACAACACTTAGCGATGTCGATAACGACGAAGAAACGCGCAAAATTCTGAGCACGCTGTCGTTGCGCCAGCTGGAAACTCGCGTTGCCCAGGCGCTGGACGATTTGCAAAACGCACAAAACGATCTgg  <  2:556332/133‑1 (MQ=255)                                                                                                                  |                                                                                                                            ATACATTAGCCACCCTCGATAAAATCGATCGCATAAAAGAAGAGACAGTTCAGCTACGGCAAAAAGTCGCTGAAGCGCCGGAAAAAATGCGCCAGGCGACCGCGGCGTTAACAGCACTTAGCGATGTCGATAACGACGAAGAAACGCGCAAAATTCTGAGCACGCTGTCGTTGCGCCAGCTGGAAACTCGCGTTGCCCAGGCGCTGGACGATTTGCAAAACGCACAAAACGATCTGG  >  NC_000913/486753‑486989

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑