| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,599,347 | A→G | L32S (TTA→TCA) | opgB ← | phosphoglycerol transferase I |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,599,347 | 0 | A | G | 97.4% | 134.3 / NA | 39 | L32S (TTA→TCA) | opgB | phosphoglycerol transferase I |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (20/18); minor base T (1/0); total (21/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CGCCAGCACCGGTCAGGCTGTTGGTTAAGGTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTGATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGCACAGAGGCGAGAAAAAGGGCGAAAGAGAGTAGTTCTGACAACGGGCGATCCTCACTAAACCGGGCCGTTTAGCC > NC_000913/4599225‑4599474 | cGCCAGCACCGGTCAGGCTGTTGGTTAAGGTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTGATATTTAAAACGACAAATAGCCCCAGCACCGTTGACGTGGCTGc < 1:156499/133‑1 (MQ=255) ccGGTCAGGCTGTTGGTTAAGGTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTGATATTTAAAACGACAAATAGCCCCAGCACCGTTGACGTGGCTGCAAaccacc < 2:356916/133‑1 (MQ=255) taGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTGATATTTAAAACGACAAATAGCCCCAGCACCGTTGACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATg > 1:593570/1‑133 (MQ=255) gagaACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTGATATTTAAAACGACAAATAGCCCCAGCACCGTTGACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCg < 2:608439/133‑1 (MQ=255) ccGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTGATATTTAAAACGACAAATAGCCCCAGCACCGTTGACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAAt > 2:983/1‑133 (MQ=255) ccGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTGATATTTAAAACGACAAATAGCCCCAGCACCGTTGACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAAt > 2:465221/1‑133 (MQ=255) ccGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTGATATTTAAAACGACAAATAGCCCCAGCACCGTTGACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAAt > 2:423160/1‑133 (MQ=255) ccGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTGATATTTAAAACGACAAATAGCCCCAGCACCGTTGACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAAt < 1:983/133‑1 (MQ=255) ccGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTGATATTTAAAACGACAAATAGCCCCAGCACCGTTGACGTGGCAGCAAACCACCAGGTGTTGCGTCCCGTTTTCTTTGCGTAAAt > 2:315075/1‑133 (MQ=255) ccGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTGATATTTAAAACGACAAATAGCCCCAGCACCGTTGACGTGGCAGCAAACCAACAGGTGTTACGTCCCGGTTTCTATGCGTAAAt > 2:236495/1‑133 (MQ=255) cgcgTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTGATATTTAAAACGACAAATAGCCCCAGCACCGTTGACGTGGCTTCAAACCACTAGGTGTTTCGTCCCGCGTTCCCTGCGTAAATc > 2:237791/1‑133 (MQ=255) cgcgTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTGATATTTAAAACGACAAATAGCCCCAGCACCGTTGACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATc > 2:457588/1‑133 (MQ=255) cgcgTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTGATATTTAAAACGACAAATAGCCCCAGCACCGTTGACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATc > 2:492550/1‑133 (MQ=255) cgcgTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTGATATTTAAAACGACAAATAGCCCCAGCACCGTTGACGTGGCTACAAACCACCAGGCGTTACGTCCCGCCTTCCATGCGTAAATc > 2:269348/1‑133 (MQ=255) cgtcgtTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTGATATTTAAAACGACAAATAGCCCCAGCACCGTTGACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAg > 1:448089/1‑133 (MQ=255) cgtcgtTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTGATATTTAAAACGACAAATAGCCCCAGCACCGTTGACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAg > 2:477449/1‑133 (MQ=255) gtTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTGATATTTAAAACGACAAATAGCCCCAGCACCGTTGACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGcaca < 1:492550/133‑1 (MQ=255) aaTACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTGATATTTAAAACGACAAATAGCCCCAGCACCGTTGACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGCACagag < 2:127606/133‑1 (MQ=255) aTCGCCAGTAAAATAGTCGCTGGCAAACAGGGTGATATTTAAAACGACAAATAGCCCCAGCACCGTTGACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGca > 1:603647/1‑122 (MQ=255) aTCGCCAGTAAAATAGTCGCTGGCAAACAGGGTGATATTTAAAACGACAAATAGCCCCAGCACCGTTGACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGca < 2:603647/122‑1 (MQ=255) cGCCAGTAAAATAGTCGCTGGCAAACAGGGTGATATTTAAAACGACAAATAGCCCCAGCACCGTTTACGTGGCTGCAAACCACCAGGAGTTACGTCCCGCTTTCCATGCGTAAATCAGCACAGAGGCGAGaaa > 2:637825/1‑133 (MQ=255) cGCCAGTAAAATAGTCGCTGGCAAACAGGGTGATATTTAAAACGACAAATAGCCCCAGCACCGTTGACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGCACAGAGGCGAGaaa > 1:472108/1‑133 (MQ=255) gTAAAATAGTCGCTGTCATACAGGGTGATATTTAAAACGACAAATAGCCCCAGCACCGTTGACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGCACAGAGGCGAGAAAAAggg < 1:237791/133‑1 (MQ=255) gTAAAATAGTCGCTGGCAAACAGGGTGATATTTAAAACGACAAATAGCCCCAGCACCGTTGACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGCACAGAGGCGAGAAAAAggg < 1:625657/133‑1 (MQ=255) gTAAAATAGTCGCTGGCAAACAGGGTGATATTTAAAACGACAAATAGCCCCAGCACCGTTGACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCACGCGTAAATCAGCACAGAGGCGAGAAAAAggg > 1:139547/1‑133 (MQ=255) aTAGTCGCTGGCAAACAGGGTGATATTTAAAACGACAAATAGCCCCAGCACCGTTGACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGCACAGAGGCGAGAAAAAGGGCGaaa < 1:536633/133‑1 (MQ=255) tCGCTGGCAAACAGGGTGATATTTAAAACGACAAATAGCCCCAGCACCGTTGACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGCACAGAGGCGAGAAAAAGGGCGAAagaga > 2:26912/1‑133 (MQ=255) cGCTGGCAAACAGGGTGATATTTAAAACGACAAATAGCCCCAGCACCGTTGACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGCACAGAGGCGAGAAAAAGGGCGAAagagag < 2:472108/133‑1 (MQ=255) gCAAACAGGGTGATATTTAAAACGACAAATAGCCCCAGCACCGTTGACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGCACAGAGGCGAGAAAAAGGGCGAAAGAGAGTAGtt < 2:448089/133‑1 (MQ=255) ggTGATATTTAAAACGACAAATAGCCCCAGCACCGTTGACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGCACAGAGGCGAGAAGAAGGGCGAAAGAGAGTAGTGCTGACAAc > 1:241096/1‑133 (MQ=255) gATATTTAAAACGACAAATAGCCCCAGCACCGTTGACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGCACAGAGGCGAGAAAAAGGGCGAAAGAGAGTAGTTCTGACAACggg < 1:465221/133‑1 (MQ=255) tttAAAACTACAAATTGCTCCACCACCGTTGACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGCACAGAGGCGAGAAAAAGGGCGAAAGAGAGTAGTTCTGACAACGGGCGAt < 1:269348/133‑1 (MQ=255) tttAAAACGACAAATAGCCCCAGCACCGTTGACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGCACAGAGGCGAGAAAAAGGGCGAAAGAGAGTAGTTCTGACAACGGGCGAt < 1:26912/133‑1 (MQ=255) ttAAAACGACAAATAGCCCCAGCACCGTTGACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGCACAGAGGCGAGAAAAAGGGCGAAAGAGAGTAGTTCTGACAACGGGCGATc < 1:423160/133‑1 (MQ=255) aaaaCGACAAATAGCCCCAGCACCGTTGACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGCACAGAGGCGAGAAAAAGGGCGAAAGAGAGTAGTTCTGACAACGGGCGATCCt > 1:25060/1‑133 (MQ=255) aaCGACAAATAGCCCCAGCACCGTTGACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGCACAGAGGCGAGAAAAAGGGCGCAAGAGAGAAGTTCTGATAACGGGCGATCCTCa > 2:237395/1‑133 (MQ=255) aGCACCGTTGACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGCACAGAGGCGAGAAAAAGGGCGAAAGAGAGTAGTTCTGACAACGGGCGATCCTCACTAAACCGGGCCGttt > 2:22317/1‑133 (MQ=255) ccGTTGACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGCACAGAGGCGAGAAAAAGGGCGAAAGAGAGTAGTTCTGACAACGGGCGATCCTCACTAAACCGGGCCGTTTAGcc < 1:457588/133‑1 (MQ=255) ccGTTGACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCACGCGTAAATCAGCACAGAGGCGAGAAAAAGGGCGAAAGAGAGTAGTTCTGACAACGGGCGATCCTCACTAAACCGGGCCGTTTAGcc < 2:139547/133‑1 (MQ=255) | CGCCAGCACCGGTCAGGCTGTTGGTTAAGGTATAGAGAACCGCGTCGTTAATACCATCGCCAGTAAAATAGTCGCTGGCAAACAGGGTGATATTTAAAACGACAAATAGCCCCAGCACCGTTAACGTGGCTGCAAACCACCAGGTGTTACGTCCCGCTTTCCATGCGTAAATCAGCACAGAGGCGAGAAAAAGGGCGAAAGAGAGTAGTTCTGACAACGGGCGATCCTCACTAAACCGGGCCGTTTAGCC > NC_000913/4599225‑4599474 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |