Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,630,832	C→T	R34C (CGT→TGT)	slt →	soluble lytic murein transglycosylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,630,832	0	C	T	100.0%	142.0 / NA	42	R34C (CGT→TGT)	slt	soluble lytic murein transglycosylase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (23/19);  total (23/19)

ATGTATTTACACTTAGAGGATGCGCTTGTGGAAAAAGCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGTCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAGGATTATCCGCTTTATCCCTACCT  >  NC_000913/4630706‑4630935                                                                                                                               |                                                                                                        aTGTATTTACACTTAGAGGATGCGCTTGTGGAAAAAGCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGTGTAGTc                                                                                                   >  2:99965/1‑133 (MQ=255) aTGTATTTACACTTAGAGGATGCGCTTGTGGAAAAAGCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCACCAGCGTGGAGCGAGCCGACTCACTGCATGACCAGTGTAGTc                                                                                                   >  2:187385/1‑133 (MQ=255)      tttACACTTAGAGGATGCGCTTGTGGAAAAAGCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGTGTAGTCGTTAc                                                                                              >  2:496189/1‑133 (MQ=255)         acacTTAGAGGATGCGCTTGTGGAAAAAGCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGTGTAGTCGTTAcgcg                                                                                           >  1:109258/1‑133 (MQ=255)           acTTAGAGGATGCGCTTGTGGAAAAAGCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGTGTAGTCGTTACGCGCa                                                                                         >  1:630101/1‑133 (MQ=255)                gagGATGCGCTTGTGGAAAAAGCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGTGTAGTCGTTACGCGCAAATCa                                                                                    >  2:58537/1‑133 (MQ=255)                  ggATGCGCTTGTGGAAAAAGCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGTGTAGTCGTTACGCGCAAATCAAg                                                                                  <  2:630101/133‑1 (MQ=255)                  ggATGCGCTTGTGGAAAAAGCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGTGTAGTCGTTACGCGCAAATCAAg                                                                                  <  2:460765/133‑1 (MQ=255)                  ggATGCGCTTGTGGAAAAAGCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGTGTAGTCGTTACGCGCAAATCAAg                                                                                  <  1:54053/133‑1 (MQ=255)                     tGCGCTTGTGGAAAAAGCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGTGTAGTCGTTACGCGCAAATCAAg                                                                                  <  2:462883/130‑1 (MQ=255)                     tGCGCTTGTGGAAAAAGCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGTGTAGTCGTTACGCGCAAATCAAg                                                                                  >  1:408071/1‑130 (MQ=255)                     tGCGCTTGTGGAAAAAGCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGTGTAGTCGTTACGCGCAAATCAAg                                                                                  <  2:408071/130‑1 (MQ=255)                     tGCGCTTGTGGAAAAAGCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGTGTAGTCGTTACGCGCAAATCAAg                                                                                  >  1:462883/1‑130 (MQ=255)                     tGCGCTTGTGGAAAAAGCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGTGTAGTCGTTACGCGCAAATCAAGCAg                                                                               >  1:157576/1‑133 (MQ=255)                     tGCGCTTGTGGAAAAAGCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGTGTAGTCGTTACGCGCAAATCAAGCAg                                                                               >  2:344434/1‑133 (MQ=255)                        gcTTGTGGAAAAAGCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGTGTAGTCGTTACGCGCAAATCAAGCAGGcc                                                                            <  1:344434/133‑1 (MQ=255)                           tgtgGAAAAAGCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGTGTAGTCGTTACGCGCAAATCAAGCAGGCCTgg                                                                         >  2:47439/1‑133 (MQ=255)                             tgGAAAAAGCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGTGTAGTCGTTACGCGCAAATCAAGCAGGCCTGGGa                                                                       >  2:409489/1‑133 (MQ=255)                                               ttACCTGGCGGCTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGTGTAGTCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGAtgt                                                     >  2:53437/1‑133 (MQ=255)                                                tACCTGGCGGCTGTTGGCTGCCGGTGTCTGTCTGCGGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGTGTAGTCGTTACACGCAAATCGAGCCGCCCTGGGATAATCGACTAATGGAtgag                                                    >  2:303625/1‑131 (MQ=255)                                                    tGGCGGCTTTTGGCTGCCGGTGTCTGTCTGTTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGTGTAGTCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCg                                                <  1:310315/133‑1 (MQ=255)                                                    tGGCGGCTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGTGTAGTCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCg                                                <  2:571828/133‑1 (MQ=255)                                                      gcggcTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGTGTAGTCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGaa                                              <  1:58537/133‑1 (MQ=255)                                                           tGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGTGTAGTCGTTACGCGCAAATCAATCAGGCCTGTGATAATCAACAAATGGGTGTGGTCGAACAAat                                         >  2:165305/1‑133 (MQ=255)                                                           tGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGTGTAGTCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAat                                         <  1:496189/133‑1 (MQ=255)                                                           tGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGTGTAGTCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAat                                         <  2:109258/133‑1 (MQ=255)                                                           tGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGTGTAGTCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAat                                         <  2:157576/133‑1 (MQ=255)                                                                   gCCGGTGTCTGTCTGCTGCCGGACAGCAGCCTGGCGCGAGCCGACTCACTGGATGAGCAGTGTAGTCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTg                                 <  1:165305/133‑1 (MQ=255)                                                                        tgtctgtcTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGTGTAGTCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTg                            <  1:409489/133‑1 (MQ=255)                                                                        tgtctgtcTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGTGTAGTCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTg                            <  1:517371/133‑1 (MQ=255)                                                                              tctgCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGTGTAGTCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAGGAt                      >  2:148307/1‑133 (MQ=255)                                                                                  ctgACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGTGTAGTCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAAAGGATGTGGGCGACCAAATGATGCCTAGACTCAAGGATTATc                  >  2:257330/1‑133 (MQ=255)                                                                                   tgACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGTGTAGTCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAGGATTATcc                 <  1:99965/133‑1 (MQ=255)                                                                                        gTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGTGTAGTCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAGGATTATCCGCttt            >  2:412216/1‑133 (MQ=255)                                                                                        gTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGTATAGTCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAATTGATGCCGGGAATGGAGGATTGTCCGCttt            >  1:188816/1‑133 (MQ=255)                                                                                          cagcagCGTGGCGCGAGCCGACTCACTGGATGAGCAGTGTAGTCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAGGATTATCCGCTTTAt          >  1:414960/1‑133 (MQ=255)                                                                                           agcagcGTGGCGCGAGCCGACTCACTGGATGAGCAGTGTAGTCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAGGATTATCCGCTTTATc         <  2:414960/133‑1 (MQ=255)                                                                                           agcagcGTGGCGCGAGCCGACTCACTGGATGAGCAGTGTAGTCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAGGATTATCCGCTTTATc         >  1:406486/1‑133 (MQ=255)                                                                                           agcagcGTGGCGCGAGCCGACTCACTGGATGAGCAGTGTAGTCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAGGATTATCCGCTTTATc         <  1:47439/133‑1 (MQ=255)                                                                                              agcGTGGCGCGAGCCGACTCACTGGATGAGCAGTGTAGTCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAGGATTATCCGCTTTATCCCt      <  1:605871/133‑1 (MQ=255)                                                                                               gcGTGGCGCGAGCCGACTCACTGGATGAGCAGTGTAGTCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGGAGGATTTTCCGCTATATCCCTa     >  1:218141/1‑133 (MQ=255)                                                                                                  tGGCGCGAGCCGACTCACTGGATGAGCAGTGTAGTCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAGGATTATCCGCTTTATCCCTACCt  >  1:487307/1‑133 (MQ=255)                                                                                                                               |                                                                                                        ATGTATTTACACTTAGAGGATGCGCTTGTGGAAAAAGCCAAACAAGTTACCTGGCGGCTGTTGGCTGCCGGTGTCTGTCTGCTGACGGTCAGCAGCGTGGCGCGAGCCGACTCACTGGATGAGCAGCGTAGTCGTTACGCGCAAATCAAGCAGGCCTGGGATAATCGACAAATGGATGTGGTCGAACAAATGATGCCTGGACTGAAGGATTATCCGCTTTATCCCTACCT  >  NC_000913/4630706‑4630935

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑