Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	23,401	C→T	G337G (GGC→GGT)	ileS →	isoleucine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	23,401	0	C	T	100.0%	92.9 / NA	28	G337G (GGC→GGT)	ileS	isoleucine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (10/18);  total (10/18)

GGTAAAAGGTGCGGAGCTTGAGCTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGCTGGATGGCGTGAACGTCTTCAAAGCGAAC  >  NC_000913/23275‑23530                                                                                                                               |                                                                                                                                  ggTAAAAGGTGCGGAGCTTGAGCTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGTCACGGc                                                                                                                             <  2:260090/133‑1 (MQ=255)   tAAAAGGTGCGGAGCTTGAGCTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGTCACGGccc                                                                                                                           <  2:556975/133‑1 (MQ=255)   tAAAAGGTGCGGAGCTTGAGCTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGTCACGGccc                                                                                                                           <  2:318561/133‑1 (MQ=255)       aGGTGCGGCGCTTGATCTCCTGCGCTTTACTCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGTCACGGCCCGgac                                                                                                                       <  1:169617/133‑1 (MQ=255)       aGGTGCGGCGCTTGACCTGCTGAGCTTTACACATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGTCACGGCCCGgac                                                                                                                       <  1:231265/133‑1 (MQ=255)       aGGTGCGGAGCTTGAGCTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGTCACGGCCCGgac                                                                                                                       <  2:49814/133‑1 (MQ=255)       aGGTGCGGAGCTTGACCTCCTGTGCTTTACGCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGTCACGGCCCGgac                                                                                                                       <  1:310184/133‑1 (MQ=255)           gCGGAGCTTGAGCTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGTCACGGCCCGGACGACt                                                                                                                   >  2:540036/1‑133 (MQ=255)                  ttGAGCTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGTCACGGCCCGGACGACTATGTGAt                                                                                                            <  1:429568/133‑1 (MQ=255)                                  tACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGTCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGc                                                                                            <  1:473933/133‑1 (MQ=255)                                    cccATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGTCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCt                                                                                          >  2:317047/1‑133 (MQ=255)                                    cccATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGTCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCt                                                                                          >  2:28548/1‑133 (MQ=255)                                       aTCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGTCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGa                                                                                       <  2:97929/133‑1 (MQ=255)                                       aTCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGTCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGa                                                                                       >  1:109702/1‑133 (MQ=255)                                             ttATGGGCTCTGACGTTCCGGCAATCTTCGGCTATCACGTTCCCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGTCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGc                                                                                 <  2:312013/133‑1 (MQ=255)                                                   gCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGTCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAAccc                                                                           >  1:643027/1‑133 (MQ=255)                                                      tCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGTCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGt                                                                        >  2:628876/1‑133 (MQ=255)                                                             tCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGTCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACAGCTAACCCGGTTGGCCCg                                                                 >  1:277972/1‑133 (MQ=255)                                                               cGGCAATCCTCGGCGTTCACGTTACCCTGGATCCCGGTACCCGTGCCGTTCACACCGCGCCTGGTCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGa                                                               <  2:183445/133‑1 (MQ=255)                                                               cGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGTCACGGCCCGGACGACTATGTGATCGGTCAGAAATATGGCCTGGAAACCGCTAACCCGGTTGGCCCGGa                                                               <  2:532530/133‑1 (MQ=255)                                                               cGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGTCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGa                                                               <  2:109702/133‑1 (MQ=255)                                                                  cAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGTCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACgg                                                            <  1:540036/133‑1 (MQ=255)                                                                       cTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGTCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTGACCCGGTTGGCCCGGACGGCACtt                                                       >  2:218233/1‑133 (MQ=255)                                                                               tCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGTCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCg                                               >  1:574811/1‑133 (MQ=255)                                                                                                            ccGTTCACACCGCGCCTGGTCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGCTGGATGGCGTGaa                  <  1:317047/133‑1 (MQ=255)                                                                                                              gTTCACACCGCGCCTGGTCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGCTGGATGGCGTGAACg                >  1:318677/1‑133 (MQ=255)                                                                                                                    acCGCGCCTGGTCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGCTGGATGGCGTGAACGTCTTCa          <  2:643027/133‑1 (MQ=255)                                                                                                                            tGGTCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCAAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGCTGGATGGCGTGAACGTCTTCAAAGCGAAc  <  2:487606/133‑1 (MQ=255)                                                                                                                               |                                                                                                                                  GGTAAAAGGTGCGGAGCTTGAGCTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGCTGGATGGCGTGAACGTCTTCAAAGCGAAC  >  NC_000913/23275‑23530

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑