Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	616,577	T→A	I807I (ATT→ATA)	entF →	apo‑serine activating enzyme

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	616,577	0	T	A	100.0%	56.7 / NA	18	I807I (ATT→ATA)	entF	apo‑serine activating enzyme
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (5/13);  total (5/13)

CTGGCACAGGTGCGCGGCAGCAGTGTGCCGATTGGTTATCCGGTATGGAATACGGGTCTGCGTATTCTTGATGCGATGATGCATCCGGTGCCGCCGGGTGTGGCGGGTGATCTCTATCTCACTGGCATTCAACTGGCGCAGGGCTATCTCGGACGCCCCGATCTGACCGCCAGCCGCTTTATTGCCGATCCTTTTGCCCCAGGTGAACGGATGTACCGTACCGGAGACGTTGCCCGCTGGCTGGATAACGGCGCGGT  >  NC_000913/616449‑616705                                                                                                                                 |                                                                                                                                 cTGGCACAGGTGCGCGGCAGCAGTGTGCCGATTGGTTATCCGGTATGGAATACGGGTCTGCGTATTCTTGATGCGATGATGCATCCGGTGCCGCCGGGTGTGGCGGGTGATCTCTATCTCACTGGCATACAAc                                                                                                                              <  2:14390/133‑1 (MQ=255)      acaGGTGCGCGGCAGCAGTGTGCTGATAGGTTATCCGGTATGGAATACGGGTCTGCGTATTCTTGATGCGATGATGCATCCGGTGCCGCCGGGTGTGGCGGGTGATCTCTATCTCACTGGCATACAACTGgcg                                                                                                                         <  1:399787/133‑1 (MQ=255)      acaGGTGCGCGGCAGCAGTGTGCCGATTGGTTATCCGGTATGGAATACGGGTCTGCGTATTCTTGATGCGATGATGCATCCGGTGCCGCCGGGTGTGGCGGGTGATCTCTATCTCACTGGCATACAACTGgcg                                                                                                                         <  2:417734/133‑1 (MQ=255)         ggTGCGCGGCAGCAGTGTGCCGATTGGTTATCCGGTATGGAATACGGGTCTGCGTATTCTTGATGCGATGATGCATCCGGTGCCGCCGGGTGTGGCGGGGGGTCTTTTTCTTACTCGCGTACAACTGgcggcg                                                                                                                      >  2:526811/1‑130 (MQ=255)          gTGCGCGGCAGCAGTGTGCCGATTGGTTATCCGGTATGGAATACGGGTCTGCGTATTCTTGATGCGATGATGCATCCGGTGCCGCCGGGTGTGGCGGGTGATCTCTATCTCACTGGCATACAACTGGCGCAgg                                                                                                                     <  2:504162/133‑1 (MQ=255)           tGCGCGGCAGCAGTGTGCCGATTGGTTATCCGGTATGGAATACGGGTCTGCGTATTCTTGATGCGATGATGCATCCGGTGCCGCCGGGTGTGGCGGGTGATCTCTATCTCACTGGCATACAACTGGCGCAggg                                                                                                                    <  1:376826/133‑1 (MQ=255)                               aTTGGTTATCCGGTATGGAATACGGGTCTGCGTATTCTTGATGCGATGATGCATCCGGTGCCGCCGGGTGTGGCGGGTGATCTCTATTTCACTGGCATACAAATGGCGCCGGGCCATCTCCGACGcccccctc                                                                                                >  1:109074/1‑129 (MQ=255)                               aTTGGTTATCCGGTATGGAATACGGGTCTGCGTATTCTTGATGCGATGATGCATCCGGTGCCGCCGGGTGTGGCGGGGGAACTCTCTTTCCCTGGGATAAAAATCGCGCCCGGCTGTCTCGGGCGCCCCGCTc                                                                                                >  1:318517/1‑133 (MQ=255)                                         cGGTATGGAATACGGGTCTGCGTATTCTTGATGCGATGATGCATCCGGTGCCGCCGGGTGTGGCGGGTGATCTCTATCTCACTGGCATACAACTGGCGCAGGGCTATCTCGGACGCCCCGATCTGACCGCCAg                                                                                      <  1:325124/133‑1 (MQ=255)                                          ggTATGGAATACGGGTCTGCGTATTCTTGATGCGATGATGCATCCGGTGCCGCCGGGTGTGGCGGGTGATCTCTATCTCACTGGCATACAACTGGCGCAGGGCTATCTCGGACGCCCCGATCTGACCGCCAGc                                                                                     <  2:518711/133‑1 (MQ=255)                                                        gTCTGCGTATTCTTGATGCGATGATGCATCCGGTGCCGCCGGGTGTGGCGGGTGATCTCTATCTCACTGGCATACAACTGGCGCAGGGCTATCTCGGACGCCCCGATCTGACCGCCAGCCGCTTTATTGCCGa                                                                       <  1:7444/133‑1 (MQ=255)                                                                aTTCTTGATGCGGTGATGCATCCGGTGCCGCCGGGTGTGGCGGGTGGTCTCTATCTCCCTTGCATACAAAAAGCGCGGGGGGATCTCGGTAGCCCCCCACTGACCCCCCCCCGCCTTTTTTCCGATCCctttt                                                               >  2:613943/1‑132 (MQ=255)                                                                   cTTGATGCGATGATGCATCCGGTGCCGCCGGGTGTGGCGGGTGATCTCTATCTCACTGGCATACAACTGGCGCAGGGCTATCTCGGACGCCCCGATCTGACCGCCAGCCGCTTTATTGCCGATCCTTTTGccc                                                            <  1:509148/133‑1 (MQ=255)                                                                              gatgCATCAGGTGCCTCCAGGTTTGGCGGGGGATCTCTATCTCACTGGCATACAACTGGCGCAGGGCTATCTCGGACGCCCCGATCTGACCGCCAGCCGCTTTATTGCCGATCCTTTTGCCCCAGGTGAACgg                                                 <  1:297361/133‑1 (MQ=255)                                                                                   aTCCGGTGCCGCCGGGTGTGGCGGGTGATCTCTATCTCACTGGCATACAACTGGCGCAGGGCTATCTCGGACGCCCCGATCTGACCGCCAGCCGCTTTATTGCCGATCCTTTTGCCCCAGGTGAACGGATgta                                            <  1:334178/133‑1 (MQ=255)                                                                                                                    aTCTCACTGGCATACAACTGGCGCAGGGCTATCTCGGACGCCCCGATCTGACCGCCAGCCGCTTTATTGCCGATCCTTTTGCCCCAGGTGAACGGATGTACCGTACCGGAGACGTTGCCCGCTGGCTGGATaa           <  1:526811/133‑1 (MQ=255)                                                                                                                     tctcACTGGCATACAACTGGCGCAGGGCTATCTCGGACGCCCCGATCTGACCGCCAGCCGCTTTATTGCCGATCCTTTTGCCCCAGGTGAACGGATGTACCGTACCGGAGACGTTGCCCGCTGGCTGGATAAc          >  1:431603/1‑133 (MQ=255)                                                                                                                        cACTGGCATACAACTGGCGCAGGGCTATCTCGGACGCCCCGATCTGACCGCCAGCCGCTTTATTGCCGATCCTTTTGCCCCAGGTGAACGGATGTACCGTACCGGAGACGTTGCCCGCTTGCTGGATAACGgc       >  2:486003/1‑133 (MQ=255)                                                                                                                           tGGCATACAACTGGCGCAGGGCTATCTCGGACGCCCCGATCTGACCGCCAGCCGCTTTATTGCCGATCCTTTTGCCCCAGGTGAACGGATGTACCGTACCGGAGACGTTGCCCGCTGGCTGGATAACGGcgcg    >  1:472652/1‑133 (MQ=255)                                                                                                                             gCATACAACTGGCGCAGGGCTATCTCGGACGCCCCGATCTGACCGCCAGCCGCTTTATTGCCGATCCTTTTGCCCCAGGTGAACGGATGTACCGTACCGGAGACGTTGCCCGCTGGCTGGATAACGGCGCGGt  >  2:633540/1‑133 (MQ=255)                                                                                                                             gCATACAACTGGCGCAGGGCTATCTCGGACGCCCCGATCTGACCGCCAGCCGCTTTATTGCCGATCCTTTTGCCCCAGGTGAACGGATGTACCGTACCAGAGACGTTGCCCGCTGGCTGGATAACGGcgcg    <  2:447928/131‑1 (MQ=255)                                                                                                                             gCATACAACTGGCGCAGGGCTATCTCGGACGCCCCGATCTGACCGCCAGCCGCTTTATTGCCGATCCTTTTGCCCCAGGTGAACGGATGTACCGTACCAGAGACGTTGCCCGCTGGCTGGATAACGGcgcg    >  1:447928/1‑131 (MQ=255)                                                                                                                                 |                                                                                                                                 CTGGCACAGGTGCGCGGCAGCAGTGTGCCGATTGGTTATCCGGTATGGAATACGGGTCTGCGTATTCTTGATGCGATGATGCATCCGGTGCCGCCGGGTGTGGCGGGTGATCTCTATCTCACTGGCATTCAACTGGCGCAGGGCTATCTCGGACGCCCCGATCTGACCGCCAGCCGCTTTATTGCCGATCCTTTTGCCCCAGGTGAACGGATGTACCGTACCGGAGACGTTGCCCGCTGGCTGGATAACGGCGCGGT  >  NC_000913/616449‑616705

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑