Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	717,029	A→G	Y413H (TAC→CAC)	potE ←	putrescine:H(+) symporter/putrescine:ornithine antiporter PotE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	717,029	0	A	G	100.0%	156.7 / NA	43	Y413H (TAC→CAC)	potE	putrescine:H(+) symporter/putrescine:ornithine antiporter PotE
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (25/18);  total (25/18)

GATCATTCAATCGCCCTTTTTTACTTTGTTTTATTAACCGTGTTTATTTTTCAGTTCAAAGCGTGGTGATACCAGACCATACAGTGTCCAACCAAGGAAGGTCACGATGGAACCGTACAGCATGGCTTCTTCCCCGGATGAGTACAGCGCATAAAAGCTATACATCGCGCCAACAAAAGCAACAAAGTTTGCAACTTTCGCTTTTGAGGGGGGCACATTAGCGACCTTCTGAATAATGACTAATG  >  NC_000913/716913‑717157                                                                                                                     |                                                                                                                                 gATCATTCAATCGCCCTTTTTTACTTTGTTTTATTAACCGTGTTTATTTTTCAGTTCAAAGCGTGGTGATACCAGACCATACAGTGTCCAAACAAGGAAGTTCAGGGTGAAACCGTGAAGCATGGCTTCTTcc                                                                                                                  >  2:306815/1‑133 (MQ=255)  aTCATTCATTCGCCCTTTTTTACTTTGTTTTATTAACCGTGTTTATTTTTCAGTTCAAAGCGTGGTGATACCAGACCATACAGTGTCCAACCAAGGAAGGTCACGATGGAACCGTGCAGCATGGCTTCTTccc                                                                                                                 <  1:185500/133‑1 (MQ=255)  aTCATTCAATCGCCCTTTTTTACTTTGTTTTATTAACCGTGTTTATTTTTCAGTTCAAAGCGTGGTGATACCAGACCATACAGTGTCCAACCAAGGAAGGTCACGATGGAACCGTGCAGCATGGCTTCTTccc                                                                                                                 >  1:95671/1‑133 (MQ=255)            cGCCCTTTTTTACTTTGTTTTATTAACCGTGTTTATTTTTCAGTTCAAAGCGTGGTGATACCAGACCATACAGTGTCCAACCAAGGAAGGTCACGATGGAACCGTGCAGCATGGCTTCTTCCCCGGATGAGTa                                                                                                       <  2:2849/133‑1 (MQ=255)                    tttACTTTGTTTTATCAACCGTGTTTGTTTTTGAGTTCAAAGCGTGGTGATACCAGACCATACAGTGTCCAACCAAGGAAGGTCACGATGGAACCGTGCAGCATGGCTTCTTCCCCGGATGAGTACAGCGCAt                                                                                               <  2:276032/133‑1 (MQ=255)                      tACTTTGTTTTATTAACCGTGTTTATTTTTCAGTTCAAAGCGTGGTGATACCAGACCATACAGTGTCCAACCAAGGAAGGTCACGATGGAACCGTGCAGCATGGCTTCTTCCCCGGATGAGTACAGCGCATaa                                                                                             <  1:479444/133‑1 (MQ=255)                      tACTTTGTTTTATTAACCGTGTTTATTTTTCAGTTCAAAGCGTGGTGATACCAGACCATACAGTGTCCAACCAAGGAAGGTCACGATGGAACCGTGCAGCATGGCTTCTTCCCCGGATGAGTACAGCGCATaa                                                                                             <  2:158718/133‑1 (MQ=255)                      tACTTTGTTTTATTAACCGTGTTTATTTTTCAGTTCAAAGCGTGGTGATACCAGACCATACAGTGTCCAACCAAGGAAGGTCACGATGGAACCGTGCAGCATGGCTTCTTCCCCGGATGAGTACAGCGCATaa                                                                                             <  2:415495/133‑1 (MQ=255)                      tACTTTGTTTTATTAACCGTGTTTATTTTTCAGTTCAAAGCGTGGTGATACCAGACCATACAGTGTCCAACCAAGGAAGGTCACGATGGAACCGTGCAGCATGGCTTCTTCCCCGGATGAGTACAGCGCATaa                                                                                             <  1:384559/133‑1 (MQ=255)                        cTTTGTTTTATTAACCGTGTTTATTTTTCAGTTCAAAGCGTGGTGATACCAGACCATACAGTGTCCAACCAAGGAAGGTCACGATGGAACCGTGCAGCATGGCTTCTTCCCCGGATGAGTACAGCGCATaaaa                                                                                           <  2:95671/133‑1 (MQ=255)                           tGTTTTATTAACCGTGTTTATTTTTCAGTTCAAAGCGTGGTGATACCAGACCATACAGTGTCCAACCAAGGAAGGTCACGATGGAACCGTGAAGCATGGCATCTACCCAGGATGAGTACGGCGCATAAAAGCt                                                                                        >  2:197861/1‑133 (MQ=255)                             ttttATTAACCGTGTTTATTTTTCAGTTCAAAGCGTGGTGATACCAGACCATACAGTGTCCAACCAAGGAAGGTCACGATGGAACCGTGCAGCATGGCTTCTTCCCCGGATGAGTACAGCGCATAAAAGCtat                                                                                      >  2:104588/1‑133 (MQ=255)                                       cGTGTTTATTTTTCAGTTCAAAGCGTGGTGATACCAGACCATACAGTGTCCAACCAAGGAAGGTCACGATGGAACCGTGCAGCATGGCTTCTTCCCCGGATGAGTACAGCGCATAAAAGCTATACATCGCGcc                                                                            >  2:443734/1‑133 (MQ=255)                                           tttATTTTTCAGTTCAAAGCGTGGTGATACCAGACCATACAGTGTCCAACCAAGGAAGGTCACGATGGAACCGTGCAGCATGGCTTCTTCCCCGGATGAGTACAGCGCATAAAAGCTATACATCGCGCcaaca                                                                        >  1:319798/1‑133 (MQ=255)                                            ttATTTTTCAGTTCATAGCGTGGTGATACCGGACCATACAGTGTCCAACCAAGGAAGGTCACGATGGAACCGTGCAGCATGGCTTCTTCCCCGGATGAGTACAGCGCATAAAAGCTATACATCGCGCcaacaa                                                                       <  1:306815/133‑1 (MQ=255)                                                         cAAAGCGTGGTGATACCAGACCATACAGTGTCCAACCAAGGAAGGTCACGATGGAACCGTGCAGCATGGCTTCTTCCCCGGATGAGTACAGCGCATAAAAGCTATACATCGCGCCAACAAAAGCAACAAAGtt                                                          >  2:20287/1‑133 (MQ=255)                                                                 ggtgATACCAGACCATACAGTGTCCAACCAAGGAAGGTCACGATGGAACCGTGCAGCATGGCTTCTTCCCCGGATGAGTACAGCGCATAAAAGCTATACATCGCGCCAACAAAAGCAACAAAGTTTGCAACtt                                                  >  2:345419/1‑133 (MQ=255)                                                                 ggtgATACCAGACCATACAGTGTCCAACCAAGGAAGGTCACGATGGAACCGTGCAGCATGGCTTCTTCCCCGGATGAGTACAGCGCATAAAAGCTATACAACGCGCCAGCAAAAGCACCAAAGTTTGCAACtt                                                  >  2:232383/1‑133 (MQ=255)                                                                               aTACAGTGTCCAACCAAGGAAGGTCACGATGGAACCGTGCAGCATGGCTTCTTCCCCGGATGAGTACAGCGCATAAAAGCTATACATCGCGCCAACAAAAGCAACAAAGTTTGCAACTTTCGCTTTTGAgggg                                    >  2:32274/1‑133 (MQ=255)                                                                                 aCAGTGTCCAACCAAGGAAGGTCACGATGGAACCGTGCAGCATGGCTTCTTCCCCGGATGAGTACAGCGCATAAAAGCTATACATCGCGCCAACAAAAGCAACAAAGTTTGCAACTTTCGCTTTTGAgggggg                                  >  2:517506/1‑133 (MQ=255)                                                                                 aCAGTGTCCAACCAAGGAAGGTCACGATGGAACCGTGCAGCATGGCTTCTTCCCCGGATGAGTACAGCGCATAAAAGCTATACATCGCGCCAACAAAAGCAACAAAGTTTGCAACTTTCGCTTTTGAgggggg                                  >  1:96300/1‑133 (MQ=255)                                                                                  cAGTGTCCAACCAAGGAAGGTCACGATGGAACCGTGCAGCATGGCTTCTTCCCCGGATGAGTACAGCGCATAAAAGCTATACATCGCGCCAACAAAAGCAACAAAGTTTGCAACTTTCGCTTTTGAGGGGGGc                                 <  1:20287/133‑1 (MQ=255)                                                                                     tgtCCAACCAAGGAAGGTCACGATGGAACCGTGCAGCATGGCTTCTTCCCCGGATGAGTACAGCGCATAAAAGCTATACATCGCGCCAACAAAAGCAACAAAGTTTGCAACTTTCGCTTTTGAGGGGGGcaca                              >  1:624871/1‑133 (MQ=255)                                                                                      gtCCAACCAAGGAAGGTCACGATGGAACCGTGCAGCATGGCTTCTTCCCCGGATGAGTACAGCGCATAAAAGCTATACATCGCGCCAACAAAAGCAACAAAGTTTGCAACTTTCGCTTTTGAGGGGGGCACAt                             >  1:112510/1‑133 (MQ=255)                                                                                       tCCAACCAAGGAAGGTCACGATGGAACCGTGCAGCATGGCTTCTTCCCCGGATGAGTACAGCGCATAAAAGCTATACATCGCGCCAACAAAAGCAACAAAGTTTGCAACTTTCGCTTTTGAggg                                     <  2:147313/124‑1 (MQ=255)                                                                                       tCCAACCAAGGAAGGTCACGATGGAACCGTGCAGCATGGCTTCTTCCCCGGATGAGTACAGCGCATAAAAGCTATACATCGCGCCAACAAAAGCAACAAAGTTTGCAACTTTCGCTTTTGAggg                                     >  1:147313/1‑124 (MQ=255)                                                                                       tCCAACCAAGGAAGGTCACGATGGAACCGTGCAGCATGGCTTCTTCCCCGGATGAGTACAGCGCATAAAAGCTATACATCGCGCCAACAAAAGCAACAAAGTTTGCAACTTTCGCTTTTGAGGGGGGCACAtt                            >  1:143980/1‑133 (MQ=255)                                                                                       tCCAACCAAGGAAGGTCACGATGGAACCGTGCAGCATGGCTTCTTCCCCGGATGAGTACAGCGCATAAAAGCTATACATCGCGCCAACAAAAGCAACAAAGTTTGCAACTTTCGCTTTTGAGGGGGGCACAtt                            >  2:4386/1‑133 (MQ=255)                                                                                       tCCAACCAAGGAAGGTCACGATGGAACCGTGCAGCATGGCTTCTTCCCCGGATGAGTACAGCGCATAAAAGCTATACATCGCGCCAACAAAAGCAACAAAGTTTGCAACTTTCGCTTTTGAGGGGGGCACAtt                            >  2:456773/1‑133 (MQ=255)                                                                                       tCCAACCAAGGAAGGTCACGATGGAACCGTGCAGCATGGCTTCTTCCCCGGATGAGTACAGCGCATAAAAGCTATACATCGCGCCAACAAAAGCAACAAAGTTTGCAACTTTCGCTTTTGAGGGGGGCACAtt                            >  1:41642/1‑133 (MQ=255)                                                                                       tCCAACCAAGGAAGGTCACGATGGAACCGTGCAGCATGGCTTCTTCCCCGGATGAGTACAGCGCATAAAAGCTATACATCGCGCCAACAAAAGCAACAAAGTTTGCAACTTTCGCTTTTGAGGGGGGCACAtt                            >  1:316153/1‑133 (MQ=255)                                                                                        ccaaccaaGGAAGGTCACGATGGAACCGTGCAGCATGGCTTCTTCCCCGGATGAGTACAGCGCATAAAAGCTATACATCGCGCCAACAAAAGCAACAAAGTTTGCAACTTTCGCTTTTGAGGGGGGCACATTa                           >  1:596551/1‑133 (MQ=255)                                                                                        ccaaccaaGGAAGGTCACGATGGAACCGTGCAGCATGGCTTCTTCCCCGGATGAGTACAGCGCATAAAAGCTATACATCGCGCCAACAAAAGCAACAAAGTTTGCAACTTTCGCTTTTGAGGGGGGCACATTa                           >  2:420618/1‑133 (MQ=255)                                                                                        ccaaccaaGGAAGGTCACGATGGAACCGTGCAGCATGGCTTCTTCCCCGGATGAGTACAGCGCATAAAAGCTATACATCGCGCCAACAAAAGCAACAAAGTTTGCAACGTTCGCTTTAGAGGGAGGCACAtaa                           >  2:304160/1‑131 (MQ=255)                                                                                        ccaaccaaGGAAGGTCACGATGGAACCGTGCAGCATGGCTTCTTCCCCGGATGAGTACAGCGCATAAAAGCTATACATAGCGCCAACAAAAGCAACAAAGTTTGCAACTTTCGCTTTTGAGGGGGGCACATTa                           >  2:474648/1‑133 (MQ=255)                                                                                          aaccaaGGAAGGTCACGATGGAACCGTGCAGCATGGCTTCTTCCCCGGATGAGTACAGCGCATAAAAGCTATACATCGCGCCAACAAAAGCAACAAAGTTTGCAACTTTCGCTTTTGAGGGGGGCACATTAGc                         >  2:483296/1‑133 (MQ=255)                                                                                           accaaGGAGGGTCACTATGGAATCGTGCAGGATGGCTTCTTCCCCGGATGAGTACAGCGCATAAAAGCTATACATCGCGCCAACAAAAGCAACAAAGTTTGCAACTTTCGCTTTTGAGGGGGGCACATTAGCg                        <  1:232383/133‑1 (MQ=255)                                                                                           accaaGGAAGGTCACGATGGAACCGTGCAGCATGGCTTCTTCCCCGGATGAGTACAGCGCATAAAAGCTATACATCGCGCCAACAAAAGCAACAAAGTTTGCAACTTTCGCTTTTGAGGGGGGCACATTAGCg                        <  1:443734/133‑1 (MQ=255)                                                                                           accaaGGAAGGTCACGATGGAACCGTGCAGCATGGCTTCTTCCCCGGATGAGTACAGCGCATAAAAGCTATACATCGCGCCAACAAAAGCAACAAAGTTTGCAACTTTCGCTTTTGAGGGGGGCACATTAGCg                        <  1:4386/133‑1 (MQ=255)                                                                                                ggaaggTCACGATGGAACCGTGCAGCATGGCTTCTTCCCCGGATGAGTACAGCGCATAAAAGCTATACATCGCGCCAACAAAAGCAACAAAGTTTGCAACTTTCGCTTTTGAGGGGGGCACATTAGCGACCtt                   <  1:517506/133‑1 (MQ=255)                                                                                                             ggAACCGTGCAGCATGGCTTCTTCCCCGGATGAGTACAGCGCATAAAAGCTATACATCGCGCCAACAAAAGCAACAAAGTTTGCAACTTTCGCTTTTGAGGGGGGCACATTAGCGACCTTCTGAATAATGACt      <  1:319581/133‑1 (MQ=255)                                                                                                                 ccGTGCAGCATGGCTTCTTCCCCGGATGAGTACAGCGCATAAAAGCTATACATCGCGCCAACAAAAGCAAGAAAGTTTGCAACTTTCGCTTTTGAGGGGGGCACATTAGCGACCTTCTGAATAATGACTAATg  <  2:96300/133‑1 (MQ=255)                                                                                                                 ccGTGCAGCATGGCTTCTTCCCCGGATGAGTACAGCGCATAAAAGCTATACATCGCGCCAACAAAAGCAACAAAGTTTGCAACTTTCGCTTTTGAGGGGGGCACATTAGCGACCTTCTGAATAATGACTAATg  <  1:480242/133‑1 (MQ=255)                                                                                                                     |                                                                                                                                 GATCATTCAATCGCCCTTTTTTACTTTGTTTTATTAACCGTGTTTATTTTTCAGTTCAAAGCGTGGTGATACCAGACCATACAGTGTCCAACCAAGGAAGGTCACGATGGAACCGTACAGCATGGCTTCTTCCCCGGATGAGTACAGCGCATAAAAGCTATACATCGCGCCAACAAAAGCAACAAAGTTTGCAACTTTCGCTTTTGAGGGGGGCACATTAGCGACCTTCTGAATAATGACTAATG  >  NC_000913/716913‑717157

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑