Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	803,263	G→A	V459V (GTG→GTA)	ybhI →	putative tricarboxylate transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	803,263	0	G	A	100.0%	111.9 / NA	34	V459V (GTG→GTA)	ybhI	putative tricarboxylate transporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (13/21);  total (13/21)

TATGGCGGCATGGTTACTCACTATGGCGGCGCGGCAGGTCCGGTCATCTTTGGCGTGGGTTATAACGATATTAAATCCTGGTGGTTGGTCGGTGCGGTACTGACGATATTAACCTTCCTGGTGCATATCACCCTCGGCGTGTGGTGGTGGAATATGCTGATCGGCTGGAACATGCTGTAAATATTCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCACTTACTTATGTA  >  NC_000913/803141‑803390                                                                                                                           |                                                                                                                                tATGGCGGCATGGTTACTCACTATGGCGGCGCGGCAGGTCCGGTCATCTTTGGCGTGGGTTATAACGATATTAAATCCTGGTGGTTGGTCGGTGCGGTACTGACGATATTAACCTTCCTGGTACATATCAccc                                                                                                                       <  1:426678/133‑1 (MQ=255)  aTGGCGGCATGGTTACTCACTATGGCGGCGCGGCAGGTCCGGTCATCTTTGGCGTGGGTTATAACGATATTAAATCCTGGTGGTTGGTCGGTGCGGTACTGACGATATTAACCTTCCTGGTACATATCACCCt                                                                                                                      <  1:107240/133‑1 (MQ=255)        gcATGGTTACTCACTATGGCGGCGCGGCAGGTCCGGTCATCTTTGGCGTGGGTTATAACGATATTAAATCCTGGTGGTTGGTCGGTGCGGTCCTGACGATATTAACCTACCTGGTACGTATCTCCCTCGGCgt                                                                                                                >  2:275237/1‑133 (MQ=255)        gcATGGTTACTCACTATGGCGGCGCGGCAGGTCCGGTCATCTTTGGCGTGGGTTATAACGATATTAAATCCTGGTGGTTGGTCGGTGCGGTACTGACGATATTAACCTTCCTGGTACATATCACCCTCGGCgt                                                                                                                >  2:21621/1‑133 (MQ=255)         cATGGTTACTCACTATGGCGGCGCGGCAGGTCCGGTCATCTTTGGCGTGGGTTATAACGATATTAAATCCTGGTGGTTGGTCGGTGCGGTACTGACGATATTAACCTTCCTGGTACATATCACCCTCGGCgtg                                                                                                               <  1:30967/133‑1 (MQ=255)              ttACTCACTATGGCGGCGCGGCAGGTCCGGTCATCTTTGGCGTGGGTTATAACGATATTAAATCCTGGTGGTTGGTCGGTGCGGTACTGACGATATTAACCTTCCTGGTACATATCACCCTCGGCGTgtggtg                                                                                                          <  2:342329/133‑1 (MQ=255)              ttACTCACTATGGCGGCGCGGCAGGTCCGGTCATCTTTGGCGTGGGTTATAACGATATTAAATCCTGGTGGTTGGTCGGTGCGGTACTGACGATATTAACCTTCCTGGTACATATCACCCTCGGCGTgtggtg                                                                                                          <  1:28538/133‑1 (MQ=255)                   cACTATGGCGGCGCGGCAGGTCCGGTCATCTTTGGCGTGGGTTATAACGATATTAAATCCTGGTGGTTGGTCGGTGCGGTACTGACGATATTAACCTTCCTGGTACATATCACCCTCGGCGTGTGGTGGTGGa                                                                                                     <  2:114865/133‑1 (MQ=255)                             gcgcggcAGGTCCGGTCATCTTTGGCGTGGGTTATAACGATATTAAATCCTGGTGGTTGGTCGGTGCGGTACTGACGATATTAACCTTCCTGGTACATATCACCCTCGGCGTGTGGTGGTGGAATATGCTGAt                                                                                           <  2:385400/133‑1 (MQ=255)                             gcgcggcACGTCCGGCCACCTTTGGCGTGGCTTATAACGATATTAAATCCTGGTGGTTGGTCGGTGCGGTACTGACGATATTAACCTTCCTGGTACATATCACCCTCGGCGTGTGGTGGTGGAATATGCTGAt                                                                                           <  1:177837/133‑1 (MQ=255)                              cgcggcAGGTCCGGTCATCTTTGGCGTGGGTTATAACGATATTAAATCCTGGTGGTTGGTCGGTGCGGTACTGACGATATTAACCTTCCTGGTACATATCACCCTCGGCGTGTGGTGGGGGAATATGCTGATc                                                                                          >  2:147433/1‑133 (MQ=255)                                cggcAGGTCCGGTCATCTTTGGCGTGGGTTATAACGATATTAAATCCTGGTGGTTGGTCGGTGCGGTACTGACGATATTAACCTTCCTGGTACATATCACCCTCGGCGTGTGGTGGTGGAATATGCTGATCgg                                                                                        >  2:98999/1‑133 (MQ=255)                                     ggTCCGGTCATCTTTAGCTTGGATTATAACGATATTAAATCCTGGTGGTTGGTCGGTGCGGTACTGACGATATTAACCTTCCTGGTACATATCACCCTCGGCGTGTGGTGGTGGAATATGCTGATCGGCTGGa                                                                                   <  1:196158/133‑1 (MQ=255)                                           gTCATCTTTGGCGTGGGTTATAACGATATTAAATCCTGGTGGTTGGTCGGTGCGGTACTGACGATATTAACCTTCCTGGTACATATCACCCTCGGCGTGTGGTGGTGGAATATGCTGATCGGCTGGAACATGc                                                                             <  2:57404/133‑1 (MQ=255)                                           gTCATCTTTGCCGTGGGTTATAACCATAATAAATCCTGGTGCTTGGTCGGTGCGGTACTGACGATATTAACCTTCCTGGTACATATCACCCTCGGCGTGTGGTGGTGGAATATGCTGATCGGCTGGAACATGc                                                                             <  2:206353/133‑1 (MQ=255)                                                    ggCGTGGGTTATAACGATATTAAATCCTGGTGGTTGGTCGGTGCGGTACTGACGATATTAACCTTCCTGGTACATATCACCCTCGGCGTGTGGTGGTGGAATATGCTGATCGGCTGGAACATGCTGTAAatat                                                                    <  1:39216/133‑1 (MQ=255)                                                    ggCGTGGGATATAACGATCTTAAATCCTGGAGGTTGGTCGGTGCGGTACTGACGATATTAACCTTCCTGGTACATATCACCCTCGGCGTGTGGTGGTGGAATATGCTGATCGGCTGGAACATGCTGTAAatat                                                                    <  1:266317/133‑1 (MQ=255)                                                                  cGATATTAAATCCTGGTGGTTGGTCGGTGCGGTACTGACGATATTAACCTTCCTGGTACATATCACCCTCGGCGTGTGGTGGTGGAATATGCTGATCGGCTGGAACATGCTGTAAATATTCTCGTCATACTTc                                                      <  1:21621/133‑1 (MQ=255)                                                                     tatTAAATCCTGGTGGTTGGTCGGTGCGGTACTGACGATATTAACCTTCCTGGTACATATCACCCTCGGCGTGTGGTGGTGGAATATGCTGATCGGCTGGAACATGCTGTAAATATTCTCGTCATACTTCAAg                                                   >  2:94865/1‑133 (MQ=255)                                                                     tatTAAATCCTGGTGGTTGGTCGGTGCGGTACTGACGATATTAACCTTCCTGGTACATATCACCCTCGGCGTGTGGTGGTGGAATATGCTGATCGGCTGGAACATGCTGTAAATATTCTCGTCATACTTCAAg                                                   >  2:115757/1‑133 (MQ=255)                                                                      atTAAATCCTGGTGGTTGGTCGGTGCGGTACTGACGATATTAACCTTCCTGGTACATATCACCCTCGGCGTGTGGTGGTGGAATATGCTGATCGGCTGGAACATGCTGTAAATATTCTCGTCATACTTCAAGt                                                  <  1:115757/133‑1 (MQ=255)                                                                               tggtggTTGGTCGGTGCGGTACTGACGATATTAACCTTCCTGGTACATATCACCCTCGGCGTGTGGTGGTGGAATATGCTGATCGGCTGGAACATGCTGTAAATATTCTCGTCATACTTCAAGTTGCATGtgc                                         >  1:516527/1‑133 (MQ=255)                                                                                 gtggtTGGTCGGTGCGGTACTGACGATATTAACCTTCCTGGTACATATCACCCTCGGCGTGTGGTGGTGGAATATGCTGATCGGCTGGAACATGCTGTAAATATTCTCGTCATACTTCAAGTTGCGTGtgctg                                       >  2:612899/1‑133 (MQ=255)                                                                                 gtggtTGGTCGGTGCGGTACTGACGATATTAACCTTCCTGGTACATATCACCCTCGGCGTGTGGTGGTGGAATATGCTGATCGGCTGGAACATGCTGTAAATATTCTCGTCATACTTCAAGTTGCATGtgctg                                       >  1:457598/1‑133 (MQ=255)                                                                                          cGGTGCGGTACTGACGATATTAACCTTCCTGGTACATATCACCCTCGGCGTGTGGTGGTGGAATATGCTGATCGGCTGGAACATGCTGTAAATATTCTCGTCATACTTCAAGTTGCATGTGctgcgtctgcgt                              <  2:381589/133‑1 (MQ=255)                                                                                                  tACTGACGATATTAACCTTCCTGGTACATATCACCCTCGGCGTGTGGTGGTGGAATATGCTGATCGGCTGGAACATGCTGTAAATATTCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCAc                      >  1:105963/1‑133 (MQ=255)                                                                                                   aCTGACGACATTAACCTTACTGGTACATATCACCCTCGGCGTGTGGTGGTGGAATATGCTGATCGGCTGGAACATGCTGTAAATATTCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCAcc                     <  1:270572/133‑1 (MQ=255)                                                                                                       aCGATATTAACCTTCCTGGTACATATCACCCTCGGCGTGTGGTGGTGGAATATGCTGATCGGCTGGAACATGCTGTAAATATTCTCGTCATGCTTCAAGTCGCAGGTGCTGCGTCTGGGTTCGCTCACCCCAg                 >  2:270572/1‑133 (MQ=255)                                                                                                           tatTAACCTTCCTGGTACATATCACCCTCGGCGTGTGGTGGTGGAATATGCTGATCGGCTGGAACATGCTGTAAATATTCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCac             <  2:105963/133‑1 (MQ=255)                                                                                                            atTAACCTTCCTGGTACATATCACCCTCGGCGTGTGGTGGTGGAATATGCTGATCGGCTGGAACATGCTGTAAATATTCTCGTCATACTTCAAGTTGCATGTGCTGCGTCAGCGGTCGCTCACCACAGTCact            >  1:166925/1‑133 (MQ=255)                                                                                                               aaCCTTCCTGGTACATATCACCCTCGGCGTGTGGTGGTGGAATATGCTGATCGGCTGGAACATGCTGTAAATATTCTCGTCATACTTCAAGTTGCGTGTGCTGCGTCTGCGTTCGCTCACCCCAGTCacttac         <  1:612899/133‑1 (MQ=255)                                                                                                                 ccTTCCTGGTACATATCACCCTCGGCGTGTGGTGGTGGAATATGCTGATCGGCTGGAACATGCTGTAAATATTCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCacttactt       <  1:98999/133‑1 (MQ=255)                                                                                                                     ccTGGTACATATCACCCTCGGCGTGTGGTGGTGGAATATGCTGATCGGCTGGAACATGCTGTAAATATTCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCACTTACTTATGt   >  1:369622/1‑133 (MQ=255)                                                                                                                     ccTGGTACATATCACCCTCGGCGTGTGGTGGTGGAATATACTGATCGGCTGGAACATGCTGTAAATATTCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCACACGCCAGTCACTGACTTATGt   >  1:170505/1‑133 (MQ=25)                                                                                                                      cTGGTACATATCACCCTCGGCGTGTGGTGGTGGAATATGCTGATCGGCTGGAACATGCTGTAAATATTCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCACTTACTTATGTa  <  2:642836/133‑1 (MQ=255)                                                                                                                           |                                                                                                                                TATGGCGGCATGGTTACTCACTATGGCGGCGCGGCAGGTCCGGTCATCTTTGGCGTGGGTTATAACGATATTAAATCCTGGTGGTTGGTCGGTGCGGTACTGACGATATTAACCTTCCTGGTGCATATCACCCTCGGCGTGTGGTGGTGGAATATGCTGATCGGCTGGAACATGCTGTAAATATTCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCACTTACTTATGTA  >  NC_000913/803141‑803390

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑