Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	925,362	G→A	G700D (GGC→GAC)	clpA →	ATP‑dependent Clp protease ATP‑binding subunit ClpA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	925,362	0	G	A	100.0%	71.4 / NA	23	G700D (GGC→GAC)	clpA	ATP‑dependent Clp protease ATP‑binding subunit ClpA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (11/12);  total (11/12)

CCATCAGGTGGTGGATAAATTCATCGTCGAGTTGCAGGTTCAGCTGGATCAGAAAGGTGTTTCTCTGGAAGTGAGCCAGGAAGCGCGTAACTGGCTGGCCGAGAAAGGTTACGACCGGGCAATGGGCGCTCGTCCGATGGCGCGTGTCATCCAGGACAACCTGAAAAAACCGCTCGCCAACGAACTGCTGTTTGGTTCGCTGGTGGACGGCGGTCAGGTCACCGTCGCGCTGGATAAAGAGAAAAATGAGCT  >  NC_000913/925237‑925488                                                                                                                              |                                                                                                                               ccATCAGGTGGTGGATAAATTCATCGTCGAGTTGCAGGTTCAGCTGGATCAGAAAGGTGTTTCTCTGGAAGTGAGCCAGGAAGCGCGTAACTGGCTGGCCGAAAAAGGTTGCGAACGAGCAATGAACGCTCGt                                                                                                                         >  1:194368/1‑133 (MQ=255)        gtggtgGATAAATTCATCGTCGAGTTGCAGGTTCAGCTGGATCAGAAAGGTGTTTCTCTGGAAGTGAGCCAGGAAGCGCGTAACTGGCTGGCCGAGAAAGGTTACGACCGGGCAATGGACGCTCGTCCGATgg                                                                                                                  >  2:367407/1‑133 (MQ=255)          ggtggATAAATTCATCGTCGAGTTGCAGGTTCAGCTGGATCAGAAAGGTGTTTCTCTGGAAGTGAGCCAGGAAGCGCGTAACTGGCTGGCCGAGAAAGGTTACGACCGGGCAATGGACGCTCGTCCGATGgcg                                                                                                                >  1:86153/1‑133 (MQ=255)           gtggATAAATTCATCGTCGAGTTGCAGGTTCAGCTGGATCAGAAAGGTGTTTCTCTGGAAGTGAGCCAGGAAGCGCGTAACTGGCTGGCCGAGAAAGGTTACTACCGGGCAATGGACGCTCGTCGGATGgcgc                                                                                                               >  1:198082/1‑133 (MQ=255)              gATAAATTCATCGTCGAGTTGCAGGTTCAGCTGGATCAGAAAGGTGTTTCTCTGGAAGTGAGCCAGGAAGCGCGTAACTGGCTGGCCGAGAAAGGTTACGACCGGGCAATGGACGCTCGTCCGATGGCGCgtg                                                                                                            <  2:566615/133‑1 (MQ=255)              gATAAATTCATCGTCGAGTTGCAGGTTCAGCTGGATCAGAAAGGTGTTTCTCTGGAAGTGAGCCAGGAAGCGCGTAACTGGCTGGCCGAGAAAGGTTACGACCGGGCAATGGACGCTCGTCCGATGGCGCgtg                                                                                                            <  2:559466/133‑1 (MQ=255)              gATAAATTCATCGTCGAGTTGCAGGTTCAGCTGGATCAGAAAGGTGTTTCTCTGGAAGTGAGCCAGGAAGCGCGTAACTGGCTGGCCGAGAAAGGTTACGACCGGGCAATGGACGCTCGTCCGATGGCGCgtg                                                                                                            <  2:559076/133‑1 (MQ=255)              gATAAATTCATCGTCGAGTTGCAGGTTCAGCTGGATCAGAAAGGTGTTTCTCTGGAAGTGAGCCAGGAAGCGCGTAACTGGCTGGCCGAGAAAGGTTACGACCGGGCAATGGACGCTCGTCCGATGGCGCgtg                                                                                                            <  1:367407/133‑1 (MQ=255)              gATAAATTCATCGTCGAGTTGCAGGTTCAGCTGGATCAGAAAGGTGTTTCTCTGGAAGTGAGCCAGGAAGCGCGTAACTGGCTGGCCGAGAAAGGTTACGACCGGGCAATGGACGCTCGTCCGATGGCGCgtg                                                                                                            >  1:559076/1‑133 (MQ=255)              gATAAATTCATCGTCAAGTTGCAGCTTCTGCTCGATCAGAATGGTGTTTCTCTGGAAGTGAGCCAGGAAGCGCGTAACTGGCTGGCCGAGAAAGGTTACGACCGGGCAATGGACGCTCGTCCGATGGCGCgtg                                                                                                            <  2:194368/133‑1 (MQ=255)                   aTTCATCGTCGAGTTGCAGGTTCAGCTGGATCAGAAAGGTGTTTCTCTGGAAGTGAGCCAGGAAGCGCGTAACTGGCTGGCCGAGAAAGGTTACGACCGGACAATGGACGCTCGTCCTATGGTGCGTGTCATc                                                                                                       >  2:182102/1‑133 (MQ=255)                      cATCGTCGAGTTGCAGGTTCAGCTGGATCAGAAAGGTGTTTCTCTGGAAGTAAGCCAGGAAGCGCGTAACTGGCTGGCCGAGAAAGGTTACGACCGGGCAATGGACGCTCGTCCGATGGCGCGTGTCATCCAg                                                                                                    >  1:20172/1‑133 (MQ=255)                                                          tgtTTCTCTGGAAGTGAGCCAGGAAGCGCGTAACTGGCTGGCCGAGAAAGGTTACGACCGGGCAATGGACGCTCGTCCGATGGCGCGTGTCATCCAGGACAACCTGAAAAAACCGCTCGCCAACGAActgctg                                                                >  1:638219/1‑133 (MQ=255)                                                            tttCTCTGGAAGTGAGCCAGGAAGCGCGTAACTGGCTGGCCGAGAAAGGTTACGACCGGGCAATGGACGCTCGTCCGATGGCGCGTGTCATCCAGGACAACCTGAAAAAAGTGCTCGGCAACGAACTGCTGtt                                                              >  1:286155/1‑133 (MQ=255)                                                            tttCTCTGGAAGTGAGCCAGGAAGCGCGTAACTGGCTGGCCGAGAAAGGTTACGACCGGGCAATGGACGCTCGTCCGATGGCGCGTGTCATCCAGGACAACCGGAAAAAAGCGCTCGGCAACGAACTGCTGtt                                                              >  1:309911/1‑133 (MQ=255)                                                                                  aGCGCGTAACTGGCTGGCCGAGAAAGGTTACGACCGGGCAATGGACGCTCGTCCGATGGCGCGTGTCATCCAGGACAACCTGAAAAAACCGCTCGCCAACGAACTGCTGTTTGGTTCGCTGGTGGACGGCggt                                        <  1:599470/133‑1 (MQ=255)                                                                                  aGCGCGTAACTGGCTGGCCGAGAAAGGTTACGACCGGGCAATGGACGCTCGTCCGATGGCGCGTGTCATCCAGGACAACCTGAAAAAACCGCTCGCCAACGAACTGCTGTTTGGTTCGCTGGTGGACGGCggt                                        <  2:73106/133‑1 (MQ=255)                                                                                  aGCGCGTAACTGGCTGGCCGAGAAAGGTTACGACCGGGCAATGGACGCTCGTCCGATGGCGCGTGTCATCCAGGACAACCTGAAAAAACCGCTCGCCAACGAACTGCTGTTTGGTTCGCTGGTGGACGGCggt                                        <  2:86153/133‑1 (MQ=255)                                                                                            tggctggcCCCGAAAGGTTACGACCGGGCAATGGACGCTCGCCCGATGGCGCGTGTCATCCAGGACAACCTGAAAAAACTGCTCGCCAACGAACTGCTGTTTGGTTCGCTGGTGGACGGCGGTCAGGTCACCg                              <  2:286155/133‑1 (MQ=255)                                                                                            tcgctggcCTAGAAACGTTACGACAGCGTAATTGACGCTCGACCGATGGCGCGTGTCATCCAGGACAACCTGAAAAAACCGCTCGCCAACGAACTGCTGTTTGGTTCGCTGGTGGACGGCGGTCAGGTCACCg                              <  2:309911/131‑1 (MQ=255)                                                                                              gctggcCGAGAAAGGTTACGACCGGGCAATGGACGCTCGTCCGATGGCGCGTGTCATCCAGGACAACCTGAAAAAACCGCTCGCCAACGAACTGCTGTTTTGTTCGCTGGTGGACGGCGGTCAAGTCACCgac                            >  2:295662/1‑131 (MQ=255)                                                                                                tggcCGAGAAAGGTTACGACCGGGCAATGGACGCTCGTCCGATGGCGCGTGTCATCCAGGACAACCTGAAAAAACCGCTCGCCAACGAACTGCTGTTTGGTTCGCTGGTGGACGGCGGTCAGGTCACCGTcgc                          <  2:319092/133‑1 (MQ=255)                                                                                                                      ggCAATGGACGCTCGTCCGATGGCGCGTGTCATCCAGGACAACCTGAAAAAACCGCTCGCCAACGAACTGCTGTTTGGTTCGCTGGTGGACGGCGGTCAGGTCACCGTCGCGCTGGATAAAGAGAAAAATGAg    >  1:574492/1‑133 (MQ=255)                                                                                                                        cAATGGACGCTCGTCCGATGGCGCGTGTCATCCAGGACAACCTGAAAAAACCGCTCGCCAACGAACTGCTGTTTGGTTCGCTGGTGGACGGCGGTCAGGTCACCGTCGCGCTGGATAAAGAGAAAAATGAGCt  <  1:487616/133‑1 (MQ=255)                                                                                                                              |                                                                                                                               CCATCAGGTGGTGGATAAATTCATCGTCGAGTTGCAGGTTCAGCTGGATCAGAAAGGTGTTTCTCTGGAAGTGAGCCAGGAAGCGCGTAACTGGCTGGCCGAGAAAGGTTACGACCGGGCAATGGGCGCTCGTCCGATGGCGCGTGTCATCCAGGACAACCTGAAAAAACCGCTCGCCAACGAACTGCTGTTTGGTTCGCTGGTGGACGGCGGTCAGGTCACCGTCGCGCTGGATAAAGAGAAAAATGAGCT  >  NC_000913/925237‑925488

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑