Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	998,063	A→G	T66A (ACC→GCC)	elfA →	putative laminin‑binding fimbrial subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	998,063	0	A	G	100.0%	157.8 / NA	44	T66A (ACC→GCC)	elfA	putative laminin‑binding fimbrial subunit
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (22/22);  total (22/22)

TAATGCTATCACGGATGGCTCAGTAACATTTAATGGTAAAGTTATTGCTCCAGCTTGTACCCTGGTAGCTGCGACGAAAGATTCCGTGGTGACTTTGCCAGATGTTAGTGCCACGAAGTTGCAAACCAATGGTCAGGTTTCTGGCGTGCAAATTGATGTGCCAATTGAATTAAAAGATTGTGATACTACCGTAACAAAAAATGCAACGTTCACCTTTAATGGCACTGCGGATACTACTCAGATTAC  >  NC_000913/997939‑998184                                                                                                                             |                                                                                                                          tAATGCTATCACGGATGGCTCAGTAACATTTAATGGTAAAGTTATTGCTCCAGCTTGTACCCTGGTAGCTGCGACGAAAGATTCCGTGGTGACTTTGCCAGATGTTAGTGCCACGAAGTTGCAAGCCAATGGt                                                                                                                   <  2:595984/133‑1 (MQ=255) tAATGCTATCACGGATGGCTCAGTAACATTTAATGGTAAAGTTATTGCTCCAGCTTGTACCCTGGTAGCTGCGACGAAAGATTCCGTGGTGACTTTGCCAGATGTTAGTGCCACGAAGTTGCAAGCCAATGGt                                                                                                                   <  1:370598/133‑1 (MQ=255) tAATGCTATCACGGATGGCTCAGTAACATTTAATGGTAAAGTTATTGCTCCAGCTTGTACCCTGGTAGCTGCGACGAAAGATTCCGTGGTGACTTTGCCAGATGTTAGTGCCACGAAGTTGCAAGCCAATGGt                                                                                                                   <  2:360723/133‑1 (MQ=255)   aTGCTATCACGGATGGCTCAGTAACATTTAATGGTAAAGTTATTGCTCCAGCTTGTACCCTGGTAGCTGCGACGAAAGATTCCGTGGTGACTTTGCCAGATGTTAGTGCCACGAAGTTGCAAGCCAATGGTCa                                                                                                                 <  1:90686/133‑1 (MQ=255)       tATCACGGATGGCTCAGTAACATTTAATGGTAAAGTTATTGCTCCAGCTTGTACCCTGGTAGCTGCGACGAAAGATTCCGTGGTGACTTTGCCAGATGTTAGTGCCACGAAGTTGCAAGCCAATGGTCAGGtt                                                                                                             >  1:563092/1‑133 (MQ=255)          cACGGATGACTCAGTTACGTTTAATGGTAAGGTTATTGCTCCAGCTTGTACCCTGGTAGCTGCGACGAAAGATTCCGTGGTGACTTTGCCAGATGTTAGTGCCACGAAGTTGCAAGCCAATGGTCAGGTTTCt                                                                                                          <  1:265369/133‑1 (MQ=255)                    tCAGTAACATTTAATGGTAAAGTTATTGCTCCAGCTTGTACCCTGGTAGCTGCGACGAAAGATTCCGTGGTGACTTTGCCAGATGTTAGTGCCACGAAGTTGCAAGCCAATGGTCAGGTTTCTGGCGTGCaaa                                                                                                <  1:602804/133‑1 (MQ=255)                             tttAATGGTAAAGTTATTGCTCCAGCTTGTACCCTGGTAGCTGCGACGAAAGATTCCGTGGTGACTTTGCCAGATGTTAGTGCCACGAAGTTGCAAGCCAATGGTCAGGTTTCTGGCGTGCAAATTGATGTGc                                                                                       >  1:563046/1‑133 (MQ=255)                                aaTGGTAAAGTTATTGCTCCAGCTTGTACCCTGGTAGCTGCGACGAAAGATTCCGTGGTGACTTTGCCAGATGTTAGTGCCACGAAGTTGCTAGCCAATGATCAGGCTGTTGGCGTGGAAATTGATGTGCCaa                                                                                    >  2:297001/1‑133 (MQ=255)                                 aTGGTAAAGTTATTGCTCCAGCTTGTACCCTGGTAGCTGCGACGAAAGATTCCGTGGTGACTTTGCCAGATGTTAGTGCCACGAAGTTGCAAGCCAATGGTCAGGTTTCTGGCGTGCAAATTGATGTGCCAAt                                                                                   <  2:563046/133‑1 (MQ=255)                                  tGGTAAAGTTATTGCTCCAGCTTGTACCCTGGTAGCTGCGACGAAAGATTCCGTGGTGACTTTGCCAGATGTTAGTGCCACGAAGTTGCAAGCCAATGGTCAGGTTTCTGGCGTGCAAATTGATGTGCCAAtt                                                                                  >  1:380672/1‑133 (MQ=255)                                  tGGTAAAGTTATTGCTCCAGCTTGTACCCTGGTAGCTGCGACGAAAGATTCCGTGGTGACTTTGCCAGATGTTAGTGCCACGAAGTTGCAAGCCAATGGTCAGGTTTCTGGCGTGCAAATTGATGTGCCAAtt                                                                                  >  1:153374/1‑133 (MQ=255)                                      aaaGTTATTGCTCCAGCTTGTACCCTGGTAGCTGCGACGAAAGATTCCGTGGTGACTTTGCCAGATGTTAGTGCCACGAAGTTGCAAGCCAATGGTCAGGTTTCTGGCGTGCAAATTGATGTGCCAATTGAAt                                                                              >  1:94702/1‑133 (MQ=255)                                                    aGCTTGTACCCTGGTAGCTGCGACGAAAGATTCCGTGGTGACTTTGCCAGATGTTAGTGCCACGAAGTTGCAAGCCAATGGTCAGGTTTCTGGCGTGCAAATTGATGTGCCAATTGAATTAAAAGATTGTGAt                                                                >  2:18510/1‑133 (MQ=255)                                                          tACCCTGGTAGCTGCGACGAAAGATTCCGTGGTGACTTTGCCAGATGTTAGTGCCACGAAGTTGCAAGCCAATGGTCAGGTTTCTGGCGTGCAAATTGATGTGCCAATTGAATTAAAAGATTGTGATACTAcc                                                          >  1:403837/1‑133 (MQ=255)                                                          tACCCTGGTAGCTGCGACGAAAGATTCCGTGGTGACTTTGCCAGATGTTAGTGCCACGAAGTTGCAAGCCAATGGTCAGGTTTCTGGCGTGCAAATTGATGTGCCAATTGAATTAAAAGATTGTGATACTAcc                                                          >  1:461117/1‑133 (MQ=255)                                                           aCCCTGGTAGCTGCGACGAAAGATTCCGTGGTGACTTTGCCAGATGTTAGTGCCACGAAGTTGCAAGCCAATGGTCAGGTTTCTGGCGTGCAAATTGATGTGCCAATTGAATTAAAAGATTGTGATACTACCg                                                         >  1:322081/1‑133 (MQ=255)                                                                ggTAGCTGCGACGAAAGATTCCGTGGTGACTTTGCCAGATGTTAGTGCCACGAAGTTGCAAGCCAATGGTCAGGTTTCTGGCGTGCAAATTGATGTGCCAATTGAATTAAAAGATTGTGAtac                                                              >  2:35174/1‑123 (MQ=255)                                                                ggTAGCTGCGACGAAAGATTCCGTGGTGACTTTGCCAGATGTTAGTGCCACGAAGTTGCAAGCCAATGGTCAGGTTTCTGGCGTGCAAATTGATGTGCCAATTGAATTAAAAGATTGTGAtac                                                              <  1:35174/123‑1 (MQ=255)                                                                 gTAGCTGCGACGAAAGATTCCGTGGTGACTTTGCCAGATGTTAGTGCCACGAAGTTGCAAGCCAATGGTCAGGTTTCTGGCGTGCAAATTGATGTGCCAATTGAATTAAAAGATTGTGATACTACCGTAACaa                                                   >  1:150084/1‑133 (MQ=255)                                                                      tGCGACGAAAGATTCCGTGGTGACTTTGCCAGATGTTAGTGCCACGAAGTTGCAAGCCAATGGTCAGGTTTCTGGCGTGCAAATTGATGTGCCAATTGAATTAAAAGATTGTGATACTACCGTAACAAAAAAt                                              >  2:59879/1‑133 (MQ=255)                                                                       gCGACGAAAGATTCCGTGGTGACTTTGCCAGATGTTAGTGCCACGAAGTTGCAAGCCAATGGTCAGGTTTCTGGCGTGCAAATTGATGTGCCAATTGAATTAAAAGATTGTGATACTACCGTAACAAAAAATg                                             >  2:334553/1‑133 (MQ=255)                                                                          acgaAAGATTCCGTGGTGAATTTGCCAGATGTTAGTGCCACGAAGTTGCAAGCCAATGGTCAGGTTTCTGGCGTGCAAATTGATGTGCCAATTGAATTAAAAGATTGTGATACTACCGTAACAAAAAATGCaa                                          >  2:21811/1‑133 (MQ=255)                                                                             aaaGATTCCGTGGTGACTTTGCCAGATGTTAGTGCCACGAAGTTGCAAGCCAATGGTCAGGTTTCTGGCGTGCAAATTGATGTGCCAATTGAATTAAAAGATTGTGATACTACCGTAACAAAAAATGCAACGt                                       <  2:94702/133‑1 (MQ=255)                                                                             aaaGATTCAGTGGTGACTATGCTAGATGTTAGTGCCACGAAGTTGCAAGCCAATGGTCAGGTTTCTGGCGTGCAAATTGATGTGCCAATTGAATTAAAAGATTGTGATACTACCGTAACAAAAAATGCAACGt                                       <  1:297001/133‑1 (MQ=255)                                                                                  ttCCGTGGTGGCTTTACCAGATGTGAGTGCCACGAAGTTGCGAGCCAATGGTCAGGTTTCTGGCGTGCAAATTGATGTGCCAATTGAATTAAAAGATTGTGATACTACCGTAACAAAAAATGCAACGTTCAcc                                  <  2:235511/133‑1 (MQ=255)                                                                                          tgACTTTGCCAGATGTTAGTGCCACGAAGTTGCAAGCCAATGGTCAGGTTTCTGGCGTGCAAATTGATGTGCCAATTGAATTAAAAGATTGTGATACTACCGTAACAAAAAATGCAACGTTCACCTTTAATgg                          <  2:563092/133‑1 (MQ=255)                                                                                           gACTTTGCCAGATGTTAGTGCCACGAAGTTGCAAGCCAATGGTCAGGTTTCTGGCGTGCAAATTGATGTGCCAATTGAATTAAAAGATTGTGATACTACCGTAACAAAAAATGCAACGTTCACCTTTAATGGc                         <  2:375436/133‑1 (MQ=255)                                                                                            aCTTTGCCCGATGTTTGTCCCACGAAGTTGGAAGCCAATGGTCAGGTTTCTGGCGTGCAAATTGATGTGCCAATTGAATTAAAAGATTGTGATACTACCGTAACAAAAAATGCAACGTTCACCTTTAATGGCa                        <  1:205950/133‑1 (MQ=255)                                                                                            aCTTTGCCAGATGTTAGTGCCACGAAGTTGCAAGCCAATGGTCAGGTTTCTGGCGTGCAAATTGATGTGCCAATTGAATTAAAAGATTGTGATACTACCGTAACAAAAAATGCAACGTTCACCTTTAATGGCa                        <  2:461117/133‑1 (MQ=255)                                                                                              tttGCCAGCTGTTAGTGCAACGAAGTTGCACGCCAATGGTCAGGTTTCTGGCGTGCAAATTGATGTGCCAATTGAATTAAAAGATTGTGATACTACCGTAACAAAAAATGCAACGTTCACCTTTAATGGCACt                      <  1:209957/133‑1 (MQ=255)                                                                                              tttGCCAGATGTTAGTGCCACGAAGTTGCAAGCCAATGGTCAGGTTTCTGGCGTGCAAATTGATGTGCCAATTGAATTAAAAGATTGTGATACTACCGTAACAAAAAATGCAACGTTCACCTTTAATGGCACt                      <  1:59879/133‑1 (MQ=255)                                                                                                tGCCAGATGTTAGTGCCACGAAGTTGCAAGCCAATGGTCAGGTTTCTGGCGTGCAAATTGATGTGCCAATTGAATTAAAAGATTGTGATACTACCGTAACAAAAAATGCAACGTTCACCTTTAATGGCACTGc                    <  2:119258/133‑1 (MQ=255)                                                                                                   cAGATGTTAGTGCCACGAAGTTGCAAGCCAATGGTCAGGTTTCTGGCGTGCAAATTGATGTGCCAATTGAATTAAAAGATTGTGATACTACCGTAACAAAAAATGCAACGTTCACCTTTAATGGCACTGCGGa                 <  2:403837/133‑1 (MQ=255)                                                                                                   cAGATGTTAGGGCCACGAAGTTGCCAGCTAATCGTCAGGTTACTGGCGTGCAAATTGATGTGCCAATTGAATTAAAAGATTGTGATACTACCGTAACAAAAAATGCAACGTTCACCTTTAATGGCACTGCGGa                 <  2:234358/133‑1 (MQ=255)                                                                                                           aGTGCCACGAAGTTGCAAGCCAATGGTCAGGTTTCTGGCGTGCAAATTGATGTGCCAATTGAATTAAAAGATTGTGATACTACCGTCACAAAAAATGCAACGTTCACCTTTAATGGCACTGCGGATACTACTc         <  2:153374/133‑1 (MQ=255)                                                                                                           aGTGCCACGAAGTTGCAAGCCAATGGTCAGGTTTCTGGCGTGCAAATTGATGTGCCAATTGAATTAAAAGATTGTGATACTACCGTAACAAAAAATGCAACGTTCACCTTTAATGGCACTGCGGATACTACTc         <  1:21811/133‑1 (MQ=255)                                                                                                                cACGAAGTTGCAAGCCAATGGTCAGGTTTCTGGCGTGCAAATTGATGTGCCAATTGAATTAAAAGATTGTGATACTACCGTAACAAAAAATGCAACGTTCACCTTTAATGGCACTGCGGATACTACTCAGAtt    >  2:559098/1‑133 (MQ=255)                                                                                                                cACGAAGTTGCAAGCCAATGGTCAGGTTTCTGGCGTGCAAATTGATGTGCCAATTGAATTAAAAGATTGTGATACTACCGTAACAAAAAATGCAACGTTCACCTTTAATGGCACTGCGGATACTACTCAGAtt    >  1:336765/1‑133 (MQ=255)                                                                                                                 aCGAAGTTGCAAGCCAATGGTCAGGTTTCTGGCGTGCAAATTGATGTGCCAATTGAATTAAAAGATTGTGATACTACCGTAACAAAAAATGCAACGTTCACCTTTAATGGCACTGCGGATACTACTCAGATTa   >  2:567003/1‑133 (MQ=255)                                                                                                                 aCGAAGTTGCAAGCCAATGGTCAGGTTTCTGGCGTGCAAATTGATGTGCCAATTGAATTAAAAGATTGTGATACTACCGTAACAAAAAATGCAACGTTCACCTTTAATGGCACTGCGGATACTACTCAGATTa   >  1:11929/1‑133 (MQ=255)                                                                                                                  cGAAGTTGCAAGCCAATGGTCAGGTTTCTGGCGTGCAAATTGATGTGCCAATTGAATTAAAAGATTGTGATACTACCGTAACAAAAAATGCAACGTTCACCTTTAATGGCACTGCGGATACTACTCAGATTAc  >  2:385992/1‑133 (MQ=255)                                                                                                                  cGAAGTTGCAAGCCAATGGTCAGGTTTCTGGCGTGCAAATTGATGTGCCAATTGAATTAAAAGATTGTGATACTACCGTAACAAAAAATGCAACGTTCACCTTTAATGGCACTGCGGATACTACTCAGATTAc  >  1:333779/1‑133 (MQ=255)                                                                                                                  cGAAGTTGCAAGCCAATGGTCAGGTTTCTGGCGTGCAAATTGATGTGCCAATTGAATTAAAAGATTGTGATACTACCGTAACAAAAAATGCAACGTTCACCTATAATGGCACTGTGGATACTACGCAGATTAc  >  1:166388/1‑133 (MQ=255)                                                                                                                             |                                                                                                                          TAATGCTATCACGGATGGCTCAGTAACATTTAATGGTAAAGTTATTGCTCCAGCTTGTACCCTGGTAGCTGCGACGAAAGATTCCGTGGTGACTTTGCCAGATGTTAGTGCCACGAAGTTGCAAACCAATGGTCAGGTTTCTGGCGTGCAAATTGATGTGCCAATTGAATTAAAAGATTGTGATACTACCGTAACAAAAAATGCAACGTTCACCTTTAATGGCACTGCGGATACTACTCAGATTAC  >  NC_000913/997939‑998184

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑