| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,072,185 | C→T | A227T (GCT→ACT) | rutB ← | peroxyureidoacrylate/ureidoacrylate amidohydrolase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,072,185 | 0 | C | T | 95.8% | 71.8 / NA | 24 | A227T (GCT→ACT) | rutB | peroxyureidoacrylate/ureidoacrylate amidohydrolase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (10/13); minor base G (1/0); total (11/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.58e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTAAACTCCTTAAGCGATATGAGCAAAGGACGTGGGAGAAAGCGCGTCGCAGAATGTTTCGACGTCGCTGACCCAGCCAAAAAAGGTTTCGATATTGAACAACGCGGCTTTCTGCGCAAATTTCGGCCCCGCCTGGTGAGTTGCG > NC_000913/1072074‑1072306 | tACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTAAACTCCTTAAGCGATATGAGTAAAGGACGTGGGAGAAAgcgc < 1:546900/133‑1 (MQ=255) caccacGCCATCAGCCAGAGTGTCGGGAACCAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTAAACTCCTTAAGCGATATGAGTAAAGGACGTGGGAGAAAGCGCGt < 1:249371/133‑1 (MQ=255) cGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTAAACTCCTTAAGCGATATGAGTAAAGGGCGTGGGGGAAAGCGCGTCGCAg > 1:314414/1‑133 (MQ=255) cGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTAAACTCCTTAAGCGATATGAGTAAAGGACGTGGGAGAAAGCGCGTCGCAg > 2:514538/1‑133 (MQ=255) cGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTAAACTCCTTAAGCGATATGAGTAAAGGACGTGGGAGAAAGCGCGTCGCAg > 1:521441/1‑133 (MQ=255) cGCCATCAGCCAGCGTACCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTAAACTCCTTAAGCGATATGAGTAAAGGACGTGGGAGAAAGCGCGTCGCa < 2:487303/132‑1 (MQ=255) cGCCATCAGCCAGCGTACCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTAAACTCCTTAAGCGATATGAGTAAAGGACGTGGGAGAAAGCGCGTCGCa > 1:487303/1‑132 (MQ=255) ccATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGTATAATTACGGATTTTGGCATCGTTAAACTCCTTAAGCGATATGAGTAAAGGACGTGGGAGAAAGCGCGTCGCAGaa > 1:558000/1‑133 (MQ=255) ccATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTAAACTCCTTAAGCGATATGAGTAAAGGACGTGGGAGAAAGCGCGTCGCAGaa > 2:77824/1‑133 (MQ=255) aaCGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTAAACTCCTTAAGCGATATGAGTAAAGGACGTGGGAGAAAGCGCGTCGCAGAATGTTTCGACGTCGCTGAccc > 2:3975/1‑133 (MQ=255) cGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTAAACTCCTTAAGCGATATGAGTAAAGGACGTGGGAGAAAGCGCGTCGCAGAATGTTTCGACGTCGCTGACCCAGCCAAAAAAGGttt < 1:516146/133‑1 (MQ=255) cGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTAAACTCCTTAAGCGATATGAGTAAAGGACGTGGGAGAAAGCGCGTCGCAGAATGTTTCGACGTCGCTGACCCAGCCAAAAAAGGttt < 1:514538/133‑1 (MQ=255) cGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTAAACTCCTTAAGCGATATGAGTAAAGGACGTGGGAGAAAGCGCGTCGCAGAATGTTTCGACGTCGCTGACCCAGCCAAAAAAGGttt < 2:22131/133‑1 (MQ=255) cGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTAAACTCCTTAAGCGATATGAGTAAAGGACGTGGGAGAAAGCGCGTCGCAGAATGTTTCGACGTCGCTGACCCAGCCAAAAAAGGttt < 1:434502/133‑1 (MQ=255) cGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTAAACTCCTTAAGCGATATGAGTAAAGGACGTGGGAGAAAGCGCGTCGCAGAATGTTTCGACGTCGCTGACCCAGCCAAAAAAGGttt < 1:3975/133‑1 (MQ=255) tACGGATTTTGGCATCGTTAAACTCCTTAAGCGATATTAGGAAAAGACGTGGGAGAAAGCGCGTCGCAGAATGTTTCGGCGGCGCTGACCCAGCCAAAAAAGGTTTCGATATTGAACAACCCGGCTTTCTgcg > 2:43623/1‑133 (MQ=255) ggATTTTGGCATCGTTAAACTCCTTAAGCGATATGAGTAAAGGACGTGGGAGAAAGCGCGTCGCAGAATGTTTCGACGTCGCTGACCCAGCCAAAAAAGGTTTCGATATTGAACAACGCGGCTTTCTGCGCaa > 1:501174/1‑133 (MQ=255) ttGGCATCGTTAAACTCCTTAAGCGATATGAGTAAAGGACGTGGGAGAAAGCGCGTCGCAGAATGTTTCGACGTCGCTGACCCAGCCAAAAAAGGTTTCGATATTGAACAACGCGGCTTTCTGCGCAAATTTc > 1:89346/1‑133 (MQ=255) gCATCGTTAAGCTCCGTAAGCGACATAATTAAAGGACGTGGAAGAAAGCGCGTCGCAGAATGTTTCGACGTCGCTGACCCAGCCAAAAAAGGTTTCGATATTGAACAACGCGGCTTTCTGCGCAAATTTCGGc < 2:314414/133‑1 (MQ=255) gCATCGTTAAACTCCTTAAGCGATATGAGTAAAGGACGTGGGAGAAAGCGCGTCGCAGAATGTTTCGACGTCGCTGACCCAGCCAAAAAAGGTTTCGATATTGAACAACGCGGCTTTCTGCGCAAATTTCGGc < 1:77824/133‑1 (MQ=255) gTTAAACTCCTTAAGCGATATGAGTAAAGGACGTGGGAGAAAGCGCGTCGCAGAATGTTTCGACGTCGCTGACCCAGCCAAAAAAGGTTTCGATATTGAGCAACGCGGCTTTCTGCGCAAATTTCGGCCCCGc < 2:521441/133‑1 (MQ=255) tAAGCGATATGAGTAAAGGACGTGGGAGAAAGCGCGTCGCAGAATGTTTCGACGTCGCTGACCCAGCCAAAAAAGGTTTCGATATTGAACAACGCGGCTTACTGCGCAAATTTCGGCCCCGCCTGGTGAGTTg > 2:243033/1‑133 (MQ=255) aGCGATATGAGTAAAGGACGTGGGAGAAAGCGCGTCGCAGAATGTTTCGACGTCGCTGACCCAGCCAAAAAAGGTTTCGATATTGAACAACGCGGCTTTCTGCGCAAATTTCGGCCCCGCCTGGTGAGTTGCg < 2:501174/133‑1 (MQ=255) aGCGATATGAGTAAAGGACGTGGGAGAAAGCGCGTCGCAGAATGTTTCGACGTCGCTGACCCAGCCAAAAAAGGTTTCGATATTGAACAACGCGGCTTTCTGCGCAAATTTCGGCCCCGCCTGGTGAGTTGCg < 1:424983/133‑1 (MQ=255) | TACACCACGCCATCAGCCAGCGTGCCGGGAACGAAGGGGGCCAGCGGTGCGCTGCTGCCAGCGGGAATAATTACGGATTTTGGCATCGTTAAACTCCTTAAGCGATATGAGCAAAGGACGTGGGAGAAAGCGCGTCGCAGAATGTTTCGACGTCGCTGACCCAGCCAAAAAAGGTTTCGATATTGAACAACGCGGCTTTCTGCGCAAATTTCGGCCCCGCCTGGTGAGTTGCG > NC_000913/1072074‑1072306 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |