Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	206,738	T→C	V538A (GTC→GCC)	dnaE →	DNA polymerase III subunit alpha

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	206,738	0	T	C	100.0%	75.4 / NA	22	V538A (GTC→GCC)	dnaE	DNA polymerase III subunit alpha
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (12/10);  total (12/10)

CGCGCAAACTGGAAGGGGTCACCCGTAACGCCGGTAAGCACGCCGGTGGGGTGGTTATCGCGCCGACCAAAATTACCGATTTTGCGCCGCTTTACTGCGATGAAGAGGGCAAACATCCGGTCACCCAGTTTGATAAAAGCGACGTTGAATACGCCGGACTGGTGAAGTTCGACTTCCTTGGTTTGCGTACGCTCACCATCATCAACTGGGCGCTGGAGATGATCAACAAGCGGCGGGCGAAGAAT  >  NC_000913/206618‑206862                                                                                                                         |                                                                                                                             cgcgcAAACTGGAAGGGGTCACCCGTAACGCCGGTAAGCACGCCGGTGGGGTGGTTATCGCGCCGACCAAAATTACCGATTTTGCGCCGCTTTACTGCGATGAAGAGGGCAAACATCCGGCCACCCAGTTTGa                                                                                                                  >  2:549133/1‑133 (MQ=255) cgcgcAAACTGGAAGGGGTCACCCGTAACGCCGGTAAGCACGCCGGTGGGGTGGTTATCGCGCCGACCAAAATTACCGATTTTGCGCCGCTATACTGCGATGAACAGGCCAAACATCTGGCCACCCAGTTTGa                                                                                                                  >  2:222851/1‑133 (MQ=255)                         gTAACGCCGGTAAGCACGCCGGTGGGGTGGTTATCGCGCCGACCAAAA‑TACCGATTTTGCGCCGCTTTACTGCGATGAAGAGGGCAAACATCCGGCCACCCAGTTTGATAAAAGCGACGTTGAATACGCCGGa                                                                                         <  2:30512/133‑1 (MQ=255)                           aaCGCCGGTAAGCACGCCGGTGGGGTGGTTATCGCGCCGACCAAAATTACCGATTTTGCGCCGCTTTACTGCGATGAAGAGGGCAAACATCCGGCCACCCAGTTTGATAAAAGTGACGTTGAATACGCCGGAc                                                                                        >  2:400368/1‑133 (MQ=255)                                                      gtTATCGCGCCGACCAAAATTACCGATTTTGCGCCGCTTTACTGCGATGAAGAGGGCAAACATCCGGCCACCCAGTTTGATAAAAGCGACGTTGAATACGCCGGACTGGTGAAGTTCGACTTCCTTGGTTTGc                                                             >  2:159615/1‑133 (MQ=255)                                                       ttATCGCGTCGACCATAACTACCGATTTTGTGCCACTTTACTGCGATGAAGAGGGCAAACATCCGGCCACCCAGTTTGATAAAAGCGACGTTGAATACGCCGGACTGGTGAAGTTCGACTTCCTTGGTTTGCg                                                            <  1:304000/133‑1 (MQ=255)                                                       ttATCGCGCCGACCAAAATTACCGATTTTGCGCCGCTTTACTGCGATGAAGAGGGCAAACATCCGGCCACCCAGTTTGATAAAAGCGACGTTGAATACGCCGGACTGGTGAAGTTCGACTTCCTTGGTTTGCg                                                            >  2:511096/1‑133 (MQ=255)                                                            gcgcCGACCAAAATTACCGATTTTGCGCCGCTTTACTGCGATGAAGAGGGCAAACATCCGGCCACCCAGTTTGATAAAAGCGACGTTGAATACGCCGGACTGGTGAAGTTCGACTTCCTTGGTTTGCGTACGc                                                       <  1:549133/133‑1 (MQ=255)                                                              gcCGACCAAAATTACCGATTTTGCGCCGCTTTACTGCGATGAAGAGGGCAAACATCCGGCCACCCAGTTTGATAAAAGCGACGTTGAATACGCCGGACTGGTGAAGTTCGACTTCCTTGGTTTGCGTACGCTc                                                     >  2:627105/1‑133 (MQ=255)                                                               ccGACCAAAATTACCGATTTTGTGCCGCTTGACTGCGATGAAGAGGGCAAACATCCGGCCACCCAGTTTGATAAAAGCGACGTTGAATACGCCGGACTGGTGAAGTTCGACTTCCTTGGTTTGCGTACGCTCa                                                    <  1:222851/133‑1 (MQ=255)                                                               ccGACCAAAATTACCGATTTTGCGCCGCTTTACTGCGATGAAGAGGGCAAACATCCGGCCACCCAGTTTGATAAAAGCGACGTTGAATACGCCGGACTGGTGAAGTTCGACTTCCTTGGTTTGCGTACGCTCa                                                    <  1:131861/133‑1 (MQ=255)                                                               ccGACCAAAATTACCGATTTTGCGCCGCTTTACTGCGATGAAGAGGGCAAACATCCGGCCACCCAGTTTGATAAAAGCGACGTTGAATACGCCGGACTGGTGAAGTTCGACTTCCTTGGTTTGCGTACGCTCa                                                    <  2:17519/133‑1 (MQ=255)                                                                cGACCAAAATTACCGATTTTGCGCCGCTTTACTGCGATGAAGAGGGCAAACATCCGGCCACCCAGTTTGATAAAAGCGACGTTGAATACGCTGGACTGGTGAAGTTCGACTTCCTTGGTTTGCGTACGCTCAc                                                   >  1:8132/1‑133 (MQ=255)                                                                cGACCAAAATTACCGATTTTGCGCCGCTTTACTGCGATGAAGAGGGCAAACATCCGGCCACCCAGTTTGATAAAAGCGACGTTGAATACGCTGGACTGGTGAAGTTCGACTTCCTTGGTTTGCGTACGCTCAc                                                   <  2:8132/133‑1 (MQ=255)                                                                 gACCAAAAGTACCGAGTTGGCGACGCTTTAGTGCGATGAAGAGGGCAAACATCCGGCCACCCAGTTTGATAAAAGCGACGTTGAATACGCCGGACTGGTGAAGTTCGACTTCCTTGGTTTGCGTACGCTCAcc                                                  <  1:290834/133‑1 (MQ=255)                                                                                      gccgcTTTACTGCGATGAAGAGGGCAAACATCCGGCCACCCAGTTTGATAAAAGCGACGTTGAATACGCCGGACTGGTGAAGTTCGACTTCCTTGGTTTGCGTACGCTCACCATCATCAACTGGGCGCTGgag                             >  1:559365/1‑133 (MQ=255)                                                                                          cTTTACTGCGATGAAGAGGGCAAACATCCGGCCACCCAGTTTGATAAAAGCGACGTTGAATACGCCGGACTGGTGTAGTTCGACTTCCTTGGTTTGCGTACGCTCACCATCATCAACTGGGCGCTGGAgatga                         >  1:567502/1‑133 (MQ=255)                                                                                                    aTGAAGAGGGCAAACATCCGGCCACCCAGTTTGATAAAAGCGACGTTGAATACGCCGGACTGGTGAAGTTCGACTTCCTTGGTTTGCGTACGCTCACCATCATCAACTGGGCGCTGGAGATGATCAACAAgcg               >  2:44671/1‑133 (MQ=255)                                                                                                     tGAAGAGGGCAAACATCCGGCCACCCAGTTTGATAAAAGCGACGTTGAATACGCCGGACTGGTGAAGTTCGACTTCCTTGGTTTGCGTACGCTCACCATCATCAACTGGGCGCTGGAGATGATCAACAAgcgg              <  2:110259/133‑1 (MQ=255)                                                                                                     tGAAGAGGGCAAACAGCCGGCCACTCAGCTTGATAAAAGCGTCGTTGAATACGCCGGACTGGTGAAGTTCGACTTCCTTGGTTTGCGTACGCTCACCATCATCAACTGGGCGCTGGAGATGATCAACAAgcgg              <  2:263281/133‑1 (MQ=255)                                                                                                            ggCAAACATCCGGCCACCCAGTTTGATAAAAGCGACGTTGAATACGCCGGACTGGTGAAGTTCGACTTCCTTGGTTTGCGTACGCTCACCATCATCAACTTGGCGCTGGAGATGATCGACAAGCGGCGGGCga       >  2:224549/1‑133 (MQ=255)                                                                                                                 aCATCCGGCCACCCAGTTTGATAAAAGCGACGTTGAATACGCCGGACTGGTGAAGTTCGACTTCCTTGGTTTGCGTACGCTCACCATCATCAACTGGGCGCTGGAGATGATCAACAAGCGGCGGGCGAAGAAt  >  1:102530/1‑133 (MQ=255)                                                                                                                         |                                                                                                                             CGCGCAAACTGGAAGGGGTCACCCGTAACGCCGGTAAGCACGCCGGTGGGGTGGTTATCGCGCCGACCAAAATTACCGATTTTGCGCCGCTTTACTGCGATGAAGAGGGCAAACATCCGGTCACCCAGTTTGATAAAAGCGACGTTGAATACGCCGGACTGGTGAAGTTCGACTTCCTTGGTTTGCGTACGCTCACCATCATCAACTGGGCGCTGGAGATGATCAACAAGCGGCGGGCGAAGAAT  >  NC_000913/206618‑206862

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑