Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,260,689	C→T	V39I (GTC→ATC)	dauA ←	aerobic C4‑dicarboxylate transporter DauA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,260,689	0	C	T	100.0%	90.5 / NA	27	V39I (GTC→ATC)	dauA	aerobic C4‑dicarboxylate transporter DauA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (15/12);  total (15/12)

CCCGTCAGAGCAATGACAATCCCCGCAACAGCTGCGGTATATAAACCGTACTGGGGTGCCACACCACTACCAATAGCCAACGCCATCGCCAGCGGGATAGCAATAATCCCGACGGTTATCCCGGCAATCAGGTCACGGGTAAACCGTGCGGCAGTATATTTTTCTTTCCAGCAAGCGTCGATCAGAGCGCGGAAAGGCATCACATGTGAGGAAAATATTTTGTTCACAATAATGTTTC  >  NC_000913/1260577‑1260814                                                                                                                 |                                                                                                                              cccGTCAGAGCAATGACAATCCCCGCAACAGCTGCGGTATATAAACCGTACTGGGGTGCCACACCACTACCAATAGCCAACGCCATCGCCAGCGGGATAGCAATAATCCCGATGGTTATCCCGGCAATCAGGt                                                                                                           >  1:325556/1‑133 (MQ=255)   cGTCAGAGCAATGACAATCCCCGCAACAGCTGCGGTATATAAACCGTACTGGGGTGCCACACCACTACCAATAGCCAACGCCATCGCCAGCGGGATAGCAATAATCCCGATGGTTATCCCGGCAATCAGGTCa                                                                                                         <  2:325556/133‑1 (MQ=255)              tGACAATCCCCGCAACAGCTGCGGTATATAAACCGTACTGGGGTGCCACACCACTACCAATAGCCAACGCCATCGCCAGCGGGATAGCAATAATCCCGATGGTTATCCCGGCAATCAGGTCACGGGTAAACCg                                                                                              >  2:25012/1‑133 (MQ=255)                aCAATCCCCGCAACAGCTGCGGTATATAAACCGTACTGGGGTGCCACACCACTACCAATAGCCAACGCCATCGCCAGCGGGATAGCAATAATCCCGATGGTTATCCCGGCAATCAGGTCACGGGTAAACCGTg                                                                                            <  2:118054/133‑1 (MQ=255)                                cTGCGGTATATAAACCGTACTGGGGTGCCACACCACTACCAATAGCCAACGCCATCGCCAGCGGGATAGCAATAATCCCGATGGTTATCCCGGCAATCAGGTCACGGGTAAACCGTGCGGCAGTATATTtttc                                                                            >  2:587060/1‑133 (MQ=255)                                         ataAACCGTACTGGGGTGCCACACCACTACCAATAGCCAACGCCATCGCCAGCGGGATAGCAATAATCCCGATGGTTATCCCGGCAATCAGGTCACGGGTAAACCGTGCGGCAGTATATTTTTCTTTCCAGCa                                                                   >  2:396707/1‑133 (MQ=255)                                          taAACCGTACTGGGGTGCCACACCACTACCAATAGCCAACGCCATCGCCAGCGGGATAGCAATAATCCCGATGGTTATCCCGGCAATCAGGTCACGGGTAAACCGTGCGGCAGTATATTTTTCTTTCCAGCaa                                                                  <  1:587060/133‑1 (MQ=255)                                               cGTACTGGGGTGCCACACCACTACCAATAGCCAACGCCATCGCCAGCGGGATAGCAATAATCCCGATGGTTATCCCGGCAATCAGGTCACGGGTAAACCGTGCGGCAGTATATTTTTCTTTCCAGCAAGCGTc                                                             >  1:570549/1‑133 (MQ=255)                                                  aCTGGGGTGCCACACCACTACCAATAGCCAACGCCATCGCCAGCGGGATAGCAATAATCCCGATGGTTATCCCGGCAATCAGGTCACGGGTGAACCGTGCCGCAGTATAATTTTCTTACCAGGAAGCGTCGAt                                                          >  2:310867/1‑133 (MQ=255)                                                  aCTGGGGTGCCACACCACTACCAATAGCCAACGCCATCGCCAGCGGGATAGCAATAATCCCGATGGTTATCCCGGCAATCAGGTCACGGGTAAACCGTGCGGCAGTATATTTTTCTTTCCAGCAAGCGTCGAt                                                          >  2:3272/1‑133 (MQ=255)                                                  aCTGGGGTGCCACACCACTACCAATAGCCAACGCCATCGCCAGCGGGATAGCAATAATCCCGATGGTTATCCCGGCAATCAGGTCACGGGTAAACCGTGCGGCAGTATATATTTCTTGCCAGCAAGCGTCGAt                                                          >  1:258594/1‑133 (MQ=255)                                                     ggggTGCCACACCACTACCAATAGCCAACGCCATCGCCAGCGGGATAGCAATAATCCCGATGGTTATCCCGGCAATCAGGTCACGGGTAAACCGTGCGGCAGTATATTTTTCTTTCCAGCAAGCGTCGATCag                                                       >  2:404137/1‑133 (MQ=255)                                                      gggTGCCACACCACTACCAATAGCCAACGCCATCGCCAGCGGGATAGCAATAATCCCGATGGTTATCCCGGCAATCAGGTCACGGGTAAACCGTGCGGCAGTATATTTTTCTTTCCAGCAAGCGTCGATCaga                                                      >  1:542114/1‑133 (MQ=255)                                                             acacCACTACCAATAGCCAACGCCATCGCCAGCGGGATAGCAATAATCCCGATGGTTATCCCGGCAATCAGGTCACGGGTAAACCGTGCGGCAGTATATTTTTCTTTCCAGCAAGCGTCGATCAGAGCGCGGa                                               <  1:555486/133‑1 (MQ=255)                                                               accacTACCAATAGCCAACGCCATCGCCAGCGGGATAGCAATAATCCCGATGGTTATCCCGGCAATCAGGTCACGGGTAAACCGTGCGGCAGTATATTTTTCTTTCCAGCAAGCGTCGATCAGAGCGCGGaaa                                             <  2:570549/133‑1 (MQ=255)                                                                    tACCAATAGCGAACGTCATCGCCATCGGGATAGCAATAATCGCGATGGTTATCCCGGCAATCAGGTCACGGGTAAACCGTGCGGCAGTATATTTTTCTTTCCAGCAAGCGTCGATCAGAGCGCGGAAAGGCAt                                        <  2:258594/133‑1 (MQ=255)                                                                    tACCAATAGCCAACGCCATCGCCAGCGGGATAGCAATAATCCCGATGGTTATCCCGGCAATCAGGTCACGGGTAAACCGTGCGGCAGTATATTTTTCTTTCCAGCAAGCGTCGATCAGAGCGCGGAAAGGCAt                                        <  1:404137/133‑1 (MQ=255)                                                                         aTAGCCAACGCCATCGCCAGCGGGATAGCAATAATCCCGATGGTTATCCCGGCAATCAGGTCACGGGTAAACCGTGCGGCAGTATATTTTTCTTTCCAGCAAGCGTCGATCAGAGCGCGGAAAGGCATCACAt                                   >  2:113788/1‑133 (MQ=255)                                                                           aGCCAACGCCATCGCCAGCGGGATAGCAATAATCCCGATGGTTATCCCGGCAATCAGGTCACGGGTAAACCGTGCGGCAGTATATTTTTCTTTCCAGCAAGCGTCGATCAGAGCGCGGAAAGGCATCACAtgt                                 <  1:396707/133‑1 (MQ=255)                                                                               aaCGCCATCGCCAGCGGGATAGCAATAATCCCGATGGTTATCCCGGCAATCAGGTCACGGGTAAACCGTGCGGCAGTATATTTTTCTTTCCAGCAAGCGTCGATCAGAGCGCGGAAAGGCATCACATGTGAgg                             <  1:25012/133‑1 (MQ=255)                                                                                 cGCCATCGCCAGCGGGATAGCAATAATCCCGATGGTTATCCCGGCAATCAGGTCACGGGTAAACCGTGCGGCAGTATATTTTTCTTTCCAGCAAGCGTCGATCAGAGCGCGGAAAGGCATCACATGTGAGGaa                           >  2:308724/1‑133 (MQ=255)                                                                                 cGCCATCACCAGCGGGATAGCAATAATCCCGATGGTTATCCCGGCAATCAGGTCACGGGTAAACCGTGCGGCAGTATATTTTTCTTTCCAGCAAGCGTCGATCAGAGCGCGGAAAGGCATCACATGTGAGGaa                           >  1:93405/1‑133 (MQ=255)                                                                                            gCGGGATAGCAATAATCCCGATGGTTATCCCGGCAATCAGGTCACGGGTAAACCGTGCGGCAGTATATTTTTCTTTCCAGCAAGCGTCGATCAGAGCGCGGAAAGGCATCACATGTGAGGAAAATATTTTGtt                <  1:308724/133‑1 (MQ=255)                                                                                                  tAGCAATAATCCCGATGGTTATCCCGGCAATCAGGTCACGGGTAAACCGTGCGGCAGTATATTTTTCTTTCCAGCAAGCGTCGATCAGAGCGCGGAAAGGCATCACATGTGAGGAAAATATTTTGTTCACaat          <  1:113788/133‑1 (MQ=255)                                                                                                    gCAATAATCCCGATGGTTATCCCGGCAATCAGGTCACGGGTAAACCGTGCGGCAGTATATTTTTCTTTCCAGCAAGCGTCGATCAGAGCGCAGAAAGGCAGCACTTGTGAGGAAAATATTTTGTTCACaataa        >  2:172087/1‑133 (MQ=255)                                                                                                      aataatCCCGATGGTTATTCCGTCAATCAGCTCACGGGTAAACCGTGCGGCAGTATATTTTTCTTTCCAGCAAGCGTCGATCAGAGCGCGGAAAGGCATCACATGTGAGGAAAATATTTTGTTCACAATAATg      <  1:172087/133‑1 (MQ=255)                                                                                                          atCCCGATGGTTATCCCGGCAATCAGGTCACGGGTAAACCGTGCGGCAGTATATTTTTCTTTCCAGCAAGCGTCGATCAGAGCGCGGAAAGGCATCACATGTGAGGAAAATATTTTGTTCACAATAATGTTTc  >  2:414176/1‑133 (MQ=255)                                                                                                                 |                                                                                                                              CCCGTCAGAGCAATGACAATCCCCGCAACAGCTGCGGTATATAAACCGTACTGGGGTGCCACACCACTACCAATAGCCAACGCCATCGCCAGCGGGATAGCAATAATCCCGACGGTTATCCCGGCAATCAGGTCACGGGTAAACCGTGCGGCAGTATATTTTTCTTTCCAGCAAGCGTCGATCAGAGCGCGGAAAGGCATCACATGTGAGGAAAATATTTTGTTCACAATAATGTTTC  >  NC_000913/1260577‑1260814

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑