Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,279,039	A→G	T361T (ACA→ACG)	narK →	nitrate:nitrite antiporter NarK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,279,039	0	A	G	100.0%	50.9 / NA	15	T361T (ACA→ACG)	narK	nitrate:nitrite antiporter NarK
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (7/8);  total (7/8)

TCGTGTCACGCTGGTGAACTTTATTCTGATGGCGATTTTCAGCGGCCTGCTGTTCCTGACCTTACCGACTGACGGGCAGGGCGGAAGCTTCATGGCGTTCTTCGCGGTCTTCCTGGCGCTGTTCCTGACAGCTGGGCTGGGTAGTGGTTCCACTTTCCAGATGATTTCAGTGATCTTCCGTAAACTGACAATGGATCGCGTGAAAGCAGAAGGGGGTTCTGACGAACGTGCGATGCGTGAAGCGGCAACCGACA  >  NC_000913/1278910‑1279163                                                                                                                                  |                                                                                                                             tCGTGTCATGCTGGTAAACTTTGTTCTGATCGCGATTTTCAGCGGCCTGCTGTTCCTGACCTTACCGACTGACGGGCAGGGCGGAAGCTTCATGGCGTTCTTCGCGGTCTTCCTGGCGCTGTTCCTGACGgct                                                                                                                           <  1:179862/133‑1 (MQ=255)    tgtCACGCTGGTGAACTTTATTCTGATGGCGATTTTCAGCGGCCTGCTGTTCCTGACCTTACCGACTGACGGGCAGGGCGGAAGCTTCATGGCGTTCTTCGCGGTCTTCCTGGCGCTGTTCCTGACGgctggg                                                                                                                        <  2:58205/133‑1 (MQ=255)        aCGCTGGTGAACTTTATTCTGATGGCGATTTTCAGCGGCCTGCTGTTCCTGACCTTACCGACTGACGGGCAGGGCGGAAGCTTCATGGCGTTCTTCGCGGTCTTCCTGGCGCTGTTCCTGACGgctgggctgg                                                                                                                    >  2:546835/1‑133 (MQ=255)          gccggTGAACTTTATTCTGATGGCGATTTTCAGCGGCCTGCTGTTCCTGACCTTACCGACTGACGGGCAGGGCGGAAGCTTCATGGCGTTCTTCGCGGTCTTCCTGGCGCTGTTCCTGACGGCTGGGCTGGGt                                                                                                                  <  1:334816/130‑1 (MQ=255)          gCTGGTGAACTTTATTCTGATGGCGATTTTCAGCGGCCTGCTGTTCCTGACCTTACCGACTGACGGGCAGGGCGGAAGCTTCATGGCGTTCTTCGCGGTCTTCCTGGCGCTGTTCCTGACGGCTGGGCTGGGt                                                                                                                  <  1:595411/133‑1 (MQ=255)                    tttATTCTGATGGCGATTTTCAGCGGCCTGCTGTTCCTGACCTTACCGACTGACGGGCAGGGCGGAAGCTTCATGGCGTTCTTCGCGGTCTTCCTGGCGCTGTTCCTGACGGCTGGGCTGGGTAGTGGTTCCa                                                                                                        <  2:361413/133‑1 (MQ=255)                      tATTCTGATGGCGATTTTCAGCGGCCTGCTGTTCCTGACCTTACCGACTGACGGGCAGGGCGGAAGCTTCATGGCGTTCTTCGCGGTCTTCCTGGCGCTGTTCCTGACGGCTGGGCTGGGTAGTGGTTCCACt                                                                                                      >  2:327930/1‑133 (MQ=255)                             aTGGCGATTTTCAGCGGCCTGCTGTTCCTGACCTTACCGACTGACGGGCAGGGCGGAAGCTTCATGGCGTTCTTCGCGGTCTTCCTGGCGCTGTTCCTGACGGCTGGGCTGGGTAGTGGTTCCACTTTCCAga                                                                                               >  1:617124/1‑133 (MQ=255)                              tGGCGATTTTCAGCGGCCTGCTGTTCCTGACCTTACCGACTGACGGGCAGGGCGGAAGCTTCATGGCGTTCTTCGCGGTCTTCCTGGCGCTGTTCCTGACGGCTGGGCTGGGTAGTGGTTCCACTTTCCAgat                                                                                              <  2:594848/133‑1 (MQ=255)                                              ccTGCTGTTCCTGACCTTACCGACTGACGGGCAGGGCGGAAGCTTCATGGCGTTCTTCGCGGTCTTCCTGGCGCTGTTCCTGACGGCTGGGCTGGGTAGTGGTTCCACTTTCCAGATGATTTCAGTGATCTTc                                                                              >  2:507462/1‑133 (MQ=255)                                                             cTTACCGACTGACGGGCAGGGCGGAAGCTTCATGGCGTTCTTCGCGGTCTTCCTGGCGCTGTTCCTGACGGCTGGGCTGGGTAGTGGTTCCACTTTCCAGATGATTTCAGTGATCTTCCGTAAACTGACAATg                                                               >  2:352686/1‑133 (MQ=255)                                                                aCCGACTGACGGGCAGGGCGGAAGCTTCATGGCGTTCTTCGCGGTCTTCCTGGCGCTGTTCCTGACGGCTGGGCTGGGTAGTGGTTCCACTTTCCAGATGATTTCAGTGATCTTCCGTAAACTGACAATGGAt                                                            >  2:506627/1‑133 (MQ=255)                                                                  cGACTGACGGGCAGGGCGGAAGCTTCATGGCGTTCTTCGCGGTCTTCCTGGCGCTGTTCCTGACGGCTGGGCTGGGTAGTGGTTCCACTTTCCAGATGATTTCAGTGATCTTCCGTAAACTGACAATGGATcg                                                          <  1:327930/133‑1 (MQ=255)                                                                                 gCGGAAGCTTCATGGTGTCCTTCGCGGTCTCCCTGGCGCTGTTCCTGACGGCTGGGCTGGGTAGTGGTTCCACTTTCCAGATGATTTCAGTGATCTTCCGTAAACTGACAATGGATCGCGTGAAAGCAGAAgg                                           <  1:251209/133‑1 (MQ=255)                                                                                                                          ttCCTGACGGCTGGGCTGGGTAGTGGTTCCACTTTCCAGATGATTTCAGTGATCTTCCGTAAACTGACAATGGATCGTGTGAAAGCAGAAGGGGGTTCTGACGAACGTGCGATGCGTGAAGCGGCAACCGaca  >  2:440627/1‑133 (MQ=255)                                                                                                                                  |                                                                                                                             TCGTGTCACGCTGGTGAACTTTATTCTGATGGCGATTTTCAGCGGCCTGCTGTTCCTGACCTTACCGACTGACGGGCAGGGCGGAAGCTTCATGGCGTTCTTCGCGGTCTTCCTGGCGCTGTTCCTGACAGCTGGGCTGGGTAGTGGTTCCACTTTCCAGATGATTTCAGTGATCTTCCGTAAACTGACAATGGATCGCGTGAAAGCAGAAGGGGGTTCTGACGAACGTGCGATGCGTGAAGCGGCAACCGACA  >  NC_000913/1278910‑1279163

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑