Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,297,007	T→C	Y372C (TAC→TGC)	adhE ←	aldehyde‑alcohol dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,297,007	0	T	C	100.0%	35.0 / NA	11	Y372C (TAC→TGC)	adhE	aldehyde‑alcohol dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (8/3);  total (8/3)

GGTGCGAGTTTGAAGTTATACAGGTCACCGATACCACCCTGAGACGCTGGGGTGTTAATCAGGATACGCGCCGTTTTCATTTTCTGACCGAAGTAAGAAACGCGAGCCGGTTGGTTATCCTGGTCAGTGTACAGGCAAGAGGTATGACCGATACCGCCCATAGCAACCAGTTTCTCTGCTTTTTCTACCGCGTCTTCGAAATCTTTAGCGCGGTACATTGCCAGAGTCG  >  NC_000913/1296878‑1297106                                                                                                                                  |                                                                                                    ggTGCGAGTTTGAAGTTATACAGGTCACCGATACCACCCTGAGACGCTGGGGTGTTAATCAGGATACGCGCCGTTTTCATTTTCTGACCGAAGTAAGAAACGCGAGCCGGTTGGTTATCCTGGTCAGTGcaca                                                                                                  >  2:555104/1‑133 (MQ=255)      gAGTTTGAAGTTATACAGGTCACCGATACCACCCTGAGACGCTGGGGTGTTAATCAGGATACGCGCCGTTTTCATTTTCTGACCGAAGTAAGAAACGCGAGCCGGTTGGTTATCCTGGTCAGTGCACAGGCaa                                                                                             <  1:408354/133‑1 (MQ=255)                          cACCGATACCACCCTGAGACGCTGGGGTGTTAATCAGGATACGCGCCGTTTTCATTTTCTGACCGAAGTAAGAAACGCGAGCCGGTTGGTTATCCTGGTCAGTGCACAGGCAAGAGGTATGACCGATACCGcc                                                                         >  1:636210/1‑133 (MQ=255)                                          agaCGCTGGGGTGTTAATCAGGATACGCGCCGTTTTCATTTTCTGACCGAAGTAAGAAACGCGAGCCGGTTGGTTATCCTGGTCAGTGCACAGGCAAGAGGTATGACCGATACCGCCCATAGCAACCAGTTtc                                                         >  1:386407/1‑133 (MQ=255)                                                                        cGTTTTCATTTTCTGACCGAAGTAAGAAACGCGAGCCGGTTGGTTATCCTGGTCAGTGCACAGGCAAGAGGTATGACCGATACCGCCCATAGCAACCAGTTTCTCTGCTTTTTCTACCGCGTCTTCGAAATCt                           >  2:383898/1‑133 (MQ=255)                                                                         gTTTTCATTTTCTGACCGAAGTAAGAAACGCGAGCCGGTTGGTTATCCTGGTCAGTGCACAGGCAAGAGGTATGACCGATACCGCCCATAGCAACCAGTTTCTCTGCTTTTTCTACCGCGTCTTCGAAATCtt                          >  2:610141/1‑133 (MQ=255)                                                                              cATTTTCTTACCGAAATACGAAGCGCGAGCCGGTTGGTTATCCTGGTCAGTGCACAGGCAAGAGGTATGACCGATACCGCCCATAGCAACCAGTTTCTCTGCTTTTTCTACCGCGTCTTCGAAATCTTTAgcg                     <  1:183480/133‑1 (MQ=255)                                                                                  ttCTGACCGAAGTAAGAAACGCGAGCCGGTTGGTTATCCTGGTCAGTGCACAGGCAAGAGGTATGACCGATACCGCCCATAGCAACCAGTTTCTCTGCTTTTTCTACCGCGTCTTCGAAATCTTTAGCGCGGt                 >  2:131717/1‑133 (MQ=255)                                                                                    cTGACCGAAGTAAGAAACGCGAGCCGGTTGGTTATCCTGGTCAGTGCACAGGCAAGAGGTATGACCGATACCGCCCATAGCAACCAGTTTCTCTGCTTTTTCTACCGCGTCTTCGAAATCTTTAGCGCGGTAc               >  1:370181/1‑133 (MQ=255)                                                                                       aCCGAAGTAAGAAACGCGAGCCGGTTGGTTATCCTGGTCAGTGCACAGGCAAGAGGTATGACCGATACCGCCCATAGCAACCAGTTTCTCTGCTTTTTCTACGGCGTCTTGGAAGTCATTAGCGAGGTACAtt            >  1:186124/1‑133 (MQ=255)                                                                                         cGAAGTAAGAAACGCGAGCCGGTTGGTTATCCTGGTCAGTGCACAGGCAAGAGGTATGACCGATACCGCCCATAGCAACCAGTTTCTCTGCTTTTTCTACCGCGTCTTCGAAATCTTTAGCGCGGTACATTGc          <  2:370181/133‑1 (MQ=255)                                                                                                 gAAACGCGAGCCGGTTGGTTATCCTGGTCAGTGCACAGGCAAGAGGTATGACCGATACCGCCCATAGCAACCAGTTTCTCTGCTTTTTCTACCGCGTCTTCGAAATCTTTAGCGCGGTACATTGCCAGAGTCg  >  1:114720/1‑133 (MQ=255)                                                                                                                                  |                                                                                                    GGTGCGAGTTTGAAGTTATACAGGTCACCGATACCACCCTGAGACGCTGGGGTGTTAATCAGGATACGCGCCGTTTTCATTTTCTGACCGAAGTAAGAAACGCGAGCCGGTTGGTTATCCTGGTCAGTGTACAGGCAAGAGGTATGACCGATACCGCCCATAGCAACCAGTTTCTCTGCTTTTTCTACCGCGTCTTCGAAATCTTTAGCGCGGTACATTGCCAGAGTCG  >  NC_000913/1296878‑1297106

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑