Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,387,918	C→T	A400V (GCC→GTC)	tyrR →	DNA‑binding transcriptional dual regulator TyrR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,387,918	0	C	T	100.0%	71.2 / NA	23	A400V (GCC→GTC)	tyrR	DNA‑binding transcriptional dual regulator TyrR
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (7/16);  total (7/16)

TTATCGTCTGAACGTGTTGACGCTCAATCTGCCGCCGCTACGTGACTGTCCGCAGGACATCATGCCGTTAACTGAGCTGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTCCGAAACTGGCCGCTGACCTGAATACTGTACTTACGCGTTATGCGTGGCCGGGAAATGTGCGGCAGTTAAAGAACGCTATCTATCGCGCACTGACACAACTGGACGGTTATGAGCTGCGTCCACAGG  >  NC_000913/1387790‑1388034                                                                                                                                 |                                                                                                                     ttatCGTCTGTACGTGTTGACGCTTAATTTGCCGCCGCTACTTGACTGTCCGCAGGACATCATGCCGTTAACTGAGCTGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTCCGAAACTGGTCGCt                                                                                                                  <  2:178031/133‑1 (MQ=255) ttatCGTCTGAACGTTTTGACGTTCAATCTGCCGCCGCTACGTGACTGTCCGCAGGACATCATGCCGTTAACTGAGCTGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTCCGAAACTGGTCGCt                                                                                                                  <  1:254323/133‑1 (MQ=255) ttatCGTCTGAACGTGTTGACGCTAAATGTGCCGCCGCTACGTGACTGTCCGCAGGACATCATGCCGTTAACTGAGCTGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTCCGAAACTGGTCGCt                                                                                                                  <  2:260589/133‑1 (MQ=255)   atCGTCTGAACGTGTTGACGCTCAATCTGCCGCCGCTACGTGACTGTCCGCAGGAC‑TCATGCCGTTAACTGAGCTGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTCCGAAACTGGTCGCTGAc                                                                                                               <  2:377112/133‑1 (MQ=255)    tCGTTTGAACGTGTTGACGCTCAATCTGCCGCCGCTACGTGACTGTCCGCAGGACATCATGCCGTTAACTGAGCTGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTCCGAAACTGGTCg                                                                                                                    <  1:331075/128‑1 (MQ=255)    tCGTCTGAACGTGTTGACGCTCAATCTGCCGCCGCTACGTGACTGTCCGCAGGACATCATGCCGTTAACTGAGCTGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTCCGAAACTGGTCg                                                                                                                    >  2:331075/1‑128 (MQ=255)    tCGTCTGAACGTGTTGACGCTCAATCTGCCGCCGCTACGTGACTGTCCGCAGGACATCATGCCGTTAACTGAGCTGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTCCGAAACTGGTCGCTGAc                                                                                                               <  2:90899/133‑1 (MQ=255)      gTCTGAACGTGTTGACGCTCAATCTGCCGCCGCTACGTGACTGTCCGCAGGACATCATGCCGTTAACTGAGCTGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTCCGAAACTGGTCGCTGACCt                                                                                                             >  1:427407/1‑133 (MQ=255)      gTCTGAACGTGTTGACGCTCAATCTGCCGCCGCTACGTGACTGTCCGCAGGACATCATGCCGTTAACTGAGCTGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTCCGAAACTGGTCGCTGACCt                                                                                                             <  2:102180/133‑1 (MQ=255)       tCTGAACGTGTTGACGCTCAATCTGCCGCCGCTACGTGACTGTCCGCAGGACATCATGCCGTTAACTGAGCTGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTCCGAAACTGGTCGCTGACCTg                                                                                                            >  2:461400/1‑133 (MQ=255)         tGAACGTGTTGACGCTCAATCTGCCGCCGCTACGTGACTGTCCGCAGGACATCATGCCGTTAACTGAGCTGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTCCGAAACTGGTCGCTGACCTGaa                                                                                                          <  1:461400/133‑1 (MQ=255)           aaCGTGTTGACGCTCAATCTGCCGCCGCTACGTGACTGTCCGCAGGACATCATGCCGTTAACTGAGCTGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGAGCCGCGTGCGAAACTGGTCGCCGACCTGAATa                                                                                                        >  2:254323/1‑133 (MQ=255)                        tCAATCTGCCCCCGCAACGTGACTGTCCTCAGGACATCATGACGTTAACTGAGCTGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTCCGAAACTGGTCGCTGACCTGAATACTGTACTTAcgcg                                                                                           <  2:215060/133‑1 (MQ=255)                               gccgccGCTACGTGACTGTCCGCACGACATCAGGCCGTTAACTGAGCTGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTCCGAAACTGGTCGCTGACCTGAATACTGTACTTACGCGTTATGCg                                                                                    <  2:226730/133‑1 (MQ=255)                                               tGTCCGCAGGACATCATGCCGTTAACTGAGCTGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTCCGAAACTGGTCGCTGACCTGAATACTGTACTTACGCGTTATGCGTGGCCGGGAAATGTGc                                                                    >  2:381549/1‑133 (MQ=255)                                                 tCCGCAGGACATCATGCCGTTAACTGAGCTGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTCCGAAACTGGTCGCTGACCTGAATACTGTACTTACGCGTTATGCGTGGCCGGGAAATGTGCgg                                                                  <  1:403253/133‑1 (MQ=255)                                                 tCCGCAGGACATCATGCCGTTAACTGAGCTGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTCCGAAACTGGTCGCTGACCTGAATACTGTACTTACGCGATATGCGTGGCCGGGAAATGTGCgg                                                                  <  2:427407/133‑1 (MQ=255)                                                                           aGCTGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTCCGAAACTGGTCGCTGACCTGAATACTGTACTTACGCGTTATGCGTGGCCGGGAAATGTGCGGCAGTTAAAGAACGCTATCTATCgcgc                                        <  1:527776/133‑1 (MQ=255)                                                                             cTGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTCCGAAACTGGTCGCTGACCTGAATACTGTACTTACGCGTTATGCGTGGCCGGGAAATGTGCGGCAGTTAAAGAACGCTATCTATCGCGCAc                                      >  2:525926/1‑133 (MQ=255)                                                                                                   acgaGCAGGGCGTGCCGCGTCCGAAACTGGTCGCTGACCTGAATACTGTACTTACGCGTTATGCGTGGCCGGGAAATGTGCGGCAGTTAAAGAACGCTATCTATCGCGCACTGACACAACTGGACGGTTATGa                <  1:329290/133‑1 (MQ=255)                                                                                                              gTGCCGCGTCTGAAACTGGTCGCTAACCTGAATACTGTACTTACGCGTTATGCGTGGCCGGGAAATGTGCGGCAGTTAAAGAACGCTATCTATCGCGCACTGACACAACTGGACGGTTATGAGCTGCGTCcac     <  2:301525/133‑1 (MQ=255)                                                                                                               tGCCGCGTCCGAAACTGGTCGCTGACCTGAATACTGTACTTACGCGTTATGCGTGGCCGGGAAATGTGCGGCAGTTAAAGAACGCTATCTATCGCGCACTGACACAACTGGACGGTTATGAGCTGCGTCcaca    <  1:419903/133‑1 (MQ=255)                                                                                                                 ccGCGTCCGAAACTGGTCGCTGACCTGAATACTGTACTTACGCGTTATGCGTGGCCGGGAAATGTGCGGCAGTTAAAGAACGCTATCTATCACGCACTGATACAGCTGGACGGTTATAAGCTGAGTCCACAgg  >  2:289370/1‑133 (MQ=255)                                                                                                                                 |                                                                                                                     TTATCGTCTGAACGTGTTGACGCTCAATCTGCCGCCGCTACGTGACTGTCCGCAGGACATCATGCCGTTAACTGAGCTGTTCGTCGCCCGCTTTGCCGACGAGCAGGGCGTGCCGCGTCCGAAACTGGCCGCTGACCTGAATACTGTACTTACGCGTTATGCGTGGCCGGGAAATGTGCGGCAGTTAAAGAACGCTATCTATCGCGCACTGACACAACTGGACGGTTATGAGCTGCGTCCACAGG  >  NC_000913/1387790‑1388034

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑