Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,442,689	A→G	V52A (GTA→GCA)	ldhA ←	D‑lactate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,442,689	0	A	G	100.0%	176.5 / NA	48	V52A (GTA→GCA)	ldhA	D‑lactate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (26/22);  total (26/22)

ACTACTTTCAGCCCCAGTTCTTTTGCCGCGTCAAGGTCGACGTTATTGAAACCGGCACAGCGCAGGGCGATATATTTAACGCCGTGCTTTTTCAGCTCTTCCAGCACCGGGCGGCTGCCGTCATCGTTTACGAAAATACATACCGCTTCGCAGCCATTGGCAGTTTTAGCGGTTTTTTCCGTCAGCAGAAAGTCAAAAAATTCCAGCTCAAAGCCAAAGGACTCGTTCACCTGTTGCAGGTACTTCTTGTCGTACTGTT  >  NC_000913/1442560‑1442818                                                                                                                                  |                                                                                                                                  actactTTCAGCCCCAGTTCTTTTGCCGCGTCAAGGTCGACGTTATTGAAACCGGCACAGCGCAGGGCGATATATTTAACGCCGTGCTTTTTCAGCTCTTCCAGCACCGGGCGGCTGCCGTCATCGTTTGCGa                                                                                                                                <  2:584161/133‑1 (MQ=255)   tactTTCAGCCCCAGTTCTTTTGCCGCGTCAAGGTCGACGTTATTGAAACCGGCACAGCGCAGGGCGATATATTTAACGCCGTGCTTTTTCAGCTCTTCCAGCACCGGGCGGCTGCCGTCATCGTTTGCGaaa                                                                                                                              <  2:484153/133‑1 (MQ=255)      tttCAGCCCCAGTTCTTTTGCCGCGTCAAGGTCGACGTTATTGAAACCGGCACAGCGCAGGGCGATATATTTAACGCCGTGCTTTTTCAGCTCTTCCAGCACCGGGCGGCTGCCGTCATCGTTTGCGAAAata                                                                                                                           >  2:463110/1‑133 (MQ=255)          aGCCCCAGTTCTTTTGCCGCGTCAAGGTCGACGTTATTGAAACCGGCACAGCGCAGGGCGATATATTTAACGCCGTGCTTTTTCAGCTCTTCCAGCACCGGGCGGCTGCCGTCATCGTTTGCg                                                                                                                                 >  2:491545/1‑123 (MQ=255)          aGCCCCAGTTCTTTTGCCGCGTCAAGGTCGACGTTATTGAAACCGGCACAGCGCAGGGCGATATATTTAACGCCGTGCTTTTTCAGCTCTTCCAGCACCGGGAGGCTGCCATCACCGATTGCGATAatacata                                                                                                                       >  1:284660/1‑133 (MQ=255)               cAGTTCTTTTGCCGCGTCAAGGTCGACGTTATTGAAACCGGCACAGCGCAGGGCGATATATTTAACGCCGTGCTTTTTCAGCTCTTCCAGCACCGGGCGGCTGCCGTCATCGTTTGCGAAAATACATACCGCt                                                                                                                  >  2:526117/1‑133 (MQ=255)               cAGTTCTTTTGCCGCGTCAAGGTCGACGTTATTGAAACCGGCACAGCGCAGGGCGATATATTTAACGCCGTGCTTTTTCAGCTCTTCCAGCACCGGGCGGCTGCCGTCATCGTTTGCGAAAATACATACCGCt                                                                                                                  >  1:32323/1‑133 (MQ=255)                        tGCCGCGTCAAGGTCGACGTTATTGAAACCGGCACAGCGCAGGGCGATATATTTAACGCCGTGCTTTTTCAGCTCTTCCAGCACCGGGCGGCTGCCGTCATCGTTTGCGAAAATACATACCGCTTCGCAGCCa                                                                                                         <  1:459480/133‑1 (MQ=255)                          ccGCGTCAAGGTCGACGTTATTGAAACCGGCACAGCGCAGGGCGATATATTTAACGCCGTGCTTTTTCAGCTCTTCCAGCACCGGGCGGCTGCCGTCATCGTTTGCGAAAATACATACCGCTTCGCAGCCAtt                                                                                                       >  1:72725/1‑133 (MQ=255)                          ccGCGTCAAGGTCGACGTTATTGAAACCGGCACAGCGCAGGGCGATATATTTAACGCCGTGCTTTTTCAGCTCTTCCAGCACCGGGCGGCTGCCGTCATCGTTTGCGAAAATACATACCGCTTCGCAGCCAtt                                                                                                       >  2:430609/1‑133 (MQ=255)                                           ttATTGAAACCGGCACAGCGCAGGGCGATATATTTAACGCCGTGCTTTTTCAGCTCTTCCAGCACCGGGCGGCTGCCGTCATCGTTTGCGAAAATACATACCGCTTCGCAGCCATTGGCAGTTTTAGCGGttt                                                                                      <  2:523165/133‑1 (MQ=255)                                                   aCCGGCACAGCGCAGGGCGATATATTTAACGCCGTGCTTTTTCAGCTCTTCCAGCACCGGGCGGCTGCCGTCATCGTTTGCGAAAATACATACCGCTTCGCAGCCATTGGCAGTTTTAGCGGTTTTTTCCGTc                                                                              <  1:463110/133‑1 (MQ=255)                                                        cacaGCGCAGGGCGATATATTTAACGCCGTGCTTTTTCAGCTCTTCCAGCACCGGGCGGCTGCCGTCATCGTTTGCGAAAATACATACCGCTTCGCAGCCATTGGCAGTTTTAGCGGTTTTTTCCGTcagcag                                                                         >  1:603003/1‑133 (MQ=255)                                                               cAGGGCGATATATTTTACGCCGCGCTTTTTCAGCTCTTCCAGCACCGGGCGGCTGCCGTCATCGTTTGCGAAAATACATACCGCTTCGCAGCCATTGGCAGTTTTAGCGGTTTTTTCCGTCAGCAGAAAGTCa                                                                  <  1:217215/133‑1 (MQ=255)                                                                  ggCGATATATTTAACGCCGTGCTTTTTCAGCTCTTCCAGCACCGGGCGGCTGCCGTCATCGTTTGCGAAAATACATACCGCTTCGCAGCCATTGGCAGTTTTAGCGGTTTTTTCCGTCAGCAGAAAGTCaaaa                                                               >  2:472822/1‑133 (MQ=255)                                                                  ggCGATATATTTAACGCCGTGCTTTTTCAGCTCTTCCAGCACCGGGCGGCTGCCGTCATCGTTTGCGAAAATACATACCGCTTCGCAGCCATTGGCAGTTTTAGCGGTTTTTTCCGTCAGCAGAAAGTCaaaa                                                               >  2:394879/1‑133 (MQ=255)                                                                       tatatTTAACGCCGTGCTTTTTCAGCTCTTCCAGCACCGGGCGGCTGCCGTCATCGTTTGCGAAAATACATACCGCTTCGCAGCCATTGGCAGTTTTAGCGGTTTTTTCCGTCAGCAGAAAGTCAAAAAATTc                                                          >  1:525218/1‑133 (MQ=255)                                                                            ttAACGCCGTGCTTTTTCAGCTCTTCCAGCACCGGGCGGCTGCCGTCATCGTTTGCGAAA‑‑‑‑ATACCGCTTCGCAGCCATTGGCAGTTTTAGCGGTTTTTTCCGTCAGCAGAAAGTCAAAAAATTCCAGCTCaaa                                                 >  2:520122/1‑133 (MQ=255)                                                                              aaCGCCGTGCTTTTTCAGCTCTTCCAGCACCGGGCGGCTGCCGTCATCGTTTGCGAAAATACATACCGCTTCGCAGCCATTGGCAGTTTTAGCGGTTTTTTCCGTCAGCAGAAAGTCAAAAAATTCCAGCTCa                                                   >  2:419093/1‑133 (MQ=255)                                                                               aCGCCGTGCTTTTTCAGCTCTTCCAGCACCGGGCGGCTGCCGTCATCGTTTGCGAAAATACATACCGCTTCGCAGCCATTGGCAGTTTTAGCGGTTTTTTCCGTCAGCAGAAAGTCAAAAAATTCCAGCTCaa                                                  <  1:394879/133‑1 (MQ=255)                                                                                cGCCGTGCTTTTTCAGCTCTTCCAGCACCGGGCGGCTGCCGTCATCGTTTGCGAAAATACATACCGCTTCGCAGCCATTGGCAGTTTTAGCGGTTTTTTCCGTCAGCAGAAAGTCAAAAAATTCCAGCTCaaa                                                 <  1:472822/133‑1 (MQ=255)                                                                                 gCCGTGCTTTTTCAGCTCTTCCAGCACCGGGCGGCTGCCGTCATCGTTTGCGAAAATACATACCGCTTCGCAGCCATTGGCAGTTTTAGCGGTTTTTTCCGTCAGCAGAAAGTCAAAAAATTCCAGCTCAAAg                                                >  2:630830/1‑133 (MQ=255)                                                                                    gTGCTTTTTCAGCTCTTCCAGCACCGGGCGGCTGCCGTCATCGTTTGCGAAAATACATACCGCTTCGCAGCCATTGGCAGTTTTAGCGGTTTTTTCCGTCAGCAGAAAGTCAAAAAATTCCAGCTCAAAGCCa                                             >  2:423320/1‑133 (MQ=255)                                                                                     tGCTTTTTCAGCTCTTCCAGCACCGGGCGGCTGCCGTCATCGTTTGCGAAAATACATACCGCTTCGCAGCCATTGGCAGTTTTAGCGGTTTTTTCCGTCAGCAGAAAGTCAAAAAATTCCAGCTCAAAGCCaa                                            >  1:16277/1‑133 (MQ=255)                                                                                              aGCTCTTCCAGCACCGGGCGGCTGCCGTCATCGTTTGCGAAAATACATACCGCTTCGCAGCCATTGGCAGTTTTAGCGGTTTTTTCCGTCAGCAGAAAGTCAAAAAATTCCAGCTCAAAGCCAAAGGACTCGt                                   <  2:32323/133‑1 (MQ=255)                                                                                              aGCTCTTCCAGCACCGGGCGGCTGCCGTCATCGTTTGCGAAAATACATACCGCTTCGCAGCCATTGGCAGTTTTAGCGGTTTTTTCCGTCAGCAGAAAGTCAAAAAATTCCAGCTCAAAGCCAAAGGACTCGt                                   <  1:423320/133‑1 (MQ=255)                                                                                               gCTCTTCCAGCACCGGGCGGCTGCCGTCATCGTTTGCGAAAATACATACCGCTTCGCAGCCATTGGCAGTTTTAGCGGTTTTTTCCGTCAGCAGAAAGTCAAAAAATTCCAGCTCAAAGCCAAAGGACTCGtt                                  <  1:419093/133‑1 (MQ=255)                                                                                                ctctTCCAGCACCGGGCGGCTGCCGTCATCGTTTGCGAAA‑‑‑‑ATACCGCTTCGCAGCCATTGGCAGTTTTAGCGGTTTTTTCCGTCAGCAGAAAGTCAAAAAATTCCAGCTCAAAGCCAAAGGACTCGTTCACCt                             <  1:520122/133‑1 (MQ=255)                                                                                                    tCCAGCACCGGGCGGCTGCCGTCATCGTTTGCGAAAATACATACCGCTTCGCAGCCATTGGCAGTTTTAGCGGTTTTTTCCGTCAGCAGAAAGTCAAAAAATTCCAGCTCAAAGCCAAAGGACTCGTTCACCt                             >  1:460418/1‑133 (MQ=255)                                                                                                             gggCGGCTGCCGTCATCGTTTGCGAAAATACATACCGCTTCGCAGCCATTGGCAGTTTTAGCGGTTTTTTCCGTCAGCAGAAAGTCAAAAAATTCCAGCTCAAAGCCAAAGGACTCGTTCACCTGTTGCAGGt                    >  1:104154/1‑133 (MQ=255)                                                                                                              ggcggcTGCCGTCATCGTTTGCGAAAATACATACCGCTTCGCAGCCATTGGCAGTTTTAGCGGTTTTTTCCGTCAGCAGAAAGTCAAAAAATTCCAGCTCAAAGCCAAAGGACTCGTTCACCTGtt                          >  2:630760/1‑126 (MQ=255)                                                                                                              ggcggcTGCCGTCATCGTTTGCGAAAATACATACCGCTTCGCAGCCATTGGCAGTTTTAGCGGTTTTTTCCGTCAGCAGAAAGTCAAAAAATTCCAGCTCAAAGCCAAAGGACTCGTTCACCTGtt                          <  1:630760/126‑1 (MQ=255)                                                                                                              ggcggcTGCCGTCATCGTTTGCGAAAATACATACCGCTTCGCAGCCATTGGCAGTTTTAGCGGTTTTTTCCGTCAGCAGAAAGTCAAAAAATTCCAGCTCAAAGCCAAAGGACTCGTTCACCTGTTGCAGGTa                   >  1:65576/1‑133 (MQ=255)                                                                                                              ggcggcTGCCGTCATCGTTTGCGAAAATACATACCGCTTCGCAGCCATTGGCAGTTTTAGCGGTTTTTTCCGTCAGCAGAAAGTCAAAAAATTCCAGCTCAAAGCCAAAGGACTCGTTCACCTGTTGCAGGTa                   >  1:26417/1‑133 (MQ=255)                                                                                                              ggcggcTGCCGTCATCGTTTGCGAAAATACATACCGCTTCGCAGCCATTGGCAGTTTTAGCGGTTTTTTCCGTCAGCAGAAAGTCAAAAAATTCCAGCTCAAAGCCAAAGGACTCGTTCACCTGTTGCAGGTa                   >  2:459469/1‑133 (MQ=255)                                                                                                              ggcggcTGCCGTCATCGTTTGCGAAAATACATACCGCTTCGCAGCCATTGGCAGTTTTAGCGGTTTTTTCCGTCAGCAGAAAGTCAAAAAATTCCAGCTCAAAGCCAAAGGACTCGTTCACCTGTTGCAGGTa                   >  1:614436/1‑133 (MQ=255)                                                                                                              ggcggcTGCCGTCATCGTTTGCGAAAATACATACCGCTTCGCAGCCATTGGCAGTTTTAGCGGTTTTTTCCGTCAGCAGAAAGTCAAAAAATTCCAGCTCAAAGCCAAAGGACTCGTTCACCTGTTGCAGGTa                   >  1:617018/1‑133 (MQ=255)                                                                                                               gcggcTGCCGTCATCGTTTGCGAAAATACATACCGCTTCGCAGCCATTGGCAGTTTTAGCGGTTTTTTCCGTCAGCAGAAAGTCAAAAAATTCCAGCTCAAAGCCAAAGGACTCGTTCACCTGTTGCAGGTAc                  >  2:528688/1‑133 (MQ=255)                                                                                                                cggcTGCCGTCATCGTTTGCGAAAATACATACCGCTTCGCAACCATTGGCAGTTTTAGCGGTTTTTTCCGTCAGCAGAAAGTCAAAAAATTCCAGCTCAAAGCCAAAGGACTCGTTCACCTGTTGCAGGTAct                 <  1:526117/133‑1 (MQ=255)                                                                                                                  gcTGCCGTCATCGTTTGCGAAAATACATACCGCTTCGCAGCCATTGGCAGTTTTAGCGGTTTTTTCCGTCAGCAGAAAGTCAAAAAATTCCAGCTCAAAGCCAAAGGACTCGTTCACCTGTTGCAGGTActtc               <  2:603003/133‑1 (MQ=255)                                                                                                                     gCCGTCATCGTTTGCGAAAATACATACCGCTTCGCAGCCATTGGCAGTTTTAGCGGTTTTTTCCGTCAGCAGAAAGTCAAAAAATTCCAGCTCAAAGCCAAAGGACTCGTTCACCTGTTGCAGGTACTTCTTg            <  2:488091/133‑1 (MQ=255)                                                                                                                     gCCGTCATCGTTTGCGAAAATACATACCGCTTCGCAGCCATTGGCAGTTTTAGCGGTTTTTTCCGTCAGCAGAAAGTCAAAAAATTCCAGCTCAAAGCCAAAGGACTCGTTCACCTGTTGCAGGTACTTCTTg            <  1:528688/133‑1 (MQ=255)                                                                                                                     gCCGTCATCGTTTGCGAAAATACATACCGCTTCGCAGCCATTGGCAGTTTTAGCGGTTTTTTCCGTCAGCAGAAAGTCAAAAAATTCCAGCTCAAAGCCAAAGGACTCGTTCACCTGTTGCAGGTACTTCTTg            <  1:630830/133‑1 (MQ=255)                                                                                                                         tCATCGTTTGCGAAAATACATACCGCTTCGCAGCCATTGGCAGTTTTAGCGGTTTTTTCCGTCAGCAGAAAGTCAAAAAATTCCAGCTCAAAGCCAAAGGACTCGTTCACCTGTTGCAGGTACTTCTTGTCGt        >  2:517733/1‑133 (MQ=255)                                                                                                                         tCATCGTTTGCGAAAATACATACCGCTTCGCAGCCATTGGCAGTTTTAGCGGTTTTTTCCGTCAGCAGAAAGTCAAAAAATTCCAGCTCAAAGCCAAA‑GACTCGTTCACCTGTTGCAGGTACTTCTTGTCGTa       <  2:525218/133‑1 (MQ=255)                                                                                                                          cATCGTTTGCGAAAATACATACCGCTTCGCAGCCATTGGCAGTTTTAGCGGTTTTTTCCGTCAGCAGAAAGTCAAAAAATTCCAGCTCAAAGCCAAAGGACTCGTTCACCTGTTGCAGGTACTTCTTGTCGTa       <  2:72725/133‑1 (MQ=255)                                                                                                                            tCGTTTGCGAAAATACATACCGCTTCGCAGCCATTGGCAGTTTTAGCGGTTTTTTCCGTCAGCAGAAAGTCAAAAAATTCCAGCTCAAAGCCAAAGGACTCGTTCACCTGTTGCAGGTACTTCTTGTCGTACt     <  2:16277/133‑1 (MQ=255)                                                                                                                               tttGCGAAAATACATACCGCTTCGCAGCCATTGGCAGTTTTAGCGGTTTTTTCCGTCAGCAGAAAGTCAAAAAATTCCAGCTCAAAGCCAAAGGACTCGTTCACCTGTTGCAGGTACTTCTTGTCGTACTGtt  <  2:104154/133‑1 (MQ=255)                                                                                                                                  |                                                                                                                                  ACTACTTTCAGCCCCAGTTCTTTTGCCGCGTCAAGGTCGACGTTATTGAAACCGGCACAGCGCAGGGCGATATATTTAACGCCGTGCTTTTTCAGCTCTTCCAGCACCGGGCGGCTGCCGTCATCGTTTACGAAAATACATACCGCTTCGCAGCCATTGGCAGTTTTAGCGGTTTTTTCCGTCAGCAGAAAGTCAAAAAATTCCAGCTCAAAGCCAAAGGACTCGTTCACCTGTTGCAGGTACTTCTTGTCGTACTGTT  >  NC_000913/1442560‑1442818

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑