Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,762,564	T→C	A482A (GCA→GCG)	sufB ←	Fe‑S cluster scaffold complex subunit SufB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,762,564	0	T	C	100.0%	122.6 / NA	34	A482A (GCA→GCG)	sufB	Fe‑S cluster scaffold complex subunit SufB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (23/11);  total (23/11)

AACGTCGAGGCTTAATCCGCGCAGGATAGCTTTATCTTCCACGCTGACGTGTAAATCTTTAATACTTAACATGTTTATTCCTTATCCGACGCTGTGTTCAAGACTGATGGCGAGGAGTTTTTGTGCTTCAACGGCAAATTCCAACGGCAGCTCCGAGAACACGTCTTTGCAGAAACCGTTAACAATCATCGAGATGGCGTCTTCTTCGCTGATCCCGCGTTGCAGGCAGTAAAACAGTTGATCTTCACCAAT  >  NC_000913/1762441‑1762692                                                                                                                            |                                                                                                                                 aaCGTCGAGGCTTAATCCGCGCAGGATAGCTTTATCTTCCACGCTGACGTGTAAATCTTTAATACTTAACATGTTTATTCCTTATCCGACGCTGTGTTCAAGACTGATGGCGAGGAGTTTTTGCGCTTCAACg                                                                                                                         >  2:106185/1‑133 (MQ=255)      cGAGGCTTAATCCGCGCAGGATAGCTTTATCTTCCACGCTGACGTGTAAATCTTTAATACTTAACATGTTTATTCCTTATCCGACGCTGTGTTCAAGACTGATGGCGAGGAGTTTTTGCGCTTCAACGGCaaa                                                                                                                    >  1:353555/1‑133 (MQ=255)                 ccGCGCAGGATAGCTTTATCTTCCACGCTGACGTGTAAATCTTTAATACTTAACATGTTTATTCCTTATCCGACGCTGTGTTCAAGACTGATGGCGAGGAGTTTTTGCGCTTCAACGGCAAATTCCAACGGCa                                                                                                         <  1:135183/133‑1 (MQ=255)                         gATAGCTTTATCTTCCACGCTGACGTGTAAATCTTTAATACTTAACATGTTTATTCCTTATCCGACGCTGTGTTCAAGACTGATGGCGAGGAGTTTTTGCGCTTCAACGGCAAATTCCAACGGCAGCTCCgag                                                                                                 >  1:513649/1‑133 (MQ=255)                                         aCGCTGACGTGTAAATCTTTAATACTTAACATGTTTATTCCTTATCCGACGCTGTGTTCAAGACTGATGGCGAGGAGTTTTTGCGCTTCAACGGCAAATTCCAACGGCAGGTCCAAGTACACGTCTTTGCAGa                                                                                 >  1:254443/1‑133 (MQ=255)                                              gACGTGTAAATCTTTAATACTTAACATGTTTATTCCTTATCCGACGCTGTGTTCAAGACTGATGGCGAGGAGTTTTTGCGCTTCAACGGCAAATTCCAACGGCAGCTCCGAGAACACGTCTTTGCAGAAACCg                                                                            >  1:560581/1‑133 (MQ=255)                                               aCGTGTAAATCTTTAATACTTAACATGTTTATTCCTTATCCGACGCTGTGTTCAAGACTGATGGCGAGGAGTTTTTGCGCTTCAACGGCAAATTCCAACGGCAGCTCCGAGAACACGTCTTTGCAGa                                                                                 <  2:527466/127‑1 (MQ=255)                                               aCGTGTAAATCTTTAATACTTAACATGTTTATTCCTTATCCGACGCTGTGTTCAAGACTGATGGCGAGGAGTTTTTGCGCTTCAACGGCAAATTCCAACGGCAGCTCCGAGAACACGTCTTTGCAGa                                                                                 >  1:527466/1‑127 (MQ=255)                                                  tgtAAATCTTTAATACTTAACATGTTTATTCCTTATCCGACGCTGTGTTCAAGACTGATGGCGAGGAGTTTTTGCGCTTCAACGGCAAATTCCAACGGCAGCTCCGAGAACACGTCTTTGCAGAAACCGTTaa                                                                        <  2:353555/133‑1 (MQ=255)                                                                   tAACATGTTTATTCCTTATCCGACGCTGTGTTCAAGACTGATGGCGAGGAGTTTTTGCGCTTCAACGGCAAATTCCAACGGCAGCTCCGAGAACACGTCTTTGCAGAAACCGTTAACAATCATCGAGATGGCg                                                       >  1:465581/1‑133 (MQ=255)                                                                    aaCATGTTTATTCCTTATCCGACGCTGTGTTCAAGACTGATGGCGAGGAGTTTTTGCGCTTCAACGGCAAATTCCAACGGCAGCTCCGAGAACACGTCTTTGCAGAAACCGTTAACAATCATCGAGATGGCGt                                                      >  2:486027/1‑133 (MQ=255)                                                                    aaCATGTTTATTCCTTATCCGACGCTGTGTTCAAGACTGATGGCGAGGAGTTTTTGCGCTTCAACGGCAAATTCCAACGGCAGCTCCGAGAACACGTCTTCGCAAAAACCGTTAACAATCATAGAGATGGCGt                                                      >  2:241011/1‑133 (MQ=255)                                                                        tGTTTATTCCTTATCCGACGCTGTGTTCAAGACTGATGGCGAGGAGTTTTTGCGCTTCAACGGCAAATTCCAACGGCAGCTCCGAGAACACGTCTTTGCAGAAACCGTTAACAATCATCGAGATGGCGtcttc                                                  <  2:513649/133‑1 (MQ=255)                                                                          tttATTCCTTATCCGACGCTGTGTTCAAGACTGATGGCGAGGAGTTTTTGCGCTTCAACGGCAAATTCCAACGGCAGCTCCGAGAACACGTGTTTGCAGAGACCGTTAGCAATCATCAAGATGGCGTcttctt                                                >  2:177930/1‑133 (MQ=255)                                                                                  ttATCCGACGCTGTGTTCAAGACTGATGGCGAGGAGTTTTTGCGCTTCAACGGCAAATTCCAACGGCAGCTCCGAGAACACGTCTTTGCAGAAACCGTTAACGATCATCGCGATCGCGTCTTCTACGCTGATc                                        >  1:242681/1‑133 (MQ=255)                                                                                   tATCCGACGCTGTGTTCAAGACTGATGGCGAGGAGTTTTTGCGCTTCAACGGCAAATTCCAACGGCAGCTCCGAGAACACGTCTTTGCAGAAACCGTTAACAATCATCGAGATGGCGTCTTCTTCGCTGATcc                                       >  2:573736/1‑133 (MQ=255)                                                                                   tATCCGACGCTGTGTTCAAGACTGATGGCGAGGAGTTTTTGCGCTTCAACGGCAAATTCCAACGGCAGCTCCGAGAACACGTCTTTGCAGAAACCGTTAACAATCATCGAGATGGCGTCTTCTTCGCTGATcc                                       >  2:614480/1‑133 (MQ=255)                                                                                    aTCCGACGCTGTGTTCAAGACTGATGGCGAGGAGTTTTTGCGCTTCAACGGCAAATTCCAACGGCAGCTCCGAGAACACGTCTTTGCAGAAACCGTTAACAATCATCGAGATGGCGTCTTCTTCGCTGATccc                                      >  1:549920/1‑133 (MQ=255)                                                                                    aTCCGACGCTGTGTTCAAGACTGATGGCGAGGAGTTTTTGCGCTTCAACGGCAAATTCCAACGGCAGCTCCGAGAACACGTCTTTGCAGAAACCGTTAACAATCATCGAGATGGCGTCTTCTTCGCTGATccc                                      >  2:3945/1‑133 (MQ=255)                                                                                      ccGACGCTGTGTTCAAGACTGATGGCGAGGAGTTTTTGCGCTTCAACGGCAAATTCCAACGGCAGCTCCGAGAACACGTCTTTGCAGAAACCGTTAACAATCATCGAGATGGCGTCTTCTTCGCTGATCCcgc                                    <  2:465581/133‑1 (MQ=255)                                                                                        gACGCTGTGTTCAAGACTGATGGCGAGGAGTTTTTGCGCTTCAACGGCAAATTCCAACGGCAGCTCCGAGAACACGTCTTTGCAGAAACCGATAACAATCATCGGGATCGCGTCTTCGTCGCAGATCCCGCGt                                  >  2:172354/1‑133 (MQ=255)                                                                                         aCGCTGTGTTCAAGACTGATGGCGAGGAGTTTTTGCGCTTCAACGGCAAATTCCAACGGCAGCTCCGAGAACACGTCTTTGCAGAAACCGTTAACAATCATCGAGATGGCGTCTTCTTCGCTGATCCCGCGtt                                 >  1:133608/1‑133 (MQ=255)                                                                                          cGCTGTGTTCAAGACTGATGGCGAGGAGTTTTTGCGCTTAAACGGCAAATTCCAACGGCAGCTCCGAGAACACGTCTTTGCAGAAACCGTTAACAATCATCGAGATGGCGTCTTCTTCGCTGATCCCGCGGTg                                >  2:370641/1‑133 (MQ=255)                                                                                            cTGTGTTCAAGACTGATGGCGAGGAGTTTTTGCGCTTCAACGGCAAATTCCAACGGCAGCTCCGAGAACACGTCTTTGCAGAAACCGTTAACAATCATCGAGATGGCGTCTTCTTCGCTGATCCCGCGTTgca                              <  1:486027/133‑1 (MQ=255)                                                                                            cTGTGTTCAAGACTGATGGCGAGGAGTTTTTGCGCTTCAACGGCAAATTCCAACGGCAGCTCCGAGAACACGTCTTTGCAGAAACCGTTAACAATCATCGAGATGGCGTCTTCTTCGCTGATCCCGCGTTgca                              <  2:560581/133‑1 (MQ=255)                                                                                            cTGTGTTCAAGACTGATGGCGAGGAGTTTTTGCGCTTCAACGGCAAATTCCAACGGCAGCTCCGAGAACACGTCTTTGCAGAAACCGTTAACAATCATCGAGATGGCGTCTTCTTCGCTGATCCCGCGTTgca                              <  2:549920/133‑1 (MQ=255)                                                                                                         tGATGGCGAGGAGTTTTTGCGCTTCAACGGCAAATTCCAACGGCAGCTCCGAGAACACGTCTTTGCAGAAACCGTTAACAATCATCGAGATGGCGTCTTCTTCGCTGATCCCGCGTTgcaggcag                         <  1:476722/125‑1 (MQ=255)                                                                                                         tGATGGCGAGGAGTTTTTGCGCTTCAACGGCAAATTCCAACGGCAGCTCCGAGAACACGTCTTTGCAGAAACCGTTAACAATCATCGAGATGGCGTCTTCTTCGCTGATCCCGCGTTgcaggcag                         >  2:476722/1‑125 (MQ=255)                                                                                                         tGATGGCGAGGAGTTTTTGCGCTTCAACGGCAAATTCCAACGGCAGCTCCGAGAACACGTCTTTGCAGAAACCGTTAACAATCATCGAGATGGCGTCTTCTTAGCTGATCACGCGTTGCAGGCAGTAAAACAg                 >  1:163646/1‑133 (MQ=255)                                                                                                          gATGGCGAGGAGTTTTTGCGCTTCAACGGCAAATTCCAACGGCAGCTCCGAGAACACGTCTTTGCAGAAACCGTTAACAATCATCGAGATGGCGTCTTCTTCGCTGATCCCGCGTTGCAGGCAGTAAAACAGt                <  2:133608/133‑1 (MQ=255)                                                                                                           aTGGCGAGGAGTTTTTGCGCTTCAACGGCAAATTCCAACGGCAGCTCCGAGAACACGTCTTTGCAGAAACCGTTAACAATCATCGAGATGGCGTCTTCTTCGCTGATCCCGCGTTGCAGGCAGTAAAACAGtt               >  1:450650/1‑133 (MQ=255)                                                                                                           aTGGCGAGGAGTTTTTGCGCTTCAACGGCAAATTCCAACGGCAGCTCCGAGAACACGTCTTTGCAGAAACCGTTAACAATCATCGAGATGGCGTCTTCTTCGATGATCCCGCGTGGCAGGCAGTAAAACAGtt               >  1:235636/1‑133 (MQ=255)                                                                                                                 aggagTTTTTGCGCTTCAACGGCAAATTCCAACGGCAGCTCCGAGAACACGTCTTTGCAGAAACCGTTAACAATCATCGAGATGGCGTCTTCTTCGCTGATCCCGCGTTGCAGGCAGTAAAACAGTTGATCtt         <  1:106185/133‑1 (MQ=255)                                                                                                                        tttGCGCTTCAACGGCAAATTCCAACGGCAGCTCCGAGAACACGTCTTTGCAGAAACCGTTAACAATCATCGAGATGGCGTCTTCTTCGCTGATCCCGCGTTGCAGGCAGTAAAACAGTTGATCTTCACCAAt  >  1:156832/1‑133 (MQ=255)                                                                                                                            |                                                                                                                                 AACGTCGAGGCTTAATCCGCGCAGGATAGCTTTATCTTCCACGCTGACGTGTAAATCTTTAATACTTAACATGTTTATTCCTTATCCGACGCTGTGTTCAAGACTGATGGCGAGGAGTTTTTGTGCTTCAACGGCAAATTCCAACGGCAGCTCCGAGAACACGTCTTTGCAGAAACCGTTAACAATCATCGAGATGGCGTCTTCTTCGCTGATCCCGCGTTGCAGGCAGTAAAACAGTTGATCTTCACCAAT  >  NC_000913/1762441‑1762692

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑