| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,764,382 | T→C | D2G (GAC→GGC) | sufA ← | iron‑sulfur cluster insertion protein SufA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,764,382 | 0 | T | C | 95.5% | 71.4 / NA | 22 | D2G (GAC→GGC) | sufA | iron‑sulfur cluster insertion protein SufA |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (11/10); minor base G (1/0); total (12/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CCGGCTGCTTTGCCACCAGCTCACGGATGTGTATCGCCGCTGCGGGTGTCAGCGTTAAGCCTTGCCAGGCGAAATCTTGTGGGTTAAAGGTTCCTGAATGCATGTCCATCGATTTACCTCACTTCATCGCTTTCAGCGTATAACAGCATGTTAGTGATAATGATTATCAGTTCAACCCAGCAAACGCAGGGGCTTTACCGTAAAACATGCTTTTTGCCTGCTTTTAATAAGCAT > NC_000913/1764278‑1764511 | ccGGCTGCTTTGCCACCAGCTCACGGATGTGTATCGCCGCTGCGGGTGTCAGCGTTAAGCCTTGCCAGGCGAAATCTTGTGGGTTAAAGGTTCCTGAATGCATGCCCATCGATTTACCTCACTTCATCGCttt < 1:640477/133‑1 (MQ=255)ccGGCTGCTTTGCCACCAGCTCACGGATGTGTATCGCCGCTGCGGGTGTCAGCGTTAAGCCTTGCCAGGCGAAATCTTGTGGGTTAAAGGTTCCTGAATGCATGCCCATCGATTTACCTCACTTCATCGCttt < 2:134529/133‑1 (MQ=255) ctgctTTGCCACCAGCTCACGGATGTGTATCGCCGCTGCGGGTGTCAGCGTTAAGCCTTGCCAGGCGAAATCTTGTGGGTTAAAGGTTCCTGAATGCATGGCCATCGAGTTACCTCAATTCATCGCTTTCAGc > 2:261875/1‑133 (MQ=255) ctTTGCCACCAGCTCACGGATGTGTATCGCCGCTGCGGGTGTCAGCGTTAAGCCTTGCCAGGCGAAATCTTGTGGGTTAAAGGTTCCTGAATGCATGCCCATCGATTTACCTCACTTCATCGCTTTCAGCGta > 2:374777/1‑133 (MQ=255) accaGCTCACAGATGCGTATCGCCACCGCGGGTGTCAGCGTAAAGCCTTGCCAGGCGAAATCTTGTGGGTTAAAGGTTCCTGAATGCATGCCCATCGATTTACCTCACTTCATCGCTTTCAGCGTATAACAGc < 2:293735/133‑1 (MQ=255) aGCTCACGGATGTGTATCGCCGCTGCGGGTGTCAGCGTTAAGCCTTGCCAGGCGAAATCTTGTGGGTTAAAGGTTCCTGAATGCATGCCCATCGATTTACCTCACTTCATCGCTTTCAGCGTATAACAGCATg > 2:106425/1‑133 (MQ=255) ggATGTGTATCGCCGCTGCGGGTGTCAGCGTTAAGCCTTGCCAGGCGAAATCTTGTGGGTTAAAGGTTCCTGAATGCATGCCCATCGATTTACCTCACTTCATCGCTTTCAGCGTATAACAGCATGTTAGTGa < 1:106425/133‑1 (MQ=255) ggATGTGTATCGCCGCTGCGGGTGTCAGCGTTAAGCCTTGCCAGGCGAAATCTTGTGGGTTAAAGGTTCCTGAATGCATGCCCATCGATTTACCTCACTTCATCGCTTTCAGCGTATAACAGCATGTTAGTGa < 1:408515/133‑1 (MQ=255) cgccgcTGCGGGTGTCAGCGTTAAGCCTTGCCAGGCGAAATCTTGTGGGTTAAAGGTTCCTGAATGCATGCCCATCGATTTACCTCACTTCATCGCTTTCAGCGTATAACAGCATGTTAGTGATAATGATTAt < 1:638062/133‑1 (MQ=255) ccgcTGCGGGTGTCAGCGTTAAGCCTTGCCAGGCGAAATCTTGTGGGTTAAAGGTTCCTGAATGCATGCCCATCGATTTACCTCACTTCATCGCTTTCAGCGTATAACAGCATGTTAGTGATAATGATTATCa > 1:490584/1‑133 (MQ=255) ccgcTGCGGGTGTCAGCGTTAAGCCTTGCCAGGCGAAATCTTGTGGGTTAAAGGTTCCTGAATGCATGCCCATCGATTTACCTCACTTCATCGCTTTCAGCGTATAACAGCATGTTAGTGATAATGATTATCa > 2:11925/1‑133 (MQ=255) ccgcTGCGGGTGTCAGCGTTAAGCCTTGCCAGGCGAAATCTTGTGGGTTAAAGGTTCCTGAATGCATGCCCATCGATTTACCTCACTTCATCGCTTTCAGCGTATAACAGAATGGTAGTGATAAGGATTATCa > 1:179011/1‑133 (MQ=255) ccgcTGCGGGTGTCAGCGTTAAGCCTTGCCAGGCGAAATCTTGTGGGTTAAAGGTTCCTGAATGCATGCCCATCGATTTACCTCACTTCATCGCTTTCAGCGAATAACAGTATGGTAGTGATAAAGATTATCa > 1:182087/1‑133 (MQ=255) cTGCGGGTGTCAGCGTTAAGCCTTGCCAGGCGAAATCTTGTGGGTTAAAGGTTCCTGAATGCATGCCCATCGATTTACCTCACTTCATCGCTTTCAGCGTATAACAGCATGTTAGTGATAATGATTATCAGtt > 1:104918/1‑133 (MQ=255) cTGCGGGTGTCAGCGTTAAGCCTTGCCAGGCGAAATCTTGTGGGTTAAAGGTTCCTGAATGCATGCCCATCGATTTACCTCACTTCATCGCGTTCAGCGTCTAAAAGCCTGTTAGTGGTAATGATTATCAGtt > 2:308649/1‑133 (MQ=255) ccTTGCCAGGCGAAATCTTGTGGGTTAAAGGTTCCTGAATGCATGCCCATCGATTTACCTCACTTCATCGCTTTCAGCGTATAACAGCATGTTAGTGATAATGATTATCAGTTCAACCCAGCa > 1:105193/1‑123 (MQ=255) ccTTGCCAGGCGAAATCTTGTGGGTTAAAGGTTCCTGAATGCATGCCCATCGATTTACCTCACTTCATCGCTTTCAGCGTATAACAGCATGTTAGTGATAATGATTATCAGTTCAACCCAGCa < 2:105193/123‑1 (MQ=255) tGCCAGGCGAAATCTTGTGGGTTAAAGGTTCCTGAATGCATGCCCATCGATTTACCTCACTTCATCGCTTTCAGCGTATAACAGCATGTTATTGATAATGATTATCAGATCAACCCACCAAACGCAGGGGCtt > 2:276865/1‑133 (MQ=255) tGCCAGGCGAAATCTTGTGGGTTAAAGGTTCCTGAATGCATGCCCATCGATTTACCTCACTTCATCGCTTTCAGCGTATAACAGCATGTTAGTGATAATGATTATCAGTTCAACCCAGCAAACGCAGGGGCtt < 2:490584/133‑1 (MQ=255) aaaGGTTCCTGAATGCATTCCCCTCGATTTCCCTCACTTCATCGCTTTCAGCGTATAACAGCATGTTAGTGATAATGATTATCAGTTCAACCCAGCAAACGCAGGGGCTTTACCGTAAAACATGCTTTTTGcc < 1:308649/133‑1 (MQ=255) gAATGCATGCCCATCGATTTACCTCACTTCATCGCTTTCAGCGTATAACAGCATGTTAGTGATAATGATTATCAGTTCAACCCAGCAAACGCAGGGGCTTTACCGTAAAACATGCTTTTTGCCTGCTTTtaat > 1:509700/1‑133 (MQ=255) aTGCCCATCGATTTATCTCACTTCATCGCTATCAGCGTATAACAGCATGTTAGTGATAATGATTATCAGTTCAACCCAGCAAACGCAGGGGCTTTACCGTAAAACATGCTTTTTGCCTGCTTTTAATAAGCAt < 1:222426/133‑1 (MQ=255) | CCGGCTGCTTTGCCACCAGCTCACGGATGTGTATCGCCGCTGCGGGTGTCAGCGTTAAGCCTTGCCAGGCGAAATCTTGTGGGTTAAAGGTTCCTGAATGCATGTCCATCGATTTACCTCACTTCATCGCTTTCAGCGTATAACAGCATGTTAGTGATAATGATTATCAGTTCAACCCAGCAAACGCAGGGGCTTTACCGTAAAACATGCTTTTTGCCTGCTTTTAATAAGCAT > NC_000913/1764278‑1764511 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |