Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,850,185 T→C V450A (GTT→GCT)  sppA → protease IV, a signal peptide peptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,850,1850TC96.7% 101.0 / NA 31V450A (GTT→GCT) sppAprotease IV, a signal peptide peptidase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (18/11);  minor base G (0/1);  total (19/12)
Fisher's exact test for biased strand distribution p-value = 4.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACTGGATTTCCACGCCAGCTAATTACATTGTGGCTAACCCCAGCACCCTGACCGGTTCTATCGGTATCTTCGGCGTGATCACCACCGTAGAAAATAGTCTGGATTCGATTGGTGTTCATACTGATGGTGTCTCAACTTCACCGCTGGCGGATGTTTCTATCACCAGGGCACTGCCGCCGGAAGCGCAGCTGATGATGCAGTTAAGCATTGAGAATGGCTATAAACGCTTTATCACGCTGGTTGC  >  NC_000913/1850071‑1850314
                                                                                                                  |                                                                                                                                 
aCTGGATTTCCACGCCAGCTAATTACATTGTGGCTAACCCCAGCACCCTGACCGGTTCTATCGGTATCTTCGGCGTGATCACCACCGTAGAAAATAGTCTGGATTCGATTGGTGCTCCTACTGATGGTGtctc                                                                                                                 >  1:248761/1‑133 (MQ=255)
aCTGGATTTCCACGCCAGCTAATTACATTGTGGCTAACCCCAGCACCCTGACCGGTTCTATCGGTATCTTCGGCGTGATCACCACCGTAGAAAATAGTCTGGATTCGATTGGTGCTCATACTGATGGTGtctc                                                                                                                 <  2:248761/133‑1 (MQ=255)
    gATTTCCACGCCAGCTAATTACATTGTGGCTAACCCCAGCACCCTGACCGGTTCTATCGGTATCTTCGGCGTGATCACCACCGTAGAAAATAGTCTGGATTCAATTGGTGATCAGACGGATGGTGTCTCAACt                                                                                                             >  1:203074/1‑133 (MQ=255)
                         cATTGTGGCTAACCCTAGCACCCTGACCTGTTCTATCGGTATCTTCGGCGTGATCACCACCGTAGAAAATAGTCTGGATTCGATTGGTGCTCATACTGATGGTGTCTCAACTTCACCGCTGGCGGATGTTTCt                                                                                        <  2:292207/133‑1 (MQ=255)
                            tgtgGCTAACCCCAGCACCCTGACCGGTTCTATCGGTATCTTCGGCGTGATCACCACCGCAGAAAGTAGTCTGGATTCGATTGGTTCTCATACTGATGGTGTCTCAACTTCACCGCTGGGGGATGTTTCTATc                                                                                     >  1:341867/1‑133 (MQ=255)
                             gtgGCTAACCCCAGCACCCTGACCGGTTCTATCGGTATCTTCGGCGTGATCACCACCGTAGAAAATAGTCTGGATTCGATTGGTGCTCATACTGATGGTGTCTCAACTTCACCGCTGGCGGATGTTTCTATCa                                                                                    >  2:48469/1‑133 (MQ=255)
                               ggCTAACCCCAGCACCCTGACCGGTTCTATCGGTATCTTCGGCGTGATCACCACCGTAGAAAATAGTCTGGATTCGATTGGTGCTCATACTGATGGTGTCTCAACTTCACCGCTGGCGGATGTTTCTATCAcc                                                                                  >  1:618587/1‑133 (MQ=255)
                                gCTAACCCCAGCACCCTGACCGGTTCTATCGGTATCTTCGGCGTGATCACCACCGTAGAAAATAGTCTGGATTCGATTGGTGCTCATACTGATGGTGTCTCAACTTCACCGCTGGCGGATGTTTCTATCACCa                                                                                 >  2:86445/1‑133 (MQ=255)
                                gCTAACCCCAGCACCCTGACCGGTTCTATCGGTATCTTCGGCGTGATCACCACCGTAGAAAATAGTCTGGATTCGATTGGTGCTCATACTGATGGTGTCTCAACTTCACCCCTGACGGATGTTTATATCACCa                                                                                 >  1:250521/1‑133 (MQ=255)
                                 cTAACCCCAGCACCCTGACCGGTTCTATCGGTATCTTCGGCGTGATCACCACCGTAGAAAATAGTCTGGATTCGATTGGTGCTCATACTGATGGTGTCTCAACTTCACCGCTGGCGGATGTTTCTATCACCAg                                                                                >  2:566514/1‑133 (MQ=255)
                                       ccAGCACCCTGACCGCTTCTATCGGTATCTACGGCGTGATCACCACCGTAGAAAATAGTCTGGATTCGATTGGTGCTCATACTGATGGTGTCTCAACTTCACCGCTGGCGGATGTTTCTATCACCAGGGCACt                                                                          <  1:234915/133‑1 (MQ=255)
                                          gCACCCTGACCGGTTCTATCGGTATCTTCGGCGTGATCACCACCGTAGAAAATAGTCTGGATTCGATTGGTGCTCATACTGATGGTGTCTCCACTTCACCCCTGCCGGTTGTTTCTATCACCCGGGCACTgcc                                                                       >  2:202368/1‑133 (MQ=255)
                                          gCACCCTGACCGGTTCTATCGGTATCTTCGGCGTGATCACCACCGTAGAAAATAGTCTGGATTCGATTGGTGCTCATACTGATGGTGTCTCAACTTCACCGCAGGCGGATATTTCTAGCACCAGGGCACTgcc                                                                       >  1:178902/1‑133 (MQ=255)
                                           cACCCTGACCGGTTCTATCGGTATCTTCGGCGTGATCACCACCGTAGAAAATAGTCTGGATTCGATTGGTGCTCATACTGATGGTGTCTCAACTTCACCGCTGGCGGATGTTTCTATCACCAGGGCACTgccg                                                                      >  1:55339/1‑133 (MQ=255)
                                                  acctgTTCTATCGGTATCTTCGGCGTGATCACCACCGTAGAAAATAGTCTGGATTCGATTGGTGCTCATACTGATGGTGTCTCAACTTCACCGCTGGCGGATGTTTCTATCACCAGGGCACTGCCGCCGGAAg                                                               >  1:634851/5‑133 (MQ=255)
                                                   ccGGTTCTATTGGTAGCTTCGGCGCGATCACCCCCGTAGAACATAGTCTGGATTCGATTGGTGCTCATACTGATGGTGTCTCAACTTCACCGCTGGCGGATGTTTCTATCACCAGGGCACTGCCGCCGGAAgc                                                              <  2:178902/133‑1 (MQ=255)
                                                   ccGGTTCTATCGGTATCTTCGGCGTGATCACCACCGTAGAAAATAGTCTGGATTCGATTGGTGCTCATACTGATGGTGTCTCAACTTCACCGCTGGCGGATGTTTCTATCACCAGGGCACTGCCGCCGGAAgc                                                              <  2:611160/133‑1 (MQ=255)
                                                      gTTCTATCGGTATCTTCGGCGTGATCACCACCGTAGAAAATAGTCTGGATTCGATTGGTGCTCATACTGATGGTGTCTCAACTTCACCGCTGGCGGATGTTTCTATCACCAGGGCACTGCCGCCGGAAGCGCa                                                           <  2:618587/133‑1 (MQ=255)
                                                      gTTCTATCGGGATCTGCGGCGTGATCACCACCTTAGAAAATGGTCTGGATTCGATTGGTGCTCATACTGATGGTGTCTCAACTTCACCGCTGGCGGATGTTTCTATCACCAGGGCACTGCCGCCGGAAGCGCa                                                           <  2:230824/133‑1 (MQ=255)
                                                              ggTATCTTCGGCGTGATCACCACCGTAGAAAATAGTCTGGATTCGATTGGTGCTCATACTGATGGTGTCTCAACTTCACCGCTGGCGGATGTTTCTATCACCAGGGCACTGCCGCCGGAAGCGCAGCtgatga                                                   <  1:559296/133‑1 (MQ=255)
                                                              ggTATCTTCGGCGTGATCACCACCGTAGAAAATAGTCTGGATTCGATTGGTGCTCATACTGATGGTGTCTCAACTTCACCGCTGGCGGATGTTTCTATCACCAGGGCACTGCCGCCGGAAGCGCAGCtgatga                                                   <  1:524430/133‑1 (MQ=255)
                                                                 aTCTTCGGCGTGATCACCACCGTAGAAAATAGTCTGGATTCGATTGGTGCTCATACTGATGGTGTCTCAACTTCACCGCTGGCGGATGTTTCTATCACCAGGGCTCTGGCGCCGGAAGCGCAGCTGATGATGc                                                >  2:174060/1‑133 (MQ=255)
                                                                     tCGGCGTGATCACCGCCGTAGAAAATAGTCTGGATTCGATTGGTGCTCATACTGATGGTGTCTCATCTTCACCGCTGGCGGATGTTTCTATCACCAGGGCACTGCCGCCGGAAGCGCAGCTGATGATGCAGtt                                            <  2:341867/133‑1 (MQ=255)
                                                                      cGGCGTGATCACCACCGTAGAAAATAGTCTGGATTCGATTGGTGCTCATACTGATGGTGTCTCAACTTCACCGCTGGCGGATGTTTCTATCACCAGGGCACTGCCGCCGGAAGCGCAGCTGATGATGCAGTTa                                           >  2:588622/1‑133 (MQ=255)
                                                                         cGTGATCACCCCCGTCGAAAATAGACTGGATTCGATTGGTGGTCATACTGATGGTGTCTCAACTTCACCGCTGGCGGATGTTTCTATCACCAGGGCACTGCCGCCGGAAGCGCAGCTGATGATGCAGTTAAGc                                        <  2:213584/133‑1 (MQ=255)
                                                                          gTGATCACCACCGTAGAAAATAATCTGGATACGATTGGTGCTCATACTGATGGTGTCTCAACTTCACCGCTGGCGGATGTTTCTATCACCAGGGCACTGCCGCCGGAAGCGCAGCTGATGATGCAGTTAAGCa                                       <  1:202368/133‑1 (MQ=255)
                                                                                accaccGTAGAAAATTGTCTGGATCCGATTGGTGCTCATACCGATGGTGTCTCAACTTCACCGCTGGCGGATGTTTCTATCACCAGGGCACTGCCGCCGGAAGCGCAGCTGATGATGCAGTTAAGCATTgaga                                 <  2:250521/133‑1 (MQ=255)
                                                                                       tAGAAAATAGTCTGGATTCGATTGGTGCTCATACTGATGGTGTCTCAACTTCACCGCTGGCGGATGTTTCTATCACCAGGGCACTGCCGCCGGAAGCGCAGCTGATGATGCAGTTAAGCATTGAGAATGGCta                          >  1:595424/1‑133 (MQ=255)
                                                                                       tAGAAAATAGTCTGGATTCGATTGGTGCTCATACTGATGGTGTCTCAACTTCACCGCTGGCGGATGTTTCTATCACCAGGGCACTGCCGCCGGAAGCGCAGCAGATGATGCAGTGAAGCATTGACAATGGCta                          >  1:236489/1‑133 (MQ=255)
                                                                                               aGTCTGGATTCGATTGGTGCTCATACTGATGGTGTCTCAACTTCACCGCTGGCGGATGTTTCTATCACCAGGGCACTGCCGCCGGAAGCGCAGCTGATGATGCAGTTAAGCATTGAGCATGGCTGTAAACGCt                  >  1:295903/1‑133 (MQ=255)
                                                                                                   tGGATTCGATTGGTGCTCATACTGATGGTGTCTCAACTTCACCGCTGGCGGATGTTTCTATCACCAGGGCACTGCCGCCGGAAGCGCAGCTGATGATGCAGTAAAGCATTGAGAGTGGCTATAAACGCTTTAt              >  1:200459/1‑133 (MQ=255)
                                                                                                    ggATTCGATTGGTGCTCATACTGATGGTGTCTCAACTTCACCGCTGGCGGATGTTTCTATCACCAGGGCACTGCCGCCGGAAGCGCAGCTGCTGATGCAGGTAAACATAGAGAATGGGTATAGACGCTTTATc             >  2:196191/1‑133 (MQ=255)
                                                                                                               gtgcTCATACTGATGGTGTCTCAACTTCACCGCTGGCGGATGTTTCTATCACCAGGGCACTGCCGCCGGAAGCGCAGCTGATGATGCAGTTAAGCATTGAGAATGGCTATAAACGCTTTATCACGCTGGTTGc  >  2:451446/1‑133 (MQ=255)
                                                                                                                  |                                                                                                                                 
ACTGGATTTCCACGCCAGCTAATTACATTGTGGCTAACCCCAGCACCCTGACCGGTTCTATCGGTATCTTCGGCGTGATCACCACCGTAGAAAATAGTCTGGATTCGATTGGTGTTCATACTGATGGTGTCTCAACTTCACCGCTGGCGGATGTTTCTATCACCAGGGCACTGCCGCCGGAAGCGCAGCTGATGATGCAGTTAAGCATTGAGAATGGCTATAAACGCTTTATCACGCTGGTTGC  >  NC_000913/1850071‑1850314

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑